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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for SARS CoV 2 Spike N Terminal Domain NTD Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: HEK293T cells were transferred with C-terminal HA-tagged Kayak and N-terminal GFP-tagged Jun-epi or Kayak using Lipofectamine 2000 (11668030, Thermo Fisher). The GFP-trap agarose beads (ABIN509397 ...
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bioRxiv - Immunology 2020Quote: The RBD domain of Spike (RBD, residues R319-S591) was transiently transfected into suspension-adapted ExpiCHO cells (Thermo Fisher) with PEI MAX (Polysciences ...
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bioRxiv - Microbiology 2019Quote: ... each fused to a C-terminal HA-tag using lipofectamine 3000 transfection reagent (ThermoFisher, L3000015) according to manufacturer instructions ...
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bioRxiv - Microbiology 2020Quote: ... incorporating amino acids 1–1,273 and a carboxyl-terminal trimerization domain was produced in Chinese Hamster Ovary cells (expiCHO; ThermoFisher) and purified by immunoaffinity chromatography ...
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bioRxiv - Molecular Biology 2023Quote: ... 25 wt% N,N,N’,N’-tetrakis(2-hydroxypropyl) ethylenediamine (Fisher Scientific, AAL16280AP) and 15 wt% Triton X-100 ...
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bioRxiv - Microbiology 2020Quote: Human FAPP1 cDNA was PCR-amplified from the HeLa cell total mRNA and cloned into pcDNA-6.2/N-EmGFP-DEST (for N-terminal tagging) by using the Gateway (Invitrogen) cloning technology as described previously [54] ...
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bioRxiv - Neuroscience 2022Quote: ... VGAT N-terminal antibody (rabbit polyclonal 131002, Invitrogen, Eugene, Oregon, USA; 1:1000); secondary antibody ...
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bioRxiv - Microbiology 2020Quote: ... The qRT-PCR assays were performed to quantify the amount of SARS-CoV-2 RNA with QuantStudio 3 instrument (Thermo Fisher Scientific, Waltham, MA, USA) using primers and probes described previously8,9 (N2 set or E set).
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bioRxiv - Microbiology 2020Quote: ... and SARS-CoV-2 RdRp IP2-targeted RdRp Institute Pasteur RT-PCR was performed on a QuantStudio™ 5 System (Applied Biosystems, Thermo Fisher Scientific). Log10 reduction values (LRV ...
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bioRxiv - Molecular Biology 2020Quote: HeLa cells (ATCC CCL-2) and HEK293-FT (ThermoFisher) were cultured at 37 °C in 5% CO2 in medium composed of high glucose Dulbecco’s modified eagles medium (DMEM ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μM DDR1 (Fisher Scientific, discoidin domain receptor 1 inhibitor). Time lapse images were acquired every 10 minutes for 24 hours.
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bioRxiv - Immunology 2021Quote: ... and 1% HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid; Gibco). Wild type SARS-CoV-2 (isolate USA-WA1/2020) ...
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bioRxiv - Immunology 2022Quote: ... 20 mM N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES, Gibco) and 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2022Quote: ... and 1% HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid; Gibco). Wild type SARS-CoV-2 (isolate USA-WA1/2020 ...
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bioRxiv - Genomics 2024Quote: ... and 2 μL of Dynabeads M-280 magnetic beads (Sheep Anti-Mouse IgG, Sheep Anti-Rabbit IgG, Invitrogen) using the SX-8G IP-Star Automated System (Diagenode) ...
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bioRxiv - Neuroscience 2021Quote: ... N-2 (Life Technologies), and B-27 supplement (Life Technologies)] ...
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bioRxiv - Systems Biology 2021Quote: ... 1X N-2 (Gibco), 1X B-27 minus vitamin A (Gibco) ...
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bioRxiv - Neuroscience 2021Quote: ... N-2 supplement (Gibco), Glutamax (Gibco) ...
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bioRxiv - Genetics 2019Quote: ... 1X N-2 (Gibco) and 10 U/ml Penicillin/Streptomycin (Gibco) ...
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bioRxiv - Genetics 2019Quote: ... 1X N-2 (Gibco), 10 ng/ml recombinant human FGF (Gibco) ...
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bioRxiv - Neuroscience 2022Quote: ... and N-2 (Gibco) (Neuronal Media) ...
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bioRxiv - Neuroscience 2023Quote: ... 1X N-2 (Gibco), 10 ng/ml bFGF (Life Technologies) ...
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bioRxiv - Bioengineering 2022Quote: ... 1% N-2 (Thermofisher), 2% B-27 (Thermofisher ...
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bioRxiv - Genomics 2022Quote: ... N-2 (17502048, Gibco), DKK1 (778606 ...
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bioRxiv - Genomics 2022Quote: ... N-2 (17502048, Gibco), BDNF (450-02 ...
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bioRxiv - Genetics 2023Quote: ... N-2 supplement (Gibco), D-Glucose (Sigma) ...
