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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for Glycerol Kinase 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Pooled peptides were fractionated by a high-pH reversed-phase peptide fractionation kit (84868, Thermo Fisher, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... The digested peptides were fractionated using a high-pH reversed phase (RP) peptide fractionation kit (Thermo Scientific). Fractions collected from the high-pH RP fractionation were further separated using on-line second-dimension low-pH reversed-phase chromatography via an Easy-nLC 1200 nanoflow-UHPLC system (Thermo Scientific ...
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bioRxiv - Biochemistry 2020Quote: Pooled peptides were fractionated using a high-pH reversed-phase peptide fractionation kit (84868, Thermo Fisher, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Peptide concentration was quantified with a plate reader using the Pierce Quantitative Colorimetric Peptide Assay (Thermo Scientific). Finally ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 25 fmol/µl standard peptide mixture (Pierce™ Peptide Retention Time Calibration Mixture, ThermoFisher, 88321) and diluted to a final volume of 23 µl with water ...
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bioRxiv - Cell Biology 2022Quote: ... The peptide concentration was measured using the Pierce™ Quantitative Colorimetric Peptide Assay (Thermo Scientific cat. # 23275). The digested peptides were subjected to simple C18 clean up using a C18 disk plug (3M Empore C18 ...
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bioRxiv - Neuroscience 2023Quote: ... desalted peptides was separated into 9 fractions with the high pH reversed-phase peptide fractionation kit (ThermoFisher). Fractions with increasing percentages of acetonitrile (ACN ...
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bioRxiv - Microbiology 2023Quote: ... the concentration of peptides was measured using Pierce Quantitative fluorometric peptide assay (ThermoFisher Scientific, Rockford, IL, USA).The resulting peptides were sequenced on a Q-Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The peptide concentration in the supernatant was quantified using a quantitative colorimetric peptide assay from Thermo Fisher Scientific (Cat ...
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bioRxiv - Pathology 2024Quote: ... The concentration of the peptides was determined using Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific, Cat#23275). Tandem mass tag (TMT)-based labeling of peptides was performed using the TMT10plex™ Isobaric Label Reagent Set (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... and the supernatant was recovered for peptide desalting using Pierce™ Peptide Desalting Spin Column (ThermoFisher: 89851) as described by the manufacturer ...
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bioRxiv - Microbiology 2024Quote: ... 0.1% TFA and peptide content was estimated with a Pierce™ Quantitative Colorimetric Peptide Assay (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... were used following biotin blocking of tissues samples using endogenous Biotin-blocking kit (Life Technologies Corporation/Thermo fisher, E21390). For Aza-peptide preparation ...
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bioRxiv - Molecular Biology 2021Quote: ... were used following biotin blocking of tissues samples using endogenous Biotin-blocking kit (Life Technologies Corporation/Thermo fisher, E21390). For Aza-peptide preparation ...
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bioRxiv - Cell Biology 2020Quote: ... and 1X PBS) before blocking for one hour with blocking buffer (Bovine Serum Albumin (Fisher Scientific, Fair Lawn, NJ), Normal Donkey Serum (Jackson ImmunoResearch Inc. ...
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bioRxiv - Physiology 2022Quote: ... Non-specific antigens were blocked for 60 min using a blocking buffer (Sea Block Blocking Buffer; ThermoFisher Scientific, USA). Then ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10 mM ATP in kinase buffer (20 mM Tris pH 8, 150 mM NaCl, 10 mM MgCl2, 1 mM DTT, 1X Phosphatase inhibitor (Thermo Scientific, #1862495)) at room temperature for 2 h ...
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bioRxiv - Molecular Biology 2023Quote: ... was labeled with 1 µL of [γ32P] ATP (3000 Ci/mmol, Hartman Analytic GmbH) and 10 U T4 polynucleotide kinase (Thermo Fisher Scientific) for 10 min at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Tubes had equal amounts of glycerol and 0.04 M phosphate buffer (pH 7.2–7.6) containing 1× antibiotic–antifungal mixture (Thermo-Fisher Scientific, Johannesburg, South Africa) (46,47) ...
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A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteinsbioRxiv - Molecular Biology 2020Quote: ... Cells were resuspended in buffer A (10 mM HEPES [pH7.9], 10 % glycerol, 1 mM DTT, protease inhibitor cocktail [Thermo Fisher Scientific]). After adding 0.1% Triton X-100 ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 μL of magnetic HA beads was washed twice in 1X PBS with 0.1% Tween 20 and then twice in binding buffer (50 mM Tris pH7.5, 150 mM NaCl, 1% IGEPAL CA-630, 5% glycerol, and protease inhibitors (Thermo Fisher #A32955): apoptin ...
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bioRxiv - Genomics 2020Quote: ... 1% Triton X-100 and 10% glycerol and resolved on 3–8% pre-cast gradient Tris-Acetate gels (Invitrogen, Waltham, MA). For mouse samples ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7.5/ EDTA 2 mM/glycerol 10%/Triton X-100 1%/sodium deoxycholate 0.5%/SDS 0.2%) supplemented with protease inhibitor cocktail (Thermo Fisher Scientific, A32965) from 10-cm dishes ...
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bioRxiv - Molecular Biology 2022Quote: ... were washed three times with Buffer A (PBS pH 8.3, 5% glycerol, 1 mM DTT, 0.03% NP-40, Pierce™ Phosphatase Inhibitor Mini Tablets (Thermo Fisher Scientific)) and incubated with 2 µg of un ...
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bioRxiv - Molecular Biology 2022Quote: ... streptavidin particles with immobilized peptides were washed three times with buffer B (PBS pH 8.3, 5% glycerol, 1 mM DTT, 0.1% NP-40, Pierce™ Phosphatase Inhibitor Mini Tablets (Thermo Fisher Scientific), Roche Complete Mini EDTA-free protease inhibitor tablets (Sigma-Aldrich ...
