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Citations for Thermo Fisher :
801 - 850 of 10000+ citations for 1 4 Nitrophenyl cyclobutanecarbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... aeruginosa antibody65 (1 : 500 dilution) at 4°C overnight and secondary antibody (Alexa Fluor™ 647 chicken anti-rabbit IgG, Invitrogen; 1 : 500 dilution) at room temperature for 1h ...
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bioRxiv - Cell Biology 2022Quote: ... 1:200 O/N at 4 °C) and Alexa Fluor 594-conjugated anti-mouse IgG antibody (#A11005, Invitrogen, 1:2000 for 2 hours RT). Nuclei staining was done in 0.5 μg/mL Hoechst 33324 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... Brain sections were then incubated overnight at 4 °C in rabbit anti-MYOC (1:50, custom) and rat anti-GFAP (Life Technologies, 130300, 1:500) in 5% NGS (MP Biomedicals ...
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bioRxiv - Microbiology 2023Quote: ... and primary antibody staining was carried out overnight at 4°C (polyclonal anti-PBP2a, custom antibody from Thermo Fisher, 1:1000 or polyclonal anti-Sortase A, custom antibody from Thermo Fisher 1:1000). Secondary antibody staining was performed using an anti-rabbit antibody (Azure anti-rabbit NIR700 or Azure anti-rabbit NIR800 at 1:20000 dilution) ...
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Single-nucleus multi-omics identifies shared and distinct pathways in Pick’s and Alzheimer’s diseasebioRxiv - Neuroscience 2024Quote: ... at 4°C overnight (IBA1 antibody; Cat #NC9288364; 1:1000; Fisher Scientific, GFAP Polyclonal Antibody; Cat #PA3-16727; 1:500; ThermoFisher, p-tau (AT8) Cat #MN1020 ...
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bioRxiv - Cell Biology 2022Quote: ... with an Eagle 4 K x 4 K CCD camera system (Ceta CMOS, ThermoFisher).
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bioRxiv - Molecular Biology 2020Quote: ... 4 µg protein was resolved on NuPAGE 4-12% Bis-Tris protein gels (ThermoFisher) and transferred to PVDF membrane (Fisher) ...
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bioRxiv - Neuroscience 2022Quote: ... containing 2 μM cell-permeant Fluo-4 acetoxymethyl ester (Fluo-4-AM, Thermo Fisher) with the addition of the equal quantity (1:1 v/v ...
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bioRxiv - Immunology 2022Quote: ... Hemocytes were fixed in 4% PFA (4% in PBS; J19943-K2, Thermo Fisher Scientific) for 30 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 4% formaldehyde (4% FA, 10% formalin [Thermofisher Scientific, #119690010] in 1x PBS), dissected out the spinal column ...
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bioRxiv - Genetics 2021Quote: ... The cells were stained overnight at 4 °C with a 1:50 dilution of phalloidin-Alexa 546 (Invitrogen) made in blocking buffer followed by three washes each of 5 min ...
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bioRxiv - Cell Biology 2022Quote: ... Ovaries were washed 4×20min in PBT and then incubated in PBT containing DAPI (1:1000, Molecular Probes), with or without DiOC6 (1:5000 ...
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bioRxiv - Molecular Biology 2020Quote: ... Boiled samples were loaded into 4-12% 1-mm 12-well Bis-Tris NuPage gels (Invitrogen Cat#NP0322PK2) along with 0.5-μL of Benchmark Unstained Protein Ladder (Invitrogen Cat#10747-012 ...
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bioRxiv - Genomics 2020Quote: ... were washed in IP-buffer and incubated with 4 μg anti-AP-1 (Thermo Scientific™ #MA5-15172)or anti-Sp1 Ab (ChIPAb+™ Merck #17-601 ...
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bioRxiv - Synthetic Biology 2021Quote: ... for 0.5–1 hr at 4°C while rotating in a 10-mL Pierce disposable column (Thermo Scientific), and was washed and eluted using His60 or HisTalon Buffer Set (Takara Bio ...
