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Citations for Thermo Fisher :
6601 - 6650 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... Knockdown efficiency in the T cells following shRNA transduction was determined by real-time quantitative PCR with TaqMan gene expression assays (Applied Biosystems) for TET2 (assay Hs00325999_m1 ...
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bioRxiv - Neuroscience 2020Quote: ... qRT-PCR reactions were carried out using TaqMan Gene Expression Master Mix (TaqMan) and an Applied Biosystems Step One Plus Real Time PCR Machine (Applied Biosystems) using previously described primer/probe sets (see Table S2 for sequences ...
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bioRxiv - Plant Biology 2020Quote: ... Primers and probes were designed using the Primer Express 3.0 software for Real-Time PCR (Applied Biosystems Foster City, California, USA), selecting the option MGB TaqMan probes with amplicon length 60 to 80 bp ...
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bioRxiv - Plant Biology 2020Quote: ... Primers and probes were designed using the Primer Express 3.0.1 software for Real-Time PCR (Applied Biosystems, Foster City, California, USA), selecting the option TaqMan® Quantification probes ...
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bioRxiv - Microbiology 2021Quote: ... 1.5 µl of 3 ng µl-1 cDNA was used as template in the QuantStudio 5 Real-Time PCR system (Applied Biosystems). Amplifications were performed with 5 μl of SYBR® green JumpStart Taq ReadyMix (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... RT-qPCR was performed with FAST SYBR Green Master Mix in a Step One Plus Real-Time PCR System (both Applied Biosystems). Primer sequences (IDT ...
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bioRxiv - Molecular Biology 2021Quote: ... Real-time PCR was performed using ChamQ SYBR Color qPCR Master Mix (Vazyme, China) on the ViiA7 system (Life Technologies, USA). The relative expression levels of target genes were calculated by the 2-ΔΔCT method ...
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bioRxiv - Molecular Biology 2021Quote: ... Real-time PCR was performed using ChamQ SYBR Color qPCR Master Mix (Vazyme, China) on the ViiA7 system (Life Technologies, USA). The relative expression levels of target genes were calculated by the 2-ΔΔCT method ...
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bioRxiv - Microbiology 2020Quote: ... The resulting cDNA was diluted 1:20 and subjected to semi-quantitative real time PCR using the SYBR Green reagent (Life Technologies) and specific primers ...
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bioRxiv - Cancer Biology 2021Quote: ... of gene expression was used and relative quantification was done using the equation 2−ΔΔCt (as per ‘Guide to Performing Relative Quantitation of Gene Expression Using Real-Time Quantitative PCR’ obtained from the Applied Biosystems website (http://www3.appliedbiosystems.com/cms/groups/mcb_support/documents/generaldocuments/cms_042380.pdf).
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bioRxiv - Cell Biology 2021Quote: ... qPCR was performed with the ViiA 7™ Real-Time PCR or ABI PRISM 7700 systems (Applied Biosystems, Foster City, CA). Methods are detailed in the online Supplementary material.
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bioRxiv - Cell Biology 2020Quote: ... Real-time RT-PCR was performed in triplicate using an Applied Biosystems 7500 Real-time PCR system and Taqman Gene Expression Assays (Thermo-Fisher Scientific). Data are presented as percentage of endogenous control (GAPDH ...
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bioRxiv - Physiology 2022Quote: ... All qPCRs were carried out in a 20 μL reaction on the qTOWER 2.2 real-time PCR system (Analytik Jena AG, Jena, Germany) using the PowerUp SYBR green master mix (Applied Biosystems, USA). Each PCR primer of a pair was designed to target different exons of the gene (Supplemental Table 3 ...
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bioRxiv - Neuroscience 2022Quote: ... Real-time quantitative PCR analyses were done by using SYBR™ Select Master Mix for CFX (appliedbiosystems by Thermo Fisher Scientific), a CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: ... 40 cycles of 95°C for 1 s and 60°C for 20 s using the QuantStudio 5 Real-Time PCR System (Applied Biosciences, ThermoFisher Scientific). Specificity was verified by melt curve analysis ...
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bioRxiv - Cell Biology 2022Quote: ... qPCR was performed with the THUNDERBIRD SYBR qPCR Mix (Toyobo) and analyzed using the StepOne real-time PCR system (Applied Biosystems). The primer sequences are shown in S3 Table.
