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Citations for Thermo Fisher :
6051 - 6100 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Goat anti-human IgG AP conjugate and PNPP Substrate tablets (ThermoFisher) were used to detect binding ...
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bioRxiv - Bioengineering 2023Quote: ... Human Embryonic Kidney cells (HEK293-CD19+) were cultured in DMEM (Gibco), 10% heat-inactivated FBS (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: The MB dataset GSE85217 (Affymetrix Human Gene 1.1 ST Array, GPL22286) contains 763 gene expression samples and was acquired from Gene Expression Omnibus (GEO ...
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bioRxiv - Cancer Biology 2023Quote: ... Human embryonic kidney cell line HEK293FT was obtained from Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... VEGF ligand (recombinant human protein) was ordered from ThermoFisher (CAT: #PHC9391).
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bioRxiv - Molecular Biology 2023Quote: ... and 5 ng/uL human IL-6 recombinant protein (ThermoFisher PHC0066).
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bioRxiv - Systems Biology 2023Quote: Different human cell lines (HeLa) were cultured in DMEM (Gibco, Invitrogen), supplemented with 10% fetal bovine serum ...
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bioRxiv - Systems Biology 2023Quote: Different human cell lines (HeLa) were cultured in DMEM (Gibco, Invitrogen), supplemented with 10% fetal bovine serum ...
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bioRxiv - Molecular Biology 2023Quote: ... and activated with Dynabeads Human T Activator CD3/CD28 (Gibco, USA) at a 1:1 ratio for 24 hours ...
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bioRxiv - Immunology 2023Quote: Adipocytes were differentiated from human adipose-derived stem cells (ADSCs, ThermoFisher), which were obtained from human lipoaspirate tissue and cryopreserved from primary culture ...
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bioRxiv - Immunology 2023Quote: ... were obtained from ThermoFisher and maintained in Human Large Vessel Endothelial Cell Basal Medium (ThermoFisher) according to manufacturer’s protocol.
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Chimeric Antigen Cytotoxic Receptors for In-Vivo Engineering of Tumor-targeting Natural Killer CellsbioRxiv - Immunology 2023Quote: ... Cytokine & Chemokine Convenience 34-Plex Human ProcartaPlex™ Panel 1A (Invitrogen) was used to collect and analyze the supernatant for levels of cytokines/chemokines following the protocol provided by the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... CCL5 and CCL2 (Human cytokine magnetic 25-plex panel; Life technologies).
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bioRxiv - Immunology 2023Quote: ... and erythrocytes using the Dynabeads Untouched Human Monocytes Kit (Invitrogen, 11350D) (Supplemental Figure 2).
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bioRxiv - Developmental Biology 2023Quote: ... KiPS line was derived by reprogramming of commercial human keratinocytes (Invitrogen) with Sendai viruses encoding for OSKM 62 and kindly provided by Austin Smith’s laboratory ...
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bioRxiv - Cell Biology 2023Quote: Human embryonic kidney HEK293T cells were maintained in DMEM/F12 (Gibco) with 10 % FCS (Gibco ...
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bioRxiv - Genomics 2023Quote: Commercially available human iPSC lines derived from a female donor (Gibco Episomal hiPSC ...
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bioRxiv - Cancer Biology 2024Quote: ... added with human complete organoid medium containing Advanced DMEM/F12 (GIBCO), 10 mM HEPES (GIBCO) ...
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bioRxiv - Molecular Biology 2024Quote: Human MET knockout HeLa cells were transiently transfected with Lipofectamine3000 (Invitrogen) according to the manufacturer’s protocols in a 6-well plate format ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 ng/ml human basic FGF recombinant protein (bFGF) (Gibco, 13256029), 2% B27 supplement (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 20 ng/ml human EGF recombinant protein (Gibco, PHG0314), 20 ng/ml human basic FGF recombinant protein (bFGF ...
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bioRxiv - Neuroscience 2024Quote: ... miBrains were cultured in Human Endothelial Serum-Free Medium (ThermoFisher Scientific) with 1% pen/strep (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: The following human (h) and rat (r) TaqMan assays (Thermo Fisher) were used ...
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bioRxiv - Neuroscience 2024Quote: ... Rabbit anti-Human IgG DyLight™ 800 (Cat# SA5-10116, Thermofisher). For the appraisal of common painkillers drugs were as follows ...
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bioRxiv - Cell Biology 2024Quote: ... and 2.5 ng/mL recombinant human hepatocyte growth factor (Gibco, UK).
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bioRxiv - Immunology 2024Quote: ... and human IL-6 (Gibco, PeproTech, #200-06; 20 ng/mL). Prostaglandin E2 (PGE2 ...
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bioRxiv - Immunology 2024Quote: ... and cytokine levels were measured using Human Uncoated ELISA Kits (ThermoFisher) targeting IFNψ ...
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bioRxiv - Developmental Biology 2024Quote: ... Complete human medium with 10% exosome depleted FBS (Gibco, Massachusetts, US) was replenished and device placed into incubator ...
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bioRxiv - Cell Biology 2024Quote: ... Human Pools Set v3.0 kit (Applied BiosystemsTM, Thermo Fisher Scientific, USA) and the TaqManTM MicroRNA Reverse Transcription Kit (Applied BiosystemsTM ...
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bioRxiv - Systems Biology 2024Quote: ... Human THP-1 monocytes were cultured in RPMI 1640 medium (Gibco) supplemented with 10% FBS ...
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Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progressionbioRxiv - Cancer Biology 2024Quote: ... Normal human epithelial cells were cultured in keratinocyte-SFM medium (Gibco) supplemented with 1% antibiotic-antimycotic (Gibco).
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bioRxiv - Genomics 2024Quote: We used the human Amyloid-β 40 (ThermoFisher Cat No. KHB3481), Amyloid-β 42 (ThermoFisher Cat No ...
