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Citations for Thermo Fisher :
5951 - 6000 of 10000+ citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... with the StepOne Real-Time System (Applied Biosystems). TaqMan gene expression assays were used ...
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bioRxiv - Molecular Biology 2022Quote: ... on ABI7500 Real-time system (Applied Biosystems, USA). Rice U6 snRNA gene was used as the control for miRNA expression analysis ...
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bioRxiv - Neuroscience 2021Quote: ... on ABI 7900HT real-time system (Applied Biosystems). GAPDH was used as a reference gene for normalization and the relative expression differences were calculated based on the 2ΛΛCt method ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... using Real-Time QuantStudio7 Flex (Thermo Fisher Scientific). After 2 minutes at 50°C and 10 minutes at 94.5°C ...
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bioRxiv - Microbiology 2020Quote: Real time qPCR (SYBR®Green Applied Biosystems™ ...
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bioRxiv - Developmental Biology 2024Quote: ... RT-qPCR analysis was performed using Power SYBR Green RNA to CT 1 step RT-PCR kit (Applied Biosystems, #4389986) on a QuantStudio 3 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2019Quote: ... Quantitative real-time PCR reactions were performed with SYBR green PCR reagents using the ABI Prism 7300 sequence detection system (Applied Biosystems-Life Technologies, Waltham, MA). Relative gene expression levels were calculated using the 2 delta Ct method (Livak & Schmittgen ...
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bioRxiv - Neuroscience 2020Quote: ... qRT-PCR was performed using a TaqMan One-Step RT-PCR Master Mix Reagents Kit (ThermoFisher, cat. # 4309169) and primers for a genomic locus (b-actin ...
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bioRxiv - Pathology 2021Quote: ... The PCR mastermix was prepared using the AgPath-ID One-step RT-PCR kit (Thermo Fisher Scientific, USA) in a volume of 10 µl including 0.5 µl of β-Actin-mix2-HEX as internal control and 2.5 µl of extracted RNA ...
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bioRxiv - Immunology 2022Quote: ... Quantitative PCR was performed with the TaqPath™ COVID□19 CE□IVD RT□PCR Kit (Thermo Fisher Scientific) to obtain mean cycle threshold (Ct ...
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bioRxiv - Biochemistry 2023Quote: ... RT quantitative PCR was performed using the Applied Biosystems StepOnePlus™ RT PCR system (Thermo Fisher Scientific) with FastStart Universal SYBR Green Master (Roche Applied Science ...
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bioRxiv - Microbiology 2020Quote: ... miR-504-3p or miR-574-5p were reverse transcribed by using TaqMan™ Advanced miRNA cDNA Synthesis Kit (Thermo Scientific) and then amplified by using specific primers provided in the TaqMan Advanced miRNA Assays (Thermo Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... qRT-PCR was performed with 2x PowerUP™ SYBR™Green Master Mix in the QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems). GAPDH (AT1G13440 ...
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bioRxiv - Immunology 2020Quote: Quantitative PCR (qPCR) was performed using ViiA 7 Real-Time PCR System and Fast SYBR Green Master Mix reagent (Applied Biosystems, Thermo Fisher Scientific). Primer sequences are listed in Supplemental Table 1).
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bioRxiv - Neuroscience 2020Quote: ... using the Quant Studio OpenArray AccuFill platform and the PCR reactions were performed in a QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems, Foster City, CA).
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative real time PCR was performed using a SYBR green PCR master Mix according to the manufacturer’s instructions (Applied Biosystems, Foster city, CA, USA), on an ABI prism 7900 sequence detection system (Applied Biosystems) ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative real-time PCR was performed in triplicates using the QuantStudio 6 Flex Real-Time PCR System and Taqman Universal PCR Master Mix (Applied Biosystems, Foster City, CA). Probe sets for Il6 (Rn01410330_m1) ...
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bioRxiv - Microbiology 2022Quote: ... rRNA by qRT-PCR using TransScript Green One-Step qRT-PCR SuperMix (TransGen Biotech, Beijing, China) in a StepOnePlus Real-Time PCR System (Applied Biosystems, Waltham, MA, USA). Each reaction included 3 μL diluted cell lysate ...
