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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for Cytosolic arginine sensor for mTORC1 subunit 2 CASTOR2 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... anti-CD45RA-FITC (clone HI100, ThermoFisher Scientific), anti-IL-17A-PE (clone BL168 ...
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bioRxiv - Cell Biology 2024Quote: ... FITC NHS ester (#46410, Thermo Fisher Scientific), EZ-Link NHS-Biotin (#20217 ...
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bioRxiv - Immunology 2021Quote: Conditioned medium was obtained by culturing CECs in the arginine-free RPMI-medium (SILAC RPMI-medium, Thermo Fisher Scientific) supplemented with 150 µM ʟ-arginine at the density 1×106 cells/ml for 24h ...
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bioRxiv - Neuroscience 2020Quote: CGN WT and KO cultures (1.5-1.7×106 cells per culture) were grown in custom-made lysine and arginine free NB (Life technologies) to which “medium” (M ...
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bioRxiv - Molecular Biology 2024Quote: ... and LETIILSK were selected for synthesis (AQUA QuantProHeavy peptides with 13C15N-labeled C-terminal lysines or arginines, Thermo Scientific). The optimized LC-MS settings for these eight peptides can be found in Supp ...
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bioRxiv - Synthetic Biology 2024Quote: ... was incubated at 30°C for 8 h in a customized PURE system in which Lys and Arg were replaced with stable isotope amino acids (0.36 mM 13C6 L-lysine-2HCl, 0.36 mM 13C6, 15N4 L-arginine-HCl) (Thermo Scientific) to express aaRS ...
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bioRxiv - Cancer Biology 2023Quote: ... 90 µL of supernatant was mixed with 410 µL of IP Dilution Buffer and antibodies (Positive antibody: 10 µL; Negative antibody: 2 µL; FoxO3a antibody: 8 µL (Cat. #720128, Invitrogen)) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... cells were stained with anti-CD4-e450 (eBioscience) and anti-CD8-FITC antibody (eBioscience) followed by incubation with 100 µM mBcl (Monochlorobimane, Invitrogen) as described (Jayaraman and Jayaraman ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: We used murine FITC-conjugated anti-CD86 or murine PE-conjugated anti-MHCII antibodies from Thermo Fisher (Waltham, MA, USA) to stain the activation markers CD86 and MHCII ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were washed and stained for flow cytometry using appropriate seconday antibodies: goat anti-human fluorescein isothiocyanate (FITC; ThermoFisher A18812), anti-mouse Alexa Fluor 488 (Cell Signaling Technology 4408S) ...
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bioRxiv - Biochemistry 2022Quote: ... in PBS containing 1 mM CaCl2 and 1 mM MgCl2 were stained separately with either FITC-conjugated anti-CD18 antibody MEM48 (Thermofisher) at 1:100 dilution or APC-conjugated CBRM1/5 at 0.5 μg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with respective FITC- or Alexa Fluor 647-conjugated secondary antibody (Sigma-Aldrich F9137 or Thermo Scientific A21244) at a dilution of 1:100 for 1 h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... the oocytes were incubated with the FITC-conjugated goat anti-mouse IgG (H + L) secondary antibodies (1:300, Molecular Probes) for 1 h at 37°C ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T-cells were identified using a mouse-anti human CD4 (OKT4 clone) primary mAb and FITC goat anti-mouse secondary antibody (Invitrogen). The cells were then fixed using 4% paraformaldehyde before staining the nuclei with Hoescht 33342 (Invitrogen) ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were incubated for 1 h in the dark with a secondary antibody conjugated to fluorescein isothiocyanate (FITC; Invitrogen). Next ...
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bioRxiv - Immunology 2023Quote: ... and resuspended in 150 µL of an antibody cocktail containing 1:200 anti-Ter119 (FITC; Thermo Scientific, 11-5921-82), 1:50 anti-CD31 (FITC ...
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bioRxiv - Immunology 2023Quote: ... using permeabilization buffer (eBioscience) and stained with the following cytokine antibodies for 30 min at 4°C: IL-17A (eBio17B7, FITC, Invitrogen), IFN-ψ (XMG1.2 ...
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bioRxiv - Immunology 2024Quote: Isolated cell suspensions were stained for 30 minutes on ice with the following fluorescently conjugated antibodies: CD3-FITC (clone 17A2, Invitrogen), CD3-BV605 (17A2 ...
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bioRxiv - Immunology 2024Quote: ... The following anti-non-human primates or human antibodies were used: FITC- conjugated anti-CD3 (ThermoFisher Scientific Cat. No. APS0308), Alexa700-conjugated anti-CD4 (ThermoFisher Scientific Cat ...
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bioRxiv - Bioengineering 2024Quote: ... both sensors expressed in the cells with live cell imaging solution with 10mM glucose (LCIS+, Gibco, A14291DJ) were imaged using an epifluorescence microscope ...
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bioRxiv - Biochemistry 2023Quote: ... wild-type α-actin and 15 μM Pi-sensor (MDCC-labeled phosphate binding protein, Thermo Fisher Scientific) was prepared ...
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bioRxiv - Biochemistry 2024Quote: Single turnover RasGAP kinetics assays were performed using the Phosphate Sensor system62,63 to detect inorganic phosphate (Invitrogen; ThermoFisher Scientific catalogue number PV4407) ...
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bioRxiv - Microbiology 2021Quote: ... cells were fixed with 4% PFA for 30 minutes and stained for intracellular ENV with 1A1D-2 (1/500) monoclonal antibody and anti-mouse 647-conjugated antibody (2 μg/ml, Invitrogen Cat# A21235).
