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Citations for Thermo Fisher :
551 - 600 of 6145 citations for GDF 11 BMP 11 Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Briefly, cortices were dissociated, and diluted in 10% Fetal Bovine Serum (OmegaSci, FB-11)/1% penicillin-streptomycin in DMEM (Gibco, 11995-065) and plated at a density of one brain per uncoated T75 flask ...
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bioRxiv - Biochemistry 2023Quote: ... DNA quantity was checked with fluorometry on the DS-11 FX instrument (DeNovix) using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific) and sizes were examined on a 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... only the early fractions that contained the 40S peak to avoid eIF3-bound 40S subunits) were pooled and pelleted in a 11 x 34 mm thin-wall polycarbonate tube (Thermo Fisher # 45315) at 55,000 rpm for 18 hrs at 4°C in a Sorvall S55-S rotor using a Sorvall Discovery M120 SE Micro-Ultracentrifuge ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-APP (22C11, 1:1000 dilution), Anti-β Amyloid (D-11 Santacruz, Cat. Sc-374527, 1:1000) and anti-β-tubulin (Invitrogen Cat.32–2600 or Proteintech Europe ...
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bioRxiv - Neuroscience 2023Quote: ... in electron-event representation mode with a flux of 11 electrons/pixel/s and a Selectris-X energy filter (Thermo Fisher Scientific) with a slit width of 10 eV to remove inelastically scattered electrons ...
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bioRxiv - Neuroscience 2023Quote: ... HPCs were converted into homeostatic microglia with a differentiation medium composed of DMEM/F12 basal media (Gibco, Catalog No. 11-320-033), 2X 100X Insulin-Transferrin-Selenium (ITS-G)(Gibco ...
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bioRxiv - Molecular Biology 2024Quote: ... An expression backbone originally containing wild-type Cas7-11 (pDF0506) was digested with Fermentas FD0596 NotI and Fermentas FD1464 AgeI (Thermo Fisher Scientific) with 10x FastDigest Buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... peptides were resuspended in 10 µL of water and labeled for 1 h at room temperature with 4 µL (∼11 µg/µL in acetonitrile) of TMT10plex or TMT18plex (Thermo Fisher Scientific). The reaction was quenched with 4 µL of 5% hydroxylamine ...
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bioRxiv - Pathology 2024Quote: Sex-matched 9 to 11-week-old wild-type TGFβ1-Tg+ mice received 0.5 mg/mL doxycycline hyclate (Thermo Scientific Cat No. J60579.22) in their drinking water for 21 days as previously described [35].
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bioRxiv - Cell Biology 2024Quote: ... Quantitative RT-PCR was conducted with gene-specific primers (refer to Supplementary Table 11) using either THUNDERBIRD Next SYBR qPCR Mix (TOYOBO) or TaqMan assays (Thermo Fisher Scientific) with TaqMan Universal Master Mix II ...
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bioRxiv - Microbiology 2024Quote: ... The concentration and quality of DNA were evaluated by NanoDrop DS-11+ Spectrophotometer (DeNovix, Wilmington, U.S.) and Qubit 2.0 Fluorometer (Thermo Fisher Scientific, Massachusetts, U.S.). From each sampling site ...
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bioRxiv - Cell Biology 2024Quote: ... pcDNA3.1-LZTR1-Myc-6xHis plasmid was a gift from Jens Kroll (Heidelberg University and German Cancer Research Center (DKFZ-ZMBH Alliance)).56 The LZTR1L580Pvariant was introduced into the plasmid by site-directed mutagenesis as previously described.11 Cells were transfected using ExpiFectamine 293 Reagent (Thermo Fisher Scientific) and cultured at a density of 3-5×106 cells/ml in a 37°C incubator with ≥80% relative humidity and 8% CO2 on an orbital shaker at 125×g for 3-4 days ...
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bioRxiv - Neuroscience 2020Quote: Total RNA from HEK293 cells and human brain prefrontal cortex were isolated with the TRIzol reagent (Ambion) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2020Quote: Human Embryonic Kidney 293 cells (HEK293, ATCC CRL-1573) were grown under standard conditions in DMEM (Gibco) supplemented with 10% FBS (BioConcept) ...
