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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for Cytosolic arginine sensor for mTORC1 subunit 2 CASTOR2 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Appropriate goat secondary antibodies (1-2 µg/ml) from ThermoFisher Scientific and Jackson ImmunoResearch were used to immunodetect primary antibodies.
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bioRxiv - Cancer Biology 2024Quote: ... then incubated in secondary antibodies for 2 hours (ThermoFisher Scientific). Primary and secondary antibodies were diluted in 1X TBS with 0.3% Triton and 1% normal horse serum ...
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bioRxiv - Neuroscience 2021Quote: ... or 13C6 L-Lysine-2HCl (Lys-8) and 13C6 L-Arginine-Hcl (Arg-10) for the metabolic incorporation of ‘light’ and ‘heavy’ stable isotopes respectively (ThermoFisher Scientific). HEK293FT cells were grown in light or heavy SILAC media for at least 10 doublings ...
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bioRxiv - Synthetic Biology 2021Quote: ... were directly added to yield 50 μL PURE system reaction mixtures [28] containing 13C6 15N4 L-Arginine (R*) and 13C6 L-Lysine (K*) (Thermo Scientific) as a substitute for non-labeled L-Arginine and L-Lysine ...
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bioRxiv - Cell Biology 2022Quote: ... transferred in media containing either low arginine concentrations (1%) or fully reconstituted supplemented with 10% dialyzed FBS (Gibco cat. 26400-044) (50×106 cells/condition ...
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bioRxiv - Systems Biology 2020Quote: ... SILAC DMEM medium (Thermo #88364) lacking L-arginine and L-lysine was firstly supplemented with 10% dialyzed FBS (Thermo Fisher, # 26400044) and the same penicillin/streptomycin mix ...
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bioRxiv - Systems Biology 2020Quote: G9a KO and control Raw 264.7 cells were cultured in AACT/SILAC DMEM medium supplemented with regular lysine and arginine (K0R0) and 10% dialyzed fetal bovine serum (Thermo Fisher), 1% penicillin ...
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bioRxiv - Cell Biology 2020Quote: Stable HA-GLUT4-GFP-expressing 3T3-L1 preadipocytes were grown for 5 doublings and differentiated in Lysine (LYS) and Arginine (ARG)-deficient DMEM (89985; Thermo Scientific), supplemented with 10% dialyzed FBS ...
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bioRxiv - Immunology 2020Quote: ... and 105.8 mg/mL L-Arginine U-13C6,U-15N2 R10 (CKGAS, #CNLM-539-H-0.25)) prepared in RPMI SILAC media (Thermo Scientific, #88365) supplemented with 10% dialyzed FBS (HyClone ...
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bioRxiv - Biochemistry 2023Quote: ... The malonylated synthetic peptide TVDGPSGKmaLWR* containing a stable isotope-labeled arginine residue (R* with 13C6 and 15N4) was obtained from Thermo Fisher Scientific at >90% chemical purity and >99% isotopic purity ...
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bioRxiv - Cell Biology 2024Quote: ... deficient in both L-lysine and L-arginine was supplemented with 1% dialyzed FBS and heavy amino acids 13C615N2 L-Lysine-2HCl and 13C615N4 L-Arginine-HCl (Thermo Scientific) at concentrations of 0.499 mM and 0.699 mM ...
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bioRxiv - Cell Biology 2024Quote: ... deficient in both L-lysine and L-arginine was supplemented with B27 with insulin and heavy amino acids 13C615N2 L-Lysine-2HCl and 13C615N4 L-Arginine-HCl (Thermo Scientific) at concentrations 0.219 mM and 1.149 mM ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed three times with PBS on the next day and incubated with corresponding secondary antibodies goat anti-rabbit IgG (H+L) FITC (Thermo Fisher Scientific), goat anti-mouse IgM (H ...
