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Citations for Thermo Fisher :
6451 - 6500 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... were placed in a QuantStudio 3 (Thermofisher Scientific, cat.#A28567) operating the following program ...
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bioRxiv - Developmental Biology 2023Quote: ... A QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific) was used for quantitative real-time PCR experiments ...
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bioRxiv - Cell Biology 2023Quote: ... Rbl2#3 (AM16708 ID#68921) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... The reaction was performed in an QuantStudio 3 (Applied Biosystems). Data was obtained using the software QuantStudio Design and Analysis v1.4 (Applied Biosystems).
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bioRxiv - Molecular Biology 2023Quote: ... on a Quantstudio 3 Real-Time PCR cycler (ThermoFisher Scientific). See Supplementary Table S1 for primer sequences.
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bioRxiv - Cell Biology 2024Quote: ... and mixed with 3 µL of Lipofectamine RNAiMAX (ThermoFisher Scientific) transfection reagent prediluted in 47 µL Opti-MEM reduced serum medium plus GlutaMAX ...
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bioRxiv - Microbiology 2024Quote: ... qPCR reactions were performed on a QuantStudio 3 (Thermo Fisher) and analyzed using GraphPad Prism software ...
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bioRxiv - Neuroscience 2024Quote: ... with passaging every 2-3 days with trypsin/EDTA (Gibco). 3 biological replicates were generated for proteomic analysis ...
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bioRxiv - Neuroscience 2024Quote: ... 3 sodium pyruvate and 0.40 L-ascorbic acid (Acros Organics), bubbled with 5% CO2 in 95% O2 ...
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bioRxiv - Molecular Biology 2024Quote: ... and blocked with PBS containing 3% bovine serum albumin (ThermoFisher) and 5% normal goat serum (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... 3 sodium pyruvate and 0.40 L-ascorbic acid (Acros Organics), bubbled with 5% CO2 in 95% O2 ...
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bioRxiv - Cell Biology 2024Quote: ... washed 3 x 5 min with PBS (Gibco #14200-067) and fixed with 4 % (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... Protein was loaded on a 3-12% gel (Invitrogen BN10001B0X) and run using Invitrogen buffers (BN2002 ...
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bioRxiv - Immunology 2024Quote: ... SKOV-3 cells were maintained in McCoy’s 5A media (Gibco) supplemented with 10% HI FBS and 1X Anti-Anti ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 µL of Lipofectamine LTX (Thermo Fisher Scientific, Waltham, MA) was diluted in optiMEM ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were diluted (1:3) using loading buffer (ThermoFisher), heat-denatured for 3 minutes at 95°C ...
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bioRxiv - Biochemistry 2024Quote: ... and 3 μg/ml anti-HA antibody (Invitrogen, Cat# 26183) for one hour and a half at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... and counted using a Countess 3 Automated Cell Counter (ThermoFisher). All lines were determined to be mycoplasma free using MycoAlert Detection Kit (Lonza).
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bioRxiv - Immunology 2024Quote: ... on a QuantStudioTM 3 Real-Time PCR Instrument (Applied Biosystems). The following primers were used for qRT-PCR: Vcam1 forward primer 5’-AGTTGGGGATTCGGTTGTTCT-3’;Vcam1 reverse primer 5’-CCCCTCATTCCTTACCACCC-3’;C1qa forward primer 5’-AAAGGCAATCCAGGCAATATCA-3’;C1qa reverse primer 5’-TGGTTCTGGTATGGACTCTCC-3’;H2-eb1 forward primer 5’-GCGGAGAGTTGAGCCTACG-3’;H2-eb1 reverse primer 5’-CCAGGAGGTTGTGGTGTTCC-3’;Ccl8 forward primer 5’-TCTACGCAGTGCTTCTTTGCC-3’;Ccl8 reverse primer 5’-AAGGGGGATCTTCAGCTTTAGTA-3’;Il27 forward primer 5’-CTGTTGCTGCTACCCTTGCTT-3’;Il27 reverse primer 5’-CACTCCTGGCAATCGAGA-3’;Gapdh forward primer 5’-AGGTCGGTGTGAACGGATTTG-3’;Gapdh reverse primer 5’-TGTAGACCATGTAGTTGAGGTCA-3’.All data were normalized to Gapdh quantified in parallel amplification reactions.
