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Citations for Thermo Fisher :
351 - 400 of 6145 citations for GDF 11 BMP 11 Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... and then stained for markers at 5 μg/ml for IL-17A-FITC (Thermofisher, 11-7177-81) and IFNg-PE (Thermofisher ...
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bioRxiv - Physiology 2023Quote: ... synovial tissue was minced in Roswell Park Memorial Institute (RMPI) 1640 media (Fisher Scientific, 11-875-093) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2023Quote: ... SYBR Green I® nucleic acid gel stain and SYTO® 11 were purchased from Thermo Fisher Scientific® (Waltham ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and HepG2 (ATCC HB-8065) cells were cultured in complete DMEM/F-12 (Gibco 11- 039-02), RPTEC media (ATCC PCS-400-030 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... by following the official protocol and eluted with 11 μL of nuclease-free water (Thermo Scientific, R0582). We then pooled 2·7 μL of each barcoded amplicon ...
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bioRxiv - Microbiology 2023Quote: ... The ER-tracker solution was replaced by a 1/20 000 solution of SYTO-11 (Molecular Probes) DNA dye for 10 minutes at 26°C diluted in the appropriate Aag2 cell culture medium ...
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bioRxiv - Neuroscience 2023Quote: ... 10 individual samples and one composite sample were labeled using the TMT 11-plex kit (ThermoFisher 90406). Labeling was performed as previously described (Higginbotham et al. ...
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bioRxiv - Microbiology 2024Quote: ... and 50 µL spots were pipetted onto BHIS agar plates supplemented with 11 mM glucose (ThermoFisher Scientific) and 4 µg/mL Amp ...
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bioRxiv - Molecular Biology 2024Quote: ... and 40 ng of Cas7-11 expressing plasmid were cotransfected using Lipofectamine 3000 (Thermo Fisher Scientific, L3000001) according to manufacturer standards.
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bioRxiv - Molecular Biology 2024Quote: ... Glucose starvation was carried out in glucose-free medium: Glucose-free DMEM (Thermo Fisher, 11-966-025) supplemented with 10% FBS (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... Pelleted neurons were resuspended in neuronal growth media (DMEM-F12 with HEPES (Fisher Scientific 11-330-032), penicillin-streptomycin (ThermoFischer Scientific 15140-122) ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse MHC-I FITC conjugated H2-Dd/H-2Kd clone 34-1-2S (Invitrogen #11-5998-82), mouse MHC-I PE conjugated H-2Kb clone AF6-88.5.5.3 (Invitrogen #12-5958-82).
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: ... Eluted peptides were vacuum dried and tandem mass tags labeling was performed (11-plex TMT, Thermo Scientific) by following manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with BMP-4 (Thermo Scientific), FGF-β ...
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bioRxiv - Neuroscience 2019Quote: Human embryonic kidney (HEK293) cells were maintained in Dulbecco’s modified eagle medium (Gibco) containing 10% fetal bovine serum (HyClone) ...
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bioRxiv - Neuroscience 2020Quote: Human embryonic kidney cells (HEK293) were cultured in Dulbecco’s modified Eagle’s media (Gibco) containing 10 % v/v fetal bovine serum (heat-inactivated ...
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bioRxiv - Biochemistry 2020Quote: ... Human 28S and 18S rRNAs were extracted from HEK293 cells with TRIzol (Invitrogen). Intact rRNAs were isolated by pipetting from a native agarose gel after running the rRNA into wells in the center of the gel ...
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bioRxiv - Physiology 2022Quote: ... HEK293 cells were transfected with human TRPA1 expressing plasmid using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Human embryonic kidney (HEK293, ATCC #CRL-1573) cells were cultured in DMEM (Gibco), supplemented with 10% (vol/vol ...
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bioRxiv - Synthetic Biology 2021Quote: BMP4 concentration released into the media was quantified using the BMP-4 Human ELISA Kit from (ThermoFisher, EHBMP4). Standards and protocols were done according to the instructions of the kit ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were then labeled with TMT-11 multiplexing kit using the manufacture’s recommended method (Thermo-Fisher Scientific A34808). Channel-specific isobaric tag intensities were adjusted to 11(1:1 ...
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bioRxiv - Cell Biology 2020Quote: The hlh-11 promoter and gene were cloned using standard PCR techniques and Gateway Technology™ (Life Technologies) from N2 lysates ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were incubated in FITC-conjugated mouse anti-CD150a (PDGFRA) antibody (1:1000 dilution) (ThermoFisher 11-1401-82). Flow cytometric cell sorting and analysis was performed on a SORP FACSAria III (BD) ...
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bioRxiv - Biophysics 2019Quote: ... Nick translation was initiated by the addition of 5 µl of 250 µM biotin-11-dUTP (ThermoFisher Scientific) and 1.5 U of E.coli DNA polymerase (New England Biolabs ...
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bioRxiv - Immunology 2021Quote: ... in a final reaction volume of 20 μL using Platinum Taq High Fidelity polymerase (Invitrogen, #11-304-029) and PCR primers envB5out (5’-TAGAGCCCTGGAAGCATCCAGGAAG-3’ ...
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bioRxiv - Immunology 2021Quote: ... Reactions were formed in 20 μL reaction volume using Platinum Taq High Fidelity polymerase (Invitrogen, #11-304-029) and PCR primers 275F (5’-ACAGGGACCTGAAAGCGAAAG-3’ ...
