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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for 1 4 Nitrophenyl cyclobutanecarbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... at 1:250 dilution overnight at 4°C and anti-mouse AlexaFluor-555 secondary antibody (1:1000, ThermoFisher Scientific, #A-21424) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Frozen rat ovarian follicles were homogenized in 400 µL lysis buffer (100 mM HEPES, pH 8, 4% SDS, 1 mM EDTA, and 1× Halt protease/phosphatase inhibitor cocktail (ThermoFisher Scientific) using Zirconia/glass beads in a bead beating grinder (FastPrep24 ...
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bioRxiv - Cell Biology 2024Quote: ... 1:600) antibody was incubated at 4°C overnight followed by secondary antibody anti-rabbit Alexa 488 (ThermoFisher Scientific, 1:600) for 1h at RT ...
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bioRxiv - Developmental Biology 2024Quote: ... and incubated at 4°C for 18 hours with primary antibodies: E-Cad (13-1700, Thermo Fisher, HECD-1, 1:100) and CK-14 (RB-9020 ...
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bioRxiv - Genomics 2023Quote: ... plugs were washed 4 times in 1×Wash Buffer (BNG) and 5 times in 1× TE Buffer (ThermoFisher Scientific, Waltham, MA). Then ...
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bioRxiv - Neuroscience 2023Quote: ... 4) with secondary Abs anti-mouse or rabbit IgG conjugated Alexa 488 (1:400) or Alexa 568 (1:200) (Life Technologies).
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bioRxiv - Immunology 2023Quote: NK cells were activated by co-culturing NK cells with irradiated K562 cells expressing membrane bound interleukin-21 and 4-1BBL at 1:1 ratio in RPMI (Gibco, Thermo Fisher) supplemented with 10% FCS (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... cDNA was diluted 1:3 or 1:4 before mixing with Power SYBR Green PCR Master Mix (CAS: 4368702, Applied Biosystems) and primer pairs (Table S4B) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Lot 134874), and 4-(2-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, purity ≥ 99.%, CAT #BP310-1, Lot 052975) were from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cell Biology 2022Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes). Cells were imaged using a DeltaVision Ultra microscope with a 60X objective (NA = 1.42) ...
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bioRxiv - Molecular Biology 2023Quote: ... or anti-NIPBL rabbit antibodies followed by 1 h incubation at 4 °C on a rotating wheel with 40 μl of a 1:1 mix of Dynabead protein A (10001D, Life Technologies) and G (10003D ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were washed and suspended with 1 mL of 4% FBS/PBS containing DAPI (1:2000; Thermo Fisher Scientific, 62248) and DRAQ5 (1:5000 ...
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bioRxiv - Biochemistry 2023Quote: ... containing 1 mM EDTA and 1 mM dithiothreitol were reacted with a 4-fold concentration of Protein Thermal Shift Dye (Life Technologies) in a 20 μl reaction volume ...
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bioRxiv - Cancer Biology 2024Quote: ... these plasmids were transfected into HEK293T cells alongside psPAX2 and pMD2.G at a mass ratio of 1 pMD2.G : 3 psPAX2 : 4 pLKO.1 using Lipofectamine 2000 (Invitrogen 11668019), and media containing the lentivirus was concentrated with Lenti-X Concentrator (Takara Bio 631232 ...
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bioRxiv - Neuroscience 2024Quote: ... and probed with the primary antibody overnight at 4°C (anti-Pyk2 [1:500; sc-1514, Santa Cruz Biotechnology] and anti-pPyk2 [1:500; 44-618G, Invitrogen]). After several washes with TBS-T ...
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bioRxiv - Cell Biology 2024Quote: ... All organoid media were refreshed every 3-4 days and organoids were passaged 1:4-1:8 every week by either mechanical disruption or enzymatic digestion using TrypLE Express Enzyme (Gibco, 12604013). If organoids were passaged by enzymatic digestion into single cells ...
