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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for 1 4 Nitrophenyl cyclobutanecarbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... enzymatic dissociation was performed for 4–6 minutes at 37°C in 1 mL TrypLE (Invitrogen), then cell pellets were washed with ice-cold PBS and lysed with 1 mL of TRIzol (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... We resuspended the tubulointerstitial single cells with 1 mL 4% neutral buffered formalin (Thermo Fisher Scientific) and fix for 20 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... psPAX2 and pMD2.G with a ratio of 4:3:1 in Opti-MEM (Gibco, 31985070) using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... pH 7.4) and incubated at 4 °C with primary antibodies (Mouse anti-Cx26, 1:100, Invitrogen; Rabbit anti-Cx26 ...
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bioRxiv - Bioengineering 2022Quote: ... and sulfosuccinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate (sulfo-SMCC) were purchased from Thermo Scientific (USA). Human BDNF ...
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bioRxiv - Cancer Biology 2022Quote: ... and 4′6-diamidino-2-phenylindole (DAPI, nuclei staining, 1:10000; Cat. no. D1306, Life Technologies) diluted in PBS for 30 min at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides then were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) or with YOYO-1 (ThermoFisher). When HPV probe signal co-localized with YOYO-1 signal detecting DNA at 63x magnification ...
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bioRxiv - Bioengineering 2022Quote: ... counterstained with 1 μg/mL of 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific, #D1306) for 30 minutes ...
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A stable reference human transcriptome and proteome as a standard for reproducible omics experimentsbioRxiv - Cell Biology 2022Quote: ... the C18 columns were condition with 1 mL acetonitrile (ACN) (Thermo Scientific™, A955-4, USA), then equilibrated with 1 mL 5% ACN with 0.5% trifluoroacetic acid (TFA ...
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bioRxiv - Cell Biology 2022Quote: ... samples were incubated 2hrs at 4°C with secondary antibody (1:1000 in blocking solution; Invitrogen). Following extensive washing ...
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bioRxiv - Cell Biology 2022Quote: ... 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Thermo Fisher Scientific, Cat. no. 15630-080,) and 1% penicillinstreptomycin (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... with 1 ml per 4 million cells of 3 mM disuccinimidyl glutarate (DSG) (ThermoFisher Scientific, 20593) for 40 min at room temperature and quenched by 0.4 M Glycine for 5 min ...
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bioRxiv - Microbiology 2022Quote: ... Samples were also spread on agar plates supplemented with 4 µg mL-1 rifampicin (Fisher Scientific).
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated overnight at 4°C with isolectin GS-IB4 (1:100; Invitrogen, Cat# I32450) in PBS with 1% Triton X-100 ...
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bioRxiv - Bioengineering 2022Quote: ... 0.1 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (pH = 8.5) (ThermoFisher Scientific, Waltham, MA), 4-benzoylbenzyl-trimethylammonium chloride (PLPP ...
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bioRxiv - Cell Biology 2022Quote: ... VOs we’re fixed for 1 hour in 4% paraformaldehyde solution (Thermo Fisher Scientific, 7732-18-5) in PBS and for 2 hours in 2.5% glutaraldehyde in PHEM buffer (TAAB ...
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bioRxiv - Neuroscience 2024Quote: ... the slides were incubated overnight at 4°C with primary antibodies (anti-GFAP 1:1000, Invitrogen #PA1-10004 ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies were incubated overnight at 4°C using TM4SF1 (ThermoFisher Scientific #PA5-21119, 1:200) and CD31 (Dako #M0823 ...
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bioRxiv - Microbiology 2023Quote: ... 3% sucrose) diluted 1:4 in Leibovitz’s L-15 medium without phenol red (Gibco, Waltham, MA) and an adjusted osmolality of 340 mOsm using 1 M sucrose ...
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bioRxiv - Immunology 2023Quote: ... Slides were washed and stained with 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, D3571, 1:4000) in PBS for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... The supernatant was pre-cleared for 1 hour at 4°C with protein A Dynabeads (Invitrogen). Chromatin samples were incubated overnight at a low temperature with 10 µg ChIP-grade anti-RNA polymerase II (ab26721 ...
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bioRxiv - Neuroscience 2023Quote: ... slices were fixed in 4% PFA and Alexa488-coupled streptavidin (1:10,000 in TBS, Molecular Probes) or Alexa647-coupled streptavidin (1:10,000 in TBS ...
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bioRxiv - Molecular Biology 2023Quote: ... enzymatic dissociation was performed for 4–6 min at 37 °C in 1 ml TrypLE (Invitrogen), then cell pellets were washed with ice-cold PBS and lysed with 1 ml of TRIzol (Invitrogen) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Sections were briefly fixed with 95% ethanol for 1 min followed by 4% paraformaldehyde (Thermo Scientific) for 15 min ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were incubated overnight at 4°C with 1:50 SMARCC1 primary antibody (Invitrogen PA5-30174). Samples were then incubated with 1:100 donkey anti-rabbit (Jackson ImmunoResearch 711-035-152 ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were run 1 cm into a 4-12% NuPAGE BisTris gradient gel (Life Technologies). The proteins were stained using colloidal Coomassie Brilliant Blue and the gel piece containing the proteins was cut out ...
