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Citations for Thermo Fisher :
6351 - 6400 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and the increasing fluorescence intensities were measured by the real-time PCR system 7500 Fast (Applied Biosystems, Thermo Fisher Scientific). Data indicated is as a relative ratio between minimum and maximum intensity of SYPRO orange from 25 to 95°C detected for each sample.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Relative quantitation (RQ) of mRNA expression was analyzed using QuantStudio™ Real-Time PCR Software v1.7.1 (Applied Biosystems, CA, USA).
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bioRxiv - Microbiology 2020Quote: Thermal shift/stability assays (TSAs) were performed using a StepOnePlus Real-Time PCR machine (LifeTechnologies) and SYPRO-Orange dye (emission maximum 570 nm, Invitrogen) as previously described37 with thermal ramping between 20 and 95°C in 0.3°C step intervals per data point to induce denaturation in the presence or absence of various carbohydrates as appropriate to the sulfatase being analysed ...
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bioRxiv - Molecular Biology 2019Quote: ... or for pre- and mature mRNAs (far-3, ZK970.7) or for pre-mRNA only (eft-3) and using StepOnePlus Real-time PCR Systems (Applied Biosystems) according to the suppliers’ protocols ...
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bioRxiv - Genomics 2019Quote: ... 300 nM of each primer and 3.75 μL of cDNA 1:4 diluted on a QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems). To evaluate the efficiency of the RT-qPCR assays ...
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jouvence, a new human H/ACA snoRNA involves in the control of cell proliferation and differentiationbioRxiv - Genetics 2020Quote: The differential expression of the snoRNA and selected genes was analysed by real-time PCR (QuantStudio 3, Applied Biosystems, France). The expression of the snoRNA was detected with TaqMan customized probes (Applied Biosciences ...
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bioRxiv - Genetics 2020Quote: ... Real time PCR was performed using both Taqman® (#4444557) and SYBR™ Green (#A25742, all purchased from Applied Biosystems) assays ...
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bioRxiv - Genetics 2021Quote: ... Real-time PCR amplification was carried out with the TaqMan Gene Expression Master Mix (Thermo Fisher Scientific, Waltham, MA, USA) on a LightCycler 480 (Roche ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... cycle number: 40) on an ABI V117 real-time PCR system with PowerUp SYBR Green Master Mix (ThermoFisher Scientific, A25742). The mRNA levels of the housekeeping gene GAPDH was used for normalization ...
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bioRxiv - Microbiology 2021Quote: ... qPCRs measurements were conducted in an Applied Biosystems Step One Real-Time PCR system (Life Technologies, Grand Island, NY, USA). The thermal cycling protocol was performed as follows ...
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bioRxiv - Molecular Biology 2020Quote: ... were used in a final reaction volume of 20 μL performed in a StepOnePlus Real-Time PCR System (Applied Biosystems).
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bioRxiv - Microbiology 2019Quote: ... was used to quantify the relative levels of cells using a Viia™7 Real-Time PCR Instrument (Applied Biosystems) with 50 ng gDNA template using the following primers ...
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bioRxiv - Systems Biology 2021Quote: ... Samples were loaded into 384 well plates via the Eppendorf EP Motion liquid handler and then run on a QuantStudio 12K Flex Real-Time PCR System (ThermoFisher) using TaqMan Universal Master Mix II no UNG kit (ThermoFisher 4440040) ...
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bioRxiv - Pathology 2021Quote: ... or on a BioRad CFX96 Real-Time System using the Power SYBR® Green PCR Master Mix (Applied Biosystems, 4367660). Target gene expression was normalized to the average of two housekeeping genes (Gapdh and Rn18s) ...
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bioRxiv - Biochemistry 2021Quote: Thermal shift/stability assays (TSAs) were performed using a StepOnePlus Real-Time PCR machine (LifeTechnologies) and SYPRO-Orange dye (emission maximum 570 nm, Invitrogen) as previously described28 with thermal ramping between 20 and 95°C in 0.3°C step intervals per data point to induce denaturation in the presence or absence of various carbohydrates as appropriate to the sulfatase being analysed ...
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bioRxiv - Physiology 2021Quote: ... Quantitative real time polymerase chain reaction (qRT–PCR) was performed using TaqMan_ Gene Expression Assays (all Life Technologies, Darmstadt, Germany). TaqMan_Gene Expression Master Mix and the StepOnePlusTM Real-Time PCR System (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... The qPCR reactions were performed with a QuantStudio™ 3 Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific, USA). For each instrumental run containing unknown samples ...