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Malaria parasites undergo a rapid and extensive metamorphosis after invasion of the host erythrocytebioRxiv - Microbiology 2024Quote: Wildtype parasites and parasites lacking PV6 were allowed to invade erythrocytes for 4 hours in cRPMI and then washed and resuspended at 1% haematocrit in PBS containing 1% Albumax (Life Technologies). The retention rate was evaluated by microsphiltration (microsphere filtration ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed once with PBS and a mixture of 1:1:2 (w/w) of pcDNA5-αSyn (4 μg): pOG44 Flp-recombinase expression vector (pOG44, Invitrogen #V600520):PEI in 2 mL of DMEM was added for 8 h ...
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bioRxiv - Biophysics 2024Quote: ... protein fractions were mixed 1: 1 with SDS loading buffer and loaded on a NuPageTM 4-12% Bis-Tris PAGE Gel (Invitrogen™) along with Novex™ Sharp Pre-stained Protein Standards (range from 3.5 to 260 kDa ...
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bioRxiv - Neuroscience 2024Quote: ... and then washed in 0.1M PBS containing 0.1% tween before a 4 hr incubation in a half block solution containing 1:1000 goat anti-rabbit 488 (Invitrogen, A11008). Finally ...
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bioRxiv - Biochemistry 2024Quote: ... Following centrifugation at 48,000 rcf for 1 hour at 4°C the supernatant was applied to 1 mL Ni-NTA agarose beads (ThermoFisher Scientific) and rocked for 1 hour at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... A total of 4 μg of protein were extracted from each lysate and reduced with 1:1 dilution of 2-mercaptoethanol (Thermo Scientific) and 2× Laemilli Buffer (Biorad) ...
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bioRxiv - Cell Biology 2024Quote: Ovaries from 8 to 15 females were dissected in PBSTw (PBS with 0.1% Tween-20) and fixed in a 1:1 mixture of 4% formaldehyde (Thermo Scientific, 28908) in PBS and heptane (Sigma Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... 4 μl of 4 mM resazurin sodium salt (Acros Organics, Belgium), 0.002 U purified Castellaniella defragrans geraniol dehydrogenase ...
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bioRxiv - Neuroscience 2024Quote: The DIV 13-14 cells growing on coverslips were fixed using 4 % PFA freshly prepared from a 16 % stock (Thermo Fisher Scientific, dissolved 1:4 in 1xPBS) for 10 min RT ...
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bioRxiv - Physiology 2020Quote: ... muscles were stained for 3 minutes with 10μM 4-(4-diethylaminostyrl)-N-methylpyridinium iodide (4-Di-2ASP, Molecular Probes) to allow imaging muscle with an upright epifluorescence microscope (Leica DMR ...
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bioRxiv - Physiology 2021Quote: ... were immunostained for insulin (guinea pig anti-insulin pig from Abcam, 1:1000, overnight, 4’C, secondary antibody; Alexa Fluor 647-conjugated anti-guinea pig from Invitrogen; 1:500, 1 h room temperature) and nuclei were stained with DAPI (Sigma-Aldrich ...
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bioRxiv - Genetics 2024Quote: ... were incubated overnight at 4°C followed by the secondary antibodies (anti-rabbit 488, A11034 Invitrogen, 1:400; anti-mouse 555, A31570, Invitrogen, 1:400; DAPI, Roth, 1:400). Finally ...
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bioRxiv - Cell Biology 2020Quote: ... Infected MEFs were maintained in induction medium composed of DMEM/High Glucose:Medium 199 (4:1, HyClone:Gibco), 10% FBS (Gemini) ...
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bioRxiv - Biophysics 2020Quote: KPL-4 cells were transfected with the GV gene constructs and 1 µg/ml puromycin (Invitrogen) was added to cell cultures to select only the transfected cells ...
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bioRxiv - Neuroscience 2021Quote: ... slices were incubated with 4’,6-diaminodino-2-phenylindole (DAPI, Life Technologies D-21490, 1:2000) for 15 min ...