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bioRxiv - Neuroscience 2021Quote: ... buffered with 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Life Technology, Thermo Fisher Scientific Inc., USA) and coated with 20 μg/mL laminin (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed 4 × 5 min with PBS before incubating with secondary antibodies (1:1000 Alexa fluorophores, ThermoFisher) in blocking solution for 1 hr at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were incubated overnight at 4°C with primary antibodies anti-ACE2 (1: 1000; MA5-32307 - Thermo Fisher), anti-CB1 (1:300 ...
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bioRxiv - Plant Biology 2021Quote: ... and incubated for 1 h at 4°C with 40 μL of Protein AG UltraLink Resin (Thermo Fisher). The beads were washed 2 × 5 min in ChIP Wash Buffer 1 (0.1% SDS ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mL 50% erythrocytes were transferred to 4 mL glass autosampler tubes (Thermo Fisher Scientific, 03-391-19), closed with screw caps with septa (Thermo Fisher Scientific 03-391-21) ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated overnight in mouse anti-Hu primary antibody at 4°C (1:200 in 3% block; Invitrogen). After three 5-min PB rinses at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... for 1 h with gentle rotation at 4°C and then protein-G coated magnetic beads (Thermo Fisher) were added and incubated for an additional 30 min ...
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bioRxiv - Microbiology 2021Quote: ... L454W/E455G and S262R) and mCherry (6:4:1 mixtures; 2.2 μg/well) using Lipofectamine® 2000 (Invitrogen), according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... 1 µg of sample was loaded on 4–12% NuPAGE Novex Bis-Tris polyacrylamide gels (Thermo Scientific, NP0322BOX) and ran for 7 min at 80V to stack proteins in a single band ...
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bioRxiv - Molecular Biology 2022Quote: ... The collected tissue was twice washed with 4 mL ice-cold Ca2+/Mg2+-free 1 × HBSS solution (Gibco) and submerged in 6 mL ice-cold lysis buffer (20 mM Hepes-KOH ...
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bioRxiv - Neuroscience 2020Quote: ... 4) counterstained with the nuclear dye TO-PRO-3 (diluted 1:10’000; Life Technologies, Inc., Gaithersburg, MD, USA). Finally ...
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In vivo imaging of the kinetics of microglial self-renewal and maturation in the adult visual cortexbioRxiv - Neuroscience 2020Quote: ... clone G-A-5) followed by a secondary antibody solution (4 h, RT, AlexaFluor 594, 1:500, Invitrogen), mounted and coverslipped ...
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bioRxiv - Microbiology 2021Quote: ... 4 Transwell inserts of HAE-ALI cultures were dissolved in 1 ml of TRIzol Reagent (ThermoFisher, Waltham, MA), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The primary antibody was incubated overnight at 4°C [human Pax6 polyclonal antibody (1:100, Thermo Fisher Scientific)] ...
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bioRxiv - Cell Biology 2022Quote: ... nerves were cut and subsequently incubated for 10 min in HL3 with 4 µM FM 1-43 (Invitrogen), 1.5 mM CaCl2 and 90 mM KCl ...
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bioRxiv - Immunology 2020Quote: ... 4 million cells/mL in RPMI++ were loaded with 1.5 μM of the calcium indicator Indo-1 (Invitrogen) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... GMPCPP-stabilized MT seeds were prepared by polymerizing a 1:4 molar ratio of tetramethylrhodamine (TAMRA, ThermoFisher Scientific) labeled:unlabeled tubulin (Hyman ...
