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Citations for Thermo Fisher :
6051 - 6100 of 10000+ citations for 7 METHOXY 4 4 DIMETHYL 3 4 DIHYDRO 2H NAPHTHALEN 1 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 30 µg of TDP-43 protein extract or 50µg of RGNEF extract was then resuspended in 1 X NuPAGE LDS Sample buffer (4 X) (Thermo Fisher Scientific) and denaturated at 85°C for 5 min ...
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bioRxiv - Bioengineering 2023Quote: ... samples were incubated overnight at 4 °C with secondary antibody Alexa 647 donkey-α-rabbit (1:500 in blocking solution; Invitrogen), Alexa 568 donkey-α-mouse (1:500 in blocking solution ...
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bioRxiv - Immunology 2023Quote: ... The samples were then stained with the blood lineage antibody panel (Table 1) for 30 minutes at 4°C and then washed with PBS (ThermoFisher Scientific) and 2% (v/v ...
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bioRxiv - Immunology 2023Quote: ... was incubated overnight at 4°C and Alexa Fluor 488 goat anti-chicken IgY (H+L) secondary antibody (1:100, A-11039, ThermoFisher Scientific) was applied to detect GFP+ cells ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed with PBS 15 mins × 4 in a slide mailer and incubated in secondary antibody at 1:1000 dilution (goat anti-chicken 647, Themo Fisher Scientific Cat# A-21449 and goat anti-rabbit 546 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then washed with PBS with 0.03% NaN3 for 15 mins RT × 4 and incubated in secondary antibodies at 1:1000 dilution (goat anti-mouse 488, Thermo Fisher Scientific# A-28175 ...
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bioRxiv - Microbiology 2023Quote: ... sections were counterstained with 1 µg/ml of 4’,6-diamidino-2-phenylindole (DAPI, #D1306, Thermo Fisher Scientific, Waltham, MA, USA) then ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then rinsed three times for 10 min in PBS and incubated for 24 h at 4°C in secondary antibodies (donkey anti-rabbit Alexa Fluor 488, 1:250, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated in the following primary antibodies overnight at 4°C: rabbit-anti-ZO1 (40-2200, Thermo Fisher Scientific, 1:200) and mouse-anti-RPE65 (MAB5428 ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated O/N at 4°C with gentle agitation with 1:2000 goat anti-human complement C3 antibody (Thermo Scientific) diluted in 1X TBS/0,5% Tween/3% BSA ...
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bioRxiv - Cell Biology 2024Quote: ... Part of the supernatant was conserved as total lysate and the remainder was subjected first to a preclearing step with agarose for 1 h at 4°C and then to pull-down assay with NeutrAvidin agarose (Thermo Scientific) for 2 h at 4°C to isolate biotinylated proteins.
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bioRxiv - Developmental Biology 2024Quote: ... incubated for 30 minutes in a live/dead Viability/Cytotoxicity solution containing 4 mM Calcein and 8 mM Ethidium homodimer-1 (L3224, ThermoFisher Scientific) at 37 °C ...
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bioRxiv - Pathology 2024Quote: ... pH 7.4 and 500 mM NaCl, 20 mM imidazole, 1 mM PMSF, protease inhibitor cocktail Set III, EDTA free from Thermo Fisher) and lysed by an LM20 microfluidizer (15000 psi) ...
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bioRxiv - Cell Biology 2024Quote: ... overnight at 4°C followed by incubation for 1 hour with secondary goat anti-mouse IRDye 800 (Thermo Fisher Scientific, MA) or goat anti–rabbit Alexa Fluor 680 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were washed with 5 mL PBS (4°C) and trypsinized with 1 mL 0.25% Trypsin-EDTA per dish (Gibco, 25200-056) for 5 min at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2024Quote: ... A172 cell line was cultured in high glucose DMEM media plus 4 mM L-glutamate and 1 mM sodium pyruvate (Thermo Fisher) supplemented with 10% FBS and 1% penicillin and streptomycin mixture ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were subsequently permeabilized with 0.5% Triton solution for 5 min at 4°C and then incubated with PicoGreen (1:1000 dilution) (Cat No: P11496, ThermoFisher Scientific) in PBST buffer (PBS plus 0.1% Tween-20 ...
