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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for Cytosolic arginine sensor for mTORC1 subunit 2 CASTOR2 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Alexa Flour 594 Cholera toxin beta subunit conjugate (CTb-594, Invitrogen; 0.5% w/v, 400–500 nl) was injected in dHPC of CD-1 mice ...
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bioRxiv - Biophysics 2023Quote: ... Alexa Fluor 488 conjugated with cholera toxin B subunit (CTxB-Alexa 488) was obtained from Molecular Probes, Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: For striatal injections of cholera toxin subunit-B (CTB) Alexa fluor-conjugated 488 (Invitrogen Cat. No C34775), unilateral injections were performed into the ipsilateral dorsal striatum to the 6OHDA lesion side (AP +0.5 mm ...
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bioRxiv - Microbiology 2022Quote: ... and plasmids encoding two vaccinia virus capping enzyme subunits (pCAG-D1R and pCAG-D12L −0.8μg each) using 16μL Lipofectamine 2000 (Invitrogen) per transfection reaction in a total volume of 200μL of Opti-MEM (Gibco) ...
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bioRxiv - Molecular Biology 2021Quote: ... by passaging at least 9 times in Dulbecco’s Modified Eagle Medium (DMEM) for SILAC lacking lysine and arginine (Thermo Scientific) containing 10 % dialyzed fetal bovine serum (Thermo Scientific ...
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bioRxiv - Cancer Biology 2020Quote: A synthetic peptide labeled with heavy arginine (13C, 15N) was formulated for the MDSTEPPYSQK(me3)R (eEF1A1_K318) peptide (HeavyPeptide AQUA custom synthesis service, Life Technologies). Synthetic peptides were spiked into the WT and SETD2-KO lysates from the HKC and 786-O cells ...
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bioRxiv - Biochemistry 2021Quote: ... 1.15 mM) or with the same concentrations of amino acid isotopes 2H4-Lysine (Lys4) and 13C6--Arginine (Arg6) (Thermo Scientific). The medium was supplemented with 50 μM β-mercaptoethanol ...
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bioRxiv - Biochemistry 2023Quote: SILAC labeling was performed by growing cells for at least eight passages in lysine- and arginine-free SILAC medium (DMEM, Invitrogen) supplemented with 10% dialyzed fetal calf serum (Thermo Fisher) ...
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bioRxiv - Systems Biology 2024Quote: ... medium was replaced 24h prior to sampling time by either DMEM-F12 for SILAC medium (Fischer Scientific) supplemented with heavy L-lysine and heavy L-arginine as well as E8 supplements (Gibco) and 0.1% BSA for iPSCs and non-plated EBs and with DMEM for SILAC medium (Fischer Scientific ...
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bioRxiv - Cancer Biology 2024Quote: SILAC labeling was performed by growing cells for at least five passages in lysine- and arginine-free SILAC medium (RPMI or DMEM, Invitrogen) supplemented with 10% dialyzed fetal calf serum (Gemini ...
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bioRxiv - Cancer Biology 2024Quote: Absolute quantification of peptide NADK arginine methylation was performed using a Q Exactive™ mass spectrometer (MS, ThermoFisher Scientific, USA) connected to a Dionex UltiMate 3000 RS UPLC system ...
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bioRxiv - Cell Biology 2022Quote: ... FITC-Bm-SA (FITC-labeled toad B. maxima serum albumin (24)) or 20 μg/mL Ovalbumin-DQ (ThermoFisher, Cat D12053) in the dark at 26°C for 15 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Cell associated immunoglobulin was detected with an appropriate FITC-labelled secondary antibody and fluorescence measured using an Attune NXT cytometer (Thermofisher, UK). Data are shown as relative fluorescence intensities calculated as a percentage of the negative control fluorescence.
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bioRxiv - Plant Biology 2020Quote: ... Detection was carried out with an FITC-coupled antibody to rabbit IgG (Molecular Probes; 1:100 dilution, 37°C, 40 min) in 0.5% BSA in PBS ...
