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Citations for Thermo Fisher :
6201 - 6250 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... samples were blotted at 100% humidity for 3 s (13°C, 0 s drain time, blot force -3 to +2) with a Vitrobot Mark IV (Thermo Fisher Scientific) and plunged into liquid ethane ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA was isolated from 6 pairs of P21 mouse testes (3 pairs of wild type and 3 pairs of Mov10-/-) using TRIzol reagents (Thermo Fisher Scientific). 1 μg of total RNA from each sample was used to generate RNA-seq libraries using TruSeq Stranded mRNA Library Preparation Kit Set A (Cat ...
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bioRxiv - Biochemistry 2023Quote: The ibpA 5’ UTR-gfp mRNA produced from CUGA®7 in vitro transcription kit (Nippon Gene) was attached to a 3’-terminal biotinylated nucleotide using Pierce™ RNA 3’ End Biotinylation kit (Thermo Scientific). The biotin-labeled mRNA (0.1 µM ...
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bioRxiv - Cell Biology 2023Quote: ... 5’-GAGACCCUAUCCGUGAUUAtt-3’ and antisense: 5’- UAAUCACGGAUAGGGUCUCtt-3’ (Silencer Select, rat negative control #1; scrambled siRNA and all siRNAs were from Ambion, Life Technologies). Transfection complexes were prepared in accordance with the instructions provided by the manufacturer and added to 2×105 cells seeded per well in 24-well plates.
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bioRxiv - Cell Biology 2023Quote: ... Treatments were incubated 2 hours before addition of the MTS (3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) reagent (Thermo-Fisher Scientific, Waltham, MA) and incubation was continued another 1.5 hours ...
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bioRxiv - Physiology 2023Quote: ... Digitonin solubilized mitochondria were loaded 3-12% Bis-Tris Invitrogen™ Novex™ NativePAGE™ 3-12% acrylamide gel (1mm) (Invitrogen, BN2011BX10) using the anode running buffer (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Four microliters of LotA7-544 was loaded onto a glow-discharged grid and blotted for 3 s with blot force 3 using Vitrobot Mark IV (Thermo Fisher, U.S.A.).
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bioRxiv - Cell Biology 2023Quote: Supernatants of cell culture medium collected during medium exchanges were analysed for albumin (10242, Diagnostic Systems) and 3-beta-hydroxybutyrate (Autokit 3-HB, Fujifilm Wako) on an Indiko Plus chemical analyzer (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was lawned with goat anti-human IgG Fc (50 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was coupled directly to mAbs diluted to 10 µg/mL in 10 mM sodium acetate (pH 4.5 ...
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Neuroscience 2023Quote: ... Neuron-cancer cocultures were quantified for neuronal outgrowth by identical image generation and processing with additional pre-incubation (3 drops/mL for 90 min at 37◦C) of NucRed Dead 647 ReadyProbes™ Reagent (TO-PRO-3 iodide) (Invitrogen, #R37113) for dead cells and NucBlue Live ReadyProbes™ Reagent (Hoechst 33342 ...
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bioRxiv - Microbiology 2024Quote: ... total RNA was harvested from a 10 ml parasite culture (5% hematocrit; >3% parasitaemia) by resuspending the pelleted RBCs in 3 ml pre-warmed Trizol (Invitrogen #15596-018). After a 5 min incubation step at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... on a QuantStudio 3 real-time PCR instrument (ThermoFisher). Values were normalized to the housekeeping gene SAND (At2g28390) ...
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bioRxiv - Biophysics 2021Quote: ... 3-(N-maleimidylpropionyl)biocytin (MBP) was obtained from Invitrogen and the HRP-conjugated antibody from eBioscience ...
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bioRxiv - Cell Biology 2020Quote: ... separated on a 3-8 % Tris-Acetate gel (Invitrogen) by SDS-PAGE ...
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bioRxiv - Cell Biology 2020Quote: ... and Y14 siRNA (5’-GGGUAUACUCUAGUUGAAUUUCAUAUUCAACUAGAG-3’) with Lipo2000 (Invitrogen) in Optimem (Gibco) ...
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bioRxiv - Cancer Biology 2020Quote: ... and PC-3 cells were cultured in RPMI1640 (Gibco) with 10% FBS and 1% Antibiotic-Antimycotic ...
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bioRxiv - Microbiology 2020Quote: ... epidermidis (Thermo scientific; 3 μg/ ml in 1% BSA). MSC’s were labelled with anti-vimentin monoclonal antibody (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... counterstained with DAPI or TO-PRO-3 (Thermo Fisher), and coverslipped with Fluoromount-G mounting medium (Southern Biotech ...
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bioRxiv - Developmental Biology 2021Quote: ... anti–glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Thermo Fisher Scientific), anti-FLAG (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2021Quote: ... 1.1ul (4U) FastDigest-HindIII (prediluted 1/3) (Thermo Fisher) and 11.1ul 20X primer-probe mix ...
