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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Cytosolic arginine sensor for mTORC1 subunit 2 CASTOR2 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Nuclear localised FRET barnase was generated by the addition of a N-terminal SV40 NLS sequence to the original cytosolic barnase using a synthesized gene cassette containing the relevant localization sequences (GeneArt (Thermofisher), Waltham ...
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bioRxiv - Developmental Biology 2023Quote: ... The cytosolic pool of SEMA6A-GFP or SEMA6AΔcyt-GFP was visualised using rabbit-anti-GFP (1:1000, A11122 Life Technologies) followed by 1 h incubation of Alexa Fluor-488-conjugated donkey-anti-rabbit secondary antibodies (1:1000 ...
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bioRxiv - Developmental Biology 2024Quote: ... and the cytosolic and membrane proteins were separated using the Mem-PER Plus Membrane Protein Extraction Kit (Thermo Fisher: 89842) with cOmplete™ Mini Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Molecular Biology 2023Quote: 5′-32P-labeled pre-miRNA was incubated in Xenopus cytosolic extract (10 µg of total protein) with RNase inhibitor (10U of RNaseOUT, Invitrogen) and carrier RNA (2 µg of yeast tRNA ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was then isolated from these pure cytosolic fractions using the PureLink Genomic DNA Mini Kit (Thermo Fisher Scientific, K182001). qPCR was performed on both whole cell extracts and cytosolic fractions using nuclear DNA primers (18S ...
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bioRxiv - Biophysics 2024Quote: ... replacing the caged dye in the HM with a small drop of 0.2-0.3 μl of a cytosolic fluorescent marker (3kD dextran conjugated to Alexa fluor 647 or Texas Red at concentrations of 3 mM; Invitrogen) that is applied from above just before electroporation to generate an inhomogeneous dye distribution around the animal during electroporation ...
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bioRxiv - Cell Biology 2024Quote: Different cells were harvested and fractionated to produce cytosolic and nuclear lysates using the NE-PER kit (Thermo Fisher Scientific). For whole cell lysates ...
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bioRxiv - Genomics 2024Quote: ... Cytosolic and nuclear protein extracts were prepared using Pierce NE-PER Nuclear and Cytoplasmic Extraction Reagent Kit (Thermo Scientific # 78833), as per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Cytosolic and nuclear extracts and acid-extracted histones were separated on 10% or 4-12% Bis-Tris gels (Life Technologies) and transferred to nitrocellulose membranes (0.2 μm ...
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bioRxiv - Neuroscience 2024Quote: ... from the cytosolic tdTomato as well as from Alexa488 or 594 Hydrazide (added to the intracellular solution, 10 µM; Invitrogen) fluorescence ...
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bioRxiv - Immunology 2024Quote: ... both NK- triggering 721.221 cells and bystander K562 cells were labeled with 1 µM of the cytosolic dye Calcein Red-Orange AM (Invitrogen). Simultaneously ...
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bioRxiv - Cell Biology 2024Quote: ... The concentrations of the cytosolic and chromatome extracts were determined using the Pierce BCA Protein Assay Kit (Thermo Scientific; #PIER23225).
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bioRxiv - Cancer Biology 2022Quote: ... Panel 2: (PE-Cf594 Ly6G, PerCP-Cy5.5 Ly6C, FITC CD11b) and Panel 3: Alexa fluor 594 anti-mCherry (ThermoFisher-M11240), Alexa fluor 647 CD34 (BD 560230) ...
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bioRxiv - Biophysics 2020Quote: ... Fluorescently labeled cholera toxin subunit B (catalog number: C34775, C34776, C34778) was purchased from ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... cells were co-stained with recombinant Cholera toxin subunit B conjugated to Fluor488 (Invitrogen C34775) at 1 μg/mL for less than 1 minute prior to fixing and staining for NINJ1 as described above ...
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bioRxiv - Neuroscience 2020Quote: ... 3ul 0.2% cholera toxin B subunit (CTB) conjugated Alexa 488 dye (CTB488, Life Technologies, C22841) was intravitreally injected into the left eye ...
