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Citations for Thermo Fisher :
5951 - 6000 of 10000+ citations for 7 METHOXY 4 4 DIMETHYL 3 4 DIHYDRO 2H NAPHTHALEN 1 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... Chromatin with antibody were rotated overnight at 4 °C before 1:40 protein A/G agarose beads (Thermo Fisher Scientific, #20423) were added and incubated for another 6 hrs ...
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bioRxiv - Cell Biology 2020Quote: ... 1:200) respectively at 4°C overnight and secondary antibody conjugated to Alexa Fluor 594 or Alexa Fluor 488 (Life Technologies) at room temperature for 1h ...
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bioRxiv - Cell Biology 2020Quote: ... and either pINDUCER-20-IRS2 or pHAGE-FLAG-HA-NTAP-IRS2 in a 4:2:1 DNA ratio using Lipofectamine 3000 (Invitrogen, L3000001) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... Sections were incubated overnight (4 °C) with the respective primary antibodies: Anti-Ly6G rat monoclonal (1:100; Affymetrix, Santa Clara, CA) for neutrophils ...
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bioRxiv - Plant Biology 2019Quote: ... The slides were incubated overnight at 4°C with a primary antibody specific to GFP (1/200, Life Technologies, A-11122) then for 2 hours with goat-anti-rabbit-AlexaFluor488 secondary antibody (Life Technologies ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and subsequently incubated with 1 mg/mL lysozyme at 4 °C for 30 min and further broken with French press (Thermo Scientific). The total lysate was centrifuged at 98,000 × g for 30 min at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... at 37°C for 20 min and stained with DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate, 1:1000 in PBS, pH 7.4, Thermo Fisher Scientific) before imaging on a Nikon Eclipse Ti-E inverted microscope with a 100X lens (Apochromat 1.49 Oil 0.13-0.20 DIC N2 ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated for 4 h at room temperature in fluorescent conjugated secondary antibodies (Alexa Fluor 488 goat anti-mouse (Th; 1:500; Invitrogen; A10667) and Alexa Fluor 594 goat anti-rabbit (DsRed ...
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bioRxiv - Immunology 2021Quote: ... Peptide and enzyme solutions were dissolved and diluted in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific). The peptide (100 μM in a total volume of 100 μL ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed with PBS and lysed at 4°C in ice-cold 1% triton lysis buffer supplemented with Pierce protease inhibitor tablet (Life Technologies) and phosphatase inhibitors ...
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bioRxiv - Cell Biology 2019Quote: ... then further cleared by centrifugation at 4°C and 25658 g for 1 h (Thermo Fisher Scientific, #A23-6×100 rotor). The supernatant was passed through a 0.22 µm filter (Corning #431097 ...
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bioRxiv - Neuroscience 2021Quote: ... prepared in 5% NGS in DPBS overnight at +4 following an incubation with Alexa Fluor405 (A31556, Thermo Fisher Scientific, 1:500) or Alexa Fluor680 (A31556 ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 2 days at 4°C with rabbit anti-GFP antibody (1:2,000; Thermo Fisher Scientific, USA). The sections were then incubated with biotinylated donkey anti-rabbit IgG antibody (1:1,000 ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were washed with 1x PBS and secondary antibodies diluted in blocking solution were added 4 hrs at RT: anti-Rabbit 555 (1:800; Invitrogen; A31572), anti-Chicken Cy2 (1:800 ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were incubated overnight in a humidified chamber at 4 °C in TBS-T with 1% Fatty acid free-BSA (Fisher Scientific) with anti-mouse/human antibodies ...
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bioRxiv - Biochemistry 2021Quote: ... resuspended in 100 μL of secondary antibody (1:500 α-mouse IgG-Alexa647 [ThermoFisher, Waltham, MA] in TBST with 4% BSA), and incubated in the dark for 60 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... the following primary antibodies were diluted in 10 mL of 1.5% BSA in TBST containing 0.0005% sodium azide and incubated at 4 °C with the membranes overnight: α-GFP (1:2000, rabbit pAb, Life Technologies); α-Abl (1:1000 ...
