Labshake search
Citations for Thermo Fisher :
551 - 600 of 10000+ citations for 1 Benzyl 3 Tert Butyldimethylsilyl Oxy Methyl Piperidin 4 One since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... RT-PCR was performed using OC43-nucleocapsid specific TaqMan primers and a probe 18 fluorescent- labelled with a 5’-FAM reporter dye and 3’-BHQ quencher (IDT) and AgPath-ID™ One-Step RT-PCR kit (AgPath AM1005, Applied Biosystems) on an ABI QuantStudio 3 platform (Thermo Fisher) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... situated between nsP4 and the viral 3’ untranslated region (UTR) were reverse-transcribed and PCR-amplified using the SuperScript™ IV One-Step RT-PCR System (ThermoFisher, #12594025) and the 26S-F primer and pooled SinRev primer ...
-
bioRxiv - Cancer Biology 2023Quote: ... 3 × 105 cells were seeded in one well of a 12-well plate on day 0 and counted on day one until day 3 with cell counters (Thermo Fisher Scientific). For colony formation ...
-
bioRxiv - Immunology 2021Quote: ... was added as the secondary antibody at a 1:2000 dilution for 1 h at 37C, followed by adding TMB (3, 3, 5, 5’-tetramethylbenzidine) peroxidase substrate (Thermo Scientific) for about 15 min ...
-
bioRxiv - Immunology 2024Quote: ... slides were washed with 1:3 diluted blocking buffer (3-times, 1 minute) and incubated with anti-mouse Alexa fluor-594 antibody (Invitrogen, A21203), anti-rabbit Alexa fluor-750 (Invitrogen ...
-
bioRxiv - Cell Biology 2021Quote: ... 5 L-malic acid and 20 μM tetramethylrhodamine methyl ester (TMRM, Thermo Fisher) for 10 minutes ...
-
bioRxiv - Neuroscience 2021Quote: ... Fly brains were incubated with 100 nM Tetramethylrhodamine methyl ester (TMRM) (Invitrogen, I34361) for 30 min at 37 °C and washed three times with Hank’s balanced salt solution (HBSS) ...
-
bioRxiv - Molecular Biology 2020Quote: ... cells were loaded with 10 nM Tetramethyl Rhodamine Methyl Ester (TMRM) (Molecular Probes) and incubated at 37°C for 30 min ...
-
bioRxiv - Genomics 2020Quote: ... and the mitochondria-specific dye tetramethylrhodamine methyl ester (TMRM, 25nM; Thermo Fisher Scientific) diluted in Hanks solution (Gibco ...
-
SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... Cells were incubated with 15 nM tetramethyl rhodamine methyl ester (TMRM; #T668; Invitrogen) in culture medium for 25 min at 37°C and 5% CO2 in the dark ...
-
bioRxiv - Cell Biology 2023Quote: ... FM4-64 and leucyl-L-leucine methyl ester (LLOMe) were from Thermo Fisher Scientific ...
-
bioRxiv - Physiology 2023Quote: ... and subsequently alkylated with 50 mM S-methyl methanethiosulfonate (MMTS) (Thermo Fisher Scientific) in USS for 1 h at room temperature ...
-
bioRxiv - Cell Biology 2024Quote: ... MitoTracker Red CMXRos and TMRM (Tetramethylrhodamine, methyl ester) (Molecular Probes, Invitrogen, Paisley, UK) were used to assess mitochondrial membrane potential (ΔΨm) ...
-
bioRxiv - Cell Biology 2024Quote: ... MitoTracker Red CMXRos and TMRM (Tetramethylrhodamine, methyl ester) (Molecular Probes, Invitrogen, Paisley, UK) were used to assess mitochondrial membrane potential (ΔΨm) ...
-
bioRxiv - Cell Biology 2024Quote: Mitochondrial membrane potential was visualized with tetramethylrhodamine methyl ester (TMRM; Thermo Fisher Scientific) as described previously (Steinberg et al. ...
-
bioRxiv - Genetics 2020Quote: ... RNA was quantified using a NanoDrop™ One (#ND-ONE-W, ThermoFisher Scientific) and 2 μg per sample were used for cDNA synthesis by performing a retro-transcription reaction with the SuperScript™ VILO™ cDNA Synthesis Kit (#11754050 ...
