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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Glycerol Kinase 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: 10 DAG seedlings were used for western blotting to detect the endogenous level of the phosphorylated (Phospho-p70 S6 kinase pThr389 antibody; dilution: 1:1000; catalog no.: MA5-15117, Invitrogen) and total (Ribosomal S6 kinase 1/2 antibody ...
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bioRxiv - Biochemistry 2021Quote: ... and human phosphatidylinositol 4-phosphate 5-kinase type-1 beta (hPIP5KB; Uniprot #O14986) were derived from codon optimized genes synthesized by GeneArt (Invitrogen). The gene encoding yeast Mss4 (Uniprot #P38994 ...
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bioRxiv - Biochemistry 2022Quote: ... which was hydrolyzed by adding 1.5 μL of 10 units/μL T4 PNK (polynucleotide kinase) and 1 μL RNase-Out solution (40 units/μL, ThermoFisher) to the cleavage reaction above ...
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bioRxiv - Cell Biology 2023Quote: ... Fms-like tyrosine kinase 3 ligand (Flt3- ligand; 100 ng/mL) and 1% penicillin-streptomycin (GIBCO, Grand Island, NY, USA), in the presence or absence of G-CSF at a dose of 100 ng/mL ...
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bioRxiv - Cell Biology 2019Quote: ... The peptide concentration was determined using a Pierce quantitative colorimetric peptide assay (Thermo Fisher Scientific). Approximately 150 μg of peptides for each sample was labeled with 200 μg of TMT-6plex reagent (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Peptide concentrations were then determined using the Pierce™ Quantitative Colorimetric Peptide Assay (Thermo Scientific) and dried by vacuum concentration ...
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bioRxiv - Cell Biology 2021Quote: ... The peptides in all six sample were quantified using Pierce Fluorescent Peptide assay (Thermo Scientific).
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bioRxiv - Plant Biology 2022Quote: ... Peptide concentration was determined using a PierceTM Quantitative Colorimetric Peptide Assay kit (23275, Thermo Scientific). 100µg peptide aliquots were labelled using TMT10plexTM (90110 ...
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bioRxiv - Microbiology 2020Quote: ... Peptide quantitation was performed using BCA (Pierce Quantitative Colorimetric Peptide Assay) or Qubit (Thermo Scientific) system.
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bioRxiv - Cell Biology 2022Quote: ... the quantification of peptide was performed using Pierce Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific). Then ...
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bioRxiv - Biochemistry 2021Quote: ... peptide totals were measured using Quantitative Colorimetric Peptide Assay (Thermo Scientific Pierce, cat. no.: 23275).
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bioRxiv - Neuroscience 2019Quote: ... Peptide concentrations were determined using the Pierce Quantitative Colorimetric Peptide Assay (Thermo Scientific; Cat# 23275). Approximately 300 µg of peptide was recovered from each digest ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were ordered at Peptides and Elephants (Hennigsdorf, Germany) or from ThermoFisher (Waltham, MA, USA) as TFA salts and dissolved as 50 mM stock solution in DMSO or in ddH2O for GAP10 and GAP16 experiments in Figs 1 ...
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bioRxiv - Plant Biology 2020Quote: ... Peptides were then quantified using Pierce Quantitative Fluorometric Peptide Assay (Thermo Scientific, catalogue number 23290) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Peptides were fractionated using a Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific) according to the manufacturer’s instructions with slight modifications ...
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bioRxiv - Microbiology 2020Quote: The concentrations of the peptide soups were measured with Quantitative Colorimetric Peptide Assay (Thermo Scientific). TMT10plex amino reactive reagents (0.8 mg per vial ...
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bioRxiv - Cell Biology 2021Quote: ... The resulting peptides were quantified using a quantitative colorimetric peptide assay kit (Pierce/Thermo Scientific) and an equal amount of each labelled with Tandem Mass Tag (TMT ...
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bioRxiv - Neuroscience 2023Quote: ... Peptide concentration of the supernatant was quantified using a quantitative colorimetric peptide assay (Thermo Fisher). For TMT labelling we used the TMT-11plex kit (Thermo Fisher)53 ...
