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Citations for Thermo Fisher :
6251 - 6300 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... followed by sequencing via SuperScript III One-Step RT-PCR System with Platinum Taq RT-PCR (Life Technologies, USA) conducted by Life Technologies Biotechnology (Shanghai ...
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bioRxiv - Microbiology 2020Quote: ... RT-PCR was performed using a Superscript III one-step RT-PCR system with platinum Taq DNA polymerase (Invitrogen) with four sets of specific primers ...
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bioRxiv - Microbiology 2020Quote: ... RT-PCR was performed using a Superscript III one-step RT-PCR system with platinum Taq DNA polymerase (Invitrogen) with primers covering the entire S segment region according to a previously reported study [9] ...
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bioRxiv - Microbiology 2020Quote: ... The RT-PCR was performed using the SuperScript III Platinum One-Step Quantitative RT-PCR system (Invitrogen, Carlsbad. CA) on a LightCycler 96 or LightCycler 480 instrument (Roche Diagnostics ...
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bioRxiv - Microbiology 2022Quote: ... The RT-PCR was performed using the SuperScript III Platinum One-Step Quantitative RT-PCR system (Invitrogen, Carlsbad, CA) or Taqman Fast Virus 1-step master mix (Applied Biosystems ...
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bioRxiv - Plant Biology 2020Quote: ... Reactions were placed in 96-well plates and read with the Fast 7500/7500 Real-Time PCR System (Applied Biosystems). Data analysis was performed using the Fast 7500/7500 RealTime PCR System built-in software (Applied Biosystems).
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bioRxiv - Cancer Biology 2021Quote: ... and qPCR reactions were performed using power SYBR green master mix on a StepOnePlus real-time PCR system (Applied Biosystems). Relative gene expression levels were normalized to GAPDH.
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bioRxiv - Cell Biology 2020Quote: ... The target genes were amplified and detected with an Applied Biosystems QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific) and the gene expression levels were normalized to β-Actin ...
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bioRxiv - Genetics 2021Quote: ... DSF was performed by heating samples containing 10X SYPRO Orange in 1°C increments from 25°C to 100°C using the StepOne real-time PCR machine and using the Protein Thermal Shift software (ThermoFisher) to fit the data.
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bioRxiv - Genetics 2021Quote: ... was used to quantify extracts according to the manufacturer’s recommended protocols using the Applied Biosystems’ 7500 real-time PCR instrument (ThermoFisher Scientific).
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bioRxiv - Genetics 2020Quote: ... 10 ng cDNA was used as the template for each real-time PCR reaction by SYBR Green mix (Thermo Scientific) with the Applied Biosystems 7300 ...
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bioRxiv - Developmental Biology 2020Quote: ... Chromatin-immunoprecipitated DNA was quantified by qPCR using SYBR Green and a 7900HT Fast Real-Time PCR machine (Applied Biosystems) and primers specific for Eif4ebp1 ...
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bioRxiv - Developmental Biology 2020Quote: ... and carried out for three biological replicates with measurements taken from three technical replicates on an Applied Biosystems 7300 Real Time PCR system (Thermofisher). Mutants and siblings were then sorted by comparing their amplification curves upon qPCR with the WT and mutant set of primers (Ctmutant – CtWT).
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bioRxiv - Developmental Biology 2020Quote: ... 50 ng template was used per reaction with SYBR Green Mastermix in ViiA 7 Real-Time PCR System (Applied Biosystems). As endogenous control ...
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bioRxiv - Developmental Biology 2021Quote: ... Real time qPCR was carried out for three biological replicates with measurements taken from three technical replicates on an Applied Biosystems 7300 Real Time PCR system (Thermofisher). After normalization with zebrafish elongation factor 1a (ef1a) ...
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Wnt-dependent activation of ERK mediates repression of chondrocyte fate during calvarial developmentbioRxiv - Developmental Biology 2021Quote: ... Relative mRNA expression was quantified using 4 ng of cDNA on a StepOne Plus Real-Time PCR System (Life Technologies) and the ΔΔCT method [45] ...