-
bioRxiv - Microbiology 2021Quote: ... as measured by NanoDrop One/One Microvolume UV-Vis Spectrophotometer (Thermo Fisher Scientific) was loaded onto a 50-cm μPAC capLC column (PharmaFluidics ...
-
bioRxiv - Microbiology 2022Quote: NanoDrop One Microvolume UV-Vis spectrophotometer (Thermo Fisher Scientific, catalog #: ND-ONE-W).
-
bioRxiv - Cancer Biology 2021Quote: ... RNA purity was assessed using a NanoDrop One (ThermoFisher Scientific, ND-ONE-W). The RNA samples (1μg ...
-
bioRxiv - Microbiology 2022Quote: ... One infected and one uninfected flask were treated with DSS Crosslinker (Thermo Scientific) following manufacturer instructions for intracellular crosslinking ...
-
bioRxiv - Biophysics 2022Quote: ... RNA was then quantified using a Nano Drop One (Thermofisher #ND-ONE-W) and the concentration adjusted to 60 ng/uL ...
-
bioRxiv - Cell Biology 2024Quote: ... Protein concentrations were determined using a NanoDrop One (Thermo Fisher, ND-ONE-W) using absorbance.
-
bioRxiv - Immunology 2023Quote: ... the IgG concentration was determined using NanoDrop One (Thermo Fisher, ND-ONE-W). All monoclonal antibodies were assessed for integrity by SDS-PAGE analysis.
-
bioRxiv - Physiology 2023Quote: ... One hundred microliters of One-step Ultra TMB ELISA substrate (Thermo Scientific #34028) was added to each well ...
-
bioRxiv - Cancer Biology 2021Quote: Total RNA was isolated from gastrocnemius muscles of experimental mice (n=4 BC-PDOX; n=4 BC-PDOX PIO; n=3 NSC-Con) using Trizol (ThermoFisher Scientific, Waltham, MA, USA) and established methods21 ...
-
bioRxiv - Microbiology 2020Quote: ... and resuspended in 0.1 ml of PBS supplemented with the lipophilic styryl membrane dye N-(3-triethylammoniumprpyl)-4-(p-diethylaminophenyl-hexatrienyl) pyridinium dibromide (FM4-64, Molecular Probes, Invitrogen; 10 μg.ml-1) (Pogliano et al. ...
-
bioRxiv - Neuroscience 2022Quote: ... brain slices were washed (5 min at RT x 3 times) in PBS and incubated (overnight at 4°C) with streptavidin-Alexa 488 (ThermoFisher Scientific, S-11223, 1:500) in PBS-triton 0.05% ...
-
bioRxiv - Physiology 2022Quote: ... The samples were then mixed with 1 mL of a 3:13 solution of Ehrlich’s reagent (1.5 g of 4-[dimethylamino] benzaldehyde [ThermoFisher]; 5 mL ethanol; 337 µL sulfuric acid) to isopropanol and incubated for 30 min at 58°C ...
-
bioRxiv - Neuroscience 2020Quote: ... through one channel of each tetrode and subsequent transcardial perfusion with saline followed by 10% buffered formalin (SF100-4, ThermoFisher Scientifc). Brains were extracted and stored in fixative for one day ...
-
bioRxiv - Neuroscience 2021Quote: ... The designated samples were then subjected to one-dimensional gel electrophoresis (1DE) employing precast NuPAGE 4-12% Bis-Tris 10-well mini protein gels (Invitrogen, Germany) with 2-[N-morpholino]ethanesulfonic acid (MOPS ...
-
bioRxiv - Molecular Biology 2021Quote: ... overnight at 4 °C on rotation and for one additional hour with mixed Dynabead protein G and A (20:10 µl, Life Technologies). Beads were washed with TSE- 150 (0.1% SDS,1% Triton ...
-
bioRxiv - Microbiology 2021Quote: Fifty microliters containing 3.5 x 106 PFU/ml SARS-CoV-2 viral stock was placed on one well of a 4-well chambered glass slide (Nunc, Sigma) (Fig 2) ...
-
bioRxiv - Neuroscience 2024Quote: ... were added and incubated for 4 hours at 4°c followed by one wash with lysis buffer (Pierce) with added Halt protease and phosphastase inhibitors (Thermo Scientific) and two washes with PBS and resuspended in PBS for Mass spectrometry.