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bioRxiv - Neuroscience 2021Quote: ... cholera-toxin subunit B (CTB) conjugated with Alexa fluorophore 488 (Thermo Fisher Scientific, Massachussetts, USA), 555 or 647 was unilaterally (left/right hemisphere ...
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bioRxiv - Neuroscience 2021Quote: ... we injected fluorescently-labeled cholera toxin subunit B (CTB, 500 µg / 100 µL, Molecular Probes, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... 1 ng Cholera Toxin subunit B (CT-B) (Recombinant Alexa Fluor 488 conjugate, Life Technologies) was injected into the hindbrain ventricle81 ...
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bioRxiv - Biophysics 2021Quote: ... and the specific Gα subunit were co-expressed in Spodoptera frugiperda (sf9) insect cells (Invitrogen) as previously described 52 ...
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bioRxiv - Immunology 2021Quote: ... ε-subunits and rapsyn-GFP were each cloned into pcDNA3.1-hygro plasmid vectors (Invitrogen, CA) and cDNA encoding human full-length MuSK was cloned into pIRES2-EGFP plasmid vector (Clontech) ...
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bioRxiv - Biochemistry 2022Quote: ... Alexafluor-594 tagged recombinant cholera toxin subunit B (CTB-red) was also purchased from Invitrogen Molecular Probes and used as per manufacturer’s recommendations (10 µM final concentration).
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bioRxiv - Neuroscience 2024Quote: Cholera Toxin Subunit B (CTB) conjugated with Alexa Fluor 488 (0.5mg/1ml PBS) (Molecular Probes) was injected into either proximal iliopsoas (IP) ...
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bioRxiv - Neuroscience 2024Quote: Cholera toxin subunit B (recombinant) (CTb) Alexa Fluor 488 (Cat#C34775, Therma Fisher Scientific, USA) and CTb Alexa Fluor 555 (Cat#CC34776 ...
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bioRxiv - Immunology 2022Quote: ... 2 h goat anti-rabbit IgG F(ab’)2-AF488 secondary antibody (Invitrogen), 15 min DAPI (1:1000) ...
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bioRxiv - Cancer Biology 2023Quote: Colon cancer cell lines were grown in arginine free media (Thermofisher, Cat # A2493901, US Bio Cat# D9803-07B) supplemented with 10% dialyzed FBS (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... cells washed 3 times with PBS and 5ml of serum-free DMEM deficient in lysine and arginine (Gibco) with (13C6)-labelled L-lysine (CK Isotopes Ltd ...
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bioRxiv - Neuroscience 2021Quote: ... or rabbit IgG antibody (2 μg, Thermo Fisher), as well as anti-S ...
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bioRxiv - Cell Biology 2020Quote: Anti-MAP2 antibody (microtubule associated protein 2; Thermofisher) was used to identify neurons ...
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bioRxiv - Microbiology 2022Quote: ... and the HA monoclonal antibody 2-2.2.14 (Invitrogen) were used at a 1:1000 & 1:2500 dilution ...
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bioRxiv - Immunology 2024Quote: ... and 2 μg/mL soluble αCD28 antibody (ThermoFisher). Ab-expanded populations were restimulated after 11 days using equivalent concentrations of each reagent ...
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bioRxiv - Immunology 2020Quote: ... Anti-mouse monoclonal antibodies targeting cell surface antigens included anti-GL7 (FITC, PE, eFluor660; clone GL 7; Thermo Fisher), anti-B220 (PE-Cy7 ...
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bioRxiv - Genomics 2022Quote: ... a 1:100 dilution of Anti-Mouse CD45 antibody conjugated to FITC (Thermo Fisher Scientific, Cat#: 11-0451-810) and a 1:400 dilution of Anti-Mouse Thy-1.2 antibody conjugated to APC (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... and stained using antibodies specific for intracellular markers including anti-Ki67-FITC (Invitrogen 14-5698-82: Clone: SolA15, eBioscience) (1:200) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2ry mouse-C5 antibody (Jacksons Lab, Maine, USA) was then incubated together with FITC-Phaloidine (Thermofisher Scientific, Massachusetts, USA). Nuclear DNA was counter-stained with Hoechst (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... and followed by the incubation with the secondary antibody (FITC-goat anti-rabbit IgG, 1:250 dilution, #L43001 Invitrogen) for 30 minutes at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Neutralization with sodium tetraborate was followed by incubation with anti-BrdU/FITC antibody (ThermoFisher, clone BU20A, #11-5071-42). Cells were stained with propidium iodide and treated with RNase A (VWR ...