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bioRxiv - Molecular Biology 2024Quote: ... lysed and sonicated using Diagenode Bioruptor® Pico sonicator for 10 cycles with alternating 30 secs on/ off at low intensity and 1 mg of protein was digested with 4 U of Turbo DNase (Ambion, AM2238) and 0.28 U/ml (low ...
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bioRxiv - Neuroscience 2024Quote: ... Then the sections were incubated with primary antibodies in blocking buffer overnight at 4°C: AT8 (Mouse, 1:100, Thermo Scientific), and Iba1 (Rabbit ...
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bioRxiv - Neuroscience 2024Quote: ... Adult flies were fixed with 4% paraformaldehyde in 1X PBS for 1 hour after a quick dissection,then immersed in OCT compound (Fisher Scientific) and flash frozen in liquid nitrogen or dry ice ...
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bioRxiv - Immunology 2024Quote: ... the slices were incubated for 24 hours at 4°C with Alexa Fluor 488 secondary antibody (1:750, Thermo Fisher Scientific). Finally ...
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bioRxiv - Biochemistry 2024Quote: A total of 1 μg of plasmid DNA (TLR3:minibinder-protein A ratio of 1:4) was transfected into 2.5×106 Expi 293F cells (#A14527, Thermo Fisher Scientific) using Expifectamine (#A14524 ...
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bioRxiv - Biochemistry 2024Quote: ... Excess sample was removed with a blotting time of 4 s and a blotting force of 1 at 4 °C prior to plunge freezing into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). A total of 9,018 movies were recorded with a K3 detector (Gatan ...
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bioRxiv - Biochemistry 2024Quote: ... Excess sample was removed with a blotting time of 4 s and a blotting force of 1 at 4 °C prior to plunge freezing into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). A total of 8,294 movies were recorded with a K3 detector (Gatan ...
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bioRxiv - Developmental Biology 2024Quote: ... The fixed ovaries were washed in washing buffer (WB, 1% BSA in 1X PBS) thrice before being embedded into 4% low-melting point agarose (Thermo Fisher). The embedded tissue was sliced into 100 µm thick tissue sections using a vibratome (Leica ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were then washed and incubated overnight at 4°C with secondary antibodies Goat-anti-Chicken 488 (1:400, Thermo Fisher Scientific Cat# A-11039 ...
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bioRxiv - Developmental Biology 2024Quote: ... for one hour at RT and stained overnight at 4 °C with primary antibody Rabbit anti-GFP diluted 1:500 (#A11122 Thermo Fisher). Samples were washed three times using 0.3% PBT and stained with secondary antibody Alexa Fluor 488 Goat anti-Rabbit diluted 1:1000 (#R37116 ...
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bioRxiv - Neuroscience 2024Quote: ... Neurobiotin-injected specimens for the tracer-coupling analysis of HCs were first incubated overnight at 4°C with Alexa568-conjugated streptavidin (1:250, Invitrogen, S11226), diluted in incubation buffer containing 10% NDS ...
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bioRxiv - Microbiology 2024Quote: ... Beads were washed three times with 500 µl of IP buffer (500 x g, 1 min, 4°C) and resuspended in 65 µl of NuPAGE LDS-Sample buffer (Thermo Fisher) supplemented with 100 mM DTT ...
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bioRxiv - Immunology 2024Quote: ... CAL-1 cells were infected with Salmonella for 4 h and then incubated for 30 min at 37 °C with CellROX Deep Red Reagent (1 µM, #C10422 Invitrogen Thermofisher) for total (cytoplasmic and nuclear ...
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bioRxiv - Molecular Biology 2024Quote: ... Fractions 1-4 were pooled and concentrated using centrifugal filters units (30 kDa MWCO, MilliporeSigma Amicon Ultra Centrifugal Filter Unit, Fisher Scientific) at 4000 × g for 30 min to a final volume of 100uL.
