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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for 1 4 Nitrophenyl cyclobutanecarbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... 4 μl 10 μM dNTP mix and 1 μl RNase inhibitor (40 U/μl, Ambion, Cat# AM2682) was added to each tube containing RNAclean XP beads (input group ...
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bioRxiv - Biochemistry 2022Quote: ... and maintained in Low Glucose DMEM (5.5 mM glucose, 1 mM pyruvate, 4 mM glutamine; #31600034, Gibco) supplemented with 10% fetal bovine serum (#12605729 ...
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bioRxiv - Biophysics 2022Quote: ... pH 8.0 supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) and 4 Pierce protease inhibitor tablets (Thermo scientific), then stirred gently on a Variomag magnetic stirrer MONO DIRECT (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: THP-1 cells were differentiated into 4-well Nunc Lab-Tek II Chamber slides (ThermoFisher, Waltham, MA) at a density of 105 cells per well and differentiated as described above ...
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bioRxiv - Microbiology 2022Quote: Spn serotype 4 strain TIGR4 was grown overnight on 1% agar- Todd Hewitt Broth (Thermo Fisher Scientific) with yeast (THY ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were counterstained with 4’,6-diamino-2-phenylindole dihydrochloride (DAPI, 1:10000 in PBS, Thermo Scientific) and mounted with a cover glass using Fluoromount (Diagnostic BioSystems ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclei were visualized with Hoechst (4′,6-diamidino-2-phenylindole) (DAPI) (Invitrogen; 3258, ICC/IHC 1:5,000).
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bioRxiv - Physiology 2022Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole (1:2500, DAPI, ThermoFisher Scientific, Cat No. D1306). Images were taken using Zeiss 880 confocal microscopy and quantified by using Fiji/ImageJ86.
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bioRxiv - Developmental Biology 2024Quote: ... The samples were electroporated in the MES buffer in 1 mm 4%-12% Bis-Tris gels (Invitrogen) and blotted onto PVDF membrane using the Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... and incubated with primary antibodies overnight at 4 °C: pSTAT1-Tyr701 (ThermoFisher Scientific MA515071, 1:1000 dilution), STAT1 (Santa Cruz Biotechnology SC-417 ...
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bioRxiv - Developmental Biology 2024Quote: ... Antibodies were used together with DAPI (4’,6-diamidino-2-phenylindole, Thermo Scientific, 62248, 1:1,000 dilution). NDE1 (Abnova ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were probed with an SLC35A2 polyclonal antibody overnight at 4°C (1:2000, Invitrogen, PA5-77178), followed by incubation with goat anti-rabbit HRP-linked secondary antibody (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... cell nuclei were stained with DAPI (4’,6-diamidino-2-phenylindole, Thermo Fisher Scientific, 1:5000 dilution) for 15 mins ...
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bioRxiv - Biochemistry 2023Quote: ... The slides were then incubated with 1 ug/ml of 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen) in 1xPBS for 20 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then stained using primary antibodies overnight at 4°C: tubulin (Invitrogen MA1-80017, 1:1000), aSyn (BD Transduction Laboratories 610786 ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclear stain: 4′,6′-diamidino-2-phenylindole dihydrochloride (DAPI, blue; 2 ng ml−1; Molecular Probes, USA). Sections were cover-slipped using ProLong Gold anti-fade reagent (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated at 4°C for 30 min with antibodies against CD45 (Invitrogen, #MCD4528, 1/25), CD31 (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... immortalized FSHD myoblasts were cultured in LHCN medium (4:1 DMEM:Medium 199 (ThermoFisher Scientific, Waltham, MA, USA) supplemented with 15% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2022Quote: ... pH 8.0 supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) and 4 Pierce protease inhibitor tablets (Thermo scientific), then stirred gently on a Variomag magnetic stirrer MONO DIRECT (Thermo Scientific ...
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bioRxiv - Genomics 2022Quote: ... 4 μl of 1 M CaCl2 and 5 μl of 100 U MNase (88216, Thermo Fisher Scientific) were added ...
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bioRxiv - Genetics 2023Quote: ... 1/10 of each eluate was resolved on a 4–12% NuPage Novex gel (Thermo Fisher Scientific) and stained with SilverQuest staining kit (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... for 1 hour at 4°C and then was loaded onto Pierce™ Glutathione Agarose (16101; Thermofisher) by gravity flow ...
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bioRxiv - Genomics 2023Quote: ... TaqMan qPCR reactions contained: 4× TaqPath 1-Step RT-qPCR multiplex reaction master mix (ThermoFisher, Cat# A28521), 4pmol of each primer ...
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bioRxiv - Molecular Biology 2023Quote: ... washed (4 x 300 µL PBST) and blocked for 1h at RT with 1% BSA (Fisher Scientific) in PBS solution ...
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bioRxiv - Bioengineering 2023Quote: ... constructs were fixed with 4% paraformaldehyde and washed with PBS and stained for LipidTox (Invitrogen 1:500) and DAPI (ThermoFisher ...
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bioRxiv - Genomics 2023Quote: ... TaqMan qPCR reactions contained: 4× TaqPath 1-Step RT-qPCR multiplex reaction master mix (ThermoFisher, Cat# A28521), 4pmol of each primer ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 hour at 4°C and then was loaded onto Pierce™ Glutathione Agarose (16101; Thermofisher) by gravity flow ...
