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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for Glycerol Kinase 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... permeabilized in 0.25% Triton X-100 and blocked in Sea Block Blocking Buffer (1 h; ThermoFisher Scientific, USA). Overnight incubation with primary antibodies was followed by the incubation (1 h ...
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bioRxiv - Cell Biology 2019Quote: ... and mouse anti-GM130 (1:500) diluted in blocking solution with 0.1% saponin and 2.5% goat serum (Gibco) for 1 hour ...
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bioRxiv - Developmental Biology 2019Quote: ... the cells were incubated with secondary antibodies (1: 500 dilution in blocking buffer, Alexa Fluor 488, Life Technologies) at room temperature for 1 hour in the dark ...
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bioRxiv - Physiology 2021Quote: ... and incubated overnight with the primary antibodies diluted in blocking buffer: anti-TSG101 (1:250, PA531260, Thermo scientific) 1:250 ...
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bioRxiv - Biochemistry 2021Quote: ... and mouse anti-UBE2J1 (1:250) diluted in blocking solution with 0.1% saponin and 5% goat serum (Gibco) for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 2 hours of incubation in blocking solution containing anti-chicken Alexafluor488 (1:400; Life Technologies A11039) and anti-goat Alexafluor594 (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... which was detected with Alexa488 conjugated goat anti-mouse (1:250 in blocking solution; Invitrogen cat No. A11001). A GABA-IR detection system (secondary antibody and streptavidin-Cy3 ...
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bioRxiv - Genomics 2022Quote: PVDF membranes containing transferred proteins were incubated in blocking buffer (1 x TBS-T (Thermo Fisher Scientific, 28360), 5% Blotting-Grade Blocker (BioRad ...
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bioRxiv - Neuroscience 2022Quote: ... followed by incubation for 1 h in blocking solution containing 5% goat serum (16210–064, Thermo Fisher Scientific), 3% bovine serum albumin (BAC62 ...
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bioRxiv - Molecular Biology 2022Quote: ... Chambers are blocked in blocking buffer (DPBS, 0.5% BSA, 0.05% Triton X-100, 1:100 SUPERase·InTM (Invitrogen, AM2694)) for one hour at room temperature and YTHDF1 antibody (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... diluted in blocking buffer: Alexa Fluor 488 donkey anti-mouse IgG (H+L) (1:500, Invitrogen A-21202), Alexa Fluor 488 donkey anti-goat IgG (H+L ...
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bioRxiv - Microbiology 2023Quote: Cells were washed with FACS buffer (0.2% BSA, 1 mM in PBS) before blocking with Fc block (Invitrogen) and incubation with eF780 live/dead viability stain (eBioscience ...
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bioRxiv - Systems Biology 2021Quote: ... total peptide concentration in each sample was determined using the Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific) to ensure that the same total peptide amount across samples 1-17 and 28-35 (excluding samples 18-27 ...
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bioRxiv - Plant Biology 2021Quote: ... The peptide concentration of the reconstituted solution was determined by using Pierce quantitative colorimetric peptide assay (Thermo Scientific, USA).
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bioRxiv - Biophysics 2022Quote: ... and the modified peptides were used for immunoaffinity purification according to manufacturer’s instructions (SulfoLink Immobilization Kit for Peptides, ThermoFisher). 72 µL of PAS beads was incubated overnight with 100 µg purified SMC1 antibody and 100 µg purified SMC3 antibody ...
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bioRxiv - Systems Biology 2023Quote: ... peptides were quantified by BCA assay and 150 μg of peptide per sample were labeled with TMTPro reagents (ThermoFisher) for 2 hrs at room temperature ...
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bioRxiv - Microbiology 2022Quote: The maleimide labeling of the HR2 peptides was performed by mixing 100 µM peptide and 2.5 mM fluorescein-5-maleimide (ThermoFisher) in degassed buffer (50 mM sodium phosphate ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants containing the obtained peptides were purified using Pierce™ Peptide Desalting Spin Columns (Thermo Scientific, Bartlesville, OK, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Phosphorylated peptides were enriched from 1mg of total peptides using High-Select Fe-NTA Phosphopeptide Enrichment Kit (Thermo Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... and peptide quantitation was performed using the Pierce™ Quantitative Peptide Assays & Standards (Thermo Fisher Scientific, Waltham, United States).
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bioRxiv - Evolutionary Biology 2023Quote: ... and peptides were purified using HiPPR detergent removal kit and Pierce peptide desalting spin columns (both Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were resuspended in running solvent and quantitated using a Pierce Colorimetric Peptide Quantitation Assay (Thermo Fisher Scientific, 23275).
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bioRxiv - Biochemistry 2024Quote: ... Peptides were resuspended in running solvent and quantitated using a Pierce Colorimetric Peptide Quantitation Assay (Thermo Fisher Scientific, 23275). Peptides were separated and analyzed as described above ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were probed with primary antibodies in SuperBlock T20 (TBS) Blocking Buffer and fluorophore-conjugated secondary antibodies in SuperBlock (TBS) Blocking Buffer (Thermo Scientific), with 3 washes in SuperBlock T20 (TBS ...
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bioRxiv - Neuroscience 2020Quote: ... Tissues were then washed 3 times in PBS and incubated with appropriate fluorochrome secondary antibody diluted in blocking solution for 1-2h (AlexaFluor® 488, AlexaFluor® 568, 1:400, Life Technologies). Finally ...