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bioRxiv - Cell Biology 2023Quote: ... iPSCs (1 × 10^4/well) were dissociated into single cells with accutase (Thermo Scientific, MA, USA) after exposure to the rock inhibitor (Y-27632 ...
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bioRxiv - Synthetic Biology 2023Quote: ... before adding the HRP conjugated secondary antibody 1:10000 in 4% milk in PBS-T (Invitrogen) for 1 hour ...
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bioRxiv - Immunology 2023Quote: AML and MDM cells were fixed with 4% formaldehyde and 1% glutaraldehyde in phosphate buffer (Invitrogen) overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... or Caspase-1 over-night 4°C and with fluorochrome-conjugated secondary antibody (Thermo Fisher Scientific) for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... cells were blocked in 1% BSA/0.1% saponin-PBS with 4% horse serum (26050-070, Gibco) for 1h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation overnight at 4°C with secondary antibodies (1:1000, Alexa-Fluor conjugated, Invitrogen). Following incubation in secondary antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... at 1% B with Solvent A as 0.1% formic acid in water (Thermofisher Optima LS118-4) and Solvent B as 0.1% formic acid in acetonitrile (Thermofisher Optima LS120-4) ...
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bioRxiv - Neuroscience 2023Quote: ... for 72 h at 4°C and secondary antibody anti-mouse Alexa-488 (1:300, Invitrogen).
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bioRxiv - Cancer Biology 2024Quote: ... An α-Mouse IgG2a-AF488 secondary antibody (Invitrogen, CAT#: A-21131; 1-4 hours, 25°C), cells were washed 3 times ...
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bioRxiv - Immunology 2024Quote: ... These were transfected at a 1:2:4 w/w ratio using Lipofectamine 3000 (Invitrogen #L3000001), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and then 1 h at 4°C at 7-10 rpm with dynabeads protein A (Invitrogen), equilibrated with the EB ...
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bioRxiv - Immunology 2024Quote: ... Th1 consisted of 1 µg/mL anti-murine IL-4 (Cat# 16-7041 Invitrogen Carlsbad, CA), 5 ng/mL murine IL-2 (Cat# 212-12 Peprotech Cranbury ...
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bioRxiv - Synthetic Biology 2024Quote: ... The supernatant was incubated at 4 °C for 1 h with HisPur Ni-NTA resin (ThermoFisher), after which the samples were applied twice to a gravity flow column at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... mouse anti-E-cadherin (1:500, overnight at 4°C, catalog no. 14-3249-82; Invitrogen), goat anti-rabbit conjugated with horseradish peroxidase (HRP ...
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bioRxiv - Molecular Biology 2024Quote: ... 4 µL of Maxima RT buffer and 1 µL of Maxima reverse transcriptase (Life Technologies, #EP0751). The samples were mixed by pipetting and incubated for 30 min at 60 °C ...
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Placentas from SARS-CoV-2 infection during pregnancy exhibit foci of oxidative stress and DNA damagebioRxiv - Microbiology 2024Quote: ... primary antibodies overnight at 4℃ followed by anti-rabbit IRDye 800CW secondary antibody (1:3000, Invitrogen) for one hour at room temperature ...
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bioRxiv - Immunology 2024Quote: ... at a 4:1 mole ratio in the presence of T4 DNA ligase (Thermo Scientific, USA). Ligation products were concentrated by isopropanol precipitation and separately transformed into 25 µl electrocompetent Escherichia coli (E.coli)TG1 cells (BioCat ...
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bioRxiv - Genomics 2024Quote: ... and lacking sodium pyruvate and HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (McCoy‘s 5A, Gibco 16600082), supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Genetics 2024Quote: ... with 1 ml per 4 million cells of 3 mM disuccinimidyl glutarate (DSG) (ThermoFisher Scientific, 20593) for 40 min at room temperature and quenched by 0.4 M Glycine for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... for 1 hat 150 V at 4 °C before switching to light cathode buffer (Invitrogen, BN2002) to run overnight at 20-30 V at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... OFC sections were reacted with the primary antibody for rabbit met-enkephalin (1:400, Abeam ab22620) for 24h at 4°C and Alexa-fluor 488 goat anti rabbit secondary antibody (Invitrogen, 1:400) for 24 hours at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... the samples were washed with PBS and incubated in the dark for 1 h at 4 °C with secondary antibody (Alexa Fluor-568-conjugated anti-mouse antibody, 1:750, Thermo Fisher Scientific). Finally ...
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bioRxiv - Molecular Biology 2022Quote: ... at 30 V for 1 hour followed by100 V for a further hour at 4°C in 1× NuPAGE Transfer Buffer (Thermo Fisher Scientific) supplemented with 0.1 g/l of SDS (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... (alone or in combination) in IF washing buffer overnight at 4°C: anti-ZO-1 rabbit polyclonal (1:200) (Thermo Fisher Scientific), anti-E-cadherin rabbit monoclonal (1:200 ...