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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for SARS CoV 2 Spike N Terminal Domain NTD Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 11 cDNA fragments with 70 bp end-terminal overlaps which spanned the entire SARS-CoV-2 isolate Wuhan-Hu-1 genome (GenBank accession: NC_045512) were produced by GeneArt™ synthesis (Invitrogen™, ThermoFisher) as inserts in sequence verified ...
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bioRxiv - Immunology 2020Quote: ... SARS-COV-2 Spike-specific F(ab’)2 was isolated by affinity chromatography using recombinant antigen (1 mg SARS-CoV-2 S-2P or RBD) coupled to 0.05 mg dry NHS-activated agarose resin (Thermo Fisher Scientific) as follows ...
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bioRxiv - Genomics 2021Quote: ... standard PCR-based detection of SARS-CoV-2 was initiated by extraction of viral RNA from nasopharyngeal (NP) swabs (KingFisher™ Flex Magnetic Particle Processor, ThermoFisher). The viral RNA was analyzed ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells (PBMCs) from SARS-CoV-2 convalescent donors were stained with Live/Dead Fixable Aqua (Invitrogen; Thermo Scientific) in 100 μL final volume diluted 1:500 at room temperature (RT) ...
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bioRxiv - Microbiology 2021Quote: Fifty microliters containing 3.5 x 106 PFU/ml SARS-CoV-2 viral stock was placed on one well of a 4-well chambered glass slide (Nunc, Sigma) (Fig 2) ...
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bioRxiv - Immunology 2021Quote: ... Protein labelled cells were stained with biotinylated SARS-CoV-2 S1 (ACRO biosystems – S1N-C82E8) and detected with streptavidin AF647 (Invitrogen – S21374). Cells were stained with 7-AAD viability staining solution at 5 μl/1×106 cells to determine live cells ...
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bioRxiv - Microbiology 2022Quote: ... Calu3 monolayers were washed once with MEM-Eagles medium (MEM) without FBS and infected with SARS-CoV-2 in the presence of 20 μg per ml TPCK trypsin (Thermo scientific). Plates were incubated for 1 h at 37°C to allow viral adsorption ...
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bioRxiv - Microbiology 2022Quote: Copies of SARS-CoV-2 E gene (subgenomic) were measured by qRT-PCR with TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes sgLeadSARSCoV2-F 5’-CGATCTCTTGTAGATCTGTTCTC-3’ ...
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bioRxiv - Biochemistry 2022Quote: ... The Z-scores for reactivity of SARS CoV-2 proteins to each human protein was determined by using ProtoArray Prospector v 5.2 software (Invitrogen, Thermo Fisher).
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2/UT-NCGM02/Human/2020 (Wuhan strain) was propagated in VeroE6/TMPRSS2 cells in VP-SFM (Thermo Fisher Scientific).
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bioRxiv - Immunology 2023Quote: ... HIV reporter pseudoviruses expressing SARS-CoV-2 S glycoprotein and carrying the luciferase gene were produced in Expi293F cells (ThermoFisher Scientific) by co-transfection of the pNL4-3 ...
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bioRxiv - Microbiology 2023Quote: ... or pCDNA SARS-CoV-2 ORF7a-V5/His (WT/H47Y; 0.75 μg) or empty vector using Lipofectamine 3000 (Thermo Fisher Scientific) per manufacturer’s recommendation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 50 μL of a 1:4,000 dilution of rabbit anti-SARS-CoV-2 nucleocapsid antibody (Invitrogen, Waltham, MA, Cat# MA536086) in blocking buffer was added and incubated for 2 h at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... 5μg of the infectious clone and 100ng of a SARS-CoV-2 WA-1 nucleoprotein expression plasmid were diluted in 100μL of OptiMEM (Gibco, Waltham, MA). 3μL of TransIT-2020 (Mirus Bio ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293 cells were used as host cells to transfect the SARS-CoV-2 FSE dual luciferase reporter plasmid using Lipofectamine 2000 transfection reagent (ThermoFisher Scientific). The -1 PRF efficiency was assayed in cultured HEK293 as described previously (Kelly et al ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were probed with rabbit-anti-SARS spike (Invitrogen, PA1-411-1165, 0.5ug/ml), rabbit-anti-Orf6 (Abnova ...