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bioRxiv - Molecular Biology 2022Quote: ... The qPCR was performed in 96-well plate (Scientific Specialties) using THUNDERBIRD SYBR qPCR Mix (TOYOBO) in Applied Biosystems 7900HT Fast Real Time PCR System (Applied Biosystems). The relative quantity of the target expression was calculated by 2-ΔΔCt methods using SDS Software 2.4.2 (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was amplified using a StepOne Plus real-time PCR Thermocycler with TaqMan Gene Expression Master Mix (both Thermo Fisher Scientific) using universal cycling conditions ...
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bioRxiv - Immunology 2022Quote: ... in 384 well plates with 0.5 μM forward and reverse primers (sequences in Supplementary table 1) run on a Viia7 Real-Time PCR machine (Thermo Scientific). Fold induction of the target gene was calculated relative to GAPDH in human cells and Hprt in murine cells.
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bioRxiv - Microbiology 2022Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems) with the thermal cycling conditions ...
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bioRxiv - Microbiology 2022Quote: RT-qPCR for YFV detection was performed as previously described [17] using primers and probe targeting the YFV NS3 gene [23] on an ABI 7500 Fast Real-Time PCR System (Applied Biosystems). Absolute quantification was based on standard curves generated from 5-fold serial dilutions of YF17D cDNA with a known concentration.
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bioRxiv - Microbiology 2022Quote: ... 95°C 2 min, (95°C 15 sec., 60°C 20 sec.) x 40 cycles on a StepOnePlus Real-Time PCR system (Applied Biosystems). For a standard curve ...
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bioRxiv - Microbiology 2022Quote: ... Tagged cDNA was then diluted 1 in 10 and quantified using real-time quantitative PCR using Fast SYBR green master mix (Thermo Scientific). Primer pairs used were ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions were assembled in MicroAmp Optical 96-well reaction plates and carried out in a QuantStudio-7 Flex real-time PCR instrument (Applied Biosystems). PCR conditions were as follows ...
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bioRxiv - Neuroscience 2022Quote: ... Gene expression was quantified by real-time PCR with marmoset specific TaqMan Gene Expression Assays (Applied Biostems) and TaqMan Fast Advanced Master Mix (Applied Biosystems) on a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems).
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bioRxiv - Cancer Biology 2022Quote: ... qPCR was conducted in a Bio-Rad CFX96 real-time PCR system using TaqMan gene-specific primer/probe sets (Applied Biosystems) and SsoAdvanced master mix (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... The qPCR plate was sealed and read in the FAM and HEX channels using a QuantStudio™ 5 Real-Time PCR system (ThermoFisher) under the following cycling protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... A final cDNA concentration of 5ng/µl was used for semi-quantitative real time PCR analysis performed in Thermocycler ABI7500 Fast (Applied biosystems), using PowerUp SYBR Green Master Mix (Applied biosystems ...
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bioRxiv - Biophysics 2022Quote: The structural integrity of the protein at increasing temperature was performed using SYPRO Orange as external fluorophore in a 7500 Real-Time PCR System (Applied Biosystems). 20□μl samples were prepared at 5x SYPRO Orange fluorophore (Sigma) ...
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bioRxiv - Cancer Biology 2022Quote: ... mixed with indicated cDNAs and primers (primer sequences are listed in Supplemental Table S1) in a QuantStudio Real-Time PCR system (Applied Biosystems). Expression levels were normalized by 18S rRNA.
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bioRxiv - Cell Biology 2022Quote: ... Genotyping was performed using KASPar assays (LGC Genomics) in an Applied Biosystems 7500 Fast Real-Time PCR system (Thermo Fisher Scientific), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... The transcripts of lysosome biogenesis genes and their transcription factors were amplified from the cDNA in QuantStudio 6 Flex real-time PCR system (Applied Biosystems). As a control 18S rRNA or β-Actin were amplified along with the lysosomal genes ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative real-time PCR reactions were performed using Brilliant III Fast SYBR-Green QPCR Master Mix on an QuantStudio 3 real-time PCR instrument (Life Technologies) with the following program ...
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bioRxiv - Neuroscience 2019Quote: ... in the three brain regions was measured using a StepOne Plus real-time thermocycler with a Fast SYBR Green PCR master mix (4385612; Applied Biosystems). Primers were purchased from Qiagen or Eurofins Genomics and designed according to GenBank sequence information at the National Center for Biotechnology Information (NCBI ...