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bioRxiv - Immunology 2022Quote: ... cells were stained for 20 mins at RT with an anti-human FITC-labelled secondary antibody (dilution 1:100) (Thermo Fisher) for A32 detection ...
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bioRxiv - Microbiology 2022Quote: ... MDMs were then permeabilized and stained using KC57 FITC-conjugated anti-Gag antibody (1/200 dilution) and phalloidin-Alexa Fluor 647 (Molecular Probes) diluted in permeabilization buffer for 1 h ...
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bioRxiv - Immunology 2021Quote: Neutrophil samples were stained with anti-MPO-FITC antibody and mounted in DAPI-containing ProLong Gold Antifade Mountant (Thermo Fisher Scientific). AIM2 and IFI16 proteins were expressed ...
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bioRxiv - Genomics 2022Quote: ... followed by a 1:100 dilution of Anti-mouse CD45 antibody conjugated to FITC (Thermo Fisher Scientific, Cat#: 11-0451-81) and 1:100 dilution of Anti-mouse EpCAM antibody conjugated to eFluor450 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated for 1h with anti-human IgG goat antibody conjugated to FITC or Alexa Fluor 488 (H10301; A-11013, Invitrogen, USA) diluted 1/500 in PBS ...
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bioRxiv - Genetics 2020Quote: ... cells were processed for intra-cellular HbF staining using Fetal Hemoglobin Monoclonal Antibody (HBF-1) conjugated to FITC (Thermo Fisher #MHFH01), and HbF+ cells were sorted by FACS as previously described6 ...
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bioRxiv - Microbiology 2022Quote: ... then stained with a Borrelia polyclonal antibody diluted to 1:50 in 1x PBS (ThermoFisher, Waltham, MA, USA; FITC-PA1-73005) according to a previously established protocol [31] ...
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bioRxiv - Genomics 2024Quote: ... Antibodies were added to stain surface markers for 30 min at 4°C (CD3 PE, Life Technologies; CD8 FITC, Life Technologies; CD11b AF700 ...
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bioRxiv - Immunology 2024Quote: ... or LAMP-1/CD107a monoclonal antibody specific for the lysosomal-associated membrane protein 1 (LAMP-1) of the parasitophorous vacuole (rat IgG2a FITC, Invitrogen, CA). Revelation was performed with 1.5 μg/ml streptavidin conjugated to Texas Red (Molecular Probes ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated for 1h with anti-human IgG goat polyclonal antibodies conjugated to FITC or Alexa Fluor 488 (H10301; A-11013, Invitrogen, USA) diluted 1/100 in FACS buffer ...
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bioRxiv - Bioengineering 2023Quote: ... wells were washed 3 times with PBS with 0.1% tween (PBST) and then incubated overnight at 4 °C with a human hemoglobin-FITC conjugated antibody (Life Technologies, A80134F). Samples were washed three times with PBST prior to imaging on a Keyence BZ-X800 microscope.
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bioRxiv - Microbiology 2024Quote: ... FITC-conjugated mouse anti-human CD4 (Clone OKT4) Antibody (1:500 dilution) and AquaVivid viability dye (Thermo Fisher Scientific, Cat# L43957) for 20 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... harvested cells were separated into nuclear and cytosolic fractions via NE-PER kit following the manufacturer’s instructions (ThermoFisher Scientific; Cat# 78833, Waltham, MA).
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bioRxiv - Neuroscience 2021Quote: ... was lysed in cytosolic extraction buffer and protease inhibitors included in the Sub-Cellular Protein Fractionation Kit for Tissues (Thermo Scientific, Waltham, MA) using the PowerGen 125 handheld homogenizer (Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... of nuclear or 40 μg of cytosolic proteins were resolved by SDS-polyacrylamide gel electrophoresis in a 12% polyacrylamide gel (NuPAGE Gel, NP0335BOX; Thermo Fisher Scientific, USA). Proteins were transferred to nitrocellulose membranes (Thermo Fisher Scientific ...
