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Citations for Thermo Fisher :
6551 - 6600 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Real-time Taqman PCR was performed on an ABI 7900HT with TaqMan Gene Expression Assays (Applied Biosystems, Life Technologies, Carlsbad, CA) for human DAGLB exon 9-10 (Hs00373700_m1) ...
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bioRxiv - Neuroscience 2021Quote: ... Real-time Taqman PCR was performed on an ABI 7900HT with TaqMan Gene Expression Assays (Applied Biosystems, Life Technologies, Carlsbad, CA) for human DAGLB exon 9-10 (Hs00373700_m1) ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA was used for qPCR using the PerfCTa SYBR Green FastMix (QuantBio) on a Step One Plus Real Time PCR System (Life Technologies) using primer pairs in Supplementary Table 4 ...
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bioRxiv - Molecular Biology 2020Quote: ... To quantify viral genome copy number total DNA was extracted from cells and subjected to qPCR on 7900HT Fast Real-Time PCR System (catalog no: 4329003, Thermo-Fisher Scientific) using Fast SYBR Green Master Mix (catalog no ...
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bioRxiv - Molecular Biology 2020Quote: ... and GADD45 were measured using a StepOne Plus real-time thermocycler with a Fast SYBR Green PCR master mix (4385612; Applied Biosystems) in the amygdala during early life and adulthood ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction mixtures containing SYBR Green were composed following the manufacturer’s protocol and then CT values were obtained using a qPCR platform (QuantStudio 6 Flex Real-Time PCR System, ThermoFisher Scientific). Real-time PCR data was monitored using QuantStudio 6 Design and Analysis Software Version 2.3 ...
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miR-125-chinmo pathway regulates dietary restriction dependent enhancement of lifespan in DrosophilabioRxiv - Molecular Biology 2020Quote: ... Each cDNA sample was diluted 1:25 and real-time quantitative PCR (qPCR) was performed in duplicate using miRNA-specific primers/probe on a Quant 6 studio Real Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... and subsequently used for qPCR with the PerfCTa SYBR Green FastMix (QuantBio, 95071-012) on a Step One Plus Real Time PCR System (Life Technologies) using primer pairs in Supplementary Table 3 ...
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bioRxiv - Cell Biology 2022Quote: ... using primer pairs that spans either introns or exons of the target genes with an a StepOnePlus Real-Time PCR System (Applied Biosystems). The PCR reactions were performed at 95 °C for 10 min followed by 45 cycles of 95 °C for 15 sec ...
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bioRxiv - Cell Biology 2022Quote: ... 900nM reverse primer and 250nM probe per reaction and 1μL template on a StepOnePlusTM real-time PCR system (Thermo Fisher Scientific). After a UNG incubation at 50°C for 2 minutes Initial denaturation at 95°C for 2 minutes was followed by 40 cycles of 95°C denaturation for 1 second and 60°C anneal-extension for 20 seconds ...
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bioRxiv - Immunology 2021Quote: ... was performed using Thunderbird SYBR qPCR Mix (QPS-201; TOYOBO) with the 7500 fast Real-time PCR system or Quant-Studio 3 (Applied Biosystems). For quantification of gene expression levels ...
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bioRxiv - Biochemistry 2019Quote: ... and fluorescence of the hydrophobic residue-binding dye SYPRO Orange (5000-fold dilution) measured on a Step One Plus Real-Time PCR instrument (Applied Biosystems). DSF measurements were performed for each protein in the absence and presence of SAM (150 μM ...
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bioRxiv - Physiology 2019Quote: ... mRNA levels of the two targets (GFAP and AQP4) by qPCR was performed with the QuantStudio 3 Real-Time PCR system (Applied Biosystems). Brain tissues of interest (WKY ...
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bioRxiv - Immunology 2020Quote: The expression of mRNA levels of IL-1β and SAA3 in BMDMs and lung homogenate cells was measured by Real-Time PCR from the total RNA using GAPDH as internal control with specific primer and probe sets (Applied Biosystems) as previously described (19).
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bioRxiv - Immunology 2019Quote: ... The Tmem119 gene expression was detected by real-time quantitative PCR using TaqMan Fast Advanced Master Mix (#4444557; Thermo Fisher Scientific) and Tmem119 TaqMan Gene Expression Assay (#Mm00525305_m1 ...