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bioRxiv - Genomics 2023Quote: ... 1X N-2 (Invitrogen), 1X B-27 (Invitrogen) ...
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bioRxiv - Genomics 2022Quote: ... N-2 (17502048, Gibco), BDNF (450-02 ...
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bioRxiv - Genomics 2022Quote: ... N-2 (17502048, Gibco) and 20 ng/ml bFGF (713304 ...
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bioRxiv - Neuroscience 2023Quote: ... 1x N-2 (Gibco), 10 U/ml Penicillin-Streptomycin (Gibco) ...
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bioRxiv - Neuroscience 2023Quote: ... 1x N-2 (Gibco) and 10 U/ml Penicillin-Streptomycin (Gibco) ...
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bioRxiv - Microbiology 2023Quote: ... 1× N-2 (Gibco), 50 ng/ml of mouse EGF (RnD) ...
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bioRxiv - Biochemistry 2019Quote: HelQ and the N-terminal HelQ fragment (N-HelQ) were over expressed using the Bac-to-Bac baculovirus expression system from Gibco BRL in SF9 insect cells ...
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bioRxiv - Systems Biology 2022Quote: ... ERCC synthetic spike-in 1 or 2 (Thermo Fisher Scientific Inc. ...
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bioRxiv - Systems Biology 2022Quote: ... ERCC synthetic spike-in 1 or 2 (Thermo Fisher Scientific Inc. ...
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bioRxiv - Cell Biology 2023Quote: ... 1:2×106 diluted ERCC spike-in RNAs (Ambion)] ...
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bioRxiv - Plant Biology 2019Quote: ... or pDEST-24 carrying a C-terminal GST purification tag (Thermo Fisher Scientific, Hampton, NH, USA).
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bioRxiv - Microbiology 2022Quote: ... The protein with a C-terminal Strep-tag was expressed in Expi293 cells (Thermo Fisher Scientific) and subsequently purified using Strep-Tactin XT Superflow high capacity resin (IBA Lifesciences ...
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bioRxiv - Molecular Biology 2021Quote: Mimivirus L375 appended with a C-terminal 10X histidine tag was generated by GeneArt (Life Technologies) for codon-optimized expression in Escherichia coli ...
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bioRxiv - Microbiology 2020Quote: ... Bound protein was detected via the C-terminal 6XHis Tag using 6XHis Antibody MA1-135 (Invitrogen) followed by three washes in TBS-T and incubation with donkey anti-mouse horseradish peroxidase (HRP)-coupled secondary antibody (Dianova) ...
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bioRxiv - Microbiology 2021Quote: ... NL63-CoV and the N-terminal peptidase domain of wild-type and human ACE2 SNVs were expressed using the Bac-to-Bac baculovirus system (Invitrogen). Specifically ...
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bioRxiv - Microbiology 2021Quote: ... Cys469 in the C-terminal DNase domain of these ColE9 constructs was labelled with a 3-fold Alexa Fluor 488-maleimide (Invitrogen) as previously described (41) ...
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bioRxiv - Molecular Biology 2023Quote: ... The SARS-CoV-2 nucleocapsid gene was reverse transcribed and amplified using the TaqMan® RNA-to CT™1-Step Kit (ThermoFisher Scientific, Waltham, MA, USA), using Forward primers ...
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFUSE-vector (Invitrogen, Thermo Fischer Scientific, MA, USA). The human ACE2 was expressed by essentially the same protocol used for the COVID-19 RBD ...
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bioRxiv - Immunology 2022Quote: ... B.1.617.2 (delta) and B.1.1.529 (Omicron)] and MERS-CoV spike using Lipofectamine 2000 (ThermoFisher Scientific cat.# 11668019) to produce single-round infection-competent pseudoviruses (80) ...
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bioRxiv - Microbiology 2021Quote: ... Gibco Hepes (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid, 0.05 mM; Invitrogen), ROCK Inhibitor Y-27632 (1 mM ...
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bioRxiv - Neuroscience 2023Quote: ... 5mM N-2-hydroxyethyl piperazine-N-2-ethane sulfonic acid (HEPES; Gibco, 15630080), and cOmplete ...
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bioRxiv - Neuroscience 2021Quote: ... and modified with a N-terminal signalling peptide from the commercial pDisplay vector (Invitrogen) and a trafficking signal prior to tdTomato or citrine fusion and inserted in the pcDNA3.1+ vector (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... The protein with the C-terminal Fc-fusion was affinity purified with protein-A sepharose (Invitrogen). Samples were eluted with 0.1 M glycine pH 3.0 directly to a neutralizing buffer ...
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bioRxiv - Immunology 2022Quote: Expression plasmids encoding His-tagged SARS-CoV-2 RBD (residues 319 to 541 of SARS-CoV-2 S protein) or S protein (with a C-terminal trimerization domain) were cloned into pCEP4 vector and transfected into Expi293F (ThermoFisher Scientific) and the proteins expressed for 7 days at 37°C (Rouet et al. ...