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bioRxiv - Cancer Biology 2021Quote: ... samples were incubated with secondary antibodies (FITC anti-mouse, 1:1000, Jackson Immuno Research, #715-545-150; AlexaFluor564 anti-rabbit, 1:500, Thermo Fisher, #A11035) for 2hr at RT ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated with the primary antibody solution (1:100 dilution; PDGFRA-APC, Thermo Fisher, cat#17-1401-81; SCA1-FITC, Thermo Fisher, 11-5981-82 ...
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bioRxiv - Molecular Biology 2022Quote: For one sample of selected RGCs two dissected retinas were dissociated as previously described 58,11 and stained with CD90.2(Thy-1.2)-FITC antibody (Thermo Fisher REF#11-0902-81). Briefly ...
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bioRxiv - Microbiology 2023Quote: FITC and Alexa Fluor 647 labelling of purified proteins was done by using FluoroTag FITC Conjugation Kit (MilliporeSigma, FITC1-1KT) and Alexa Fluor™ 647 Antibody Labeling kit (Invitrogen, A20186), respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... The following day primary antibody was removed and washing was done with 1x TBS thrice at intervals of 5 minutes cells were incubated in appropriate concentration of FITC conjugated secondary antibody (Alexa Fluor 488 cat no. A11008 Invitrogen, Carlsbad, USA) prepared in 1% BSA in 1X TBS at dilution of 1:1000 was added at 37°C in the dark and incubated for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... The resulting cells were then stained by a FITC-conjugated anti-rabbit IgG secondary antibody (4300581, eBioscience) and analyzed on an Attune NxT Flow Cytometer (Thermo Fisher Scientific). The data were processed by Flojo software (FlowJo ...
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bioRxiv - Cancer Biology 2024Quote: ... After 30 minutes of incubation at room temperature cells were washed twice with PBS and FITC conjugated secondary goat anti-rabbit polyclonal antibodies (Thermofisher; Cat # 31635) were added ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 μg nuclear proteins or 7.5 μg cytosolic proteins were boiled in loading buffer with reducing reagents (Invitrogen, NuPAGE™ LDS Sample Buffer (4X), NP0007) ...
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bioRxiv - Cell Biology 2024Quote: Whole retina was fractionated into cytosolic and post-cytosolic (mitochondrial enriched) fractions as previously described (Weh et al., 2020) using the Subcellular Protein Fractionation Kit for Tissues (Thermo Fisher Scientific, Cat # 87790). Both retinas from a single animal were pooled and homogenized with a Dounce homogenizer in cytoplasmic extraction buffer supplemented with protease (HaltTM Protease Inhibitor Cocktail ...
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bioRxiv - Immunology 2021Quote: ... The spots on a High Capacity Amine Sensor chip (Bruker) were immobilized with streptavidin (ThermoFisher Scientific, Waltham, MA, USA) (50 µg/ml diluted in 10 mM sodium acetate buffer pH 5.0 ...
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bioRxiv - Cell Biology 2023Quote: ... We used Premo™ Autophagy Tandem Sensor RFP-GFP-LC3 Kit (P36239, Molecular Probes, Thermo Fisher Scientific, Bremen, Germany) that allows visualization of the maturation of the autophagosomes to the autolysosomes ...
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bioRxiv - Biochemistry 2024Quote: ... 50 μL reactions were prepared with 8 nM SWR1C mixed with 0.5 μM phosphate sensor (fluorophore-conjugated phosphate binding protein, ThermoFisher) in remodeling buffer ...
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bioRxiv - Neuroscience 2023Quote: Olfactory projection neurons were stained by backfilling with the calcium sensor dye Fura-dextran (potassium salt, 10,000 MW; Invitrogen). Briefly ...
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: The prototype sensor was designed in silico by sequence alignment (Clustal Omega2) and ordered as a geneblock (Thermo Fisher) flanked by HindIII and NotI restriction sites to be subsequently cloned into pCMV vector (Addgene #111053) ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies (Table 2) were diluted 1:100 in blocking solution and secondary antibodies (Life Technologies) were diluted 1:200 in blocking solution ...
