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Citations for Thermo Fisher :
5901 - 5950 of 10000+ citations for 7 METHOXY 4 4 DIMETHYL 3 4 DIHYDRO 2H NAPHTHALEN 1 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The sonicated cells were centrifuged at 14,000 rpm for 1 h at 4°C to remove the cell debris and the supernatant was incubated to Ni2+-NTA resin (Thermo Fisher) pre equilibrated with lysis buffer ...
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bioRxiv - Neuroscience 2020Quote: ... then placed overnight at 4 0C in rabbit anti-GFP primary antibody (1:1000 in blocking solution, Invitrogen, La Jolla, CA). Following incubation ...
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Observation of an α-synuclein liquid droplet state and its maturation into Lewy body-like assembliesbioRxiv - Molecular Biology 2020Quote: ... overnight at 4 °C and visualised with a fluorescent secondary antibody (goat-anti-mouse IgG Alexa 488-conjugated (1:5000, A11029, Life Technologies)) ...
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bioRxiv - Microbiology 2020Quote: ... Differentiated THP-1 cells are adherent and were seeded in 4-well Lab-TekII Chamber Slides (Nalgene Nunc International, Rochester, NY) at a density of 3 × 105 cells per well ...
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bioRxiv - Systems Biology 2020Quote: ... The following primary antibodies were diluted in the blocking solution and applied overnight at 4°C: mouse anti-TAU clone HT7 (1:500, Thermo Scientific), chicken anti-MAP2 (1:10,000 ...
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bioRxiv - Cell Biology 2021Quote: ... 10 ml of culture was transferred to a foil wrapped tube and incubated with FM 4-64 (1 µM in DMSO, Molecular Probes) for 30 min at 30°C ...
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bioRxiv - Bioengineering 2022Quote: DNA transfections were performed by seeding HEK293T cells in 12 well plates at 25% confluency and adding 1 μg of each DNA construct and 4 μL of Lipofectamine 2000 (Thermo Fisher). RNA transfections were performed by adding 1 μg of each RNA construct and 3.5 μL of Lipofectamine MessengerMax (ThermoFisher) ...
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bioRxiv - Cell Biology 2022Quote: ... Nuclei were lysed by adding 1:4 SDS buffer (2% SDS, EDTA) and samples were sonicated (5×1s pulses, 80% power, Fisher Scientific). Samples were diluted to 0.2% SDS with TX-100 lysis buffer and centrifuged for 20 min at 20,000 x g ...
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bioRxiv - Neuroscience 2022Quote: ... after which gels were transferred onto Amersham Protran 0.2 µm nitrocellulose membrane (Dutscher, 10600001) immersed at 4°C in NuPAGE Transfer Buffer (ThermoFisher, NP0006-1) for 90 minutes at 60V ...
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bioRxiv - Molecular Biology 2022Quote: ... the beads were separated from the lysate and washed four times with 1 ml of CoIP buffer before resuspending in 15 μl of 1 x loading buffer (NuPAGE LDS sample buffer (4 x) and NuPAGE sample reducing agent (10 x) (ThermoFisher Scientific) diluted to 1 x with H2O) ...
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bioRxiv - Genomics 2022Quote: ... resuspended in ice cold PBS+BE (1 mg/mL BSA + 2 mM EDTA) and incubated for an hour at +4°C with Dynabeads Biotin Binder (Thermo Fisher). Cells were repeatedly washed with ice-cold sterile PBS while on magnet ...
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bioRxiv - Neuroscience 2022Quote: ... Incubation of sections from cerebral organoids in primary antibodies solution was performed overnight at 4°C and in secondary antibodies solution at room temperature for 1 hour (Alexa Fluor, Molecular Probes). The following primary antibodies were used ...
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bioRxiv - Developmental Biology 2022Quote: ... centrifuged for 3 min at 300g at 4°C and resuspended in 1 ml pre-warmed enzymatic mix of 5 mg/ml Dispase (Gibco, 17105041) and 0.5 mg/ml Liberase TH Research Grade (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... which were subsequently vitrified at 4°C and 100% humidity for 4.5 s with a blotting force of -1 on a Vitrobot IV (Thermo Fisher Scientific) with standard Vitrobot filter paper (ø55/20 mm ...