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bioRxiv - Physiology 2024Quote: Intracellular Ca2+ [Ca2+]i measurements were performed in cardiomyocytes loaded with Fluo-4 AM dye (5 μmol.L-1, ThermoFisher, Cat #F14201). After a 30-minute loading period ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were incubated overnight at 4°C with an anti-HIV-1 p24 monoclonal (AG3.0) primary antibody (4 µl/mL) (NIH HIV Reagent Program catalog# 4121) or P2RY12 primary antibody (1:100, 702516, Thermo Fisher) or TMEM119 primary antibody (1:50 ...
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bioRxiv - Biochemistry 2024Quote: ... Excess sample was removed with a blotting time of 4 s and a blotting force of 1 at 4 °C prior to plunge freezing into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). A total of 6,812 movies were recorded with a K3 detector (Gatan ...
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bioRxiv - Biochemistry 2024Quote: ... Excess sample was removed with a blotting time of 4 s and a blotting force of 1 at 4 °C prior to plunge freezing into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). A total of 6,650 movies were recorded with a K3 detector (Gatan ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were incubated with 20-fold molar excess of either IANBD [N,N’-dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol)ethylenediamine or Alexa Fluor 546 (AF546) maleimide (Molecular Probes] for 2 h at RT ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were incubated with 20-fold molar excess of either IANBD [N,N ′ -dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol) ethylenediamine or Alexa Fluor 546 (AF546) maleimide (Molecular Probes] for 2 h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... was dissolved in 1 mL dimethyl sulfoxide (DMSO, Fisher Scientific) and adjusted to a final concentration of 0.5 mg/ml CNO in 7.5% v/v DMSO in sterile saline ...
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bioRxiv - Biophysics 2021Quote: ... supernatants were analyzed on a 4-20% gradient tris-glycine SDS-PAGE gel (Thermo Fisher). Gels were imaged to visualize the GFP signal ...
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bioRxiv - Biochemistry 2020Quote: ... cell lysates were loaded onto 4%-12% or 12% Bis-Tris gels (NuPAGE, Life Technologies) along with a Precision Plus protein dual-color standard (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: ... sections were fixed in ∼100ul of 4% paraformaldehyde (PFA; Thermo Fisher Scientific, Rockford, IL, USA) at room temperature for 15 minutes and then washed 2 times for 5 minutes each ...
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bioRxiv - Cancer Biology 2021Quote: The samples were separated by SDS-PAGE (NuPAGE 4-12% Bis-Tris Protein Gels, Thermofisher), followed by transfer to nitrocellulose membrane via wet transfer at 90 V for 2–2.5 hours ...
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bioRxiv - Developmental Biology 2020Quote: ... After 48 hours the surviving GV oocytes were either fixed in 4% paraformaldehyde (Thermo Scientific) for investigation by IF ...
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bioRxiv - Biochemistry 2021Quote: Co-IP samples were loaded and separated on a 4-20% Tris-Glycine gel (Invitrogen). Proteins were stained with colloidal Coomassie Brilliant Blue G-250 ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 ug of small RNA purified by the mirVana miRNA Isolation kit (Thermo Fisher Scientific) was heated at 90 °C for 20 sec with gel loading buffer II (Thermo Fisher Scientific ...
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Receptor tyrosine kinases regulate signal transduction through a liquid–liquid phase separated statebioRxiv - Cell Biology 2019Quote: The calcium assay was perform using Fluo-4 NW Calcium Assay Kit (F36206, ThermoFisher Scientific) according to the manufactural manual ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated by electrophoresis on Bolt™ 4-12% Bis-Tris Plus Gels (Invitrogen). Proteins were transferred onto nitrocellulose membranes using iBlot gel transfer stacks (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were fixed in 4% formaldehyde and analyzed by immunohistochemistry for S100B (ThermoFisher, Waltham, MA) and GFAP expression (Abcam ...
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bioRxiv - Cell Biology 2020Quote: ... the proteins were separated with the NuPAGE system (ThermoFisher, 4-12% Bis-Tris protein gel), and transferred to PVDF membrane (ThermoFisher) ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were subjected to electrophoresis on 4-12% NuPAGE Bis–Tris gels (Thermo Fisher, NP0335BOX) and proteins were transferred to nitrocellulose membranes using wet-based transfer system (Bio-Rad) ...