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Citations for Thermo Fisher :
6001 - 6050 of 10000+ citations for 7 METHOXY 4 4 DIMETHYL 3 4 DIHYDRO 2H NAPHTHALEN 1 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The samples were dissolved in 10 µL of reconstitution buffer (96:4 water: acetonitrile, 1% formic acid and analyzed by LC-MS/MS using QExactive (Thermo Fisher) in the proteomics core facility at EMBL Heidelberg (https://www.embl.org/groups/proteomics/).
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bioRxiv - Developmental Biology 2024Quote: ... embryos were incubated overnight at 4°C with the appropriate secondary antibody (Alexa Fluor 488 or 555 at 1/1000, Molecular Probes).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Primary antibodies were incubated overnight at 4 °C before washing and staining with goat anti-rabbit AlexaFluor647 (1:500, Invitrogen A21235) and goat anti-human AlexaFluor488 (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... The following day sections were rinsed three times in PBTriton 0.5% and incubated overnight at 4°C in secondary antibody solution using Goat anti-Rabbit-Oregon Green 488 (1:500 Invitrogen, 011038). Next ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were then washed with 0.1 M PBS (6×20 minutes at 4°C) and incubated with secondary antibodies conjugated to Cy3 and Alexa Fluor 488 (1:500, ThermoFisher, Germany) in 0.1 M PBS overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were resuspended in 1 ml of staining buffer and 4 drops (164 μl) of Propidium Iodide Ready Flow (Invitrogen, R37169) was added to the cells and incubated at 25 °C for 20 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 3 x 10 min with PBS and incubated over-night at +4°C with goat anti-rabbit Alexa Fluor 564 (1:1000, Invitrogen, ThermoFisher Scientific) and goat anti-mouse Alexa Fluor 488 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were rinsed 3x 10 min with PBS and incubated either for 2 hrs at RT or 24-72 hrs at 4° C in goat anti-rabbit Alexa Fluor 488 (1:1000, Invitrogen, ThermoFisher Scientific) secondary antibody in carrier solution containing 1% NGS ...
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bioRxiv - Neuroscience 2024Quote: ... Then the sections were incubated with primary antibodies in blocking buffer overnight at 4 °C: AT8 (THERMO SCIENTIFIC, MOUSE,1:100), IBA1(WAKO ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then rinsed three times for 10 min in PBS and incubated for 24 h at 4°C in secondary antibodies (donkey anti-rabbit Alexa Fluor 488, 1:250, Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: Cell viability was assessed following shear stress application by incubating each sample in a solution containing 2 µM calcein AM and 4 µM ethidium homodimer-1 in PBS (Live/Dead Viability/Cytotoxicity Kit; Thermo Fisher) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were split when they reached 80% confluency (every 2-4 days) using 0.5 mM EDTA in 1× DPBS (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: ... The samples were dissolved in 10 µL of reconstitution buffer (96:4 water: acetonitrile, 1% formic acid and analyzed by LC-MS/MS using QExactive (Thermo Fisher) in the proteomics core facility at EMBL Heidelberg (https://www.embl.org/groups/proteomics/) ...
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bioRxiv - Immunology 2023Quote: ... at 1:100 overnight at 4°C in 5% BSA in the presence of 0.05% sodium azide (BP922I-500; Thermo Fisher Scientific), 0.5% Triton X-100 (161-0407 ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with goat anti-rabbit IgG Alexa 488 secondary antibody (1:500 for 2 hours at 4°C; Invitrogen by ThermoFisher Scientific) in solution with PBS ...
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bioRxiv - Cell Biology 2023Quote: ... hTERT immortalized BJ-5ta foreskin fibroblasts were cultured in a 4:1 mixture of DMEM medium and Medium 199 (Gibco, Life Technologies) supplemented with 10% FBS ...