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bioRxiv - Developmental Biology 2019Quote: ... incubated for 30 minutes with the appropriate secondary antibodies (1:400 dilution in blocking buffer) and/or Alexa FluorTM-647 conjugated phalloidin (1:400, Invitrogen, A22287, lot 1884190), and washed twice more with PBS ...
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bioRxiv - Immunology 2022Quote: ... Virus infected cells were detected by staining with a primary rabbit anti-VACV antibody (1:1000 in blocking buffer for 1 hour, Thermo Fisher PA1-7258) and a secondary horseradish peroxidase-conjugated goat anti-rabbit antibody (1:1000 in blocking buffer for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated for 1 h with 488 (FLAG) and 594 (LCMV NP) Alexa Fluor antibodies (Life Technologies; 1:500 in blocking buffer), then washed four times with PBS.
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bioRxiv - Cell Biology 2023Quote: ... and anti-N-WASP (Cell Signalling #4848S; 1:100) followed by Alexa Fluor 568 conjugated secondary antibodies (Invitrogen; 1:1000 in blocking buffer). Actin tails were labeled with Alexa Fluor 488 phalloidin (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated with a cocktail of secondary antibodies (1:500, Alexa Fluor 488, 546, 647) in blocking solution containing Hoechst 33342 (1:10,000, Life Technologies, Cat. No. H3570) overnight at room temperature followed by three final washes with PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mL min-1) and identity of peptides confirmed by high-resolution mass (Q Exactive Orbitrap LC-MS/MS (Thermo Fisher Scientific).
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bioRxiv - Immunology 2024Quote: ... A polyclonal antibody against the ROTR-1 protein was raised using the peptide LDRSPPSDDGTQKV (ROTR-1 amino acids 288 to 301) in rabbit (Thermo Fisher Scientific). Anti-ROTR-1 sera was used at a dilution of 1:1000 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were 5′-phosphorylated with polynucleotide kinase (Thermo Fisher Scientific, Waltham, MA, USA), and circularised using T4 DNA ligase (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2019Quote: DNA samples were treated with 100 U of T4 polynucleotide kinase (PNK, Thermo Fisher) in the presence of 1 mM dATP (Roche ...
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bioRxiv - Biochemistry 2021Quote: ... anti-Skp2 and anti-Aurora B Kinase antibodies were purchased from Invitrogen (Carlsbad, CA). Antibodies against Cdt1 ...
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bioRxiv - Microbiology 2019Quote: ... Blunt ends of the purified linear fragment were phosphorylated with T4 Polynucleotide Kinase (ThermoFisher) followed by overnight treatment with T4 DNA Ligase (NEB ...
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bioRxiv - Genomics 2022Quote: ... To phosphorylate the pooled probes a T4 polynucleotide kinase kit (ThermoFisher, Waltham, MA, USA) was used by following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... were labeled with [γ-32-P]-ATP using T4 polynucleotide kinase (Thermo Fisher Scientific) and hybridized to the membrane in hybridization buffer (4× Denhardts solution ...
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bioRxiv - Biochemistry 2023Quote: In vitro screening for kinase inhibition by compound C1 was performed by Thermo Fisher Scientific (Madison ...
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bioRxiv - Cancer Biology 2023Quote: ... Double-stranded oligonucleotides were then concatemerized with 100 U T4 polynucleotide kinase (Thermo Scientific) and 20 Weiss U T4 DNA ligase (Thermo Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... rRNA-depleted ribosome footprints were treated with T4 polynucleotide kinase (PNK; ThermoFisher, cat#EK0031). The end-repaired ribosome footprints were purified using the Monarch RNA cleanup kit using the modified protocol for small RNA ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: ... Glycerol stocks of desired clones were midiprepped using anion-exchange columns (Invitrogen #K210015) following growth in 50ml LB + carbenicillin medium for 10-12 hours ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were then cleared in glycerol (525342C, Thermo Fisher Scientific, Waltham, MA, USA). Ethidium bromide-stained and TRAP-stained samples were imaged on a dissecting microscope (Leica MZFLIII ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Mounting was made in 80%glycerol/PBS with Hoechst-33342 1μM (Invitrogen-62249). A confocal microscopy LSM880 (Zeiss ...
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bioRxiv - Developmental Biology 2020Quote: ... and 10 mM β-glycerol phosphate (AC410991000, Thermo Fisher Scientific, Waltham, MA, USA) for 5 days ...
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bioRxiv - Microbiology 2020Quote: ... all bacterial isolates were seeded from glycerol stocks in BHI (Oxoid, Thermo Scientific) media and incubated for 24 h before placing on either BHI agar or M9 minimal agar media supplemented with either 100 mM sucrose (Acros Organics ...
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bioRxiv - Microbiology 2021Quote: ... with 60 mL deionized water and 100 mL molecular-grade glycerol (Acros Organics). This mixture was filter-sterilized using a 0.2 micrometer filter ...
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bioRxiv - Biophysics 2023Quote: ... 5% glycerol) supplemented with EDTA-free Halt protease inhibitor cocktail (Thermo Fisher Scientific). Cells were lysed for 15 min followed by centrifugation at 14,000 RPM for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... the slide was coverslipped with 85% glycerol supplemented with RiboLock RNase inhibitors (Thermofisher, Cat# EO0382 ...
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bioRxiv - Bioengineering 2023Quote: ... To this mixture we added glycerol 2-phosphate (3 M, Thermo Fisher Scientific) and (GT)15-SWCNTs (3.75 mg/L ...