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bioRxiv - Neuroscience 2023Quote: The human embryonic kidney cells (HEK293 and HEK293T) were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 15% Fetal Bovine Serum (FBS ...
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bioRxiv - Biochemistry 2022Quote: HEK293 and commercially available Flp-In T-REx HEK293 cells (Invitrogen; R78007) were cultured in DMEM (GIBCO ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells (ThermoFisher Scientific) were cultured in DMEM/F-12 ...
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bioRxiv - Bioengineering 2022Quote: ... FreeStyle HEK293 cells (ThermoFisher) were used for recombinant S protein production ...
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bioRxiv - Molecular Biology 2019Quote: ... HEK293 FT cells (ThermoFisher) were co-transfected with plasmids pCMV-dR8.2 dvpr (gag-pol ...
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bioRxiv - Synthetic Biology 2019Quote: HEK293 cells (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293-FT (Invitrogen # R70007) cells were grown in Dulbecco’s Modified Eagle Medium (ThermoFisher #11965118 ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 (Thermo Fisher Scientific) were cultured in DMEM (Dulbecco’s Modified Eagle Medium ...
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bioRxiv - Microbiology 2022Quote: ... α-mannose binding lectin C (primary: mAb 14D12, Abcam catalog no. ab106046; secondary: Mouse anti-Rat IgG2a, FITC, Thermofisher catalog no. 11-4817-82), α- IgM (mAb II/41 ...
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bioRxiv - Molecular Biology 2021Quote: ... for 60 min followed by another digestion using 1000 U/ml collagenase type 2 and 11 U/ml dispase (Gibco, cat# 17105-041) for 30 min ...
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bioRxiv - Microbiology 2020Quote: 50µg of peptides from each sample were labeled with isobaric tandem-mass-tag (TMT) 11 plex reagents (Thermo Fisher Scientific, San Jose, CA) in 20 mM pH 8.5 HEPES with 30% acetonitrile (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... After washing the sample with washing buffer (PBS, 1% Bovine Serum Albumin (BSA), cells were centrifuged (10 min, 150g, RT) and stained for microglia marker CD11b (Invitrogen, 11-0112-81) (1:100) ...
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bioRxiv - Microbiology 2022Quote: ... the cells were stained overnight at 4 °C with E1 or nsP1 primary rabbit antibodies (both antibodies previously validated in our laboratory) [11] followed by Alexa Fluor 568 goat anti-rabbit secondary antibody (Invitrogen, Carlsbad, CA, USA). Finally ...
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bioRxiv - Microbiology 2022Quote: ... α-mannose binding lectin A (primary: mAb 8G6, Hycult catalog no. HM1035; secondary: Mouse anti-Rat IgG2a, FITC, Thermofisher catalog no. 11-4817-82), α-mannose binding lectin C (primary ...
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bioRxiv - Neuroscience 2022Quote: ... HPCs in suspension were collected and spun down at 300 xG for 6 minutes and resuspended into microglia basal media (MBM, 2mL/well) containing: DMEM/F12 no phenol (Gibco # 11-039-021), 2% Insulin Transferin Selenite (Gibco # 41400045) ...
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bioRxiv - Cancer Biology 2020Quote: ... Concentration was determined with the spectrophotometer/fluorometer DeNovix DS-11 Fx and with Qubit™ 1X dsDNA HS Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Physiology 2021Quote: ... Treated RNA (11 μL per sample) was used to produce cDNA by means of the SuperScript III Reverse Transcriptase (Life Technologies, Carlsbad, US) and a 1:1 mix of Random Hexamers and 10 μM Oligo(dT ...
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bioRxiv - Immunology 2021Quote: ... at 1/100-200 dilution at RT for 1-2h in dark humidified incubation chamber or isotype control antibodies including Rabbit IgG-FITC (Invitrogen, 11-4614-80), Armenian Hamter IgG-PE (Invitrogen ...
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bioRxiv - Physiology 2021Quote: ... gestation day between E9-11) were isolated and the tissue was fragmented into pieces followed by incubation in Trypsin-EDTA (0.05 %) (Thermo Fisher Scientific, Waltham, USA) for 15 min at 37°C ...
