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Citations for Thermo Fisher :
451 - 500 of 6145 citations for GDF 11 BMP 11 Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Both HEK293T and A549 cell lines were maintained at 37°C and 5% CO2 in high-glucose DMEM (11-965-092, Gibco), supplemented with 10% fetal bovine serum (Genclone ...
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bioRxiv - Microbiology 2021Quote: ... Calu-3 bronchial adenocarcinoma cells (ATCC ® HTB-55™) (11) were cultured in DMEM-F12 (1X) (Thermo-Fisher Scientific) supplemented with 10% FBS and 1% penicillin-streptomycin ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained (10 μL/1.5×105 cells) with anti-IL-1 alpha (Thermo Fisher Scientific; Cat.# 11-7118-81) and anti IgG1 kappa (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed with 11 % glutaraldehyde and subsequently Hoechst-stained (10 mg/ml stock in 1× PBS, 1:2500; Invitrogen) using a Tecan Evo 200 robotic platform ...
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bioRxiv - Cell Biology 2021Quote: ... 60 picomoles siRNA and 11 μl of RNAiMAX were diluted separately in 250 μl of Opti-MEM (Gibco#11058-021). These diluted solutions were mixed together ...
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bioRxiv - Biophysics 2022Quote: Purified SN25 FL or 11–206 (E55W/R59C) was labeled with 10× molar excess monobrombimane (mBBr, Molecular Probes; Eugene, OR) in buffer 25 mM HEPES pH 7.4 ...
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bioRxiv - Cancer Biology 2022Quote: The transfection of siRNA into MOLM-14 and MV4-11 cells was performed using the Neon transfection system (Life Technologies) according to the manufacturer’s recommendations (1700V ...
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bioRxiv - Microbiology 2019Quote: ... The complete protocol can be found in Virus Host Interactions Methods and Protocols (30). Low passage NHDF cell suspension (approx. passage 11) were reverse-transfected with siRNA and Lipofectamine RNAiMAX (Invitrogen) using MultiDrop 384 ...
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bioRxiv - Immunology 2020Quote: ... Total RNA (1 μg) was reversed transcribed to cDNA using SuperScript™ VILO™ Master Mix (Invitrogen, 11-755-050).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Two assay media were employed for the analysis of parasites: RPMI 1640 medium ((Thermo Fisher Scientific, containing glucose 11 mM) and Seahorse XF RPMI Medium (Agilent Technologies) ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... The first injection contained either vehicle (0.9% saline) or the 11-beta hydroxylase inhibitor metyrapone (20 mg/kg) (Fisher Scientific) in saline solution ...
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Immunoresolvents Support Skeletal Myofiber Regeneration via Actions on Myeloid and Muscle Stem CellsbioRxiv - Immunology 2020Quote: ... Total RNA (1 μg) was reversed transcribed to cDNA using SuperScript™ VILO™ Master Mix (Invitrogen, 11-755-050) and RT-PCR performed on a CFX96 Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Bioengineering 2021Quote: ... All plates were spread with 150uL of the yMM1456 (suc2Δ leu2Δ) strain with glass beads (Fisher Scientific, #11-312B 4mm), the beads were removed and the plate allowed to dry for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... Proteins were detected on a Li-Cor Odyssey Blot Imager using an anti-Caspase-11 primary antibody (cone 17D9) and Alexfluor-680 conjugated secondary antibody (Invitrogen).
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bioRxiv - Systems Biology 2023Quote: ... then eluted by incubation for 20 min in 11 µL Formamide-ROX350 mix (1000 µL Hi-Di Formamide (Thermo Fisher) and 8 µL ROX350 ladder (Thermo Fisher) ...
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bioRxiv - Neuroscience 2022Quote: ... 20 three months old transgenic TgN3R182C150 mice were immunized initially either with 25 µg of aggregated NOTCH3 EGF1-5 protein (n=11, vaccinated) plus adjuvant (Imject Alum Adjuvant, ThermoFisher) or PBS (n=9 ...
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bioRxiv - Cell Biology 2022Quote: Utricles from vestibular schwannoma patients or organ donors were placed in DMEM/F12 (Thermo Fisher Scientific/Gibco, 11-039-021) with 5% FBS during transport to the laboratory ...
