Labshake search
Citations for Thermo Fisher :
401 - 450 of 10000+ citations for SARS CoV 2 Spike N Terminal Domain NTD Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... were cultured in the presence of SARS-CoV-2-S (9pmol) (PepTivator) in 24-well plates and maintained in IMDM medium (Gibco) supplemented with penicillin (100 units/mL) ...
-
bioRxiv - Genetics 2022Quote: ... Genotyping was done using Axiom™ Human Genotyping SARS-CoV-2 Research Array as instructed by the manufacturer (Thermo Scientific).
-
bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 D614G S ectodomain was produced in Expi293F cells grown in suspension using Expi293 expression medium (Life Technologies) at 37°C in a humidified 8% CO2 incubator rotating at 130 rpm ...
-
bioRxiv - Genomics 2020Quote: ... 250,000 or 1000 SARS-CoV-2 viral copies were used to start the initial cDNA synthesis by SuperScript III reverse transcriptase (Invitrogen), using a mix of random primers ...
-
bioRxiv - Molecular Biology 2021Quote: The wild-type and mutant SARS-CoV-2 S glycoproteins were expressed transiently by a pcDNA3.1(-) vector (Thermo Fisher Scientific) (29) ...
-
bioRxiv - Microbiology 2021Quote: ... cells transfected with the SARS-CoV-2 S protein deletion mutants and controls were washed once with DPBS (Fisher Scientific), fixed with 4% (w/v ...
-
bioRxiv - Microbiology 2020Quote: Total RNA was extracted from the supernatants of SARS-CoV-2-infected cells by using a PureLink RNA Mini Kit (Invitrogen). Thereafter ...
-
bioRxiv - Microbiology 2021Quote: CHO cells stably expressing SARS-CoV-2 S-glycoprotein were seeded in 96 well plates for microscopy (Thermo Fisher Scientific) at 12’500 cells/well and the following day ...
-
bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 viral nucleocapsid protein (NP) was detected using the anti-NP protein antibody (Cat. # PA5-81794, Thermo Fisher) diluted 1:10000 in 0.1% tween-20/1%BSA/PBS solution as a primary antibody ...
-
bioRxiv - Immunology 2020Quote: ... For neutralization assays HEK 293T expressing the SARS-CoV-2 receptor ACE2 (HEK 293T/ACE2) were cultured in DMEM (Gibco), supplemented with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Immunology 2020Quote: ... Cryopreserved PBMC from the SARS-CoV-2-infected participant and a SARS-naïve donor were thawed at 37°C and stained for SARS-CoV-2-specific memory B cells with a flow cytometry panel consisting of: a viability dye (7AAD, Invitrogen), CD14 PE-Cy5 ...
-
bioRxiv - Immunology 2020Quote: ... The detection and measurement of SARS-CoV-2 viral load were performed using COVID-19 RT-PCR diagnostic kit with specific Taqman probes (ThermoFisher) of SARS-CoV-2-specific genes encoded by the RNA-dependent RNA polymerase (RdRp/ORF1ab) ...
-
bioRxiv - Microbiology 2021Quote: ... assay using the OPTI Medical SARS-CoV-2 RT-PCR kit following the manufacturer’s instructions on an ABI 7500 Fast instrument (ThermoFisher Scientific). The OPTI Medical SARS-CoV-2 RT-PCR assay detects two different targets in the gene encoding viral nucleocapsid (N ...
-
bioRxiv - Microbiology 2020Quote: The wild-type and mutant SARS-CoV-2 S glycoproteins were expressed transiently by a pcDNA3.1(-) vector (Thermo Fisher Scientific). The wild-type SARS-CoV-2 spike (S ...
-
bioRxiv - Microbiology 2021Quote: ... Peptide identification of the human and SARS-CoV-2 proteome was performed by Proteome Discoverer (version 2.2, Thermo Fisher Scientific) using the SequestHT search engine as described (Seidel et al 2018) ...
-
bioRxiv - Microbiology 2022Quote: ... total RNA from non-infected (Ctrl) or SARS-CoV-2 infected A549-hACE2 and hPCLS was isolated using Trizol (Invitrogen). RNA was treated with DNase (DNase-Free DNase Set ...
-
bioRxiv - Microbiology 2022Quote: ... were transfected into Calu-3 cells using Dharmafect-4 transfection reagent (#cat: T-2004-02, Dharmacon) 48 hours prior to infection with SARS-CoV-2: siAAK1 (ID: s22494, Ambion), siBIKE (M-005071-01 ...