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bioRxiv - Immunology 2022Quote: ... Equal volumes of cDNA were used for quantifying various cytokine immune response-related factor cDNAs via qRT-PCR using the StepOne real-time PCR system (Applied Biosystems, Foster City, California). qRT-PCR analysis was performed using KOD SYBR qPCR Mix (Toyobo Co ...
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bioRxiv - Neuroscience 2020Quote: ... A 10 µL reaction scale TaqMan method qPCR for Gapdh was performed using 1 µL of 1:40 diluted 20 cycle PCR products with 7900HT Fast Real-Time PCR System (Applied Biosystems, Foster City, CA) or CFX384 Touch Real-Time PCR Detection System (Biorad ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Quantitative real-time PCR (qRT-PCR) was performed in 384-well reaction plates using 2X TaqMan Fast Universal Master Mix (Applied Biosystems, Foster City, CA), 20X TaqMan specific gene expression probes (Applied Biosystems ...
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bioRxiv - Immunology 2019Quote: ... Quantitative PCR was performed using the PerfeCTa SYBR Green Fastmix (Quantabio, Beverly, MA) on a StepOne Real-time PCR system (Applied biosystems, Foster City, CA). PCR products were amplified with the following primer pairs ...
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bioRxiv - Genomics 2019Quote: The expression of mRNA transcripts was determined using cDNA reverse-transcribed from total RNA and quantitative PCR (qPCR) was performed on the QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA) using SYBR Green master mixes (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... Expression levels of SHANK3 were determined by TaqMan® qRT-PCR reaction using QuantStudio™ 12K Flex Real-Time PCR System (Thermo Fisher Scientific). The level of glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Molecular Biology 2022Quote: ... experiments were performed using an AB Applied Biosystems Step One Pulse Real-Time PCR System and TaqMan Universal PCR Master Mix (Applied Biosystems, Foster City, CA). The housekeeping gene GAPDH was used for all delta-delta Ct calculations as described earlier [57].
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bioRxiv - Developmental Biology 2024Quote: ... RT-qPCR was performed in 96-well plates (PCR-96-LP-AB-C, Axygen Inc.) with SYBR® green Real-Time PCR Master mix (Thermo Fisher Scientific Inc.). The PCR program included an enzyme activation step at 95 °C (10 min ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... qRT-PCR was performed using the PowerUp SyBr Green Master Mix on the QuantStudio™ 3 Real-Time PCR System (Applied Biosystems, Thermo Scientific). Amplification was performed using the rapid cycling mode following the manufacturer’s specifications.The primers were designed based on nucleic acid sequences obtained from the National Center for Biotechnology Information and the primer sequences are displayed in Supplementary Table S1 ...
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bioRxiv - Neuroscience 2023Quote: ... Amplified cDNAs were diluted 1:15 in ultra-pure water and subjected to real-time polymerase chain reaction (PCR) on an Applied Biosystems Model 7900HT with TaqMan Universal PCR Mastermix (Applied Biosystems, Foster City, CA) and the following Taqman probes ...
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bioRxiv - Pathology 2023Quote: ... The cDNA was used for RT-qPCR with qPCRBIO SyGreen Blue mix Hi-ROX (PCR Biosystems, London, UK) on the StepOne Plus Real-Time PCR machine (Applied Biosystems, Foster City, CA) and primers listed in Table S2 ...
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bioRxiv - Cell Biology 2024Quote: ... was performed for each sample in triplicate using the Step One Plus PCR System or the QuantStudio 12K Flex Real-Time PCR System using the TaqMan Gene Expression Assay (Applied Biosystems, Thermo Fisher Scientific, USA), with a reaction volume of 5uL ...
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bioRxiv - Neuroscience 2024Quote: ... qPCR was performed on a 7500 Real-time PCR System (Applied Biosysems, Foster City, CA) utilizing SYBR Green PCR Master Mix reagents (Applied Biosystems, Foster City, CA). Rat GAPDH (F:AGG TTG TCT CCT GTG ACT TC ...
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bioRxiv - Cell Biology 2021Quote: ... Real-time qPCR was performed using SYBR Green (BIO-RAD and a ViiA 7 Real Time System (ThermoFisher, Waltham, MA, USA) as specified by the manufacturer ...