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bioRxiv - Cell Biology 2022Quote: Utricles from vestibular schwannoma patients or organ donors were placed in DMEM/F12 (Thermo Fisher Scientific/Gibco, 11-039-021) with 5% FBS during transport to the laboratory ...
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bioRxiv - Cell Biology 2023Quote: ... The Isolated islets were hand-picked and cultured in complete media (RMPI-1640 containing 11 mM glucose, 10% FBS, 0.1% Penicillin/Streptomycin, gentamicin and Amphotericin B; Thermo Fisher) and maintained at 37°C in 5% CO2 environment to allow their recovery.
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bioRxiv - Neuroscience 2023Quote: C26 cells were cultured in complete growth medium consisting of RPMI 1640 medium with Glutamine (#11-875-093; Thermo Fisher) containing 10% of heat-inactivated Fetal Bovine Serum (FBS ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were plated 24 hours before transfection in a cell-culture treated 6-well plate (Fisher Scientific, 14-832-11). To transfect ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... incubated after for 2’ at RT with Stop Reagent (1:11, in 1XPBS, Alexa Fluor 488 Tyramide SuperBoost Kit, goat anti-rabbit IgG, Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... and resuspended in 150 µL of an antibody cocktail containing 1:200 anti-Ter119 (FITC; Thermo Scientific, 11-5921-82), 1:50 anti-CD31 (FITC ...
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bioRxiv - Molecular Biology 2023Quote: ... Another digestion was conducted using 1000 U/mL collagenase type 2 and 11 U/mL dispase (Gibco, Cat# 17105-041) for 30 min ...
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bioRxiv - Bioengineering 2023Quote: ... Purity of gDNA was assessed using either a Denovix DS-11 spectrophotometer or the Qubit 3 fluorometer (Thermo Fisher Scientific) and dsDNA Broad Range Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... siRNA-mediated depletion of endogenous ESCO2 was done with 20nM siRNA (Dharmacon, J-025788-11, target nucleotides: GUUGAUACCCUCAGGAAUU) in serum-free Opti-MEM (Gibco) 24 hours after induction ...
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bioRxiv - Cancer Biology 2024Quote: ... were stained with 1µM (MOLM-13 and MV-4-11) or 3µM (HL-60) CellTrace Far Red (Thermo Fisher Scientific) to monitor cell divisions ...
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bioRxiv - Bioengineering 2024Quote: ... At day 11 a glucose starvation was carried out by replacing the medium with RPMI 1640 without glucose (Life Technologies), supplemented by B27 with insulin for 3 days ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 T-Rex (referred to as HEK293, cat.no. R71007, Invitrogen) and NIH3T3 cells were grown in DMEM Glutamax® (Thermo Fisher Scientific ...
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Modified N-linked glycosylation status predicts trafficking defective human Piezo1 channel mutationsbioRxiv - Biophysics 2020Quote: ... HEK293S GnT1-/- or HEK293 cells (ThermoFisher Scientific, Cat. No. R78007) using Lipofectamine 3000 transfection reagent (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: HEK293 cells (Invitrogen) were grown in DMEM (1g/L glucose ...
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bioRxiv - Synthetic Biology 2022Quote: HEK293 cells (ThermoFisher; further authenticated by assessing cell morphology and growth rate ...
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bioRxiv - Biophysics 2021Quote: ... DNA quality was determined by 260nm/230nm and 260nm/280nm ratios on a DS-11+ spectrophotometer (DeNovix) and concentration was determined using the Qubit 3 (Thermo Scientific). Pooled samples were sent to GeneWiz where they were analyzed by TapeStation (Agilent Technologies ...
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bioRxiv - Neuroscience 2019Quote: ... was incubated with 7μl Mouse monoclonal anti-HA.11 (Ascites Covance MMS-101R) and 20μl Rabbit polyclonal anti-HA (Invitrogen cat#71-5500) at 4°C while rotating ...