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bioRxiv - Immunology 2024Quote: Doxazosin (#77883-43-3, Thermo Fisher Scientific, Houston, TX, USA) an α1 antagonist ...
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bioRxiv - Immunology 2024Quote: PCR was performed using the QuantStudio 3 system (Applied Biosystems), with Advanced qPCR Mastermix (Wisent ...
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bioRxiv - Immunology 2024Quote: ... Doxazosin (#77883-43-3, Thermo Fisher Scientific, Houston, TX, USA), and/or Atipamezole (Cayman Chemical ...
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bioRxiv - Cancer Biology 2024Quote: ... endogenous peroxidase was quenched in 3% hydrogen peroxide (Fisher Scientific) for 30 min at room temperature prior to antigen retrieval ...
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bioRxiv - Microbiology 2024Quote: ... 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine 4-chlorobenzenesulfonate salt (DiD; Invitrogen). IAV sizes were determined via dynamic light scattering (DLS ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by 3 washes in DPBS (Thermo Scientific, Waltham, MA) supplemented with 5% fetal bovine serum ...
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bioRxiv - Immunology 2022Quote: ... and suspended in Fc Block for 5 min at RT prior to staining with a surface stain of following antibodies: CD3 (Invitrogen, 45-0031-82)/CD4 (BD ...
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bioRxiv - Molecular Biology 2021Quote: ... and their derivatives were cultured in DMEM containing 1 g/l glucose supplemented with 10% heat-inactivated FCS (Fetal Calf Serum, Gibco, Cat#: 10270-106), 2 mM L-glutamine (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: ... 20 minutes in 30 mL mucus dissociation solution (5 mM dithiothretol [DTT; Invitrogen 15508] and 2% heat-inactivated fetal calf serum [FCS; Gibco A38401] in HBSS); 25 minutes in 30 mL epithelial removal solution (5 mM EDTA and 2% FCS in HBSS) ...
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bioRxiv - Systems Biology 2020Quote: ... Fragments were rocked on ice with crypt isolation buffer (10 mM EDTA, Life Technologies, in PBS0 supplemented with 500X dilution of Primocin, Invivogen, 10 mM HEPES, Life Technologies, and 2% FCS, Life Technologies) for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... The concentration of glucose in filtrate was quantified using Glucose CII-Test Wako (FUJIFILM Wako Pure Chemical Corporation) by measuring the absorbance at 492 nm with a Thermo Scientific Multiscan® FC (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2020Quote: ... The absorbance at 340 nm was read every 12 s at 37°C using a Multiskan FC plate-reader (Thermo Scientific, Paisley, UK). Maximum absorbance ...
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bioRxiv - Microbiology 2020Quote: ... Then the resultant supernatants were transferred to a 96-well plate for the absorbance measurement at 492 nm with a microplate photometer (Multiskan FC, Thermo Fisher Scientific, USA). RBCs treated with phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2021Quote: ... Planktonic cell growth was determined by quantifying optical densities at 595 nm using a microplate spectrophotometer (Thermo scientific multiscan FC: Cat no. 51119000). Plates were thoroughly washed three times with 200 µl of sterile PBS per well using a microplate washer (Micro wash 1100 from Global diagnostics ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended at a concentration of 1 × 106 cells/mL in RPMI without FCS and labeled with CellTracker™ Blue CMAC dye (10 μM, Thermo Fisher, C2110) for 20 min at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: Collected cells were washed and resuspended in staining buffer (PBS 1X +1% BSA) with Fc Receptor Binding Inhibitor Antibody (Invitrogen, # 14-9161-73) for 15 minutes at 4°C ...