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bioRxiv - Neuroscience 2024Quote: ... The real-time quantitative polymerase chain reaction (Real-Time qPCR) was performed using SYBR Green and the 7300 RealTimePCR system (Applied Biosystems). All data were analyzed using the delta-delta Ct approach ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was then performed using the AgPath RT-PCR (Life Technologies) kit on a QuantStudio™ 7 Flex Real-Time PCR System with the primers specific for SARS-CoV-2 E gene 48 ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was then performed using the AgPath RT-PCR (Life Technologies) kit on a QuantStudio™ 7 Flex Real-Time PCR System with the primers specific for SARS-CoV-2 E gene58 ...
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bioRxiv - Cell Biology 2022Quote: ... 7µL of RT-PCR mix (Superscript III RT/platinium Taq 0,1µL (Invitrogen), Reverse and Forward primers and spikes at 1,33µM final concentration and homemade 2X reaction Mix (120mM Tris SO4 pH=9 ...
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bioRxiv - Genomics 2020Quote: ... Standard quantitative RT-PCR (TaqPath 1-step RT qPCR Master Mix, Thermofisher) was run with test RNA and gene-specific standard curves (2e5 copy/mL – 20 copy/mL) ...
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bioRxiv - Molecular Biology 2020Quote: ... The cDNA products were analyzed on a StepOnePlus Real-time PCR system using SYBR Select Master Mix (Applied Biosystems). The primers used for different target genes are ...
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bioRxiv - Cancer Biology 2021Quote: The on-target indel frequencies were quantified using QuantStudio™ 12K Flex Real-time PCR System (Thermo Fisher Scientific) with Power SYBR Green Master Mix (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative polymerase chain reaction was performed with a QuantStudio 3 Real-Time PCR instrument (Thermo Fisher Scientific, Waltham, MA) using SYBR Green detection chemistry ...
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bioRxiv - Molecular Biology 2019Quote: ... functional primers with the corresponding probes were tested by qPCR using a StepOne Real-Time PCR instrument (Applied Biosystems). The following reagents were added for a 20 μL reaction mixture ...
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bioRxiv - Molecular Biology 2020Quote: ... Nick primers were used with SYBR® Green on the Step One plus Real-time PCR system (Applied Biosystems) and normalized to the Ct values obtained within the surrounding ∼300 bp DNA region amplified with the flanking primers (Table S2) ...
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bioRxiv - Cancer Biology 2021Quote: ... Real-time PCR (qPCR) was caried out using IQ-SYBR Green in a Step One Thermal Cycler (Applied Biosystems). Specific primers used are indicated in Supplementary Table 2 ...
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bioRxiv - Cell Biology 2020Quote: ... on a Step One Plus Real-Time PCR thermal cycler (Step One software v2.2.2; Applied Biosystems, Thermo Fisher Scientific). The Taqman gene expression assays for the reference gene (GAPDH – ID# Hs02786624_g1 ...
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bioRxiv - Cell Biology 2020Quote: ... on a Step One Plus Real-Time PCR thermal cycler (Step One software v2.2.2; Applied Biosystems, Thermo Fisher Scientific). The Taqman gene expression assays for the reference gene (GAPDH – ID# Hs02786624_g1 ...
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bioRxiv - Cell Biology 2020Quote: Transfection efficiency and altered gene expression were analysed using SBYR Green Real-Time PCR Master Mix (A25741; Applied biosystems). Briefly total RNA was extracted from cells using TRIzol (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of each cDNA were analyzed by real-time PCR with specific primers and PowerUp SYBR Green (Invitrogen) in an ABI QuantiStudio 3 real-time PCR system ...
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bioRxiv - Immunology 2021Quote: ... 2020) and reactions were carried out using a QuantStudio 6 and 7 Flex Real-Time PCR system (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... according to the manufacturer’s instruction and quantitative real-time PCR was performed using Taqman Gene expression assays (Applied Biosystems) to analyze expression of SARM1 (Rn01750947_m1) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Libraries were quantified using a ViiA™ 7 Real-Time PCR System (Applied Biosystems Corp., Foster City, CA, USA). Libraries were then pooled and diluted to 4 nM and submitted for sequencing.