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bioRxiv - Immunology 2021Quote: ... Real time PCR was carried out in triplicate on a QuantStudio 6 Flex instrument (Applied Biosystems, Foster City, CA, USA). Raw Ct values were first normalized using the inter-plate calibrator UniSp3 ...
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bioRxiv - Microbiology 2021Quote: ... U.S.) according to the manufacturer’s instructions and quantified using ABI StepOnePlus Real-Time PCR System (Life Technologies, Foster City, USA). Purified amplicons were pooled in equimolar and paired-end sequenced (PE250 ...
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bioRxiv - Immunology 2020Quote: ... Quantitative real-time PCR was performed on Bio-Rad CFX thermal cyclers with TaqMan Gene Expression assays from Life Technologies (GAPDH ...
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bioRxiv - Immunology 2021Quote: ... Rps29 (forward 5’-GCAAATACGGGCTGAACATG-3’; reverse 5’-GTCCAACTTAATGAAGCCTATGTC-3’) by real-time PCR using TaqMan Gene Expression Assays (Applied Biosystems), Universal PCR Master Mix (Applied Biosystems ...
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bioRxiv - Immunology 2020Quote: ... qPCR reactions were run on a Step one Plus real-time PCR apparatus using the absolute quantification program (Applied Biosystems), with the following conditions for FAM-TAMRA probes ...
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bioRxiv - Neuroscience 2021Quote: ... The polymerase chain reaction and following allelic discrimination were performed on QuantStudio 5 Real-Time PCR System platform (Applied Biosystems).
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bioRxiv - Developmental Biology 2021Quote: ... Gene expression analysis was then performed by Real Time quantitative PCR (qPCR) using a QuantStudio 6 Flex thermocycler (Thermo Fisher). We used the following Taqman primers from Thermo Fisher (Supplementary Table 3) ...
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BET protein inhibition regulates macrophage chromatin accessibility and microbiota-dependent colitisbioRxiv - Immunology 2021Quote: ... Quantitative real-time PCR was performed using 10 ng cDNA with the PowerUp SYBR Green Master Mix (Applied Biosystems, A25741) and 10 μM of forward and reverse primers for Il12β (F ...
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bioRxiv - Genetics 2020Quote: ... The experiments were performed in triplicates on 96 well plates and used the StepOne™ Real-Time PCR System (ThermoFisher) where a standard curve was generated for each gene ...
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bioRxiv - Molecular Biology 2020Quote: ... and primer sets for gene expression and analyzed on a Applied Biosystems 7300 Real-Time PCR System (Thermo Fisher Scientific). Primers used in this study were listed in Table S2 ...
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bioRxiv - Microbiology 2020Quote: ... The qPCR reactions were performed with a QuantStudio™ 3 Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific, USA). For each instrumental run containing unknown samples ...
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bioRxiv - Molecular Biology 2022Quote: ... and set up with the 7500 Real Time PCR System software version 2.0 (Applied Biosystems, Life Technologies™, California, USA). The reactions underwent PCR amplification as follows ...
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bioRxiv - Neuroscience 2022Quote: ... Ss06901209_m1), fibulin-5 (FBLN5, Ss04805024_m11) and samples were run on a QuantStudio 6 Real-Time PCR system (Applied Biosystems #4485694) in technical and biological replicates (n = 3-5) ...
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bioRxiv - Biochemistry 2022Quote: ... PerfeCTa SYBR Green FastMix (Quantabio) and 0.5 μM of gene-specific primers in a 7500 Fast Real-Time PCR System (Applied Biosystems), calculating relative expression using the ΔΔCt method (Livak and Schmittgen ...
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bioRxiv - Bioengineering 2022Quote: ... Reactions were aliquoted into a 96 well plate for analysis with a StepOnePlus™Real-Time PCR system (Applied Biosystems). Reactions were initially heated to 95 °C for 20 s ...
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bioRxiv - Cancer Biology 2022Quote: ... qPCR was carried out on a QuantStudio 12K Flex Real-Time PCR System (Rockefeller University Genomics Resource Center, ThermoFisher Scientific) device with the following thermocycling conditions ...