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bioRxiv - Biochemistry 2020Quote: ... or mouse monoclonal anti-N-Cadherin clone GC-4 followed by AlexaFluor 647-conjugated goat anti-mouse antibody (1:200 dilution, ThermoFisher Scientific). Staining was performed for 30 min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... embryos were washed five times in TBSN/BSA for one hr at 4°C whilst rotating and incubated with secondary antibodies overnight at 4°C at a dilution of 1:400 (Alexa Fluor 488 goat anti-mouse (Invitrogen, #A11001) and 1:400 Poly-HRP-conjugated secondary antibody from Tyramide SuperBoost Kits with Alexa Fluor 568 (ThermoScientific ...
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bioRxiv - Immunology 2021Quote: ... targeting four different T cell populations as well as 4 μg mL−1 of goat-anti-mouse IgG AF488 secondary antibody (Thermo Scientific). Acquisition and data analysis were performed as mentioned above.
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bioRxiv - Immunology 2020Quote: ... 25mM HEPES (2(−4-(2-hydroxyethyl)-1-piperanzinyl) ethansulfonacid) and supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco, ThermoFisher Scientific), 10.000 U/mL (v/v ...
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bioRxiv - Immunology 2020Quote: Splenic B cells were resuspended at a concentration of 2.5-5 × 106 cell/mL in RPMI supplemented with 20 mM HEPES and 2.5% FCS and loaded with 1 μM Fluo-4 (F14201, Thermo Fisher Scientific) and 3 μM Fura Red (F3021 ...
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bioRxiv - Immunology 2021Quote: ... limbs were incubated in 5% sucrose (1 hour) and 30% sucrose (overnight) at 4°C and then embedded in OCT medium (Cat. # 23-730, Fisher Scientific). Transverse cryosections (12 μm ...
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bioRxiv - Immunology 2021Quote: ... overnight at 4°C followed by incubation for 1 hour with secondary goat anti–mouse IRDye 800 (Thermo Fisher Scientific, MA) or goat anti–rabbit Alexa Fluor 680 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... and effector cells were co-cultured for 4 hr at 37°C then fixed in 1% paraformaldehyde (Affymetrix, Santa Clara, CA). Cells were analyzed by flow cytometry (Symphony I/II ...
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bioRxiv - Neuroscience 2020Quote: ... 0.3% Triton X-100 in PBS) and incubated overnight (4 °C) with primary antibodies against TH (OPA1-04050 1:1000, Thermo Scientific) and HA (3F10 1:200 ...
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bioRxiv - Bioengineering 2021Quote: ... injected into a petri dish and incubated with 2 µM calcein AM and 4 µM EthD-1 (Live/Dead Viability/Cytotoxicity Kit, Life Technologies) in PBS++ for 30 minutes at 37 ° C ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed for 1 h at 4°C using a FOXP3/Transcription Factor Staining Buffer Set (Invitrogen, for FOXP3 detection) or 15 min at room temperature using a 1.85% formaldehyde solution in PBS (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... Streptavidin pull-down was carried out overnight at 4°C in 1 ml volume containing 60 μl of streptavidin magnetic beads (Life Technologies), 1 % NP40 ...
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bioRxiv - Cell Biology 2022Quote: ... Cell suspensions were concentrated to 1 × 106 cells/mL in PBS and centrifuged onto glass slides using a Cytospin 4 cytocentrifuge (Thermo Scientific). Fixed cells were permeabilized with 0.3% Triton X-100 in PBS for 5 min and incubated with Triton Block (0.2 M glycine ...
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bioRxiv - Microbiology 2022Quote: ... Lungs were inflated with 1 ml of a solution containing 1 part 4% PFA/30% sucrose and 2 parts of optimal cutting temperature compound (OCT) media (Fisher Scientific). Inflated lungs were tied off and stored in 4% PFA/30% sucrose at 4°C overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... The gonads were washed 3 times and then stained with 1 μg/mL 4’,6-diamidino-2-phenylindole (DAPI) before mounting with antifade solution (Invitrogen, P36980) on poly-L-lysine coated glass slides (Sigma Merck ...
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bioRxiv - Bioengineering 2022Quote: ... Hydrogels were washed 4×20 minutes with PBST and then incubated for 30 minutes in Hoechst (1:2000; Thermo Fisher H3570). Samples were washed a final time in PBST and were stored in PBST until imaged.