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bioRxiv - Immunology 2024Quote: ... stimulated with Dynabeads™ Human T-Activator CD3/CD28 (Gibco™) and cultured for 5 days in AIMV medium supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Biochemistry 2020Quote: ... The gene optimized for the Drosophila codon usage and extended by C-terminal His-tag was purchased from Life Technologies (DNA String fragments, USA). The DNA fragment was inserted into a pMT-BiP-V5-His_A vector (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: 1-2 μg of purified 6xHis-tagged calcineurin or 6xHis-MBP as a negative control was first bound to 10 μL magnetic Dynabeads™ His-Tag Isolation and Pulldown Beads (Thermo Fisher Sci., 10104D) in 490 μL base buffer (50 mM Tris-HCl pH 7.5,150 mM NaCl ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by sequencing using the Ion PI™ Hi-Q™ Sequencing 200 Kit on Ion Proton PI™ V2 chips (Thermo Fisher Scientific), an Ion Proton™ System ...
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bioRxiv - Genetics 2020Quote: ... in all the above clonings except for the his-72::GFP repair template which required ccdB resistant bacteria for which we used One Shot ccdB Survival (Thermo Fisher Scientific, cat. A10460). DNA extractions by miniprep were done with the ZymoPURE Plasmid Miniprep kit and elution with water (Zymo Research ...
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bioRxiv - Immunology 2020Quote: ... Nunc MaxiSorp ELISA plates were coated with 50 μL of capture antibody (anti-6x-His antibody clone HIS.H8; 2 μg/mL; Thermo Fisher Scientific, Waltham, MA, USA) in phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2024Quote: ... BMDMs were plated a day in advance on a coverslip lined 24-well plate at 8 x105 cells in 1mL per well, in “BMDM media” supplemented with 10% L929 (20% HI FBS, 1% penicillin-streptomycin, 1% non-essential amino acids (ThermoFisher, cat#11-140-050), 1% sodium pyruvate) ...
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bioRxiv - Microbiology 2024Quote: ... The amplified PCR product of the C-His oipA gene fragment was cloned into vector pLATE11 using aLICator LIC Cloning and Expression Kit 1 (Thermo Fisher Scientific, United States) by following the kit’s instructions ...
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bioRxiv - Pathology 2023Quote: ... The cDNA was used for RT-qPCR with qPCRBIO SyGreen Blue mix Hi-ROX (PCR Biosystems, London, UK) on the StepOne Plus Real-Time PCR machine (Applied Biosystems, Foster City, CA) and primers listed in Table S2 ...
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bioRxiv - Microbiology 2023Quote: ... Protein bands were visualized with Coomassie staining (ROTIBlue Quick, Carl Roth) or immunodetected with a monoclonal anti-His antibody conjugated to HRP (MA1-21315-HRP, Thermo Fisher, 1:2000 diluted). A detailed protocol can be found in protocols.io (dx.doi.org/10.17504/protocols.io.bu67nzhn).
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bioRxiv - Genomics 2023Quote: ... Vero/hSLAM and VeroE6/TMPRSS2 were maintained in supplemented DMEM with 10% HI-FBS and 1 mg/mL G418 sulfate (Thermo Fisher 10-131-027). HeLa/ACE2/TMPRSS2 cells were a generous gift from Dr ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% (v/v) heat-inactivated (HI) FBS (Thermo Fisher Scientific, Waltham, MA, USA). Stable cell lines expressing F-CIC-S ...
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bioRxiv - Immunology 2021Quote: ... Mice were genotyped by PCR using forward primers 5’-ctgagcagagacccactgaaag-3’ and reverse primers 5’- ggatctggcttctgagtttgtgta-3’ and amplicons were ran in 6% TBE gels (Life Technologies, Carlsbad, CA).
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bioRxiv - Molecular Biology 2021Quote: ... The probe was biotin labeled at the 3’ end using a Pierce™ Biotin 3’ End DNA Labeling Kit (Thermo Fisher Scientific Inc.). Seven micrograms of nuclear protein were added to a binding reaction mixture containing 2µl 10X binding buffer ...
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bioRxiv - Biochemistry 2021Quote: ... We did this by amplification of the GlyR-FP plasmids using PCR with primers 5’-ATATGGTACCTGGGAGGTCTATATAAGCAGAG-3’ and 5’ATAAGGTACCCCAGGCGGGCCATTTACCGTA-3’ followed by digestion with KpnI (ThermoFisher Scientific, Merelbeke, Belgium) and ligation using instant sticky-end ligase Master mix (NEB ...
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bioRxiv - Microbiology 2020Quote: Vero E6 and Calu-3 cells (Calu-3:ATCC HTB-55; Vero E6: ATCC, CRL-1586) were maintained in high glucose DMEM (Gibco, Waltham, MA, USA) supplemented with 10% FBS (R&D Systems ...
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bioRxiv - Cancer Biology 2020Quote: Apoptosis was measured by caspase-3/7 staining using CellEvent® Caspase-3/7 Green ReadyProbes® Reagent (ThermoFisher Scientific Inc., cat# R37111). CHLA20 and NGP cells were grown until confluence on 6-well plates with six days of treatment with DMSO or GSK591 ...
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bioRxiv - Cell Biology 2020Quote: ... 16nM TIMM23 (5’ CCCUCUGUCUCCUUAUUUA 3’, Eurogentech) or 16nM TIM22 (5’ GUGAGGAGCAGAAGAUGAU 3’, Eurogentech) using the Invitrogen Lipofectamine RNAiMAX Reagent (Invitrogen, Carlsbad, CA, USA) diluted with serum-free Gibco Opti-MEM I medium (Gibco ...