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bioRxiv - Genetics 2020Quote: ... The cells were incubated at 37°C for up to 20 min (day 11) or up to 35 min (days 30 and 52) before adding DMEM:F12 (Thermo Fisher Scientific, 10565-018) supplemented with 10 μM Rock inhibitor and 33 μg/mL DNase I (Worthington ...
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bioRxiv - Immunology 2022Quote: ... and cells were flushed out from the cavity into RPMI medium-1640 (Cat. 11-875-093; Gibco, Thermo Fisher, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (Cat ...
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bioRxiv - Immunology 2022Quote: ... and cells were flushed out from the cavity into RPMI medium-1640 (Cat. 11-875-093; Gibco, Thermo Fisher, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (Cat ...
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bioRxiv - Cell Biology 2022Quote: ... The growth medium was changed every 2 – 3 days and consisted of RPMI-1640 (for the RIN14B cells) (Fisher Scientific, 11-875-093) or otherwise DMEM/F12 (Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... and cells were fixed by using a fixing solution prepared by adding 4 ml of glutaraldehyde and 11 ml of formaldehyde to 500 ml of Dulbecco PBS (Thermo Fisher Scientific #14190144). After 10 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... and the plasmid pcDNA3-hLOXL2 was used to amplify the SV40Pr-Neo-term sequence (primers 11-12) using the Phusion High-Fidelity DNA Polymerase (Thermo Fisher Scientific; #F530). These two PCR products were cloned into the digested repair vector GW209_pCRIS-PITChv2-C-dTAG-Puro (BRD4 ...
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bioRxiv - Biochemistry 2023Quote: ... 500 µL of perfusate was dried in 0.5 mL V-vials and reconstituted in 50 μL of 20 mg/mL hydroxylamine hydrochloride (CAS# 5470-11-1; Acros organics, New Jersey) in pyridine (CAS#25104 ...
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bioRxiv - Immunology 2023Quote: Another set of harvested lungs and spleen immune cells were stained with recommended dilutions of antibodies conjugated to fluorophores: CD3-FITC (Invitrogen, #11-0032-82) CD4-SB600 (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... Human endothelial cells in muscles were stained with biotinylated Ulex Europaeus Agglutinin I Lectin (UEA I, B-1065-2, Vectorlab, 1:100 dilution) and FITC-streptavidin (11-4317-87, Invitrogen, 1:250 dilution). Nuclei was stained with DAPI ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The reaction was stopped using Stop Reagent (1:11, in 1XPBS, Alexa Fluor 594 Tyramide SuperBoost Kit, Invitrogen, Thermo Fisher Scientific, Ghent, Belgium) for 2’ at RT ...
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bioRxiv - Immunology 2023Quote: ... Neutrophils were isolated using the MACSxpress Whole Blood Neutrophil Isolation Kit (Miltenyi, 130-104-434) and resuspended in RPMI (Gibco, 11-875-119) containing 250nM Cytotox Green (Sartorious ...
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bioRxiv - Cell Biology 2023Quote: Human embryonic kidney (HEK293) cells were obtained from ATCC and cultured in Dulbecco’s Modified Eagle’s medium (DMEM, Gibco) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2023Quote: ... Plasmids were then transfected into human embryonic kidney cells (HEK293-E, National Research Council, Canada) using 293Fectin (Thermofisher). Six days post-transfection ...
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bioRxiv - Cell Biology 2021Quote: ... Vero cells (ATCC, Manassas, VA, USA, Cat #CCL-81) were plated at 4 × 106 cells/T75 flask in MEM (ThermoFisher, Cat #11-095-080) containing 2% FBS (Peak Serum ...
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bioRxiv - Zoology 2021Quote: ... 150 μg of each lysate was digested with trypsin and labelled with Tandem Mass Tag (TMT) 11-Plex reagent kit according to the manufacturer’s instructions (Thermo Fisher Scientific, Waltham, MA, USA). In order to normalize across multiple TMT kits ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 × 10^6 cells were washed with PBS supplemented with 0.5% BSA and 0.1% sodium azide and stained with FITC-conjugated anti- CD5 (eBioscience, cat. 11-0051-85) and with PE-Cy7-conjugated anti-CD19 (Invitrogen , cat. 25-0193-82) antibodies ...