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Citations for Thermo Fisher :
6001 - 6050 of 10000+ citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... Real-Time PCR was performed with ABsolute Blue QPCR Mix low ROX 1X (Thermo Fisher Scientific, Waltham, MA, USA) and 2.5 µL of cDNA under the following thermal conditions ...
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bioRxiv - Microbiology 2022Quote: ... All qPCR reactions were run and analyzed on the QuantStudio 3 Real-Time PCR System with analysis software v1.5.1 (Applied Biosystems). Each timepoint was collected in biological triplicates with qPCR reactions from each experimental sample done in technical triplicates.
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bioRxiv - Neuroscience 2021Quote: ... following titration with real-time PCR (rAAV titers about 1.0–6.0 × 1012 viral genomes/mL, TaqMan Assay, Applied Biosystems). To visualize amyloid plaques ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was performed by using an ABI Prism® 7500 (Applied Biosystems) or Thermal Cycle Dice Real Time System (TP850 ...
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bioRxiv - Molecular Biology 2021Quote: ... Datasets were collected on an Applied Biosystems™ QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher Scientific). The cycling conditions were as follows ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantification of gene expression was performed using a 7900 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). Threshold values (CT ...
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bioRxiv - Molecular Biology 2020Quote: The enrichment of specific DNA fragments was analysed by real-time PCR using a QuantStudio 3 Cycler (Applied Biosystems). The results of chromatin immunoprecipitation are presented as a percentage of input genomic DNA after triplicate PCR measurements ...
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bioRxiv - Immunology 2021Quote: ... Targeted qPCR was performed using the QuantStudio 6 Real-Time PCR system using the following Taqman Primers (Applied Biosystems): BCL6 (Hs00153368_m1) ...
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bioRxiv - Cell Biology 2019Quote: ... Real-time PCR for Saa3 was performed using the TaqMan® assay (Applied Biosystems®, Grand Island, NJ, USA), catalogue number Mm00441203_m1 – Saa3 and β-actin (Actb) ...
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bioRxiv - Genomics 2019Quote: ... Real-time PCR reactions were performed on a 1/40 cDNA dilution using Taqman Assays-on-Demand (Applied Biosystems) and Kapa Probe Fast Mastermix (Kapa Biosystems ...
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bioRxiv - Cell Biology 2019Quote: ... Reactions were run on QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems, part of Life Technologies Corporation, CA) according to the manufacturer’s recommendations using a melt protocol in 0.05-degree/sec increments from 25°C to 95 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... according to manufacturer’s instructions in a 7900HT Fast-Real Time PCR System Instrument (Applied Biosystems, Grand Island, NY, USA). Primer pairs for the individual genes were obtained from the bioinformatically validated QuantiTect Library and are as follows ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the level of P16 methylation was determined using an Applied Biosystems 7500 Real-Time PCR System (Applied Biosystems, USA) and normalized to that of the internal control gene ...
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bioRxiv - Neuroscience 2021Quote: ... Analysis of expression profiles was done using StepOnePlusTM real-time PCR system (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) using the 2−ΔΔCT method56.
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bioRxiv - Neuroscience 2021Quote: ... Analysis of expression profiles was done using StepOnePlusTM real-time PCR system (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) using the 2−ΔΔCT method56.
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bioRxiv - Bioengineering 2021Quote: ... rAAV serotypes 1 and 2 viral particles were generated as described previously [40] and purified by AVB Sepharose affinity chromatography [41] after titration with real-time PCR (each titer 1.0 – 6.0E12 viral genomes/ml; TaqMan Assay; Applied Biosystems). For stereotaxic injection of rAAVs and the cranial window implantation ...
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bioRxiv - Bioengineering 2020Quote: ... The melting temperature of the different fusion proteins was determined using a StepOne real-Time PCR system (Applied Biosystems) in combination with the Protein Thermal Shift™ kit (Applied Biosystems).
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bioRxiv - Cancer Biology 2021Quote: Quantitative real-time PCR for ChIP assays (ChIP-qPCR) was performed using the SYBR Green Master Mix (Thermo Scientific) along with target-specific primers in optical 96-well plates on ABI StepOne Plus Thermocycler (Applied Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... Quantitative real-time PCR assays were performed in Maxima SYBR Green/ROX qPCR master mix (Thermo Fisher Scientific, K0222) using a Thermo Fisher Scientific QuantStudio 3 Real Time PCR system under the following sequence of conditions ...
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bioRxiv - Bioengineering 2021Quote: ... The cDNA was analyzed on an Applied Biosystems 7900HT Fast Real-Time PCR System (Applied Biosystems, Foster City, CA) in a 384-well microplate with the TaqMan™ Universal PCR MasterMix (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... The qPCR was performed by QuantStudio™ 6 Flex Real-Time PCR machine (Applied Biosystems, Foster City, CA, USA). Elongation Factor 1α (CqEF1α)(XM_021888526.1 ...
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bioRxiv - Microbiology 2020Quote: ... Forty cycles of amplification at 60 °C were conducted in a QuantStudio 3 Real-Time PCR System (Applied Biosystems). Amplification curves were analyzed using QuantStudio Design & Analysis Software v1.5.1 ...
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bioRxiv - Plant Biology 2021Quote: ... PR1 expression was detected using primers qPR1_F and qPR1_R by quantitative real-time PCR using Maxima SYBR green mix (Thermo Fisher) and the AriaMx Real-Time PCR system (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... and quantified by real-time PCR with SYBR Green (DotScientific) using the one-step protocol QuantStudio 3 (ThermoFisher Scientific). Relative expression was calculated using the ΔΔCT method ...