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bioRxiv - Immunology 2020Quote: ... Cells were then stained with 1 μg/mL 4′,6-diamidino-2-phenylindole (DAPI; Molecular Probes/Thermo Fisher) and analysed on a FACSVERSE (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... Cells were then stained with 1 μg/mL 4′,6-diamidino-2-phenylindole (DAPI; Molecular Probes/Thermo Fisher) and analysed on a FACSVERSE (BD Biosciences) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed once with Krebs-Ringer’s buffer containing 1.8 mM calcium chloride and loaded with Fluo-4 AM (1 μg/μl; ThermoFisher) plus 10 μl 0.2 % pluronic acid for 30 min at 37 °C ...
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bioRxiv - Physiology 2020Quote: ... DAPI (4’,6-Diamidino-2-Phenylindole Dihydrochloride; 1:2000 diluted in PBTD; Thermo Fisher Scientific, Stockholm, Sweden; 62248) was added for 3 minutes followed by washing in PBTD for 25 minutes and mounting with ProLong gold antifade reagent (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... and incubated for 1 h at 4°C with 40 µL of Protein AG UltraLink Resin (Thermo Scientific). The beads were washed 2 × 5 min in ChIP Wash Buffer 1 (0.1% SDS ...
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bioRxiv - Neuroscience 2021Quote: ... slices were counterstained with 4’,6-diamidine-2’-phenylindole 502 dihydrochloride (DAPI, D3571, Molecular Probes; dilution 1:1000) for 5 minutes followed by 2 minutes wash in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Paris) and pCMV-VSV-G at a ratio of 4:3:1 with Lipofectamine 3,000 (Thermo Fisher Scientific). Supernatants were collected 48 h after transfection ...
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bioRxiv - Cancer Biology 2020Quote: All cells were sub-cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, (25 mM Glucose, 4 mM Glutamine, 1 mM Sodium Pyruvate) (GIBCO)) supplemented with 10% fetal bovine serum and 50 units/mL Penicillin/Streptomycin (DMEM++ ...
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bioRxiv - Immunology 2022Quote: ... stimulator PBMCs were irradiated at 35 Gy and cultured (ratio 4:1) with CFSE-labeled responder PBMCs (Invitrogen, Waltham ...
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bioRxiv - Cell Biology 2022Quote: ... Nuclei were counterstained using 4′,6-diamidin-2-phenylindol (DAPI, 1:1000, 7 min; D1306, Thermo Fisher Scientific). Washing steps were performed with PBS ...
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bioRxiv - Bioengineering 2022Quote: ... the cell nuclei were stained with 1 μg/mL 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific #62248) in DPBS for 15 min at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... and pGL4.74-Renilla luciferase plasmids in a ratio of 0.5:1:0.1 (4 μg/6-well dishes) using Lipofectamine LTX PLUS (Invitrogen). The luciferase activity was assayed using the Dual-Luciferase Reporter Assay Kit (Promega) ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were either washed using DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride; 1:10000; Thermo Fisher Scientific, MA) made up in di H2O to stain for nuclei before being mounted or cover-slipped with ProLong Gold mounting medium with DAPI (Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated at 4°C for three nights with rabbit-anti-PDGFRβ (1:100; Thermofisher, Cat# ab32570), guinea pig-anti-IBA1 (1:500 ...
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bioRxiv - Neuroscience 2022Quote: Asteroids from each condition (1 to 4 asteroids per well) were embedded within Geltrex (Thermo Fisher cat# A1413301) in a 48-well plate to ensure stability during electrophysiological recording ...
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bioRxiv - Physiology 2022Quote: ... The samples were combined with 1 ml of 3:13 dilution of Erlich’s reagent [1.5 g of 4- (dimethylamino) benzaldehyde (ThermoFisher); 5 ml ethanol ...
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bioRxiv - Developmental Biology 2022Quote: ... centrifuged at 500xg for 5 min at 4°C and resuspended in 1% BSA in PBS (AM2616, Invitrogen) containing 200 U/μl RNase inhibitor (3335402001 ...
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bioRxiv - Cell Biology 2024Quote: ... F-actin was stained with AF-488 phalloidin (1 drop in 4 ml FACS buffer; Invitrogen, Cat. # R37110) for 30 minutes ...