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bioRxiv - Microbiology 2021Quote: ... pCG1-SARS-2-SdC19 or pCG1-SARS-2-SdC19-H2 using Lipofectamine 2000 (ThermoFisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... recombinant SARS-CoV-2 Spike RBD protein (RBD, His Tag) was conjugated with EZ-LinkTM Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Immunology 2021Quote: ... or for intracellular expression using the pYES2/NTC vector (N-terminal Xpress and C-terminal V5 epitope, Invitrogen). The pYD1 system used the yeast internal Apa1/Ap2 system ...
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bioRxiv - Microbiology 2022Quote: ... was synthesized as a custom peptide with N-terminal acetylation and C-terminal amidation (Life Technologies Europe Bv).
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bioRxiv - Immunology 2021Quote: The SARS-CoV-2 receptor-binding domain (RBDwt) and the RBD mutant (RBDmut) (K417N, E484K, N501Y) were expressed using Expi293F cells (Invitrogen, ThermoFisher Scientific, MA, USA). The genes that encode SARS-CoV-2 RBDwt (residues Arg319-Phe541 ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from mock and SARS-CoV-2 infected proximal airway ALI and alveolar organoid cultures lysed in Trizol (Thermo Fisher Scientific) using the chloroform-iso-propanol-ethanol method ...
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bioRxiv - Immunology 2021Quote: ... and searched against the UniProt Human protein database and RefSeq SARS-CoV-2 protein database using Sequest HT through Proteome Discoverer (Version 2.2) (Thermo Scientific, Bremen, Germany). Precursor and fragment mass tolerance were set to 10 ppm and 0.02 Da ...
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bioRxiv - Microbiology 2020Quote: The melting profiles were obtained incubating 10 ng of SARS-CoV-2 RNA with 10 or 100nM of DCV and Sybergreen (1x) (Thermo Fisher Scientific) in an StepOne™ Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells (PBMCs) from SARS-CoV-2 convalescent donors were stained with Live/Dead Fixable Aqua (Invitrogen; Thermo Scientific) in 100 μL final volume diluted 1:500 at room temperature (RT) ...
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bioRxiv - Bioengineering 2021Quote: SARS-CoV-2 S was chemically biotinylated using EZ-Link™ NHS-LC-LC-Biotin following manufacturer instructions (Thermo Fisher, Cat#21343). Vero E6 cells (ATCC ...
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bioRxiv - Microbiology 2021Quote: ... and the SARS-CoV-2 isolate Wuhan-Hu-1 (GenBank accession #: MN908947) at a 4:1 ratio by lipofectamine 3000 (Thermo Fisher Scientific). After 48 to 72 hours ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 RDB protein was also coupled to DyLight 650 using the DyLight® Amine-Reactive Dyes kit (Thermo Fisher scientific).
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bioRxiv - Microbiology 2022Quote: ... The viral genome between 27,895-29,534 nucleotides based on the SARS-CoV-2 WA-1 strain was RT-PCR amplified using Super Script II Reverse transcriptase (Thermo Fisher Scientific) and Expanded High Fidelity PCR system (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: mRNAs were extracted from neurons derived from AD or Control hiPSCs with or without SARS-CoV-2 infection in Trizol reagent (Thermofisher, Waltham, MA) and followed by cDNA synthesis with SuperScript™ III Reverse Transcriptase kit (ThermoFisher) ...
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bioRxiv - Immunology 2021Quote: ... 10,000 Hela-hACE2 cells generated by lentivirus transduction of wild-type Hela cells and enriched by fluorescence-activated cell sorting (FACS) using biotinylated SARS-CoV-2 RBD conjugated with streptavidin-Alexa Fluor 647 (ThermoFisher Scientific, S32357) were added to the mixture with 20 µg/ml Dextran (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cells were infected with 0.1 MOI of SARS-CoV-2 using Opti-MEM I reduced serum medium (Thermo Fisher, Cat#31985062) and incubated for 1 hour at 37 °C with 5% CO2 ...
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bioRxiv - Molecular Biology 2021Quote: The SARS-CoV-2 RdRp partial RNA (see Supplementary Table S2) were in vitro transcribed using T7 RNA polymerase (Thermo Fisher Scientific) and synthetic DNA templates carrying a T7 promoter sequence ...
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bioRxiv - Microbiology 2021Quote: ... RT-PCR amplification of the viral genome spanning nucleotides 27,895-29,534 (according to the SARS-CoV-2 USA-WA1/2020 viral genome) was performed using Super Script II Reverse transcriptase (Thermo Fisher Scientific) and Expanded High Fidelity PCR System (Sigma Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... half the wells exposed to particulate and half the control wells were apically infected with SARS-CoV-2 derived from clinical isolate WA1 (20) in high glucose DMEM (Gibco 11995-065) with 5% heat-inactivated fetal bovine serum ...