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bioRxiv - Biochemistry 2019Quote: ... Real-time RT-PCR was performed in duplicate with AccuPower 2X Greenstar qPCR Master Mix (Bioneer) on a 7900HT Fast Real-Time PCR System (Applied Biosystems). Data were normalized to CD14 ...
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bioRxiv - Biochemistry 2020Quote: Differential scanning fluorimetry (DSF or thermal shift assay) for the DHTKD1 and its variants was performed on QuantStudio 3 real-time PCR system (Applied Biosystems). Purified proteins were diluted to a final concentration of 0.5 mg/ml in 100 mM potassium phosphate ...
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bioRxiv - Genomics 2019Quote: ... Gene expression was determined via Taqman qPCR assays (Table S5) and run on the Applied Biosystems ViiATM 7 Real Time PCR system (ThermoFisher Scientific).
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bioRxiv - Genetics 2019Quote: ... Quantitative real-time PCR analysis using the comparative Ct method was performed on the Applied Biosystem Vii 7 system (ThermoFisher Scientific) with the Powerup SYBR Master Mix (ThermoFisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... height was recorded using a portable GPM anthropometer (Pfister Imports, New York, NY) and rs373863828 was genotyped using a TaqMan real-time PCR assay (Applied Biosystems), as previously described (Minster et al. ...
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bioRxiv - Microbiology 2019Quote: ... Each reaction was performed in technical triplicates and Ct values were generated on a Viia7 Real-time PCR machine (Life Technologies). Relative expression for Itgal was calculated by using ΔΔCt for each sample relative to B-actin ...
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bioRxiv - Immunology 2019Quote: ... cDNAs were mixed with primer pairs and amplified using SYBR green master mix and the 7900HT Fast real-time PCR system (Applied Biosystems). Iμ ...
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bioRxiv - Neuroscience 2019Quote: ... Primer efficiencies for real-time quantitative PCR (qPCR) experiments were calculated using cycle threshold (CT) values (SYBR® Green; Life Technologies) derived from five ...
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bioRxiv - Cancer Biology 2019Quote: ... and 0,25 μM of forward and reverse primers (Table S5) on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems).
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bioRxiv - Synthetic Biology 2019Quote: ... Library concentrations were then normalized to 4nM and validated by qPCR on a QuantStudio-3 real-time PCR system (Applied Biosystems), using the Kapa library quantification kit for Illumina platforms (Kapa Biosystems) ...
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bioRxiv - Molecular Biology 2019Quote: ... 2µL of diluted DNA sample was used as template for real time PCR analysis with PowerUp SYBR Green (Applied Biosystems, A25779). Data were normalized by β-actin as a loading control and then presented as relative normalized expression level.
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bioRxiv - Molecular Biology 2019Quote: All the qPCR analyses were performed in technical triplicates in 96-well plates on a QuantStudio 3 Real-Time PCR system (Applied Biosystems). The amplification efficiencies of each primer pair were determined by running standard curves with five points of a serial 2-fold dilution (ranging from 10 ng to 0.6 ng of total RNA input per reaction) ...
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bioRxiv - Physiology 2019Quote: ... DNA from all samples was diluted to 50ng/μl and subject to real-time PCR using SYBRgreen master mix (Applied Biosystems) using the following primers specific for nuclear and mitochondrial DNA.
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bioRxiv - Bioengineering 2019Quote: Matrix remodeling associated gene expression profiles were examined for MSC-seeded hydrogel specimens via QuantiStudio 7 Flex real-time PCR (Applied Biosystems). Frozen hydrogels isolated at days 0 ...
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bioRxiv - Genomics 2021Quote: ... A real-time qRT-PCR was performed with the gene-specific primers of selected genes using SYBR Green at the 7900 HT Fast Real-Time PCR System (Applied Biosystems) following the method established in our laboratory [21] ...
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bioRxiv - Genomics 2020Quote: ... Relative transcript levels of the β-lactamase encoding genes (blaOXA-1 and blaCTX-M) were assayed using TaqMan reagents on the StepOne Plus Real Time PCR machine (Applied Biosystems). The transcript level of blaOXA-1 and blaCTX-M were determined relative to the endogenous control gene rpsL (36 ...
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bioRxiv - Immunology 2021Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems) with the following thermal cycling conditions ...
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bioRxiv - Genomics 2019Quote: ... 55°C for 30 sec and 60°C for 1 min) was carried out in an ABI 7500 Fast Real Time PCR system cycler (Applied Biosystems). Samples were measured in three technical replicates ...