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bioRxiv - Immunology 2022Quote: ... cells were treated with antibodies (100 or 10 nM) for 1 h at 4°C and then stained Alexa647-conjugated goat anti-human IgG (Invitrogen, A21445) for 0.5 h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... This was followed by blocking and incubation with appropriate primary antibodies overnight at 4 °C and species specific secondary antibodies [polyclonal Alexa fluor 488 goat anti-rabbit,1:1000 (A11070 of Invitrogen Biosciences), polyclonal Alexa fluor 555 rabbit anti-mouse,1:1000 (A21427 of Invitrogen Biosciences)] for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Free-floating slices were then incubated 48h at 4° with a mix of anti-GFP (1:5000, Ref. A10262, Invitrogen, USA) and anti-NeuN (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then incubated for two days at 4°C with rabbit monoclonal anti-GFP antibody (1:2,000; Invitrogen, Waltham, USA) or rabbit polyclonal anti-c-fos antibody (1:2,000 ...
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bioRxiv - Plant Biology 2021Quote: ... US) with an input ratio of 4:1 (pENTR4:pHellsgate8) were used to shuttle inserts into pHellsgate8 (Invitrogen, Carlsbad, CA, US). Prepared plasmids were used to transformed E ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then washed twice with ice cold PBS (4° C) and subsequently transferred to cold PBS containing Ez-link Sulfo-NHS-LC-biotin (1 mg/ml; ThermoFisher Scientific) for 45 min ...
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bioRxiv - Molecular Biology 2020Quote: ... overnight at 4° C followed by goat anti-rabbit Alexa fluor 594 secondary antibody (1:400, Invitrogen, Carlsbad, CA, cat. A1102) incubation for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... acute hippocampal slices were labeled for 30 min at 4 °C with 1 mg/ml sulfo-NHS-SS biotin (ThermoFisher Scientific). Membranes were prepared and the biotinylated proteins were precipitated with streptavidin agarose resin (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... 1 ml of mannan-DAP was added to 50 × 106 beads and then mixed with 4 mg/1 ml of EDC (ThermoFisher Scientific) resuspended in pure water ...
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bioRxiv - Neuroscience 2021Quote: ... overnight at 4 °C and with horseradish peroxidase-conjugated rabbit anti-goat secondary antibody (1:3000; Thermo Fisher Scientific, Waltham, MA) for 1 h at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... cells were resuspended in fresh RPMI medium and stained with CellTracker Green CMFDA (1:1000 v/v in -PBS per 4 x 106 cells, Thermo Fisher) for 10 min at 37°C in a water bath ...
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bioRxiv - Microbiology 2021Quote: ... 10 x 250 mm) at a flow rate of 4 ml min−1 using a Dionex UltiMate 3000 system (Thermo Scientific) fitted with a UV detector optimized to detect the O-phenyl functional group of the acceptor molecule at 270 nm ...
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bioRxiv - Neuroscience 2020Quote: ... primary antibodies were incubated for three nights at 4°C followed by incubation with donkey anti-mouse or anti-rabbit IgG secondary antibodies (1:1000, Life Technologies). Fluorescent images were acquired with an Olympus FV1000 at 2048 x 2048 pixel resolution using a 40x objective lens ...
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bioRxiv - Bioengineering 2020Quote: ... samples were fixed with 4% paraformaldehyde (w/v) in PBS for 1 h and stained with Actin Red 555 (Molecular Probes, ThermoFisher) for 1h ...
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bioRxiv - Cancer Biology 2020Quote: ... Captured cells were stained with 2 μM green-fluorescent calcein-AM and 4 μM red-fluorescent ethidium homodimer-1 (Molecular Probes) and visualized under an EVOS FL cell imaging station (Life Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed 3x with 1x PBS (5 minutes per wash) prior to 4’,6-diamidino-2-phenylindole (DAPI; 1:3000; Thermo Fisher) incubation for 5 minutes at room temperature to stain nuclei ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were rinsed three times in the blocking solution and incubated for 4 hours in goat polyclonal secondary antibody to mouse IgG conjugated with AlexaFluor 555 (1:100, Invitrogen A31570) and donkey polyclonal secondary antibody to rabbit IgG conjugated with AlexaFluor 647 (1:100 ...
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bioRxiv - Microbiology 2022Quote: ... Cell Signaling)] at 1:1,000 in blocking buffer overnight at 4°C with rocking and subsequently with HRP-conjugated goat α-rabbit or α-mouse secondary antibodies at 1:5,000 (ThermoFisher) in blocking buffer for 2 h at room temperature with rocking ...
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bioRxiv - Molecular Biology 2022Quote: ... spun at 4000 rpm for 15 minutes at 4 °C and the cell pellet was dissolved in 1 mL TRIzolR (Invitrogen, #15596026). An equal volume of ethanol was added to the mixture and total RNA was prepared using a Direct-zol™ ...
