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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Glycerol Kinase 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... biopsies were co-cultured with 3T3-J2 fibroblasts and Rho-associated protein kinase inhibitor (Y-27632) in epithelial cell expansion medium consisting of a 3:1 ratio DMEM:F12 (Gibco, UK; 21765), 1X penicillin/streptomycin and 5% FBS (Gibco ...
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bioRxiv - Developmental Biology 2021Quote: ... and Alexa 488 conjugated anti-rabbit IgG antibody (1:500 in blocking buffer, ThermoFisher Scientific, A11034) for one hour at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked for 1 hr at room temperature in blocking buffer (PBS (Gibco 14190-144), 0.05% Tween20 (Sigma-Aldrich 274348500) ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated in PBS blocking buffer containing mouse anti-PV 1o antibody (Thermofisher, 1:500) overnight at 4oC ...
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bioRxiv - Cell Biology 2019Quote: ... and incubated in mouse α-myc primary antibody (1:250 in blocking buffer, Invitrogen R950-25) for 1 hour ...
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bioRxiv - Genetics 2020Quote: ... for 30 minutes at room temperature and blocking in 1xPBS with 1% fetal bovine serum (Gibco). Cells were incubated with primary antibody for myosin heavy chain (MF-20—1:100 ...
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bioRxiv - Biochemistry 2021Quote: ... was diluted 1:1000 in blocking solution and the second α-mouse antibody (G21234, Invitrogen AG) was diluted 1:2000 in blocking solution ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies (Table 2) were diluted 1:100 in blocking solution and secondary antibodies (Life Technologies) were diluted 1:200 in blocking solution ...
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bioRxiv - Bioengineering 2020Quote: ... for 30 minutes at RT followed by 30-minute blocking with 1% BSA/PBS (Thermofisher, 37525) at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... Section slides or embryos were incubated in blocking buffer containing specific secondary antibodies (Invitrogen,1:500) and DAPI (1:1000 ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked for 1 hour with Sea Block Blocking Solution (Thermo Fisher Scientific, Waltham, MA) and probed overnight at 4°C with 1:1000 TG2 (Abcam ...
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bioRxiv - Molecular Biology 2021Quote: ... the secondary antibody (1/400 in blocking buffer, goat anti-rabbit Alexa 594 antibody, Thermo Fisher, A-11037 or donkey anti-mouse Alexa 488 antibody ...
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bioRxiv - Immunology 2021Quote: ... diluted 1:3000 in blocking buffer or HRP-labelled goat anti-human IgM (Thermo Fisher Scientific) diluted 1:4000 at 37°C for 1 hour ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 μL of blocking buffer was added to the cell suspension (Thermo Fisher Scientific, Waltham, MA). The resulting cell suspension was incubated for 10 minutes on ice ...
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bioRxiv - Cell Biology 2022Quote: ... samples were incubated 2hrs at 4°C with secondary antibody (1:1000 in blocking solution; Invitrogen). Following extensive washing ...
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bioRxiv - Neuroscience 2023Quote: ... The blocking solution was removed and incubation with GFAP primary antibody (Invitrogen #13-0300, 1:400), diluted in PBS containing 3% goat serum with 0.2% TX-100 (PBST) ...
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bioRxiv - Bioengineering 2023Quote: ... followed by 1 h incubation in the blocking buffer (5% Fetal Bovine Serum (FBS, Gibco, USA) and 1% Bovine Serum Albumin (BSA ...
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bioRxiv - Microbiology 2023Quote: ... primary antibody and 488 (FLAG) Alexa Fluor secondary antibody (Life Technologies; 1:500 in blocking buffer), then washed and imaged as described above.
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bioRxiv - Immunology 2023Quote: ... Samples were blocked for 1 hours with BlockAid blocking solution (Thermo Fisher Scientific, catalog no. B10710)) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections then were incubated in blocking solution with chicken anti-GFP (1:1,000, Life Technologies, A10262) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... cells were first incubated for blocking with 5% BSA (bovine serum albumin, BP1600-1 Fisher Scientific) in PBT (0.3% Triton X-100 (ACROS ...
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bioRxiv - Synthetic Biology 2023Quote: Secondary antibodies were diluted in blocking buffer at 1:10,000 anti-mouse-HRP (Thermo Fisher; 31340) and 1:10,000 anti-rabbit-HRP (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated with secondary antibody in blocking buffer (Alexa conjugated antibodies, Thermo Fisher Scientific, 1:500) for 2 h at RT ...
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bioRxiv - Microbiology 2023Quote: ... and blotted with 1:1000 anti-HA-HRP conjugated antibody in blocking solution (Thermo Fisher Scientific). Chemiluminescence was detected using the Bio-Rad Chemidoc system ...
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bioRxiv - Cell Biology 2023Quote: ... cells were first incubated for blocking with 5% BSA (bovine serum albumin, BP1600-1 Fisher Scientific) in PBT (0.3% Triton X-100 (ACROS ...