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bioRxiv - Developmental Biology 2020Quote: ... Table S4), PerfeCTa SYBR Green FastMix, and ROX (Quanta BioSciences, Gaithersburg, MD) on the 7300 Real-Time PCR System (Applied Biosystems). Relative expression for Nasrat ...
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bioRxiv - Microbiology 2021Quote: ... 56°C for 15 sec and 72°C for 30 sec in the Applied Biosystems 7500 or QuantStudio5 Real-Time PCR instruments (ThermoFisher Scientific). For absolute quantification ...
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bioRxiv - Neuroscience 2021Quote: ... The cDNA product was then subjected to quantitative real-time PCR (qPCR) using Fast SYBR™ Green Master Mix (Applied Biosystems) and gene specific primer pairs (Supplementary Table 9 ...
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bioRxiv - Microbiology 2021Quote: ... 20 ng cDNA in a 20 μL reaction and primers at 1 μM final concentration on a QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems). Primer specificity was ensured by designing primers to span exon-exon junctions ...
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bioRxiv - Cell Biology 2020Quote: ... The cDNA was then used for real-time qPCR using the SYBR Green qPCR Master Mix (Bi-make) with the QuantStudio 6 Flex Real-Time PCR system (Life Technologies). The mRNA levels of ß-actin were used as an internal control to normalize the mRNA levels of Hsp70s ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative real-time PCR Taqman primer probe sets specific for targets listed in Table S7 (Applied Biosystems, Foster City, CA, USA) were used ...
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bioRxiv - Bioengineering 2020Quote: ... The 5 μl reactions were run in triplicate in a 384-well plate inside a Viia7 Real Time PCR System (Applied Biosystems). The initial cycle was 50°C for 2 minutes and 95°C for 10 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... mRNA levels for the genes of interest were measured using a StepOnePlus Real-time PCR system and TaqMan gene expression assays (Applied Biosystems): β-actin (ID ...
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bioRxiv - Neuroscience 2020Quote: ... with specific primer pairs (table 1) were used for all qPCR assays and analyzed via QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). Technical and experimental triplicates were performed for all samples ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA synthesis was carried out and cDNA were analysed by qPCR on a StepOnePlus Real-Time PCR System (Thermo Fisher Scientific) using the SYBR Green Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... and then cDNA libraries were diluted to a standard concentration before running the qPCR assay using Power Sybr Green on ViiA7 Real-Time PCR System (Thermo Fisher) or QuantStudio 5 Real-Time PCR System (Thermo Fisher) ...
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bioRxiv - Genomics 2021Quote: ... This was measured by comparing cycle numbers against 1/3 of the maximum fluorescence intensity using real-time PCR graphs on ABI7900 (Applied Biosystems). After the additional PCR cycles ...
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bioRxiv - Microbiology 2020Quote: ... PCR was performed using the Applied Biosystems ViiA Real Time PCR System in a 20 µl reaction mix with 10 µl SYBR Green master mix (Applied Biosystems), 1 µmol each of the forward and reverse primers and 50ng of the template ...
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bioRxiv - Systems Biology 2021Quote: ... mRNA expression of genes of interest was analyzed with TaqMan real-time PCR in a ViiA™ 7 system (Applied Biosystems). RNA sequencing library were prepared with Illumina RNA-Seq with Poly-A selections ...
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bioRxiv - Plant Biology 2021Quote: ... and quantification PCRs were performed with ChamQ™ SYBR qPCR Master Mix (both from Vazyme Biotech co. ltd, Nanjing) on QuantStudio™ 7 Flex Real-Time PCR System (ThermoFisher). Three to four biological replicates from each of two to three independent trials were applied for each experiment ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative RT-PCR was performed using ChamQ Universal SYBR qPCR Master Mix (Vazyme) and a QuantStudioTM 6 Flex Real-Time PCR System (Applied Biosystems). Transcript abundances were calculated using the delta-Ct method47 and normalized to the transcript levels of ACTIN2 (AT3G18780) ...
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bioRxiv - Neuroscience 2020Quote: ... or VIC-Gapdh probe (#4331182) and FAM-Cstb probe (#451372) was undertaken using a 7500 Fast Real Time PCR System (Applied Biosystems. A 2-fold serial dilution sample of WT mouse cDNA was used as a standard.