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bioRxiv - Genomics 2021Quote: ... The enriched libraries were diluted to a final concentration of 11 pM and subjected to sequencing from a single end in a Ion Proton Sequencer (Life Technologies). FASTQ files were mapped to the UCSC mouse reference genome (build mm9 ...
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bioRxiv - Microbiology 2020Quote: ... 11 μL RNA samples were used for reverse transcription by using the SuperScript™ IV First-Strand Synthesis System (ThermoFisher Scientific) with random hexamer primers ...
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bioRxiv - Immunology 2022Quote: VeroE6-eGFP were cloned and validated in-house as previously described.11 Cells were cultured and maintained in Dulbecco’s modified Eagle medium (DMEM; Gibco, Waltham, USA) supplemented with 10% (vol/vol ...
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bioRxiv - Biophysics 2022Quote: ... in 20 mM CAPS pH 11 was added and mixed thoroughly by pipetting to initiate phase separation and the plate was sealed (Thermo Scientific). ThT fluorescence was read at 10-minute intervals for 24 hours at 25°C using a Tecan Infinite M200 plate reader (Tecan Trading AG) ...
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bioRxiv - Biophysics 2022Quote: ... and SN25 FL (1–206, R59C) or truncation mutant (11–206, R59C) were labeled with 5×molar excess Tetramethylrhodamine-5-maleimide (TMR) (Molecular Probes) in 25 mM HEPES pH 7.4 ...
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bioRxiv - Neuroscience 2020Quote: ... All 27 samples from 3 groups and 6 GIS were divided and labeled using two sets of 5 mg 11 plex TMT reagents (Thermo Scientific A34808 ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 hr at 4°C, then proceeded for immunoprecipitation with specific antibodies (Rab5A, Rab 11 and Rab7) and Protein A/G bead (Thermo Fisher) for overnight at 4°C ...
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bioRxiv - Genomics 2022Quote: ... followed by a 1:100 dilution of Anti-mouse CD45 antibody conjugated to FITC (Thermo Fisher Scientific, Cat#: 11-0451-81) and 1:100 dilution of Anti-mouse EpCAM antibody conjugated to eFluor450 (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2019Quote: ... but only 21 out of 24 adults (11 from D1 and 10 from HP population) had enough DNA remaining to be quantified using NanoDrop® (ThermoFisher). DNA samples were normalized (when necessary ...
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bioRxiv - Microbiology 2019Quote: ... A stable line expressing the recombinant protein was generated by co-transfection of pIEX-10: Pbc43-SP/TM and pIEX-10:Neo plasmid [11] using the Cellfectin® II Reagent (ThermoFisher) according to the manufacturers’ guidelines ...
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bioRxiv - Systems Biology 2021Quote: ... Aliquots of 15 μg of total protein extract from each sample (3 strains x 4 conditions x 3 replicates) and the three bulks were separated on onedimensional SDS-PAGE short-migration gels (11×1 cm lanes, Invitrogen, NP321BOX). Yeast proteins digestion was performed on excised bands from gel gradient and digested peptides of UPS2 (Sigma ...
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bioRxiv - Immunology 2021Quote: ... Total RNA (250 ng) was then reverse transcribed to cDNA using SuperScript™ VILO™ Master Mix (Invitrogen, 11-755-050) and RT-qPCR performed on a CFX96 Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Biochemistry 2019Quote: MV4-11 and AML193 cells were obtained from ATCC and were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM) (ThermoFisher Scientific, Waltham, MA) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biochemistry 2021Quote: ... were prepared for mass spectrometry using the Preomics iST-NHS (Preomics, catalogue # P.O.00026) and TMT 11-plex labelling (ThermoFisher, catalogue # A37725) kits according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... the gypsy backbone used in previous publication (11) was amplified and cloned into the NotI/XbaI sites of pAc5.1/V5-His version B (V411020, Thermo Fisher Scientific). To synthesize the final pAc-gypsy-H2B-mCherry vector ...
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bioRxiv - Immunology 2021Quote: ... First-strand cDNA synthesis was performed on 11 μl of total RNA using 10 pmol of each primer in a 20ul total reaction (SuperScript III, Thermo Fisher) using the manufacturer’s protocol ...