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bioRxiv - Immunology 2024Quote: ... anti-DCLK1 (Rabbit anti-mouse; monoclonal: JA11-03 clone; dilution 1:200 in blocking solution; Invitrogen); anti-CD3 (Rabbit anti-mouse ...
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bioRxiv - Bioengineering 2024Quote: ... and 100 µL/well of Rabbit anti Cotton Rat IgG (1:1,000 in blocking buffer) (Invitrogen) was added and incubated for one hour at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... 0.2 % Tween) and incubated in the primary antibody (diluted in blocking solution, Cx36 1:500, ThermoFisher, 37-4600 ...
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bioRxiv - Neuroscience 2019Quote: ... Approximately 2μg peptide from each fraction was injected into an Acclaim peptide trap column (Thermo Fisher) via the Dionex RSLCnano autosampler ...
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bioRxiv - Cell Biology 2021Quote: ... 100pmol biotinylated peptides (Peptide 2.0) were bound to 8μl streptavidin M-280 Dynabeads (Thermo Fisher Scientific) for 30mins in PBS at room temperature with agitation ...
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bioRxiv - Genomics 2022Quote: ... Concentration of the stock H4K16ac peptide was analyzed by Pierce Quantitative Fluorometric Peptide Assay (Thermofisher Scientific). Spectra were collected with [15N-BD ...
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bioRxiv - Genomics 2021Quote: ... The concentration of eluted peptides was determined by Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Scientific). The peptides were dissolved in 0.1% [vol/vol] TFA and 2% [vol/vol] acetonitrile (ACN) ...
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bioRxiv - Biochemistry 2021Quote: Peptide yields for optimisation were determined using the Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... The peptide concentrations were further determined by Quantitative Fluorometric Peptide Assay (Thermo Scientific™ Pierce™). Finally ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were eluted from the corresponding beads using 3XFLAG peptide or HA peptide (Thermo Fisher Scientific). 5 μg His6-RavJ or His6-RavJC101A were added to the in vitro assay and the reaction was left in the 37°C incubator for 2 h ...
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bioRxiv - Zoology 2022Quote: ... The redissolved peptides were measured using a colorimetric peptide assay kit (Thermo Fisher Scientific, MA, USA) and transferred to a hydrophilic-coated low adsorption vial (ProteoSave vial ...
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bioRxiv - Cell Biology 2023Quote: ... and peptide concentrations were determined using the Pierce™ Quantitative Fluorescent Peptide Assay (Thermo Scientific, USA). Next ...
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bioRxiv - Cell Biology 2023Quote: ... Total peptide quantification was performed using the Pierce Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific, USA) to normalise sample peptide concentrations for LCMS analysis ...
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bioRxiv - Biochemistry 2023Quote: ... Detected peptides were searched against the protein sequences using BioPharma Finder 5.0 Peptide mapping (Thermo Fisher) and PMI Byologic (Protein Metrics Inc.).
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bioRxiv - Molecular Biology 2024Quote: ... Each fraction (∼4.5 mg peptides) was used for peptide enrichment with either M280 streptavidin beads (Invitrogen), MyOne streptavidin beads (Invitrogen) ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... Peptide – peptide interactions were detected using the SuperSignal West Pico PLUS chemiluminescent substrate (Thermo Fisher Scientific). The signals were acquired using the AI600 Imager (Amersham ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting peptide samples were desalted with a PierceTM Peptide Desalting Spin Column (Thermo Fisher Scientific) according to manufacturer’s instruction and then completely dried under speed-vacuum.
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bioRxiv - Neuroscience 2021Quote: ... Blocking solution was replaced with antibody solution consisting of blocking solution with antibodies as follows: mCherry (Invitrogen, M11217) 1:500 ...
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bioRxiv - Developmental Biology 2021Quote: ... and diluted in glycerol with a dextran-conjugated lineage tracer (ThermoFisher); RNA doses were empirically optimized ...
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bioRxiv - Cell Biology 2020Quote: ... 10% glycerol) using Slide-A-Lyzer Mini Dialysis Devices (Thermo Scientific) and concentrated using 30kDa MWCO Amicon Ultra-4 centrifugal filter units (Millipore) ...
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bioRxiv - Molecular Biology 2020Quote: ... 10% glycerol (v/v) and protease inhibitor cocktail (Halt, Thermo Fisher). Cell lysate was syringed with a 25G needle and centrifuged at 12,000×g for 5 min to pellet cell debris ...
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bioRxiv - Biochemistry 2022Quote: ... 0.1% NP40 and 10% glycerol) with protein inhibitor cocktail (Thermo Fisher) for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: ... 5% glycerol) containing fresh protease inhibitor and phosphatase inhibitor cocktail (ThermoFisher), followed by centrifugation to remove cellular debris ...
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bioRxiv - Microbiology 2020Quote: ... Tween 80 and Glycerol were purchased from Fisher Scientific (MA, USA).
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bioRxiv - Genomics 2022Quote: ... 10% glycerol) supplemented with 10 µL of benzonase (Thermo-Fisher Scientific) and one Roche cOmplete protease inhibitor tablet ...