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bioRxiv - Developmental Biology 2021Quote: ... qPCR was then performed for at least 3 biological replicates using Biozym Blue S’Green qPCR Mix Separate ROX (No. 331416XL) on QuantStudio 7 Flex Real-Time PCR System from Applied Biosystems. Pax3 fold change was calculated from ΔCt using Gapdh as housekeeping gene (2^-(ΔCt)) ...
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bioRxiv - Neuroscience 2020Quote: ... qPCR reactions were performed in triplicate for each Taqman assay of interest on a QuantStudio 3 Real Time PCR Machine (ThermoFisher).
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bioRxiv - Genomics 2020Quote: ... qPCR was performed in duplicates in 384-well optical plates in a QuantStudio 5 Real-Time PCR system (ThermoFisher Scientific) using default settings ...
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bioRxiv - Immunology 2021Quote: Multiplex quantitative polymerase chain reaction (qPCR) was carried out using a QuantStudio 6 Flex Real-Time PCR System (ThermoFisher Scientific) as described previously [23] ...
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bioRxiv - Microbiology 2019Quote: ... Viral titers were determined using the SuperScript® III Platinum® One-Step Quantitative real-time PCR system (Life Technologies) with the following probe and primers ...
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bioRxiv - Microbiology 2019Quote: ... Reactions were run with 3 technical replicates each on a ViiA7 Real-time PCR system (Thermo Fisher, Waltham, MA, U.S.A.) programmed as follows ...
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bioRxiv - Neuroscience 2019Quote: ... and 0.5 μM of gene-specific primers (Table S2) in a 7500 Fast Real-Time PCR System (Thermo Fisher Scientific), using a two-step cycling protocol (3 seconds at 95°C ...
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bioRxiv - Zoology 2019Quote: Gene expression levels were analysed with quantitative polymerase chain reaction (qPCR) using a StepOnePlus Real-Time PCR System (Applied Biosystems) as described previously [25] ...
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bioRxiv - Physiology 2020Quote: ... Measurements of mRNA were performed with SYBR green chemistry using the QuantStudio 6-flex Real-time PCR System (ThermoFisher Scientific). All data were normalized to either Hprt or Actinb by the ΔC(t ...
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bioRxiv - Molecular Biology 2019Quote: ... was used for qPCR and implementing on an Applied Biosystems 7500 Real-Time PCR system (Applied Biosystems, Foster City, CA). The reactions were performed in a 20 µl volume of a mixture containing 1 µl of cDNA template ...
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bioRxiv - Genomics 2021Quote: ... Analysis of gene expression was performed with a STEP ONE PLUS Real Time PCR system (Applied Biosystems Japan, Tokyo, Japan) using SYBR GREEN PCR Master Mix (Applied Biosystems Japan ...
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bioRxiv - Microbiology 2020Quote: ... Relative quantification of genomic DNA or cDNA was performed on a 7900HT Fast Real-Time PCR System (ThermoFisher Scientific, 4329003) using sequence specific primers (Extended Data Table 1 ...
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bioRxiv - Plant Biology 2020Quote: ... PCR reactions were performed using HOT FIREPol EvaGreen qPCR Mix Plus (Solis Biodyne; www.sbd.ee) and analyzed in an ABI 7500 Real-Time PCR System (Applied Biosystems; www.appliedbiosystems.com). Two technical replicates were analyzed for at less three biological samples ...
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bioRxiv - Neuroscience 2021Quote: ... and gene specific primer pairs (Supplementary Table 9) on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems). Relative mRNA expression levels were calculated using the 2−ΔΔCt method and RPL18A as the house-keeping gene.
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bioRxiv - Neuroscience 2020Quote: ... nuclease-free water and stored at −20 °C prior to use in real-time Taqman PCR reactions (ThermoFisher, Carlsbad, CA). The resulting cDNA was loaded into a Mouse Inflammation array card (ThermoFisher ...
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bioRxiv - Biochemistry 2020Quote: Thermal shift/stability assays (TSAs) were performed using a StepOnePlus Real-Time PCR machine (LifeTechnologies) and SYPRO-Orange dye (emission maximum 570 nm, Invitrogen) with thermal ramping between 20 and 95°C in 0.3°C step intervals per data point to induce denaturation of purified ...