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bioRxiv - Cell Biology 2022Quote: RPE-1 cells were maintained in DMEM media supplemented with FCS (10 % final concentration) and Penicillin Streptavidin-Glutamine (PSG; Gibco, 1 % final concentration). For starvation ...
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bioRxiv - Neuroscience 2021Quote: ... cells were collected from the flask, centrifuged (10 min, 150g, RT) and blocked for Fc receptors by using antibody CD16/32 (Invitrogen, 14-0161-82) (1:100) ...
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bioRxiv - Neuroscience 2021Quote: ... Secreted dimeric C-terminally Fc-tagged MDGA1 stably expressed in HEK293T cells was collected in serum-free Opti-MEM (Thermo Fisher Scientific, Inc.). Fc-tagged MDGA1 protein was run on an affinity column packed with Protein-G Plus Agarose fast flow resin (Pierce ...
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bioRxiv - Molecular Biology 2021Quote: ... using DMEM (4.5 g/l glucose) with 2 mM Glutamax-I medium supplemented with 15% foetal calf serum (FCS) (ThermoFisher Scientific, Cat# 10270-106), 0.1% β-mercaptoethanol (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... were resuspended in 100 μL of FC buffer containing primary antibodies (mouse anti-Spike S2, Thermo Fisher, Cat #: MA5-35946, 1:500 dilution) (mouse anti-Flag M2 ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 5% fetal calf serum (FCS) (Eurobio) and 100 U/ml penicillin and 100 μg/ml streptomycin (both from Gibco BRL Life Technologies). Cells were maintained at 37°C in the presence of 5% CO2.
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bioRxiv - Immunology 2022Quote: ... were cultured in R10 medium (RPMI1640 [Gibco Life Technologies, 31870-025], 10% FCS, 2 mM glutamate [Gibco Life Technologies, 25030-024], 1 mM sodium pyruvate [Gibco Life Technologies, 11360-039] ...
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bioRxiv - Neuroscience 2024Quote: ... Media was removed and cells were subsequently incubated in 50nM Aβ-containing complete DMEM media (10% FCS (Fisher Scientific, Cat#: 10-438-026), 1% P/S (Gibco ...
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bioRxiv - Genomics 2024Quote: ... tissue-culture plates in DMEM (4.5 g/L glucose) with GlutaMAX-I supplemented with ES-tested 15% foetal calf serum (FCS, ThermoFisher Scientific, Cat# 10270-106), 0.1% β-mercaptoethanol (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... The absorbance reading was conducted at 595 nm using a microplate photometer (IVD model, MultiskanTM FC, Thermo Fisher Scientific Inc., MA, United States).
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bioRxiv - Microbiology 2023Quote: ... Hungary) substrate and the optical density of the solution was measured at 450 nm using a Multiscan FC reader (Thermo Fisher Scientific Inc.). Blood samples were centrifuged after collection and the sera were kept at −70°C ...
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bioRxiv - Cell Biology 2023Quote: ... using either a WPA CO8000 Cell Density Meter (Biochrom, Cambridge, UK) or a Multiskan FC microplate reader with a 594 nm filter (Thermofisher, Waltham, MA, USA).
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bioRxiv - Cell Biology 2022Quote: ... The single cell suspension was incubated with 1:1000 Fc Block (AbLab) in 1X FACS Buffer and stained for surface markers before being fixed/permeabilized (Thermofisher 00-5523-00) for FoxP3 staining (invitrogen 11-5773-82 Table 2).
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bioRxiv - Cell Biology 2023Quote: ... plus 10 % FCS (PAA Laboratories; Pasching, Austria) and P/S (100 U/ml penicillin and 100 mg/ml streptomycin; Gibco, Thermo Fisher Scientific). Results with HeLa K are shown in Fig ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... centrifuged for 5’ on 1.4 rpm and were incubated with Fc Blocker (1:100, in 1XPBS + 1%FBS, Thermo Fisher Scientific, Ghent, Belgium) for 30’ on ice ...