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bioRxiv - Developmental Biology 2022Quote: ... Nuclear and cytosolic fractions of iPSCs-differentiated cells were acquired using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific #78833) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... we introduced a stop codon in the pLRB302 plasmid at residue 640 of the envelope ORF (C640Stop) located at the N’ terminal of the cytosolic tail using the Phusion Site-Directed Mutagenesis Kit (Thermo Fisher Scientific) and the following primers ...
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bioRxiv - Immunology 2020Quote: ... and soluble cytosolic protein (SCP) fractions were extracted from Chinese hamster ovary (CHO) cells and biotinylated using NHS-LC-Biotin (Thermo Fisher Scientific) reagent ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations of both the cytosolic and membrane fractions were measured using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific) and equal amounts of protein were loaded on to a NuPAGE 4-12% Bis-Tris gel (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentrations in both the cytosolic and membrane-enriched fractions were estimated using the Micro BCA™ Protein Assay Kit (Cat no. 23235, Thermo Scientific). Equal amounts of protein samples were separated by SDS-PAGE ((4-20% ...
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bioRxiv - Bioengineering 2020Quote: ... the cells were fixed and stained with FITC-Mab (Hypoxyprobe) and FITC HRP MAb (Hypoxyprobe) and Hoechst (Thermo Fisher). The result of staining was then imaged using Nikon fluorescent microscope (Figure S4) ...
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bioRxiv - Cell Biology 2022Quote: ... resuspended in a complete culture medium containing 1 mg/ml FITC-dextran (70,000 MW FITC-dextran; Thermo Fisher Scientific), and incubated at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... then FITC signal was amplified using anti-FITC HRP and AlexaFluor 488 Tyramide Signal Amplification Kit (Thermo Fisher Scientific). Stained slides were mounted with ProLong Gold Antifade Mountant with DAPI (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... A second injection of 2 MDa FITC dextran (TdB Consultancy, Uppsala Sweden) or Qtracker™ 655 Vascular Label (Invitrogen, Carlsbad CA USA) was performed to observe the vasculature morphology and identify vascular tissue ...
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bioRxiv - Bioengineering 2022Quote: ... the medium reservoirs were aspirated and 30 µL EGM-2 containing 25 µg/mL 70 kDa FITC-dextran dye (Thermo Fisher Scientific) added to a single reservoir ...
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Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeabilitybioRxiv - Molecular Biology 2020Quote: ... and a mixture of 2000 kDa FITC-dextran and 70 kDa Texas Red-dextran (2 mg/ml in embryo water; Life Technologies, Inc.) was injected ...
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bioRxiv - Physiology 2024Quote: ... at an effector/target ratio of 2:1 in the presence of mouse anti-human CD107a-FITC (11-1079-42; Thermo Fisher Scientific). Cocultures between CTLs and EndoC-βH1 cells expressing HLA-A2 were performed at 37 °C for 1.5 h in Iscove’s Modified Dlbecco’s Medium (IMDM ...
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bioRxiv - Cell Biology 2024Quote: A dual system was developed to trace both 0,57 mg/mL FITC-Dextran and 2 μM of the long-chain fatty acid analogue BODIPY-C12 (558/568) (ThermoFisher, cat. #D3835) in one well ...
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bioRxiv - Immunology 2024Quote: ... Primary antibodies of anti-SARS/SARS-CoV-2 Nucleocapsid Monoclonal Antibody (Invitrogen, MA1-7404) is diluted in section staining buffer at 1:400 and sections are stained at 4°C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... HA Tag antibody for SaCas9 protein (2-2.2.14, Thermofisher), rabbit anti-GFP (A11122 ...
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bioRxiv - Immunology 2020Quote: ... ILT-2 (CD85J) blocking antibody (clone HP-F1, ThermoFisher), CD155 blocking antibody (clone D171 ...
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bioRxiv - Microbiology 2022Quote: ... or Mouse-anti-HA tag (2-2.2.14) antibody (Invitrogen) (for HA-tagged vRNP complex pulldown experiment ...
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bioRxiv - Neuroscience 2020Quote: ... All the 2° antibodies were purchased from Thermo Fisher Scientific made in donkey and used for 1:800 dilution in labeling ...
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bioRxiv - Microbiology 2021Quote: ... antibodies targeting the SARS-CoV-2 nucleoprotein (N) (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... and anti-CD28 antibody (2 µg/mL; Thermo Fisher) for T cell activation for 72 h at 37°C incubator ...
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bioRxiv - Molecular Biology 2024Quote: ... mouse HA-probe antibody (Thermofisher, 2-2.2.14, 1:2000), rabbit HA-probe antibody (CST ...
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bioRxiv - Developmental Biology 2023Quote: ... anti-Sox 2 antibody (rabbit polyclonal, 1:100, Invitrogen) and anti-BrdU antibody (mouse monoclonal ...
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bioRxiv - Genomics 2023Quote: ... 2 μl of anti-CGGBP1 antibody (PA5-57317; Invitrogen) was added and incubated overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... 100μg anti-CD32b antibody (Thermo Fisher, clone AT130-2) was injected intraperitoneally 1 hour before OVA challenge ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 2 μg CEBPB antibody (PA5-27244; Thermo Fisher Scientific), or 2 μg control normal Rabbit IgG antibody (antibody 2729 ...
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bioRxiv - Biophysics 2021Quote: ... and 10 μg/mL Cholera Toxin Subunit B (Recombinant) Alexa Fluor™ 555 Conjugate (CTSB, Fisher Scientific) to label GM1 as the acceptor ...
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bioRxiv - Neuroscience 2021Quote: ... and FAM™ labeled HCN subunit probe (HCN1: Mm00468832_m1; HCN2: Mm00468538_m; HCN3: Mm01212852_m; HCN4: Mm01176086_m1; Applied Biosystems). A 7900HT Fast Real-Time PCR System (Applied Biosystems ...