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bioRxiv - Cancer Biology 2022Quote: ... The stock solutions of the agonists were made in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific) and the enzyme inhibitors were reconstituted according to manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... We collected viral supernatants after 48 h and used them to infect the Jurkat human cell line by spinoculation with 4 μg ml-1 Polybrene Infection/Transfection Reagent (Fisher Scientific). We selected infected human cell lines with 1 mg ml-1 blasticidin (InvivoGen ...
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bioRxiv - Genomics 2022Quote: ... Sorted cells were centrifuged at 300g for 10 minutes at 4°C and resuspended in 1 ml of TRIzol (Invitrogen, 15596018). Spermatogonial RNA was purified according to the standard TRIzol protocol and contaminant genomic DNA was digested using the DNA-free kit (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... Primary antibodies were incubated overnight at 4°C and included mouse anti-3OH-K (1:1000; MA1-16616, AB_568420, clone P3UI, Thermo Fisher Scientific) and mouse anti-β-Tubulin E7 (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... Total lipids were isolated by Folch extraction116 by adding 24 mL 2:1:0.6 HPLC chloroform (C297-4, Thermo Fisher Scientific): HPLC methanol (A454-4 ...
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bioRxiv - Neuroscience 2022Quote: ... blocked again for 2h (10% CAS-Block / 90% PBT) and incubated overnight at 4°C with secondary antibody (1:250, Alexa-Fluor-594, ThermoFisher A32742) diluted in 100% CAS-Block ...
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bioRxiv - Genomics 2022Quote: ... and the resulting pellet was suspended in 0.85% NaCl (1.5 mL) and centrifuged (10,000 g, 1 min, 4°C) using Heraeus Primo R Centrifuge (Thermo Scientific, Schwerte, Germany). Pellets from the two tubes were dissolved in high salt buffer (1 mL ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with rabbit anti-goat IgG conjugated with Q-dot 655 for 2 h at 4 °C (1:350 dilution; Life Technologies). Then ...
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bioRxiv - Neuroscience 2022Quote: ... Cortical neurons were pelleted at 2,500 x g for 5 minutes at 4 °C and resuspended in 1 mL Neurobasal outgrowth media (Thermo Scientific, 21103049), supplemented with B-27 (Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: ... brasiliensis strains were maintained weekly by subcultures on brain-heart infusion agar (BHI - Duchefa, Netherlands) supplemented with 1% glucose and 4% FBS (Life Technologies) or YPD agar (Yeast extract - Peptone - Dextrose ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were then incubated for 4 days at 37°C with a secondary antibody (donkey anti-rabbit AF488, Invitrogen, 1:1,000) in 1X PBS with 0.2% Tween 20 ...
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bioRxiv - Bioengineering 2022Quote: ... 150 mM NaCl and were allowed to incubate with the dye for 1 hour at 4 ºC before being loaded into a 384-well qPCR plate (ThermoFisher, BC2384). The plate was then placed into a CFX384 Touch Real-Time PCR Detection System (BioRad ...
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bioRxiv - Bioengineering 2022Quote: Staining of the cells was performed by incubating the cells in PBS with 4 μm Calcein UltraBlue AM (Cayman Chemi-cal) or with 1 μm Calcein AM (Invitrogen™) for 1 h ...
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bioRxiv - Biophysics 2024Quote: ... VivoTrax-labeled NK-92 cells were centrifuged onto Cytospin glass slides at a density of 1 x 105 cells (Cytospin 4 centrifuge, Thermo Scientific). Attached cells were then fixed by methanol/acetic acid solution (3:1 ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated over-night at +4°C with donkey anti-sheep secondary antibody conjugated with Alexa Fluor 568 (1:1000, A-11011, Invitrogen, ThermoFisher Scientific) and Streptavidin conjugated with Alexa Fluor 488 (1:2000 ...
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bioRxiv - Pathology 2024Quote: ... in DMEM (high-glucose, containing pyruvate, 10313021, FisherScientific) and subcultured in 1:4 ratio using Gibco TrypLETM Express Enzyme (12605010, Fisher Scientific) for cell dissociation ...
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bioRxiv - Neuroscience 2024Quote: ... The following day sections were rinsed three times in PBTriton 0.5% and incubated overnight at 4°C in secondary antibody solution using Goat anti-Rabbit-Oregon Green 488 (1:500 Invitrogen, 011038). Next ...