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bioRxiv - Cancer Biology 2024Quote: ... Glut-1 and catalase proteins were separated on a 4% to 20% Tris-Glycine gel (Life Technologies) by SDS-PAGE and transferred to a nitrocellulose membrane ...
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bioRxiv - Molecular Biology 2024Quote: ... and TRA-1-60 using the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit (Thermo Fisher Scientific, #A24881) as previously described [29].
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bioRxiv - Neuroscience 2024Quote: ... Nuclei were stained for 5 minutes with 1 µM 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; Invitrogen), coverslips were mounted on glass slides with mounting medium (Dako) ...
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bioRxiv - Genomics 2024Quote: ... Cells were split 1:4 every 5-7 days with 0.25% trypsin-EDTA (Gibco, cat. no. 25200056).
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bioRxiv - Biophysics 2024Quote: ... 4-(2-hydroxyethyl)-1-piparazineethanesulphonic acid (HEPES) and tris(hydroxymethyl)aminomethane (Tris) were purchased from Fisher Scientific.
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bioRxiv - Immunology 2024Quote: ... and pMD2.G at a ratio of 4:3:1 using Lipofectamine 3000 kit (Thermo Fisher Scientific). 2D3 Jurkat 76.7 cells expressing human CD8+ and NFAT-GFP (Morimoto et al ...
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bioRxiv - Cell Biology 2024Quote: ... centrifuged for 1 min at 16000 rcf and directly loaded on 4-12 % NuPAGE gels (Thermo fisher). Gels were run in MOPS buffer (Thermo fisher ...
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bioRxiv - Developmental Biology 2024Quote: ... batches of 4 embryos were digested in 40µl of a solution of 2:1 0.25% Trypsin (Gibco): Accutase (StemCell Technologies ...
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bioRxiv - Biophysics 2022Quote: Isolated islets were stained with 4 mM Fluo-4 AM (Invitrogen, Carlsbad, CA) in imaging medium (125 mM NaCl ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 7-Diethylamino-3-(4'-Maleimidylphenyl)-4-Methylcoumarin (CPM) was purchased from Thermo Scientific Life Technologies (Grand Island ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 100 µl of assay buffer containing 4 µM Fluo-4-AM (Life Technologies), 0.02 % Pluronic F127 and 2.5 mM probenecid in HBSS-Ca2+ was added to each well and the plate was incubated at room temperature (RT ...
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bioRxiv - Biochemistry 2024Quote: ... as was 7- Diethylamino-3-(4’-Maleimidylphenyl)-4-Methylcoumarin (CPM) (Invitrogen™ D346) and CPM stock was prepared at 5 mg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... were loaded with cell-permeant 4 µM Fluo-4 AM (Invitrogen, F-14201) for 35 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 minutes followed by ice cold 4% PFA for 4 min with a flow rate of 6ml/min and post-fixed (overnight at 4°C) in 4% PFA (Thermo Fisher Scientific) in 1x PBS (pH 7.4) ...
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bioRxiv - Genetics 2021Quote: ... and incubated overnight at 4°C with mouse anti-H4K3me3 (Wako #305-34819 at 1:500 dilution in PBST) or rabbit anti-H3K4me2 (Invitrogen #710796 at 1:20,000) primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... Cells are differentiated for 4 days in embryonic body (EB) differentiation medium (DMEM/F12 [Hyclone], 20% FBS, NEAA [1×, GIBCO], GlutaMAX [1×, GIBCO], 0.1% beta-Mercaptoethanol [GIBCO]). For mixed embryonic body (EB ...
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bioRxiv - Developmental Biology 2022Quote: ... and incubated with Alexa-Flour-488 or 555 secondary antibodies (1:1000) along with 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, Camarillo, CA, 1:1000) for 1 hr at room temperature (RT) ...
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bioRxiv - Neuroscience 2022Quote: ... A 100 mM stock solution (in 0.2 M NaOH and 15% DMSO) of TCO*A-Lys was diluted 1:4 in 1 M HEPES (Thermo Fisher Scientific, cat. no. 15630056), and added to the medium at a final concentration of 250 μM ...
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bioRxiv - Genomics 2022Quote: ... at a bead-to-cell ratio of 1:1 in the presence of recombinant IL-4 (Fisher Scientific, #204-IL-010, 10 ng/ml) and anti-IFN-γ antibody (10 μg/ml) ...
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bioRxiv - Neuroscience 2021Quote: ... a stock solution of UAA (100 mM in 0.2 M NaOH containing 15% DMSO) was diluted 1:4 in 1 M HEPES (Thermo Fisher Scientific, cat. no. 15630080) and added to cells to a final concentration of 250 µM for TCOA-Lys and TCO4en/eq-Lys ...
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bioRxiv - Cell Biology 2022Quote: siRNA transfections were carried out using 20 pmol of siRNA (SI Table 1) targeting LTBP3 (siLtbp3-1-4) and lipofectamine RNAiMAX (Invitrogen, Waltham, MA; 13778-075) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were probed with respective primary antibodies overnight at 4°C: anti-ZO-1 (1:500, Invitrogen, San Diego, CA, USA, Cat# 61-7300), anti-VE-Cadherin (1:500 ...
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bioRxiv - Cancer Biology 2024Quote: ... were subjected to overnight incubation at 4°C with a rabbit monoclonal anti-ADRA2A antibody (Thermo Fisher Scientific, Catalog #14266-1-AP, 1:200). Signal amplification was facilitated using the Dako envision + system-HRP labeled polymer anti-rabbit antibody (Agilent Technologies ...