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A DNA-based optical nanosensor for in vivo imaging of acetylcholine in the peripheral nervous systembioRxiv - Bioengineering 2020Quote: The UV-Vis spectrum of DNA scaffold/sensors was measured by a NanoDrop™ 2000c Spectrophotometer (Thermo Scientific). Native PAGE was conducted to characterize DNA nanostructures by using 4% TBE gel and 1x TBE buffer at a constant voltage of 200 V (electrophoresis system Thermo Scientific ...
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bioRxiv - Systems Biology 2020Quote: The singlet oxygen generator (SOG) thiorhodamine was mixed with 1 μM Singlet Oxygen Sensor Green (Invitrogen, cat: S36002) in phosphate buffered saline at increasing D2O/H2O ratios and illuminated at a working distance of 30 cm with 590-nm Precision LED spotlights controlled by a BioLED Light Control Module (Mightex Systems ...
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bioRxiv - Biophysics 2022Quote: ... Rather than purified sensor,20 μl of a 1/10,000 dilution of carboxylate latex fluorescent beads (Fluospheres, Invitrogen) of either 20 nm (F-8786) ...
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bioRxiv - Biophysics 2020Quote: For monitoring the Ca2+cyt Jurkats were loaded with the cell permeable Ca2+ sensor Fluo-4 (Life Technologies) for 30 min in microscopy buffer in a final concentration of 1 μM ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1 mM MgCl2 containing 4.5 µM phosphate-binding protein labeled with a phosphate sensor (MDCC, Fisher Scientific) for intrinsic GTPase activity or containing 1 nM NF1-GAP for GAP mediated GTPase activity ...
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bioRxiv - Molecular Biology 2022Quote: ... Image-iT® Lipid Peroxidation Sensor (10 µM; Image-iT Lipid Peroxidation Kit – Thermo Fisher Scientific – ref C10445) was added ...
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bioRxiv - Genomics 2023Quote: Cell cycle analysis was performed using the Premo FUCCI Cell Cycle Sensor kit (Thermo Fisher Scientific; Cat # P36237) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... A549-ACE2 cells were transduced with the Premo Autophagy Tandem Sensor RFP-GFP-LC3B kit (P36239, Thermo Scientific) and in 24 hours ...
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bioRxiv - Cell Biology 2024Quote: ... Various stages of autophagy were monitored using Premo™ Autophagy Tandem Sensor RFP-GFP LC3B Kit (Life Technologies) according to the manufacturer’s instructions (see Supplemental Methods for details).
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bioRxiv - Immunology 2021Quote: ... anti-CD11b-FITC (#11-0112-85, Invitrogen, UK), DAPI (#32670 ...
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bioRxiv - Developmental Biology 2021Quote: ... FITC-Donkey anti-Chicken (Thermo Fisher SA1-72000) used at 1:400 ...
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bioRxiv - Molecular Biology 2020Quote: ... F-actin was detected with FITC-phalloidin (Invitrogen) and nuclei were visualised using DAPI ...
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bioRxiv - Molecular Biology 2020Quote: ... goat anti-bovine IgG conjugated to FITC (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... was incubated with FITC-labeled avidin (Life Technologies) for 1 h at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Annexin V/FITC apoptosis detection kit (ThermoFisher, USA) was used to stain HCT-116 cells for the assessment of viability and apoptosis ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stained with CD8a-FITC (Life Technologies), CD4-PE (Miltenyi Biotec ...
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bioRxiv - Systems Biology 2021Quote: ... anti-human FITC-IgG (Fc specific) (ThermoFisher Scientific) at a dilution of 1:400 and for 45 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Anti-mouse CD4 FITC (Invitrogen; #11-0041-85), Anti mouse CD25 PE (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... CD45-FITC (1:1000, Invitrogen 11-0451-82), ITAG7(a7)-APC (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... and FITC-conjugated anti-Eomes clone WD1928 (Invitrogen) were included whereas Biotin-conjugated antibodies anti-CD3 145-2C11 ...
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bioRxiv - Cancer Biology 2021Quote: ... conjugated with FITC were purchased from Thermo Fisher Scientific (Waltham ...