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bioRxiv - Microbiology 2021Quote: ... and quantified by real-time PCR with SYBR Green (DotScientific) using the one-step protocol QuantStudio 3 (ThermoFisher Scientific). Relative genomes were calculated using the ΔCT method ...
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bioRxiv - Microbiology 2021Quote: ... Absolute numbers of copies of proviral DNA were determined by real time PCR using custom made probes (Thermo Fisher). To obtain absolute proviral DNA copy numbers ...
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bioRxiv - Microbiology 2021Quote: ... Virus was quantified using real-time PCR using SYBR Premix Ex Taq™ (Applied Biosystems, Thermo Fisher Scientific, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Virus was quantified using real-time PCR using SYBR Premix Ex Taq™ (Applied Biosystems, Thermo Fisher Scientific, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Real-time PCRs were carried out on a StepOne device (Applied Biosystems, Thermo Fisher Scientific, Foster City, CA, USA) with 59 HOT FIREPolR Eva-Green qPCR Mix Plus (ROX ...
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bioRxiv - Cell Biology 2019Quote: ... The assays were carried out in a 7900HT Fast Real-Time PCR system (Applied Biosystems, Foster City, CA, USA), and the cycling conditions were as follows ...
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bioRxiv - Neuroscience 2020Quote: ... Quantitative real time PCR were performed using Taqman gene expression assays and gene expression master mix (Applied Biosystems, #4369016). The changes in gene expression were normalized to β-actin or glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
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bioRxiv - Microbiology 2021Quote: ... and 1 min at 60 °C cycle of 50 °C with a 7500 Real-Time PCR System (Applied Biosystems). The following Taqman primer and internal probes were used:
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bioRxiv - Physiology 2021Quote: ... Experiments were run on an Applied Biosystems 7500 Fast Real-Time PCR System (Life Technologies Corporation, Carlsbad, CA, USA). Primers were designed using Primer 3 software on different exons to exclude any DNA contamination ...
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bioRxiv - Microbiology 2021Quote: ... The reactions were performed on a StepOnePlus™ Real-Time PCR System in a 96-well plate (Thermo Fisher) as previously described 75.
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bioRxiv - Cancer Biology 2021Quote: ... cDNA samples were diluted 5-fold and it was performed with StepOne Plus Real Time PCR System (Applied Biosystems). The FastStart Universal SYBR Green Master Mix (Roche ...
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bioRxiv - Genetics 2022Quote: ... The validation of KASP markers was performed on the 96-well StepOnePlus™ Real-Time PCR System (Thermo Fisher Scientific Inc. ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time PCR was performed in a 10-μl reaction volume using Fast SYBR Green Master Mix (Applied Biosystems) and the concentration of primer is 100nM-300nM ...
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bioRxiv - Cell Biology 2022Quote: ... The qPCR reactions were performed in triplicate using a QuantStudio 5 Real-time PCR System (Thermo Fisher, cat#A34322) using the following thermocycling conditions ...
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bioRxiv - Cell Biology 2022Quote: mRNA levels of the genes of interest was measured using the StepOnePlus Real Time PCR System (Applied Biosystems, UK). cDNA was synthesized from total RNA using the AccuPower RT PreMix (Bioneer ...
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bioRxiv - Molecular Biology 2022Quote: ... qPCR was performed on triplicates of cDNA samples by using QuantStudio® 3 Real- Time PCR System (Applied Biosystems), Fast SYBR reaction mix (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: The primers used for real-time PCR were designed using PrimerExpress™ software (Applied Biosystems; see Supplemental Table S3). 800 ng total RNA were reverse transcribed to cDNA using High-Capacity cDNA Reverse Transcriptase kit (Ambion ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by real-time quantitative PCR analyses of the OXTR on a QuantStudio 6 qPCR platform (Life Technologies, USA) using the appropriate Taqman gene expression array (Hs00168573_m1 ...
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HNRNPA2B1 controls an unfolded protein response-related prognostic gene signature in prostate cancerbioRxiv - Cancer Biology 2022Quote: ... Binding of SYBR green to DNA was analysed in a QuantStudio 5 Real-Time PCR system (Thermo Fisher Scientific). Reaction conditions were as follows ...
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bioRxiv - Immunology 2022Quote: ... The qPCR was then carried out on a QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA). SARS-CoV-2 genomic (orf1a ...
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bioRxiv - Physiology 2022Quote: ... A final concentration of 0.133ng/μL was used per real time PCR reaction with InVitrogen SYBR green (ThermoFisher, 4309155) and processed on a ViiA 7 RT-PCR system ...
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bioRxiv - Developmental Biology 2022Quote: ... was used to detect amplification of the target sequences on a QuantStudio 5 Real-Time PCR system (Applied Biosystems). Data are represented by boxplots as above ...
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bioRxiv - Cell Biology 2022Quote: ... Realtime qPCR was performed in StepOne™ Real-Time PCR System using SYBR green master mix (Applied Biosystems™). Primers used for RT-qPCR are listed in Table S2.
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bioRxiv - Microbiology 2022Quote: ... The reactions were then run on a 7500 Fast Dx Real-Time PCR Instrument (4357362R, Applied Biosystems, Waltham, MA). For qPCR for murine GAPDH ...
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bioRxiv - Bioengineering 2022Quote: ... with a total reaction volume of 12.5 μL using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). Gene specific primers were used to amplify GDNF (forward sequence 5-GCAGACCCATCGCCTTTGAT-3 and reverse 5-CCACACCTTTTAGCGGAATGC-3).
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bioRxiv - Cell Biology 2022Quote: ... The qPCR reactions were performed in triplicates using a QuantStudio 5 Real-time PCR System (Thermo Fisher, cat#A34322) using the following thermocycling conditions ...