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bioRxiv - Biochemistry 2023Quote: ... Excess sample was removed with a blotting time of 3 s and a blotting force of 1 at 4 °C prior to plunge freezing into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). A total of 8,294 movies were recorded with a K3 detector (Gatan ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were washed five times with the same blocking solution and then incubated with the corresponding secondary antibodies conjugated with Alexa fluorophores at 4°C with gentle agitation for 24 h (1:1000, Molecular Probes). Finally ...
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bioRxiv - Immunology 2023Quote: ... ROR2 (4-1) (heavy and light chains connected by human IgG3 upper+middle Hinge) were synthesized by GeneArt (ThermoFisher, Regensburg, Germany) and subcloned into lentiviral backbones containing IgG3- or CD8α-derived spacer domains ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated for 2 hours with secondary antibody AlexaFluor 488 Goat Anti-Rabbit IgG (1:1000 in 4% BSA, Invitrogen #A11008) at RT in the dark ...
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bioRxiv - Biophysics 2023Quote: ... The clarified supernatant was rolled at 4°C with HisPur Ni-NTA Resin (1 mL resin per liter of cells, Thermo Scientific) for 2 hours ...
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bioRxiv - Biophysics 2023Quote: ... The clarified supernatant was rolled at 4°C with HisPur Ni-NTA Resin (1 mL resin per liter of cells, Thermo Scientific) for 2 hours ...
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bioRxiv - Bioengineering 2023Quote: ... To minimize nonspecific binding the samples were blocked for 4 h at room temperature with a blocking buffer containing 1% of Tween20 (Fisher Scientific) and 3 % of BSA (VWR ...
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bioRxiv - Molecular Biology 2023Quote: ... The immunoprecipitated proteins were run on 4-12 % SDS-PAGE gel by 1 cm from the well and stained with SimplyBlue (Thermo Fisher) for in-gel digestion ...
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bioRxiv - Immunology 2023Quote: ... The WIV was then dialyzed against 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)-buffered saline (Thermo Fisher Scientific, Bleiswijk, the Netherlands) at 4°C overnight to remove β-propiolactone ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatants of lysed cells were incubated in shake tubes over 1 h at 4°C with Pierce™ Protein G magnetic beads (Invitrogen) previously attached to V5 tag antibodies (R960-25) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated overnight at 4°C with a primary antibody directed against the V5-tag (ThermoFisher, 46-0705, 1:200) or against small double-stranded RNAs (J2 antibody ...
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bioRxiv - Biophysics 2023Quote: Fluorophores were prepared for linking into the gel by incubating them at 4°C overnight in 250 μl of PBS and 1% Acryloyl-X (w/v; Catalogue number A20770, Fisher Scientific).
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bioRxiv - Bioengineering 2023Quote: ... tissues were washed and stained for 48 h at 4°C with a 1:100 dilution of goat anti-mouse secondary antibody conjugated to Alexa-Fluor 488 (A28175, Life Technologies), goat anti-rabbit secondary antibody conjugated to Alexa-Fluor 555 (A21428 ...
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bioRxiv - Cell Biology 2023Quote: Human nasal brushings were collected fresh for this study and immediately placed in a 15-ml sterile Falcon tube containing 4 ml of transport medium (αMEM supplemented with 1× penicillin–streptomycin (Gibco, 15070), 10 ng/ml gentamicin (Gibco ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The following day embryos were thoroughly washed with NPBS + 0.1% Triton and incubated first in blocking solution for 3 hours and then overnight at 4°C in the dark in blocking solution with DAPI (1:500) and secondary antibodies: goat anti-mouse (Invitrogen 84540) and goat anti-rabbit (Abcam 150083) ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were incubated with primary antibody overnight at 4°C and then incubated with Alexa Fluor-conjugated donkey secondary antibodies (1:400, Molecular Probes) at RT for 2 hrs and then mounted in VECTASHIELD medium with 4′-6-Diamidino-2-phenylindole (DAPI ...
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bioRxiv - Molecular Biology 2023Quote: ... was equilibrated with lysis buffer and then incubated with the cell lysate at 4 °C for 1 h on an end-to-end rotator (Cat# 88881001, ThermoFisher scientific). The slurry was transferred to a 15 ml falcon tube ...