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bioRxiv - Physiology 2022Quote: ... washed once with cold acetone and then dissolved in Laemmli buffer (Tris 10 mM pH 7.5, EDTA 1 mM [Fluka, Buchs, Switzerland], β-mercaptoethanol 5%, SDS 5%, glycerol 10% [ThermoFisher Scientific]). Sonication and centrifugation were repeated as above to pellet and eliminate possibly remaining cell debris ...
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bioRxiv - Bioengineering 2021Quote: Inoculated starter cultures of 6mL LB-kanamycin that were shaker incubated at 37C 250rpm overnight were used to make 25% glycerol stocks and inoculate in a 1:50 ratio TB-kanamycin in baffled 1L flasks (Thermo Fisher). Cultures were shaker incubated at 37C 250rpm until OD600 (Nanodrop ...
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Metal transporter SLC39A14/ZIP14 modulates regulation between the gut microbiome and host metabolismbioRxiv - Physiology 2021Quote: ... the pellets were resuspended in lysis buffer (Tris-HCl, 137mM NaCl, 10% glycerol, 1% Triton X-100, 2 mM EDTA) with protease and phosphatase inhibitors (Thermo Scientific) added along with the PMSF (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2024Quote: ... 200 mM NaCl, 0.08% Triton X-100, 25% glycerol, 20 mM reduced glutathione, 1 mM DTT, and 1X synthetic block [Invitrogen cat# PA017]) for one hour with gentle rocking ...
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bioRxiv - Neuroscience 2019Quote: ... and treated with 10 μM of freshly prepared amyloid-β peptide 1-42 (Aβ42, Life technologies) for 24 h ...
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bioRxiv - Neuroscience 2019Quote: ... or 10 μM of freshly prepared human synthetic amyloid-β peptide 1-42 (Aβ42, Life technologies) for 24 h as described (Kiyota et al. ...
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bioRxiv - Developmental Biology 2021Quote: 1 µg of the peptide samples were injected into an Ultimate 3000 RSLC system (Thermo Scientific) connected to a Q-Exactive HF mass spectrometer (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: Peptides (1 µg/run) were separated with an EASY-nLC 1000 nanoflow liquid chromatograph (Thermo Fisher) on a 75 µm diameter ...
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bioRxiv - Microbiology 2022Quote: ... and then elution was performed with 1 mg/mL HA synthetic peptide (Cat# 26184, Thermo Fisher) per the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... 1 µg of peptides was injected into a reverse phase column (EASY-SprayTM - ES902 - Thermo Scientific: 25cm x 75 µm ID ...
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bioRxiv - Biophysics 2023Quote: ... peptides were loaded onto a C18 analytical column (1 mm i.d. × 50 mm, Thermo Fisher Scientific) connected to an Agilent 1290 Infinity II UHPLC system ...
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bioRxiv - Neuroscience 2020Quote: ... then nutated in blocking solution with secondary antibody Streptavidin Alexafluor conjugate 488 (1:1000; Fisher Scientific, S32354) at room temperature for one hour ...
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bioRxiv - Immunology 2020Quote: ... at 37 °C in Alexa Fluor 488 phalloidin (Life Technologies, 1:40 v/v in blocking buffer) and Alexa Fluor 594 labelled anti-human DAP10/HCST (R&D Systems ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies were diluted in blocking buffer (goat anti-rabbit Alexa Fluor 555, 1:1000 (Invitrogen 32732); goat anti-mouse Alexa Fluor 555 ...
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bioRxiv - Neuroscience 2019Quote: ... We replaced the blocking solution with primary antibody solution (1:500 anti-GFP-rabbit IgG; Life Technologies) for 12 hours at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... and incubated for 1 hr in blocking buffer (10% Normal Goat Serum; Thermo Fisher Scientific; Waltham, MA) containing 0.3% Triton X-100 ...
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bioRxiv - Pathology 2021Quote: ... Membranes were blocked for 1 hour at RT using Pierce™ Clear Milk Blocking Buffer (ThermoFisher Scientific). Blots were incubated overnight at 4°C with mouse anti-FLAG tag mAB (Cell Signaling Technology ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained as follows: 1-blocking with 2 μg/mL FcBlock (anti mouse CD16/CD32, Invitrogen) for 1 h at RT in microscopy buffer (5% w/V BSA (Sigma) ...
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bioRxiv - Developmental Biology 2020Quote: ... we incubated samples in BODIPY® FL Phallacidin (1:40 dilution in blocking buffer, Thermo Fisher Scientific) at RT for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... Cross-absorbed secondary antibodies tagged with Alexa-fluorophores were diluted in blocking buffer (1:400, Invitrogen, Switzerland) and applied for 30 min at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by incubation with secondary antibodies in blocking buffer and 1 μg/ml DAPI (Thermo Fisher Scientific) in blocking solution for 50 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... 1% OVA in PBST for the second round and ELISA Blocker Blocking Buffer (Thermo Fisher Scientific, N502) for the third round ...
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bioRxiv - Developmental Biology 2023Quote: ... oocytes were incubated with the secondary antibodies (1:500 in blocking solution, Alexa Fluor, Thermo Fisher Scientific) for 1 hour in dark ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted in 7.5ml Intercept PBS blocking buffer with 0.1% Tween (SynGAP1; 1:1000dil, Invitrogen, PA1-046 ...
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bioRxiv - Immunology 2023Quote: ... was used at 1:8000 dilution in blocking solution together with 0.01 mg/ml DAPI (Thermo Fisher). The wells were washed with wash buffer and PBS ...