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bioRxiv - Cancer Biology 2022Quote: ... qPCR was carried out on a QuantStudio 12K Flex Real-Time PCR System (Rockefeller University Genomics Resource Center, ThermoFisher Scientific) device with the following thermocycling conditions ...
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bioRxiv - Cancer Biology 2022Quote: ... qPCR was carried out on a QuantStudio 12K Flex Real-Time PCR System (Rockefeller University Genomics Resource Center, ThermoFisher Scientific) device with the following thermocycling conditions ...
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bioRxiv - Neuroscience 2022Quote: ... qPCRs were run using PowerUp SYBR green master mix on the QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems). Mouse primers used for qPCR:
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and incubated under heating from 25 °C to 99 °C in StepOne Plus Real-time PCR system (Thermo Fisher Scientific). The kinetics of fluorescent signals were analyzed to calculate Tm values in Protein Thermal Shift Software 1.3 (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... The loaded plate was amplified for 40 cycles via the ViiA 7 real-time PCR system (Thermo Fisher Scientific, Waltham). For each biological replicates (n = 3) ...
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bioRxiv - Plant Biology 2022Quote: ... Quantitative real-time PCR was performed on an ABI Quant Studio™ 6 Flex system (Applied Biosystems, Waltham, MA, USA) with SYBR Green PCR master mix (TaKaRa ...
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bioRxiv - Plant Biology 2022Quote: ... and reactions were performed in eight-link boards on QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems, USA) with cycling conditions (50 °C 2 min ...
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bioRxiv - Molecular Biology 2023Quote: ... was performed using qPCRBIO Probe Mix Hi-ROX (Nippon Genetics, Cat.-No. PB20.22-51) in 96-well plates with the StepOnePlus real-time PCR system (Applied Biosystems) under thermocycling conditions as described below.
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bioRxiv - Physiology 2023Quote: ... at 1,000g for 2min and then immediately loaded into the QuantStudio 6 and 7 Flex real-time PCR system (ThermoFisher Scientific). A two-step cycling protocol was implemented to collect cycle threshold (Ct ...
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bioRxiv - Developmental Biology 2022Quote: ... and TaqMan probes (Supplementary Table 4) in an ABI Prism 7900 system or QuantStudio6 Real-time PCR machine (Applied Biosystems). Gene expression normalisation was performed against combination of the housekeeping genes ...
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bioRxiv - Cancer Biology 2022Quote: ... for quantitative polymerase chain reaction (qPCR) at the manufacturer’s recommended settings in a StepOnePlus Real-Time PCR System (Applied Biosystems). The relative mRNA expression was calculated using the 2-ΔΔct method (Livak and Schmittgen ...
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bioRxiv - Cancer Biology 2024Quote: ... The ChIP library was validated using Agilent TapeStation and quantified using Qubit 2.0 Fluorometer as well as real time PCR (Applied Biosystems). The sequencing libraries were multiplexed and clustered on one lane of a flowcell ...
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bioRxiv - Physiology 2024Quote: ... Transcript mRNA levels were determined by qRT-PCR on the ABI Prism 7900HT Real Time System (Applied Biosystems, Carlsbad, CA), using SYBR green (Qiagen ...
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bioRxiv - Neuroscience 2024Quote: ... and resultant quantification threshold cycles (CT) were calculated using the default settings in the QuantStudio Real Time PCR Software v1.3 (Applied Biosystems) (Tables S21-S24) ...
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bioRxiv - Neuroscience 2024Quote: ... Genes were amplified using forward and reverse primers and detected using Power Up Sybr Green reagent in a Quant Studio 3 Real-Time PCR system (ThermoFisher). Ct values were calculated and relative express was quantified using GAPDH expression as a reference control ...
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bioRxiv - Genetics 2024Quote: ... DNA amplification and melt curve analysis were performed in an ABI7500 Fast real-time PCR instrument (Applied Biosystems, Foster City) with 95°C initial denaturation for 30 seconds followed by 40 cycles of denaturation at 95°C for 5 seconds and annealing and extension at 60°C for 30 seconds ...
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bioRxiv - Immunology 2024Quote: ... We performed reaction in triplicates with 10 µl total volume using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). We normalized qPCR signal from ChIP DNA by 2% DNA input using the formula % of input = 100*2ᶺ(Ct [input]-Log2(50 ...