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bioRxiv - Genetics 2022Quote: ... dead and live cells was determined by FITC Annexin V/Dead Cell Apoptosis Kit with FITC annexin V and PI for flow cytometry (Invitrogen cat. #V13242), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... FITC Annexin V/Dead Cell Apoptosis Kit (Invitrogen™) was used to detect apoptosis ...
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bioRxiv - Immunology 2021Quote: ... and anti-CD11b-FITC (#11-0112-85, Invitrogen, UK). For in vitro stimulation of SFs ...
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bioRxiv - Neuroscience 2020Quote: ... FITC and rhodamine dyes were purchased from Thermo Fisher Scientific and FlAsH-EDT2 from Cayman Chemicals (USA) ...
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bioRxiv - Cancer Biology 2021Quote: ... or 4 µg/mL FITC-conjugated UEA1 (Thermo Scientific) according to manufacturer’s protocol and following standard immunofluorescence protocols.
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bioRxiv - Cancer Biology 2021Quote: ... anti-H-2Db (FITC, 28-14-8, Thermo Fisher), and anti-PD-L1 (10F.9G2 ...
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bioRxiv - Cancer Biology 2022Quote: ... and CD54-FITC (#11-0541-82) from Thermo Fisher; CD49a-PE (#562115 ...
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bioRxiv - Cell Biology 2021Quote: ... and 20μg/ml FITC-Neutravidin (A2662, Thermo Fisher Scientific) for 30mins ...
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bioRxiv - Cell Biology 2021Quote: ... and anti-mouse Ig-FITC (1:100,ThermoFisher,USA). Coverslips were mounted using Vectashield-DAPI-mounting-medium (Vector Laboratories,USA) ...
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bioRxiv - Biochemistry 2021Quote: ... FITC+ cells were collected directly into TRIzol (Life Technologies) solution and immediately frozen in dry ice.
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bioRxiv - Cancer Biology 2021Quote: ... injection of FITC-conjugated dextran (MW20,000, Thermo Fisher Scientific) 20 min prior to sacrifice ...
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bioRxiv - Pathology 2021Quote: ... CD16-FITC (Clone: eBioCB16) from eBioscience (Thermo Fisher, Germany). Anti-CD45-Pacific Blue (Clone ...
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bioRxiv - Immunology 2020Quote: ... sections were then incubated with anti-rabbit FITC (Invitrogen) and streptavidin NL-557 (R&D systems ...
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bioRxiv - Immunology 2021Quote: ... A FITC Annexin V/Dead Cell Apoptosis Kit (Invitrogen), containing FITC conjugated Annexin V and PI ...
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bioRxiv - Neuroscience 2022Quote: ... anti-V5-FITC and anti-V5-HRP (Life Technologies), mouse anti-Myc-HRP (clone 9E10 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated with NeutrAvidin-FITC (ThermoFisher Scientific; 31006) diluted to 25 μg/mL in with 2.5% milk in 4x SSC with 0.1% Tween-20 for 10 min for 1 h at 37 °C in a dark ...
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bioRxiv - Genomics 2023Quote: ... Alexa Fluor 488-conjugated goat anti-FITC IgG (Invitrogen).
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bioRxiv - Microbiology 2023Quote: ... and signals were revealed using streptavidin-FITC (Invitrogen, USA). Trails associated with sporozoites were counted using a Nikon 80i fluorescence microscope.
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bioRxiv - Physiology 2023Quote: ... and donkey-anti rabbit IgG conjugated FITC (Thermo Fisher). Sections were mounted in Antifade Mounting Medium with 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cancer Biology 2024Quote: ... macropinocytosis was assessed by a FITC-Dextran (ThermoFisher Scientific) uptake assay ...
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bioRxiv - Genomics 2022Quote: ... the FITC AnnexinV/Dead Cell Apoptosis Kit (Invitrogen, V13242) was used ...
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bioRxiv - Molecular Biology 2022Quote: ... at 1μM and 2M MW Dextran-FITC (Invitrogen, MA) (ex 494nm/em 521nm ...