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bioRxiv - Neuroscience 2023Quote: ... Peptide concentrations were determined using the Pierce Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific 23290) following the descriptions of the manufacturer ...
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bioRxiv - Plant Biology 2023Quote: ... Desalting of peptides was done using Peptide Desalting Spin Columns (Pierce, Thermo Scientific, United States) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The peptide concentrations were determined using the Pierce quantitative colorimetric peptide assay (Thermo Fisher Scientific). Approximately 25 µg of peptides for each sample was labeled with 0.2 mg of TMT-10plex reagents (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Peptide concentrations were determined using Pierce Quantitative Colorimetric Peptide Assay (cat# 23275, Thermo Fisher Scientific), and samples were frozen at −80°C until LC-MS data acquisition.
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bioRxiv - Biochemistry 2023Quote: ... The peptide concentration was measured using Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific) by following the manual.
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bioRxiv - Cell Biology 2024Quote: ... labeled peptides were fractionated using Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific). A total of 6 fractions were collected ...
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bioRxiv - Systems Biology 2024Quote: ... The labeled peptides were separated by High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific) to obtain 10 different fractions ...
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bioRxiv - Biophysics 2019Quote: ... The unbound Rep-X was washed off simultaneously and the unwinding reaction was initiated by adding the unwinding buffer (10 mM Tris-HCl, pH 8, 100 mM KCl, 2.5 mM MgCl2, 1 mM ATP [ThermoFisher Scientific], 10 % glycerol, 1 % BSA). For characterization of the maiden folded GQ conformation ...
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bioRxiv - Biophysics 2019Quote: ... The unbound Rep-X was washed off simultaneously and the unwinding reaction was initiated by adding the unwinding buffer (50 mM Tris-HCl, pH 7.5, 100 mM KCl, 5 mM MgCl2, 1 mM ATP [ThermoFisher Scientific], 10 % glycerol, 1 % BSA). The unwinding reaction was quenched after a minute to capture the conformation of maiden folded RNA aptamers ...
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bioRxiv - Genetics 2019Quote: ... was applied at a final concentration of 1:300 in blocking buffer for 1 hr and Hoechst 33342 (ThermoFisher #H3570) applied for 1-5 min at a concentration of 1:5000 ...
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bioRxiv - Molecular Biology 2019Quote: ... Primary antibodies were diluted in blocking buffer and incubated at room temperature for 1 hour (Sigma Aldrich M2 Flag at 1:1,000; Invitrogen 22C5D8 Pgk1 at 1:6,000 ...
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bioRxiv - Immunology 2020Quote: ... and blocked with 200 µL blocking buffer (TBST-MS: 1x TBST + 1% w/v milk, 1% v/v goat serum (Gibco) for 1 h at RT ...
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bioRxiv - Cell Biology 2022Quote: ... sections were washed and incubated for 1 h with Alexa Fluor conjugated secondary antibodies (1:1000 in blocking solution; Invitrogen). Following extensive washing ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were incubated with fluorophore-conjugated secondary antibodies in blocking solution for 1 hr (1:200, Alexa 488 or 555, Invitrogen) then mounted with DAPI-containing Fluoromount medium (SouthernBiotech) ...
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bioRxiv - Cell Biology 2023Quote: ... After blocking with 1% normal donkey serum (NDS, Biozol, END9010) and 1% bovine serum albumin (BSA, Thermo Fisher Scientific, 23209) in PBS ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were successively incubated with primary antibodies diluted in blocking buffer (mouse anti-G3BP1, 1:4000, BD Biosciences, rabbit anti-GFP, 1:1000, ThermoFisher, mouse anti-PKR ...
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bioRxiv - Developmental Biology 2021Quote: ... then incubated in 1 mL the secondary staining solution (goat anti-rabbit-AlexaFluor 488 [Thermofisher] at 1:1000, Hoechst 33342 at 1:1000 [Thermofisher] and Phalloidin-AlexaFluor555 at 1:200 [Thermofisher] in blocking buffer). The tissue was washed extensively and then mounted on glass slides using VectaShield (Vector Labs ...