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bioRxiv - Developmental Biology 2021Quote: ... Trypsin was inactivated with 200µl of mouse PGC culture media [DMEM/F-12 (Thermo Fisher #11-320-033), 250µM sodium pyruvate (Thermo Fisher #11360070) ...
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bioRxiv - Biochemistry 2020Quote: ... The cDNA was eluted in 11 μL ROX-HiDi and analyzed on a 3500 Genetic Analyzer (Applied Biosystems) for capillary electrophoresis ...
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bioRxiv - Immunology 2021Quote: ... The CD45-negative fraction was stained with anti-MHC class II-FITC antibodies (Invitrogen Cat#11-5321-82)(dilution 1:100) ...
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bioRxiv - Molecular Biology 2022Quote: ... INS-1 832/13 cells were cultured in 11 mM glucose RPMI 1640 (Thermo Fisher Scientific, Cat# 11875) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2021Quote: ... 1.25 NaH2PO4, 26 NaHCO3 and 11 D–glucose (pH 7.35, B303 mOsm)) containing Alexa Flour 488 (50μ, Invitrogen) (tip opening ...
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bioRxiv - Neuroscience 2022Quote: ... 11 and 20 and enriched by FACS into collection tubes containing Trizol LS Reagent (Thermo Fisher Scientific, 10296010). Total RNA from 60,000 sorted cells/sample was isolated via the precipitation method in combination with spin-columns using PureLink RNA Micro Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: Total CREB and pCREB were quantified using ELISA (85-86153-11, Instant One ELISA CREB1, Thermo Fisher Scientific) in accordance with the manufacturer’s instructions.
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bioRxiv - Systems Biology 2021Quote: ... cDNA was synthesized with 11 ul of extracted RNA supernatant using Verso cDNA kit (Thermo Scientific, Wilmington, DE) following the manufacturer’s protocol and diluted into 4-fold with nuclease-free water ...
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bioRxiv - Biophysics 2022Quote: ... the corresponding purified plasmid was first linearized by restriction digestion and biotin-labeled using Biotin-11-dUTP (ThermoFisher) and Klenow fragment (New England BioLabs) ...
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bioRxiv - Immunology 2022Quote: ... TIL were stained before seeding at day 11 of the REP with 5 μM CFSE (Thermo Fisher Scientific) for 20 min at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... and combined with 11 μL loading buffer (4X NuPAGE LDS sample buffer, Thermo Fisher, containing 10% β-mercaptoethanol). The sample was heated at 95°C for 5 min and loaded into a 4-12% Criterion XT Bis-Tris Protein Gel ...
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bioRxiv - Cancer Biology 2022Quote: ... and with LIN (anti-mouse TER119-FITC, Thermo Fisher, 11-5921-82; anti-mouse CD31-FITC, Thermo Fisher, 11-0311-85 ...
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bioRxiv - Cancer Biology 2022Quote: ... overnight at 4 °C and followed by staining with FITC- streptavidin (Invitrogen, 11-4317-87, 1:400 dilution) for 1 h at RT ...
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bioRxiv - Systems Biology 2023Quote: ... 100 μL fractions were collected between minutes 11 to 30 into 96 Well DeepWell Polypropylene Microplates (Thermo Scientific).
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bioRxiv - Cell Biology 2023Quote: ... anti-mouse integrin β1 (Armenian hamster monoclonal, Hmb1-1, Thermo Fisher Scientific, 11-0291-82; FC 1:300), anti-mouse integrin β3 (armenian hamster monoclonal ...
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bioRxiv - Biochemistry 2023Quote: Gene coding for human phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 PH domain 11-140aa (PLCδ, Acc# P51178.2) was synthesized by GeneArt (Invitrogen) as a codon optimized open reading frame ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The reaction was stopped using Stop Reagent (1:11, in 1XPBS, Alexa Fluor 594 Tyramide SuperBoost Kit, Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: 2×104 DCs were stimulated with LPS (100ng/mL) in glucose free RPMI (11-879-020, Fisher Scientific). After 1 h ...
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bioRxiv - Bioengineering 2023Quote: ... media was changed to OPTI-MEM I reduced serum media without phenol red (Fisher Scientific, 11-058-021). After 48 hours ...
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bioRxiv - Neuroscience 2023Quote: Batches 1 and 2 were labeled using the TMT 11-plex kit (ThermoFisher 90406 – Lot# TG273545 and TG273555) while batch 3 was labeled with TMTPro (ThermoFisher A44520 – Lot# VH311511 ...
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bioRxiv - Biochemistry 2023Quote: ... The following antibody cocktail was used: mCD3 (FITC, clone 17A2, Invitrogen Cat No.11-0032-80, 1:100) and mCD19 (APC ...
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bioRxiv - Bioengineering 2023Quote: ... for 1 day at a density of 4.16e4 cells/cm2 (4.16e5 cells/well) in expansion media consisting of high glucose Dulbecco’s modified Eagle’s medium (11-995-081; Gibco), fetal bovine serum (S11550 ...
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bioRxiv - Immunology 2024Quote: ... Serum was obtained and the Th1/Th2 Cytokine 11-Plex Mouse ProcartaPlex™ Panel (Invitrogen, EPX110-20820-901) was used according to the manufacturer’s instructions to quantify cytokine levels.
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bioRxiv - Microbiology 2024Quote: ... gondii polyclonal (in-house) with 1:750 goat anti-mouse Alexa 594 secondary antibody (Thermo Scientific 11-005). Secondary antibodies and DAPI were incubated 1 hour at RT ...