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bioRxiv - Bioengineering 2024Quote: ... A volume of 25 µL (equivalent to 2.5 x 108 particles) was incubated with anti-mouse OX40L-FITC antibodies (Invitrogen anti-mouse OX40L Monoclonal Antibody (OX89)-FITC ...
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bioRxiv - Cancer Biology 2024Quote: Antibodies (Human Siglec-1, PE conjugated Anti-Human CD14, FITC conjugated Anti-Human CD16) were obtained from Thermo Fisher Inc ...
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bioRxiv - Cell Biology 2022Quote: ... parasites were labelled with the fluorescent lipid peroxidation sensor BODIPY581/591 C11 (Invitrogen, Thermo Fisher, USA) based on the method described by Fu et al ...
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bioRxiv - Cell Biology 2022Quote: ... parasites were labelled with the fluorescent lipid peroxidation sensor BODIPY581/591 C11 (Invitrogen, Thermo Fisher, USA) based on the method described by Fu et al ...
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bioRxiv - Synthetic Biology 2022Quote: ... Culture of transformed sensors (5 μL) was plated onto a 12-well plate (Thermo Fisher Scientific) for selection ...
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bioRxiv - Cancer Biology 2020Quote: ... the cells were harvested and subjected to nuclear and cytosolic fractionation via NE-PER kit (ThermoFisher Scientific; Cat# 78833, Waltham, MA). Nuclear protein was isolated and 100 μg of lysate was submitted to UAMS proteomics core facility.
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bioRxiv - Neuroscience 2021Quote: The whole extract and the cytosolic and synaptosome protein fractions were collected using the Syn-PER™ Synaptic Protein Extraction Reagent (ThermoFisher) following manufacture instructions ...
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bioRxiv - Microbiology 2022Quote: ... MYXV infection or Selinexor + MYXV and nuclear and cytosolic fractions were prepared using NE-PER nuclear and cytoplasmic extraction reagents (Thermo Scientific). Purity of the fractions was confirmed by western blot analysis for tubulin (cytoplasmic ...
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bioRxiv - Molecular Biology 2024Quote: ... or nuclear and cytoplasmic extraction reagents (for protein isolation from nuclear and cytosolic fractions) supplemented with protease and phosphatase inhibitors cocktails (ThermoFisher Scientific). Equal amounts of protein lysates (20 µg ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclear and cytosolic protein fractions from muscle biopsies were prepared using NE-PER™ Nuclear and Cytoplasmic Extraction Kit (Catalog. No 78835; ThermoFisher) according to the manufacturer instructions with the help of Dounce homogenizer.
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bioRxiv - Microbiology 2023Quote: ... for NLS-TurboID (nuclear fraction) and NES-TurboID (cytosolic fraction) and the Subcellular Protein Fractionation Kit (Thermo Scientific, Catalog Number 78840) for CD2AP-TurboID and CLASP11256−1538-TurboID (membrane fractions) ...
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bioRxiv - Cell Biology 2023Quote: ... or nuclear and cytoplasmic extraction reagents (for protein isolation from nuclear and cytosolic fractions) supplemented with protease and phosphatase inhibitors cocktails (ThermoFisher Scientific). Equal amounts of protein lysates (20 µg ...
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Cell-Type Specific Profiling of Histone Post-Translational Modifications in the Adult Mouse StriatumbioRxiv - Neuroscience 2022Quote: ... anti-H3K27me3 (Antibody 1: Active Motif, 39055; Antibody 2: Thermo Fisher, MA5-1198) or IgG (Epicypher ...