-
bioRxiv - Biochemistry 2023Quote: ... A drop of 3.5-4 µl sample was applied on one side in a Vitrobot (Vitrobot Mk IV, Thermo Fisher Scientific) at 4 °C and 100 % humidity ...
-
bioRxiv - Microbiology 2023Quote: We diluted freshly purified virus samples in DPBS and combined three volumes of the virus with one volume of NuPAGE LDS Sample Buffer (4×; Thermo Fisher) containing 100 mM dithiothreitol ...
-
bioRxiv - Microbiology 2024Quote: ... unpermeabilised cells were blocked with 2% FBS/PBS for one hour with immunostaining performed as above with the inclusion of 4′,6-diamidino-2-phenylindole (DAPI, Thermo Fisher) [1:1000].
-
bioRxiv - Systems Biology 2024Quote: ... After incubation for one hour on a rotator and 4 °C in the low protein binding microcentrifuge tubes (1.5 mL, Thermo Scientific™), the supernatant containing the non-bound library fraction was discarded and the magnetic beads with the bound fraction were washed in 1 mL of the SH2 binding buffer containing Tween 20 for 30 minutes on a rotator and 4 °C ...
-
bioRxiv - Bioengineering 2024Quote: Live and dead cells were stained with 1.6-4 mM calcein AM (C125400; TRC) and 4 mM ethidium homodimer-1 (EthD-1 L3224; ThermoFisher), respectively ...
-
bioRxiv - Cell Biology 2021Quote: ... N-hydroxysulfosuccinimide (sulfo-NHS) and 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) from Thermo Scientific were dissolved in 18 MOhm DDW immediately before use ...
-
bioRxiv - Bioengineering 2022Quote: ... 1-ethyl-3-(−3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) was purchased from Fisher Scientific (Pittsburgh, PA, USA). 2-Bromo-2-methylpropionic acid (BMPA) ...
-
bioRxiv - Neuroscience 2024Quote: Rabbit monoclonal or polyclonal antibodies include those against 14-3-3 (Invitrogen, 51-0700, 1:1000); clathrin heavy chain (Abcam ...
-
bioRxiv - Neuroscience 2024Quote: ... NIM was replaced to 3:1-medium consisting of 3 parts DMEM (Thermo Fisher Scientific, #31966047) and one-part F12 medium (Ham’s F-12 Nutrient Mix ...
-
bioRxiv - Developmental Biology 2024Quote: 3 dpf larvae were incubated in 3 µM FM™ 1-43 Dye (Thermofisher catalog # T3163) for 2-4 minutes ...
-
bioRxiv - Cancer Biology 2021Quote: ... U251 cells were treated with 4 uM CellEvent Caspase-3/7 green detection reagent (Molecular probes) prior to imaging ...
-
bioRxiv - Developmental Biology 2021Quote: ... cDNA was generated using either the Superscript 3 or 4 First Strand Synthesis System (Thermo Fisher Scientific ...
-
bioRxiv - Bioengineering 2021Quote: ... pET15b-CDA5 was amplified using primers 3 and 4 and added to the BsiW1 (Thermo Fisher) site of pSLIK using InFusion (Takara ...
-
bioRxiv - Neuroscience 2021Quote: ... Nucleus staining was performed using 4’,6-diamidino-2-phenylindole (DAPI) (3 mM, D3571, Molecular Probes). Cells were counted from four randomly selected fields per culture under a confocal microscope (TCS SP8 ...
-
bioRxiv - Biochemistry 2022Quote: To prepare samples a 3:4 dilution was made in NuPAGE LDS sample buffer (ThermoFisher; NP007) supplemented with 10% v/v β-mercaptoethanol ...
-
bioRxiv - Cell Biology 2022Quote: ... 10% glycerol) were separated on 3-8% Tris-acetate or 4-12 Bis-Tris gels (Thermofisher). Proteins were then transferred to PVDF membranes (Merck).
-
bioRxiv - Microbiology 2020Quote: ... All PBMCs were stimulated for 3-4 days with 2ug/ml of phytohemagglutinin (PHA; Gibco BRL) and 1 ug/ml of interleukin-2 (IL-2 ...