Labshake search
Citations for Thermo Fisher :
6001 - 6050 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... Amplified cDNAs were diluted 1:15 in ultra-pure water and subjected to real-time polymerase chain reaction (PCR) on an Applied Biosystems Model 7900HT with TaqMan Universal PCR Mastermix (Applied Biosystems, Foster City, CA) and the following Taqman probes ...
-
bioRxiv - Pathology 2023Quote: ... The cDNA was used for RT-qPCR with qPCRBIO SyGreen Blue mix Hi-ROX (PCR Biosystems, London, UK) on the StepOne Plus Real-Time PCR machine (Applied Biosystems, Foster City, CA) and primers listed in Table S2 ...
-
bioRxiv - Cell Biology 2024Quote: ... was performed for each sample in triplicate using the Step One Plus PCR System or the QuantStudio 12K Flex Real-Time PCR System using the TaqMan Gene Expression Assay (Applied Biosystems, Thermo Fisher Scientific, USA), with a reaction volume of 5uL ...
-
bioRxiv - Neuroscience 2024Quote: ... qPCR was performed on a 7500 Real-time PCR System (Applied Biosysems, Foster City, CA) utilizing SYBR Green PCR Master Mix reagents (Applied Biosystems, Foster City, CA). Rat GAPDH (F:AGG TTG TCT CCT GTG ACT TC ...
-
bioRxiv - Cell Biology 2021Quote: ... Real-time qPCR was performed using SYBR Green (BIO-RAD and a ViiA 7 Real Time System (ThermoFisher, Waltham, MA, USA) as specified by the manufacturer ...
-
bioRxiv - Neuroscience 2024Quote: ... The real-time quantitative polymerase chain reaction (Real-Time qPCR) was performed using SYBR Green and the 7300 RealTimePCR system (Applied Biosystems). All data were analyzed using the delta-delta Ct approach ...
-
bioRxiv - Microbiology 2021Quote: ... RT-qPCR was then performed using the AgPath RT-PCR (Life Technologies) kit on a QuantStudio™ 7 Flex Real-Time PCR System with the primers specific for SARS-CoV-2 E gene 48 ...
-
bioRxiv - Microbiology 2022Quote: ... RT-qPCR was then performed using the AgPath RT-PCR (Life Technologies) kit on a QuantStudio™ 7 Flex Real-Time PCR System with the primers specific for SARS-CoV-2 E gene58 ...
-
bioRxiv - Cell Biology 2022Quote: ... 7µL of RT-PCR mix (Superscript III RT/platinium Taq 0,1µL (Invitrogen), Reverse and Forward primers and spikes at 1,33µM final concentration and homemade 2X reaction Mix (120mM Tris SO4 pH=9 ...
-
bioRxiv - Genomics 2020Quote: ... Standard quantitative RT-PCR (TaqPath 1-step RT qPCR Master Mix, Thermofisher) was run with test RNA and gene-specific standard curves (2e5 copy/mL – 20 copy/mL) ...
-
bioRxiv - Molecular Biology 2020Quote: ... The cDNA products were analyzed on a StepOnePlus Real-time PCR system using SYBR Select Master Mix (Applied Biosystems). The primers used for different target genes are ...
-
bioRxiv - Cancer Biology 2021Quote: The on-target indel frequencies were quantified using QuantStudio™ 12K Flex Real-time PCR System (Thermo Fisher Scientific) with Power SYBR Green Master Mix (Thermo Fisher) ...
-
bioRxiv - Microbiology 2020Quote: ... Quantitative polymerase chain reaction was performed with a QuantStudio 3 Real-Time PCR instrument (Thermo Fisher Scientific, Waltham, MA) using SYBR Green detection chemistry ...
-
bioRxiv - Molecular Biology 2019Quote: ... functional primers with the corresponding probes were tested by qPCR using a StepOne Real-Time PCR instrument (Applied Biosystems). The following reagents were added for a 20 μL reaction mixture ...
-
bioRxiv - Molecular Biology 2020Quote: ... Nick primers were used with SYBR® Green on the Step One plus Real-time PCR system (Applied Biosystems) and normalized to the Ct values obtained within the surrounding ∼300 bp DNA region amplified with the flanking primers (Table S2) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Real-time PCR (qPCR) was caried out using IQ-SYBR Green in a Step One Thermal Cycler (Applied Biosystems). Specific primers used are indicated in Supplementary Table 2 ...