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bioRxiv - Microbiology 2021Quote: ... 11 cDNA fragments with 70 bp end-terminal overlaps which spanned the entire SARS-CoV-2 isolate Wuhan-Hu-1 genome (GenBank accession: NC_045512) were produced by GeneArt™ synthesis (Invitrogen™, ThermoFisher) as inserts in sequence verified ...
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bioRxiv - Microbiology 2021Quote: ... Cells were incubated with mouse mAb SARS-CoV-2 nucleocapsid antibody (SinoBiological, 1:100) and rabbit Claudin 7 polyclonal antibody (ThermoFisher, 1:200) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells (PBMCs) from SARS-CoV-2 convalescent donors were stained with Live/Dead Fixable Aqua (Invitrogen; Thermo Scientific) in 100 μL final volume diluted 1:500 at room temperature (RT) ...
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bioRxiv - Genomics 2021Quote: PCR amplification of SARS-CoV-2 genome was carried out using AmpliTaq Gold 360 Master Mix (Applied Biosystems, Foster City, California, USA) with reaction constituents as 1X 360 master mix ...
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bioRxiv - Immunology 2022Quote: ... Batches were sequenced using the Ion AmpliSeq SARS-CoV-2 Insight Research Assay on an Ion Torrent Genexus Integrated Sequencer (Thermo Fisher Scientific). Each run completed within 24h ...
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bioRxiv - Immunology 2023Quote: ... Extraction of SARS-CoV-2 RNA was performed using the MagMax Viral/Pathogen Nucleic Acid Isolation kit (Thermo Fisher Scientific, Waltham, MA) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... the transfected cells were harvested and stained with Alexa Fluor 647-anti-SARS-CoV-2 RBD antibody (51-6490-82; Thermo Fisher Scientific). Ancestral and variant spike expressing HEK293T cells were sorted for as target cells using the FACSAria™ Fusion Cell Sorter (BD Biosciences ...
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bioRxiv - Microbiology 2023Quote: SARS-CoV-2 Nucleocapsid antibody (produced in mouse) as a primary antibody was procured from (MA5-35943, Invitrogen, 1:1000-1:10,000), Rabbit phospho-p38 MAPK and total p38 MAPK Antibody were procured from (9211S and 9212S ...
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bioRxiv - Microbiology 2023Quote: ... the VSV antigenome without the G gene and the SARS-CoV-2 S gene were amplified by PCR using Platinum™ SuperFi II PCR Master Mix (Thermo Fisher). PCR products were gel-purified and assembled using NEBuilder® HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Immunology 2023Quote: ... For hamster samples a SARS-CoV-2 Nucleocapsid antibody (SinoBiological #40588) followed by a goat anti-rabbit IgG Alexa Fluor 488 antibody (ThermoFisher Scientific #A32731) was used (6) ...
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bioRxiv - Immunology 2023Quote: ... Results of the human samples were developed using a SARS-CoV-2 convalescent plasma as primary and a goat anti-human IgG Alexa Fluor 488 secondary antibody (ThermoFisher Scientific #A48276) (2) ...
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bioRxiv - Bioengineering 2024Quote: We conducted quality control checks of bead quality prior to cartridge loading with synthetic template standards of SARS-CoV-2 RNA (TWIST #102024) diluted in UltraPure™ DNase/RNase-free distilled water (UPdH2O - Invitrogen #10977015). To evaluate the performance of the NABIT reaction system ...
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bioRxiv - Microbiology 2024Quote: ... were transfected with the plasmid encoding the stabilized SARS-CoV-2 soluble S protein with the Expifectamine kit (GIBCO, Waltham, MA, USA) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Codon-optimized gene encoding the full-length SARS-CoV-2 PLpro (1-315 amino acids) was custom synthesized (Invitrogen, Thermo Fisher Scientific) and was cloned using XhoI and NdeI restriction sites in pET28c vector containing an N-terminal 6X histidine tag ...
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bioRxiv - Molecular Biology 2023Quote: ... Codon-optimized gene encoding the full-length SARS-CoV-2 PLpro (1-315 amino acids) was custom synthesized (Invitrogen, Thermo Fisher Scientific) and was cloned using XhoI and NdeI restriction sites in pET28c vector containing an N-terminal 6X histidine tag ...