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bioRxiv - Cell Biology 2022Quote: ... in PBS with 3% bovine serum albumin (BSA) (ThermoFisher), 0.4% saponin ...
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bioRxiv - Molecular Biology 2020Quote: ... 3-8% Tris-Acetate gels (Thermo Fisher Scientific, EA0375PK2) were used ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 µL of Lipofectamine RNAiMAX Reagent (Invitrogen, 13778-150) was diluted in 50 µL of Opti-MEM (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... anti-desmoglein-3 mouse monoclonal (Invitrogen, Carlsbad, CA, USA), anti-desmoglein-1 rabbit polyclonal (Abclonal ...
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bioRxiv - Genomics 2019Quote: ... and performing qPCR on a QuantStudio 3 (Thermo Fisher). The number of cycles was determined by cycle threshold value corresponding to the ½-maximum of amplification saturation on a linear curve ...
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bioRxiv - Molecular Biology 2020Quote: ... dialysed (Snakeskin® dialysis tubing, 3 kDa MWCO, ThermoFisher) against water (1:10,000 dilution ...
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bioRxiv - Bioengineering 2019Quote: ... TaqMan qPCR probes (Thermo Fisher Scientific, Supplementary Table 3) and Fast Advanced Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... in 3 ml of DMEM/F12 medium (Thermofisher Scientific). The pancreas tissue was further digested in DMEM/F12 supplemented with collagenase D (Sigma ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... and stained with TO-PRO-3 viability dye (ThermoFisher). Cells were then filtered through a 35 µm cell strainer (Corning ...
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bioRxiv - Microbiology 2019Quote: ... yeasts treated with DNAse (3 U/mL, Ambion®) were used as a TUNEL positive control.
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bioRxiv - Neuroscience 2019Quote: ... 1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate (DiI, Invitrogen, USA) was used as a non-viral neural tracer ...
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bioRxiv - Microbiology 2019Quote: Beads (ThermoFisher Cat R0300, 3 μm red fluorescent beads) were autoclaved in a factory-clean glass tube ...
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bioRxiv - Developmental Biology 2019Quote: ... Nuclei were labelled with TO-PRO-3 (Thermo Fisher). Images were captured on an LSM880 Examiner confocal system (Carl Zeiss Ltd ...
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bioRxiv - Molecular Biology 2019Quote: ... Nuclei were stained with ToPRO 3-Iodide (Molecular Probes). Images were obtained on a Leica SP8 confocal microscope.
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bioRxiv - Molecular Biology 2019Quote: ... and 3% phalloidin-AF488 (catalog #: A12379; Thermo Fisher Scientific) was placed on sections in the dark for 30 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... washed 3 times with DPBS (Gibco, cat#14190-136), then permeabilized with 0.25% Triton X-100 (Thermo Scientific ...
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bioRxiv - Biophysics 2021Quote: ... Mirius (1:3) in Opti-MEM media (Thermo scientific). Cell confluency was allowed to reach 70-80% prior to transfection ...
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bioRxiv - Neuroscience 2021Quote: ... and crystals of 3 kDa fluorescein dextran (D3306; Invitrogen) were applied to the proximal cut end of the chorda tympani ...
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bioRxiv - Genetics 2019Quote: ... SV40 3’UTR from pAc5.1-V5-HisB (Invitrogen®) terminated transcription of transgenes ...
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bioRxiv - Cell Biology 2020Quote: ... anti-aggrecan antibody BC-3 was from Thermo Scientific and anti-decorin antibody ab175404 was from Sigma.
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bioRxiv - Microbiology 2021Quote: ... using QuantStudio 3 Real-Time PCR System (Applied Biosystems) according to instructions of the manufacturer ...
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bioRxiv - Bioengineering 2021Quote: ... using a QuantStudio 3 PCR system (Thermo Fisher Scientific). Osteoblast ...
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bioRxiv - Bioengineering 2021Quote: ... and 3 minutes in xylene (Fisher Scientific X5-500). 1-2 drops of CytoSeal mounting media (Thermofisher ...
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bioRxiv - Bioengineering 2021Quote: ... or rabbit anti-caspase-3 (1:500, ThermoFisher #700182) followed by Alexa-conjugated anti-mouse or anti-rabbit secondary antibodies for 8 hours each at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... 3) latrunculin-b (Lat-B; 2 μM; Fisher Scientific), 4 ...
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bioRxiv - Biochemistry 2020Quote: ... N-cyclohexyl-3- aminopropanesulfonic (CAPS) was from Acros Organics, 2-(N-Morpholino)ethanesulfonic acid (MES ...
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bioRxiv - Biophysics 2021Quote: ... counted using a Countess 3 FL cell counter (Invitrogen), centrifuged for 2 min at 200 g ...