-
bioRxiv - Cell Biology 2020Quote: ... on a Step One Plus Real-Time PCR thermal cycler (Step One software v2.2.2; Applied Biosystems, Thermo Fisher Scientific). The Taqman gene expression assays for the reference gene (GAPDH – ID# Hs02786624_g1 ...
-
bioRxiv - Cell Biology 2020Quote: ... on a Step One Plus Real-Time PCR thermal cycler (Step One software v2.2.2; Applied Biosystems, Thermo Fisher Scientific). The Taqman gene expression assays for the reference gene (GAPDH – ID# Hs02786624_g1 ...
-
bioRxiv - Cell Biology 2020Quote: Transfection efficiency and altered gene expression were analysed using SBYR Green Real-Time PCR Master Mix (A25741; Applied biosystems). Briefly total RNA was extracted from cells using TRIzol (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: ... Equal amounts of each cDNA were analyzed by real-time PCR with specific primers and PowerUp SYBR Green (Invitrogen) in an ABI QuantiStudio 3 real-time PCR system ...
-
bioRxiv - Immunology 2021Quote: ... 2020) and reactions were carried out using a QuantStudio 6 and 7 Flex Real-Time PCR system (Applied Biosystems) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... according to the manufacturer’s instruction and quantitative real-time PCR was performed using Taqman Gene expression assays (Applied Biosystems) to analyze expression of SARM1 (Rn01750947_m1) ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Libraries were quantified using a ViiA™ 7 Real-Time PCR System (Applied Biosystems Corp., Foster City, CA, USA). Libraries were then pooled and diluted to 4 nM and submitted for sequencing.
-
bioRxiv - Evolutionary Biology 2020Quote: ... Real-Time PCR was performed with ABsolute Blue QPCR Mix low ROX 1X (Thermo Fisher Scientific, Waltham, MA, USA) and 2.5 µL of cDNA under the following thermal conditions ...
-
bioRxiv - Microbiology 2022Quote: ... All qPCR reactions were run and analyzed on the QuantStudio 3 Real-Time PCR System with analysis software v1.5.1 (Applied Biosystems). Each timepoint was collected in biological triplicates with qPCR reactions from each experimental sample done in technical triplicates.
-
bioRxiv - Neuroscience 2021Quote: ... following titration with real-time PCR (rAAV titers about 1.0–6.0 × 1012 viral genomes/mL, TaqMan Assay, Applied Biosystems). To visualize amyloid plaques ...
-
bioRxiv - Molecular Biology 2021Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was performed by using an ABI Prism® 7500 (Applied Biosystems) or Thermal Cycle Dice Real Time System (TP850 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Datasets were collected on an Applied Biosystems™ QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher Scientific). The cycling conditions were as follows ...
-
bioRxiv - Molecular Biology 2020Quote: ... Quantification of gene expression was performed using a 7900 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). Threshold values (CT ...
-
bioRxiv - Molecular Biology 2020Quote: The enrichment of specific DNA fragments was analysed by real-time PCR using a QuantStudio 3 Cycler (Applied Biosystems). The results of chromatin immunoprecipitation are presented as a percentage of input genomic DNA after triplicate PCR measurements ...
-
bioRxiv - Immunology 2021Quote: ... Targeted qPCR was performed using the QuantStudio 6 Real-Time PCR system using the following Taqman Primers (Applied Biosystems): BCL6 (Hs00153368_m1) ...
-
bioRxiv - Cell Biology 2019Quote: ... Real-time PCR for Saa3 was performed using the TaqMan® assay (Applied Biosystems®, Grand Island, NJ, USA), catalogue number Mm00441203_m1 – Saa3 and β-actin (Actb) ...
-
bioRxiv - Genomics 2019Quote: ... Real-time PCR reactions were performed on a 1/40 cDNA dilution using Taqman Assays-on-Demand (Applied Biosystems) and Kapa Probe Fast Mastermix (Kapa Biosystems ...
-
bioRxiv - Cell Biology 2019Quote: ... Reactions were run on QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems, part of Life Technologies Corporation, CA) according to the manufacturer’s recommendations using a melt protocol in 0.05-degree/sec increments from 25°C to 95 °C ...