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African Swine Fever Virus CD2v protein promotes β-Interferon expression and apoptosis in swine cellsbioRxiv - Microbiology 2020Quote: ... IFN-β and ISGs mRNAs were quantified using ABI and QuantStudio-3 Real time PCR system (Applied Biosystems, Foster city, CA), Power SYBR Green PCR Master Mix (Applied Bio ...
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bioRxiv - Cancer Biology 2021Quote: ... The cDNA was then used as templates and qPCR was performed using the SYBR Green qPCR Master Mix (SolomonBio, QST-100) on the QuantStudio 3 Real-Time PCR system (Applied Biosystems). Primers used in qPCR were listed in Supplementary Table 8.
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bioRxiv - Immunology 2020Quote: ... Real-time PCR was performed using an UltraSYBR Mixture (Beijing CoWin Biotech, Beijing, China) on a ViiA 7 real-time PCR system (Applied Biosystems). Viral rRNAs were normalized to swine 28S rRNA expression ...
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bioRxiv - Cancer Biology 2020Quote: ... Data analysis was performed with the QuantStudio 6 and 7 Flex Real-Time PCR System Software v1.0 (Applied Biosystems, Life Technologies) using the ΔΔCt method.
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bioRxiv - Cell Biology 2021Quote: ... The resulting cDNA was amplified quantitatively using PerfeCTa SYBR Green Mix (Quanta) on a 7500 Fast Real-time PCR System (Applied Biosystems). The relative gene expression was determined using differences in Ct values between gene of interest and house-keeping control genes β-actin and HPRT1.
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bioRxiv - Biochemistry 2021Quote: Thermal unfolding experiments were carried out through preferential binding of a fluorophore to unfolded protein using a ViiA7 real-time PCR instrument (Applied Biosystems). In brief ...
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bioRxiv - Microbiology 2021Quote: RT-qPCR assays were performed using 5x HOT FIRE Pol EvaGreen qPCR Mix Plus (Solis Biodyne) in a QuantStudio 3 Real-Time PCR System (ThermoFisher Scientific) machine ...
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bioRxiv - Neuroscience 2021Quote: ... Cx3cr1 and 18S RNA (internal control) were measured by semiquantitative real-time PCR on an ABI Prism 7900 HT (Applied Biosystems).
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bioRxiv - Biochemistry 2021Quote: ... Murine PNPLA2 mRNA levels relative to HPRT transcript levels were measured by the QuantStudio 7 Flex Real-Time PCR System using Taqman® gene expression assays (PNPLA2, Mm00503040_m1; HPRT, Mm00446968_m1, Thermo Fisher Scientific). PNPLA2 relative expression to HPRT was calculated using the comparative ΔΔCt method.27
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bioRxiv - Microbiology 2021Quote: Real-time PCR was performed using primers (Integrated DNA Technologies, Coralville, IA, USA) and probes (Thermo Fisher Scientific, Waltham, MA, USA) with sequences and concentrations listed in Table 1 in combination with QuantiTect Multiplex PCR Kit (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... Data analysis was performed with the QuantStudio 6 and 7 Flex Real-Time PCR System Software v1.0 (Applied Biosystems, Life Technologies) using the ΔΔCt method.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... analysis was performed using a QuantStudio 12 K Flex real-time PCR system (Applied biosystem) and Luminaris Probe qPCR Master Mix (Thermo Scientific), following the manufacturers’ instructions ...
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bioRxiv - Immunology 2022Quote: ... The reactions were set up using the manufacturer’s guidelines and run on a StepOnePlue Real-Time PCR Machine (Thermo Fisher Scientific). Values are represented as the difference in cycle threshold (Ct ...
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bioRxiv - Immunology 2022Quote: ... cDNA was amplified using the Fast SYBR Green Master Mix on a QuantStudio 7 Flex Real Time PCR Systems (Applied Biosystems). The sequences of primer pairs (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... and Il-1β (in aortic CD3- cells) mRNA levels were assessed by qPCR using QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems), and initial differences in RNA quantity were corrected for using the housekeeping genes 36b4 and Cyclophilin B.
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bioRxiv - Developmental Biology 2022Quote: ... with cDNA (diluted 1:5) and gene-specific primers (Key Resources Table) on a QuantStudio 3 Real-Time PCR System (Applied Biosystems) in triplicate ...