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bioRxiv - Microbiology 2020Quote: ... These peptides were captured in a 1-mL 96-well plate (Thermo Scientific, #AB-1127) and the volume was dried down in a Centrivap speedvac (Model 7810016) ...
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bioRxiv - Plant Biology 2022Quote: ... Dissolved peptides (1 μg) were injected using an Easy-nLC 1200 system (LC140; Thermo Scientific) and separated on a 50 cm Easy-Spray PepMap C18 Column (ES803A ...
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bioRxiv - Cancer Biology 2020Quote: ... The peptides were then labelled with TMT reagent (1:4; peptide:TMT label)(Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2021Quote: Tryptic peptides (1 μg) were analyzed with an LTQ-Orbitrap Velos ETD (Thermo Fisher Scientific) coupled with Easy NanoLC II (Thermo Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... Approximately 1 µg of peptides were separated by an online reversed-phase HPLC (Thermo Scientific Dionex Ultimate 3000 RSLC nano LC system ...
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bioRxiv - Biochemistry 2023Quote: ... peptides were resolved with an analytical C18 column (Hypersil GOLD 1×50 mm, Thermo Scientific) and quickly eluted into a Bruker maXis-II ETD UHR ESI-QqTOF spectrometer at a flow rate of 40 μL/min for mass determination.
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bioRxiv - Developmental Biology 2022Quote: ... Gene-Tools) in injection solution: 10% glycerol and 1 mM Texas Red Dextran or 0.83 mM FITC Dextran (Molecular Probes). After incubation for 1 day at 16°C ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... for 15 min at RT followed by mounting with MM or Hoechst 33342 (1:1000 in glycerol/PBS) (Invitrogen; LSH3570) in MM.
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bioRxiv - Cell Biology 2022Quote: ... 100 mM NaCl, 0.5% sodium deoxycholate, 10% glycerol, 1% Triton X-100 with Pierce protease and phosphatase inhibitor [Thermo Scientific]). Cell culture supernatant samples were also collected ...
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bioRxiv - Cell Biology 2022Quote: ... 10 % glycerol and 50 mM Tris-HCl (pH 8.0)) supplemented with 1× EDTA-free Halt protease inhibitor cocktail (ThermoFisher Scientific), 1 mM EGTA (pH 7,7) ...
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bioRxiv - Cell Biology 2023Quote: ... The protein samples were denatured by adding 3× SDS sample buffer (containing 188 mM Tris-HCl, pH 6.8; 3% SDS; 30% glycerol; 0.01% bromophenol blue; and 1× Bond-Breaker TCEP solution from Thermo Fisher Scientific) and heating at 95°C for 10 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... the buffer was exchanged to HBS buffer (1×HBS pH 7.2, 5% (v/v) glycerol) by gel filtration (Zeba Spin Desalting column, 2 mL, Thermo Fisher). For immunoblotting ...
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bioRxiv - Neuroscience 2023Quote: ... microslide was rinsed with running tap water again for 1 min, coverslipped (Premium Cover Glasses, EF15972L, Daigger Scientific) with UltraPureTM glycerol (15514-011, Invitrogen), and visualized with a Brightfield microscope (Leitz Laborlux S Wild GMBH) ...
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bioRxiv - Biophysics 2023Quote: ... 300 mL NaCl, 5 mM imidazole, 10% glycerol, 0.2 mM 2-mercaptoethanol, 1 mM PMSF, and Pierce Protease Inhibitor Tablets [Thermo Fisher]). Cells were sonicated for 5 min (30% amplitude ...
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bioRxiv - Biochemistry 2021Quote: 15 pmol of RNA/DNA were radioactively labeled for 1 h at 37 °C using 5 U T4 Polynucleotide Kinase (Thermo Fisher) and 10 µCi γ-32P ATP ...