-
bioRxiv - Cancer Biology 2020Quote: ... according to manufacturer’s instructions in a 7900HT Fast-Real Time PCR System Instrument (Applied Biosystems, Grand Island, NY, USA). Primer pairs for the individual genes were obtained from the bioinformatically validated QuantiTect Library and are as follows ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... the level of P16 methylation was determined using an Applied Biosystems 7500 Real-Time PCR System (Applied Biosystems, USA) and normalized to that of the internal control gene ...
-
bioRxiv - Neuroscience 2021Quote: ... Analysis of expression profiles was done using StepOnePlusTM real-time PCR system (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) using the 2−ΔΔCT method56.
-
bioRxiv - Neuroscience 2021Quote: ... Analysis of expression profiles was done using StepOnePlusTM real-time PCR system (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) using the 2−ΔΔCT method56.
-
bioRxiv - Bioengineering 2021Quote: ... rAAV serotypes 1 and 2 viral particles were generated as described previously [40] and purified by AVB Sepharose affinity chromatography [41] after titration with real-time PCR (each titer 1.0 – 6.0E12 viral genomes/ml; TaqMan Assay; Applied Biosystems). For stereotaxic injection of rAAVs and the cranial window implantation ...
-
bioRxiv - Bioengineering 2020Quote: ... The melting temperature of the different fusion proteins was determined using a StepOne real-Time PCR system (Applied Biosystems) in combination with the Protein Thermal Shift™ kit (Applied Biosystems).
-
bioRxiv - Cancer Biology 2021Quote: Quantitative real-time PCR for ChIP assays (ChIP-qPCR) was performed using the SYBR Green Master Mix (Thermo Scientific) along with target-specific primers in optical 96-well plates on ABI StepOne Plus Thermocycler (Applied Biosystems ...
-
bioRxiv - Neuroscience 2020Quote: ... Quantitative real-time PCR assays were performed in Maxima SYBR Green/ROX qPCR master mix (Thermo Fisher Scientific, K0222) using a Thermo Fisher Scientific QuantStudio 3 Real Time PCR system under the following sequence of conditions ...
-
bioRxiv - Bioengineering 2021Quote: ... The cDNA was analyzed on an Applied Biosystems 7900HT Fast Real-Time PCR System (Applied Biosystems, Foster City, CA) in a 384-well microplate with the TaqMan™ Universal PCR MasterMix (Thermo Fisher Scientific ...
-
bioRxiv - Plant Biology 2020Quote: ... The qPCR was performed by QuantStudio™ 6 Flex Real-Time PCR machine (Applied Biosystems, Foster City, CA, USA). Elongation Factor 1α (CqEF1α)(XM_021888526.1 ...
-
bioRxiv - Microbiology 2020Quote: ... Forty cycles of amplification at 60 °C were conducted in a QuantStudio 3 Real-Time PCR System (Applied Biosystems). Amplification curves were analyzed using QuantStudio Design & Analysis Software v1.5.1 ...
-
bioRxiv - Plant Biology 2021Quote: ... PR1 expression was detected using primers qPR1_F and qPR1_R by quantitative real-time PCR using Maxima SYBR green mix (Thermo Fisher) and the AriaMx Real-Time PCR system (Agilent Technologies ...
-
bioRxiv - Microbiology 2021Quote: ... and quantified by real-time PCR with SYBR Green (DotScientific) using the one-step protocol QuantStudio 3 (ThermoFisher Scientific). Relative expression was calculated using the ΔΔCT method ...
-
bioRxiv - Microbiology 2021Quote: ... and quantified by real-time PCR with SYBR Green (DotScientific) using the one-step protocol QuantStudio 3 (ThermoFisher Scientific). Relative genomes were calculated using the ΔCT method ...
-
bioRxiv - Microbiology 2021Quote: ... Absolute numbers of copies of proviral DNA were determined by real time PCR using custom made probes (Thermo Fisher). To obtain absolute proviral DNA copy numbers ...
-
bioRxiv - Microbiology 2021Quote: ... Virus was quantified using real-time PCR using SYBR Premix Ex Taq™ (Applied Biosystems, Thermo Fisher Scientific, USA) according to the manufacturer’s instructions ...