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bioRxiv - Neuroscience 2020Quote: ... The nuclear dye 4’,6-diamidino-2-phenylindole (DAPI) at 1 l ng/ml (Molecular Probes) was added to visualise all cells ...
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bioRxiv - Physiology 2020Quote: ... Cell nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI; 1:10,000; ThermoFisher, Table S13). Confocal images were acquired using an inverted laser scanning confocal microscope (Nikon Eclipse C1) ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were generally run in 1 mm polyacrylamide 4-20 % Novex MES/SDS gels (Thermo Scientific) and using a Mini Gel Tank ...
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bioRxiv - Immunology 2021Quote: Freshly isolated thymocytes or virus-transduced thymocytes were incubated with 4 μM Indo-1-AM (Invitrogen) in TCM at 37°C for 40 min ...
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bioRxiv - Immunology 2021Quote: ... Nuclei were counterstained with 1/2500 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, ThermoFisher Scientific, USA). The cover slips were fixed in fluorescence mounting medium (Fluoromount-G ...
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bioRxiv - Cancer Biology 2021Quote: ... Chromatin immunocomplexes were precipitated for 1 hour at 4°C with blocked Dynabeads Protein G (Invitrogen), subjected to multiple wash steps and the chromatin eluted in elution buffer (50 mM Tris-HCl [pH 8.0] ...
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bioRxiv - Immunology 2021Quote: ... Nuclei were counterstained with 1/2500 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, ThermoFisher Scientific, USA). The cover slips were fixed in fluorescence mounting medium (Fluoromount-G ...
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bioRxiv - Cancer Biology 2021Quote: ... with a ratio of 1 μg DNA and 4 μl transfection reagent diluted in OptiMEM (Gibco). Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... and 1 mM of the serine protease inhibitor 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (ThermoFisher Scientific). The samples were further ruptured using a syringe and centrifuged at 14,000g for 30 min at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... and 1 mM of the serine protease inhibitor 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (ThermoFisher Scientific). The samples were sonicated and centrifuged at 14,000g for 30 min at 4°C ...
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In vivo imaging of the kinetics of microglial self-renewal and maturation in the adult visual cortexbioRxiv - Neuroscience 2020Quote: ... Wako 019-19741) followed by secondary antibody solution (4 h, RT, AlexaFluor 488, 1:500, Invitrogen), mounted and coverslipped ...
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bioRxiv - Neuroscience 2021Quote: ... cells were loaded with 1 µM calcium-sensitive fluorescent dye (Fluo-4 AM; Invitrogen, Life Technologies) diluted in fresh Cl- buffer (154.6 mM ...
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bioRxiv - Neuroscience 2021Quote: ... cells were loaded with 1 µM calcium-sensitive fluorescent dye (Fluo-4 AM; Invitrogen, Life Technologies) diluted in fresh Cl- buffer (154.6 mM ...
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bioRxiv - Neuroscience 2022Quote: ... Remaining supernatant was incubated at 4°C for 1 hour with Sepharose-G beads (Fisher Scientific), previously washed with 5% BSA in TBS-Triton buffer and preincubated overnight with 2.5 μg anti-GFP antibody ab290 (Abcam) ...
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bioRxiv - Cell Biology 2020Quote: ... cultured for 4 days and directly stained with Hoechst 33342 (1 mg/mL) (Thermofisher Scientific, 62249) for 30 minutes at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Chamberslides were then incubated in 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI) (1:5,000, ThermoFisher Scientific) in 1x PBS for 5 min ...
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bioRxiv - Neuroscience 2020Quote: ... Free-floating sections were incubated overnight (4 °C) with rabbit anti-GFP (1:1000; Invitrogen, A11122), mouse anti-S100β (1:500 ...
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bioRxiv - Immunology 2021Quote: ... at cell yield from 1 brain per 4 transwell inserts pre-coated with 0.1% gelatin (Gibco), or in 12-well culture plates and monitored for confluence and polarisation ...
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bioRxiv - Immunology 2020Quote: ... cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen, Karlsruhe, Germany, 1:100) for 30 min at RT ...