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bioRxiv - Neuroscience 2021Quote: ... using 1ng/μL of cDNA according to the manufacturer’s protocol with a QuantStudio 12 K Flex Real-Time PCR System (Applied Biosystems). The following primers specific for mouse were used:
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bioRxiv - Cell Biology 2021Quote: ... The reaction was performed using a total volume of 15 μL in a Step-One Plus Real-Time PCR system (ThermoFisher), cycling conditions were as following ...
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bioRxiv - Neuroscience 2020Quote: ... Captured DNA (methylated) and input DNA were quantified by real-time quantitative PCR using the Applied Biosystems SYBR Green system (ThermoFisher) and the ABI 7500 Fast Real-Time PCR System were used to detect the target ...
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bioRxiv - Plant Biology 2021Quote: ... Transcripts of the respective genes were quantified using real-time quantitative PCR with SYBR Green reagents and procedures (Applied Biosystems or SensiFAST SYBR DNA polymerase No-ROX Kit) ...
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bioRxiv - Microbiology 2020Quote: ... The oligonucleotides used for qPCR are listed in Table S2 and qPCR was carried out in triplicate using a 7500 Real-Time PCR System (Thermofisher). Negative controls lacking template or RNA incubated without reverse transcriptase were included ...
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bioRxiv - Cell Biology 2021Quote: qPCR analysis was performed using the LabTaq Green Hi Rox (Labtech) following manufacturer’s instructions on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) and primers listed in Supplementary Table S4.
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bioRxiv - Plant Biology 2020Quote: ... qPCRs were carried out on an Applied Biosystems QuantStudio 7 Real-Time PCR Systems (Applied Biosystems, Foster City, California, USA) using isolated genomic DNA as template ...
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bioRxiv - Microbiology 2020Quote: ... qPCR was performed on a 7500 Fast Real Time PCR system and the results analyzed using 7500 software v 2.3 (Applied Biosystems). The competition index (CI ...
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bioRxiv - Microbiology 2021Quote: ... on a 7300 Real Time PCR System and 7300 System SDS RQ Study Software (Applied Biosystems, Beverly Hills, CA, USA). Melt curve analyses were used to rule out secondary products and primer dimer formation ...
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bioRxiv - Microbiology 2021Quote: ... The total load of bacteria and bacterial families were measured on the 7500 Fast Real-Time PCR system (Applied Biosystems) in 10 μL reactions using Fast SYBR™ Green PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative real-time PCR was then performed with SYBR Green (PowerUp™ SYBR™ Green Master Mix, Thermo Fisher Scientific) in a volume of 10 µl/well containing 1µl of reverse transcribed cDNA product from above ...
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bioRxiv - Microbiology 2021Quote: ... All real-time qPCR reactions were carried out using a 7500 Real-Time PCR System and data were analysed using the 7500 Software version 2.3 (Applied Biosystems). The endogenous control gene was rpoB for M ...
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bioRxiv - Physiology 2021Quote: ... Transcript mRNA levels were determined by qRT-PCR on the ABI Prism 7900HT Real Time System (Applied Biosystems, Carlsbad, CA), using SYBR green (Qiagen ...
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bioRxiv - Plant Biology 2021Quote: ... Spain) in a total volume of 20 μl using an Applied Biosystems 7500 Real-Time PCR System (Thermo Fisher Scientific). Data are the mean of three replicates ...
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bioRxiv - Plant Biology 2021Quote: ... qPCR was carried out using the SYBR Green master mix on a QuantStudio 3 Real-Time PCR System (Applied Biosystems) using primers listed in Supplemental Table 1 ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative PCR was performed using previously described gene specific primers in an ABI7300 Real Time PCR system with SYBR Green Master Mix (Invitrogen) (12).
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bioRxiv - Cancer Biology 2020Quote: ... mRNA expression was performed by real-time quantitative PCR reactions using Fast SYBR™ Green reaction mix (Thermo Fisher Scientific) and achieved on an Applied Biosystems 7500 Fast Real-time PCR system ...
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bioRxiv - Cancer Biology 2021Quote: ... We subject the mixture to a cycler for realtime amplification 7500 Real Time PCR Cycle (Applied Biosystems, Carlsbad, CA, USA), cycling at 95°C for 10 minutes ...