-
bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 targeting probes were custom-made #6007037-01 directed against the ORF1a sequences between positions 401-1327 (ThermoFisher). Subsequently ...
-
bioRxiv - Immunology 2022Quote: All SARS-CoV-2 S ectodomains were produced in 500-mL cultures of FreeStyle™ 293-F cells (ThermoFisher Scientific) grown in suspension using FreeStyle 293 expression medium (ThermoFisher Scientific ...
-
bioRxiv - Biochemistry 2022Quote: ... The Z-scores for reactivity of SARS CoV-2 proteins to each human protein was determined by using ProtoArray Prospector v 5.2 software (Invitrogen, Thermo Fisher).
-
bioRxiv - Immunology 2023Quote: The SARS-CoV-2 pseudovirus-based neutralization assay were performed in Nunc 96-well cell culture plates (Thermo Fisher Scientific). Briefly ...
-
bioRxiv - Neuroscience 2023Quote: Neurons from K18-hACE2 and WT mice were inoculated with SARS-CoV-2 isolate USA-WA1/2020 at 30 MOI in 100 µL Neurobasal A (Invitrogen) for 8-well chamber slides and 200 µL for 24-well plates for 1 h ...
-
bioRxiv - Microbiology 2023Quote: ... Six- to 12-week-old male and female mice were inoculated by the intranasal route with 103 pfu of SARS-CoV-2 in a volume of 50ul PBS (Gibco). Prior to inoculation mice were anesthetized by inhalation of isoflurane ...
-
bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 Nucleocapsid antibody (produced in mouse) was procured from (MA5-35943/Invitrogen/Massachusetts, USA/1:1000-1:10,000) used as primary antibody ...
-
bioRxiv - Immunology 2023Quote: Complement sufficient NHS (0-10% final concentration) was mixed 103 PFU of SARS-CoV-2 and RNAse A (1mg/ml,Thermofisher) for 2 hr at 37°C ...
-
A Drug-Free Pathogen Capture and Neutralizing Nasal Spray to Prevent Emerging Respiratory InfectionsbioRxiv - Bioengineering 2023Quote: ... alcohol solution or PBS was mixed with 8×104 PFU of SARS-CoV-2 mNG in 50 uL of infection media (OptiMEM (Gibco) containing 3 μg/mL TPCK-trypsin) ...
-
bioRxiv - Immunology 2023Quote: Affinity purified SARS-CoV-2 (Wuhan) RBD was biotinylated using EZ-Link Sulfo-NHS-LC-biotin (Life Technologies, USA, A39257) accordingly to manufacturing protocol ...
-
bioRxiv - Immunology 2024Quote: ... Serum serial dilutions were incubated with 2000 PFU of an early SARS-CoV-2 isolate in 100 µl OptiPro medium supplemented with 1x GlutaMAX (both Gibco) for 1 h at 37 °C ...
-
bioRxiv - Bioengineering 2022Quote: ... a C-terminal gp160 trimerization domain and SnoopTagJr) were expressed in suspension Expi293F (Thermo Fisher) and ExpiCHO-S cells respectively and purified as described above for DogCatcher-RBD.
-
bioRxiv - Biochemistry 2022Quote: ... RBD of SARS-CoV2 and SARS-CoV S were transiently expressed in FreeStyle™ 293-F cells (Thermo Fisher Scientific). Cells were resuspended at 1×106 per mL with fresh media on the day of transfection ...
-
bioRxiv - Immunology 2022Quote: ... 2% FCS (ThermoFisher), and 50 μg/mL gentamicin (ThermoFisher) ...
-
bioRxiv - Immunology 2023Quote: ... 2% FCS (Gibco), 25 mM HEPES (Merck ...
-
bioRxiv - Microbiology 2020Quote: ... Rabbit anti-SARS-CoV nucleocapsid polyclonal antibody (PA1-41098, Thermo Scientific, Rockford, IL) diluted 1:2,000 in blocking buffer was allowed to bind overnight at 4°C ...
-
bioRxiv - Immunology 2021Quote: Cells exposed to SARS-CoV-2 pseudovirus were lysed and mRNA was isolated with the mRNA Catcher™ PLUS Purification Kit (ThermoFisher). Subsequently ...
-
bioRxiv - Immunology 2022Quote: ... the SARS-CoV-2-TCR mRNA was transcribed in vitro using the mMESSAGE mMACHINE™ T7 ULTRA Transcription Kit (ThermoFisher Scientitic) following the manufacturer’s protocols ...