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bioRxiv - Immunology 2022Quote: ... Denatured tri-S protein (50 µg total) was separated by SDS-PAGE with a NuPAGE® 4-12% Bis-Tris Gel (1-well, Invitrogen), electro-transferred onto nitrocellulose membranes ...
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bioRxiv - Evolutionary Biology 2022Quote: ... All slides were then rinsed three times with PBS-T and twice with 1× PBS and coated with 4’,6-diamidino-2-phenylindole (DAPI) containing the antifade reagent ProlongGold (Life Technologies). Images were acquired using an Olympus BX63 automated fluorescence microscope equipped with an Olympus DP74 digital camera and evaluated with cellSens Dimension software (Olympus).
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bioRxiv - Developmental Biology 2022Quote: ... and dissociated to single cell via incubation in Tryple Express Enzyme (12-604-013, Thermo Fisher Scientific; 1 ml /4 limbs) for 15 minutes at 37 °C on a shaker ...
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bioRxiv - Genomics 2019Quote: Single cells were stained with 4 μM Ethidium homodimer-1 and 2 μM Calcium AM (ThermoFisher LIVE/DEAD Viability Kit L3224) in the dark at room temperature for 20 min ...
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bioRxiv - Genetics 2019Quote: ... qRT-PCR was performed with the cDNA template diluted 1:4 with nuclease-free water then pipetted into quadruplicate wells of a 384 well optical plate (Cat#4309849 Applied Biosystems). Thermo Maxima SYBR Green/Rox qPCR Master Mix 2X (Cat#K0221 ...
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bioRxiv - Developmental Biology 2019Quote: ... and sections were incubated overnight at 4°C with the following primary antibodies: rabbit anti-GFP (Invitrogen A-11122, 1:1000), mouse anti-Satb2 (Abcam ab51502 ...
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bioRxiv - Microbiology 2019Quote: ... waxworms were anesthetized at 4°C for ten minutes then ground in 1 mL of TRIzol™ (Invitrogen™, Carlsbad, CA) using the sterile 15 mL conical tissue grinder (VWR™ ...
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bioRxiv - Neuroscience 2019Quote: ... tissues were incubated overnight at 4°C in Alexa Fluor 488-conjugated goat anti-rabbit Ab (1:200) secondary antibody (Life Technologies) in PBS containing 10% normal sheep serum ...
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bioRxiv - Developmental Biology 2020Quote: ... overnight at 4°C and further stained with 1:200 Alexa Fluor 633 Goat anti-Rabbit IgG (H+L) Secondary Antibody (Invitrogen A21071) at a 20°C for 3 hours ...
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bioRxiv - Neuroscience 2019Quote: ... for 80 minutes (4 × 20 min) at room temperature before secondary antibodies (donkey anti-rabbit Alexa Fluor 1:1000, Life Technologies Cat No ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated overnight at 4°C with an anti-CD63 primary antibody (1:20 dilution, Thermo Fisher Scientific, cat. # MA1-19281) in blocking buffer ...
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bioRxiv - Systems Biology 2020Quote: ... cells were stained at 4°C o/n in 50 µL of the 1st antibody diluent (rabbit anti-ZO-1, 61-7300, Thermo Fisher Scientific Inc. ...
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bioRxiv - Synthetic Biology 2020Quote: ... cells were passaged 1:4 into a new 15 cm dish and grown in DMEM supplemented with 10% FBS (Thermo Fisher) and Hygromycin (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... thoroughly rinsed twice with PBS and coated overnight at 4◦C with 100 µl/well of 1 mg/ml biotinylated BSA (Thermo Fisher) in PBS ...
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bioRxiv - Immunology 2020Quote: ... thoroughly rinsed twice with PBS and coated overnight at 4°C with 100 μl/well of 1 mg/ml biotinylated BSA (Thermo Fisher) in PBS ...
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bioRxiv - Microbiology 2020Quote: ... then 1 mL of a - 20°C pre-chilled precipitation solution was added (50% v/v acetone [A949-4; Fisher Scientific, Fair Lawn ...
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bioRxiv - Microbiology 2021Quote: ... Initially all low-quality regions and gaps were targeted with computationally selected Sanger sequencing reactions completed with 4:1 BigDye terminator: dGTP chemistry (Applied Biosystems). These automated rounds included walking on 3 kb and 8 kb plasmid subclones and fosmid clones using custom primers (400 ...