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bioRxiv - Immunology 2023Quote: Splenic B cells were resuspended at a concentration of 5 × 106 cell/ml in RPMI supplemented with 20 mM HEPES and 2.5% FCS and loaded with 1 μM Fluo-4 (Thermo Fisher, #F14201) and 3 μM Fura Red (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... were mixed in a 3:1 with sample buffer (NuPAGE™ LDS Sample Buffer (4X) + 2-Mercaptoethanol in 2:1) and loaded onto a precast Gel (NuPAGE™ 4-12% Bis-Tris Gel, Invitrogen). Proteins were transferred to nitrocellulose membranes ...
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bioRxiv - Microbiology 2023Quote: ... Midguts were incubated with primary antibody anti-flavivirus envelope 4G2 antibody produced in-house in 1X PBS at 4 °C overnight followed by 1:500 secondary antibody anti-mouse IgG Alexa 488 (Invitrogen, A28175) in 1X PBS and Hoechst33342 (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... and 4 µL (<1 µg) of DNase treated total RNA was then reverse transcribed into cDNA (Superscript III Supermix, Thermo Fisher) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... incubated in secondary antibody solution for 4 hours at RT (5% normal goat serum in PBST, Alexa Fluor 633 anti-guinea pig 1:200 (Thermo Scientific)) ...
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bioRxiv - Bioengineering 2023Quote: ... followed by washing and addition of secondary antibodies and 4′,6-diamidino-2-phenylindole (DAPI) (1:1000, Thermo Fisher Scientific, 62248) for 30 mins ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were then incubated for 30 min at 4 °C with AF647-conjugated goat anti-mouse IgG (1:500, Thermo Fisher). Cholesterol levels were determined by incubation for 30 min at 4 °C with filipin (50 µg/mL) ...
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bioRxiv - Bioengineering 2023Quote: ... to each well we add 200 μL of 1 μg/mL 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, Cat. No. D3571) solution in DI water ...
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bioRxiv - Developmental Biology 2023Quote: ... for one hour at RT and stained overnight at 4°C with the primary antibody rabbit anti-GFP diluted 1:500 (#A11122 Thermo Fisher). Samples were washed three times using 0.3% PBT and stained with secondary antibody Alexa Fluor 488 Goat anti-Rabbit diluted 1:1000 (#R37116 ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with the following antibodies: mouse anti-Beta-actin (1:1000, Thermo Fisher Scientific, AM4302) and rabbit anti-PROX1 (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... in 5% FBS overnight at 4°C and Alexa Fluor® 488 goat anti-mouse IgG secondary antibody (1:200; #10696113, ThermoFisher) for one hour at RT ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated O/N at 4°C with gentle agitation with 1:2000 goat anti-human complement C3 antibody (Thermo Scientific) diluted in 1X TBS/0,5% Tween/3% BSA ...
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bioRxiv - Systems Biology 2023Quote: ... the cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI, 1 μg/mL, Thermo Fisher Scientific, Waltham, MA, USA) in PBS for 15 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated for 2 hours at 4°C in a 1:1000 solution of AF-568 donkey anti-mouse secondary antibody (Invitrogen #A10037) in blocking solution ...
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bioRxiv - Developmental Biology 2023Quote: ... and then incubated ON at 4°C with the following primary antibodies in blocking solution: chicken anti-GFP (Invitrogen, 1:1000), rabbit anti-somatostatin (BMA-biomedials ...
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bioRxiv - Immunology 2023Quote: ... The samples were then stained with the blood lineage antibody panel (Table 1) for 30 minutes at 4°C and then washed with PBS (ThermoFisher Scientific) and 2% (v/v ...
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bioRxiv - Immunology 2023Quote: ... was incubated overnight at 4°C and Alexa Fluor 488 goat anti-chicken IgY (H+L) secondary antibody (1:100, A-11039, ThermoFisher Scientific) was applied to detect GFP+ cells ...