-
bioRxiv - Microbiology 2020Quote: ... washed twice with PBS and stained with anti-SARS-CoV-2 (1A9; Biozol GTX-GTX632604) anti-MUC5AC (clone 45M1; MA1-21907, Thermo Scientific) and anti-alpha-tubulin (MA1-8007 ...
-
bioRxiv - Microbiology 2020Quote: ... RT-qPCR (SARS-CoV-2 and MS2 viral genome detection) were performed with the Express one step RT-qPCR Universal kit (ThermoFisher Scientific) using 3.5µL of RNA and 6.5µL of RT-qPCR mix that contains 250nmol of each primer and 75nmol of probe ...
-
bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 RNA levels were measured by quantitative PCR using TaqMan Fast Universal PCR Master Mix (Thermo Fisher: Cat# 4352042) on the Applied Biosystems 7500 Fast Dx Real-Time PCR System ...
-
bioRxiv - Biochemistry 2021Quote: The SARS-CoV-2 Omicron HexaPro S protein gene was synthesized and inserted into pcDNA3.1 (GeneArt Gene Synthesis, Thermo Fisher Scientific).
-
bioRxiv - Microbiology 2020Quote: ... Viral RNA levels were quantified by quantitative reverse-transcriptase PCR (qRT-PCR) specific for the SARS-CoV-2 E gene (23) using TaqMan Fast 1-Step Master Mix (Applied Biosystems) on a 7500 Fast Real-Time PCR System (Applied Biosystems) ...
-
bioRxiv - Molecular Biology 2020Quote: ... SARS-CoV-2 S trimer protein was biotinylated using the EZ-Link(tm) Sulfo-NHS-LC-LC-Biotin kit (Thermo Fisher) and then purified using Zeba(tm ...
-
bioRxiv - Cell Biology 2022Quote: ... Co-cultures were infected with 1x104 PFU of SARS-CoV-2 in 100 µl of OptiMEM (Thermo Fisher Scientific, Waltham, MA) added to the apical surface ...
-
bioRxiv - Microbiology 2022Quote: ... 5μg of the infectious clone and 100ng of a SARS-CoV-2 WA-1 nucleoprotein expression plasmid were diluted in 100μL of OptiMEM (Gibco, Waltham, MA). 3μL of TransIT-2020 (Mirus Bio ...
-
bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells (PBMCs) from SARS-CoV-2 convalescent donors were stained with Live/Dead Fixable Aqua (Invitrogen; Thermo Scientific) in 100 μL final volume diluted 1:500 at room temperature (RT) ...
-
bioRxiv - Microbiology 2021Quote: A mammalian expression plasmid expressing the coding sequence of SARS-Cov-2 Nsp4-Nsp5 in a pCDNA3.1 backbone was synthesized (GeneArt™ Gene synthesis, ThermoFisher, USA). HEK 293T cells in a 6 well plate were transfected with polyethylenimine (PEI ...
-
bioRxiv - Biochemistry 2020Quote: The cDNA coding for SARS CoV-2 Nsp1 1-180 Nsp2 1-19 8His was synthesised by GeneArt (Thermo Fisher Scientific) adding NcoI and NotI sites at the 5’ and 3’ ends ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: A codon-optimized gene encoding for SARS-CoV-2 (331 to 528 amino acids, QIS60558.1) was expressed in Expi293 cells (Thermo Fisher Scientific) with human serum albumin secretion signal sequence and fusion tags (6xHistidine tag ...
-
bioRxiv - Immunology 2022Quote: ... 30 μl of SARS-CoV-2 S-5P or 6P at 5 ng/µl were plated onto 384-well Nunc Maxisorp (Thermo Fisher) plates in PBS and sealed overnight at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... were transfected in suspension with 4.0 µg/well of SARS-CoV-2/mCherry-Nluc BAC plasmid using Lipofectamine 2000 (Thermo Fisher Scientific). Transfection media was changed to post-infection media (DMEM containing 2% FBS and 1% PSG ...
-
bioRxiv - Biochemistry 2021Quote: ... The recombinant poly-histidine tagged ectodomains of the SARS-CoV-2 S proteins (614D and 614G) were expressed in human Expi293F cells using the Expi293 Expression System (ThermoFisher Scientific) and purified using HisTrap FF column (GE Life Sciences)18 ...