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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for 1 Benzyl 3 Tert Butyldimethylsilyl Oxy Methyl Piperidin 4 One since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 1% 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Gibco) and maintained at 28°C in the absence of CO2 ...
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bioRxiv - Molecular Biology 2021Quote: ... counted and reseeded in 3 mL A medium (1:3 mix DMEM/F12 (Gibco) and Neurobasal (Gibco) ...
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bioRxiv - Bioengineering 2023Quote: ... and EDC (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... EVD68 2C NΔ39: εmax = 20,860 M-1·cm-1) with NanoDrop One (Thermo Fisher Scientific Inc.). The ionic strength of the dialyzed protein solution was confirmed with a conductivity meter ...
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bioRxiv - Microbiology 2022Quote: ... pTG-Luc and SARS2-COV2 spike expression vector were transfected into HEK 293T cells at the ratio of 3:4:3 using Lipofectamine 3000 transfection reagent (ThermoFisher Scientific, Waltham, MA). Accession IDs of the spike proteins used in the study were ...
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bioRxiv - Bioengineering 2024Quote: ... and a lentiviral transfer plasmid (2:3:4 ratio by mass, 3 µg total plasmid) using Lipofectamine 3000 (Thermo Scientific cat. no. L3000015). Media was exchanged after 6 hours and viral supernatant was harvested 48 hours after transfection and filtered through 0.45 μm cellulose-acetate filters (VWR cat ...
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bioRxiv - Molecular Biology 2021Quote: Tert-immortalized keratinocytes (kindly provided by Jerry Shay, UT-Southwestern) were cultured in EpiLife Medium (catalogue # MEPICFPRF500, Thermo Fisher Scientific) supplemented with human keratinocyte growth supplement (catalogue #S-001-5 ...
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bioRxiv - Genetics 2024Quote: ... Wild type N/TERT-2G cell line was cultured using EpiLife medium with 60 µM calcium (Gibco™, cat. #MEPI500CA), supplemented with human keratinocyte growth supplement (HKGS ...
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bioRxiv - Microbiology 2024Quote: ... The Na+-specific ionophore monensin (Solarbo, China) and the protonophore 3,5-di-tert-butyl4-hydroxybenzaldehyde (DTHB, Thermo Fisher Scientific, USA) were used to test which pathway drives ATP synthesis ...
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bioRxiv - Cell Biology 2020Quote: ... Tetramethylrhodamine methyl ester (TMRM, cat n. T668, Molecular Probes, Leiden, The Netherlands) was dissolved in DMSO to obtain a 10 mM stock solution and then diluted in the appropriate buffer ...
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bioRxiv - Cell Biology 2020Quote: ... cells were co-stained with 16.7 nM tetramethylrhodamine methyl ester (TMRM; Invitrogen) and 100 nM Mitotracker Green (MTG ...
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bioRxiv - Microbiology 2021Quote: ... Cysteine alkylation was induced by addition of methyl methanethiolsulfonate (MMTS, Thermo Scientific) in 100% isopropanol to obtain a final concentration of 10 mM followed by a 10 min incubation in a thermoshaker at 37°C ...
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bioRxiv - Immunology 2021Quote: For measurement of mitochondrial membrane potential TMRM (Tetramethylrhodamine, Methyl Ester, Perchlorate; ThermoFisher) was added to the cells at 30 nM final concentration ...
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bioRxiv - Molecular Biology 2023Quote: To assess paraquat (Methyl viologen 98%, Thermo Fisher Scientific Cat. No.: 227320050) toxicity ...
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bioRxiv - Developmental Biology 2022Quote: ... using donor embryos labeled with tetramethyl rhodamine methyl ester (TMRM, Fisher Scientific). To produce contemporaneous stage-matched embryos for PMC transplants ...
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bioRxiv - Genetics 2023Quote: ... 1X cutting solution: 93 mM N-Methyl-D-glucamine (Acros Organics, AC126841000), 2.5 mM KCl (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... proteins were eluted with a 1M α-Methyl-D-mannopyranoside (Acros Organics) pH8 solution.
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... 10.6 mM Nα-tosyl-L-arginine methyl ester HCl (Fisher Scientific, T03301G), 10.8 mM tosyl-lysine-chloromethylketone HCl (Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... mixed (1:1 in volume) with Fluo 4 dye (Fluo 4 Direct Calcium Assay Kit, ThermoFisher Scientific) and incubated at 37°C for 30 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... the suspending fluid was 0.6% w/v or 0.7% w/v methyl cellulose (Thermo Scientific, molecular weight 454.513 g mol−1) in phosphate buffered saline (PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were incubated in 2 μM JC-1 (5, 5’, 6, 6’-tetrachloro-1, 1’, 3, 3’-tetraethylbenzimidazolylcarbocyanine iodide, Invitrogen, cat no: T3168) at 37°C for 15 min ...
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bioRxiv - Biochemistry 2021Quote: ... Stained ovaries were finally mounted with one drop of vectashield in epoxy diagnostic slides (Thermo-Fisher Scientific, 3 wells 14 mm) and covered with high precision cover glasses ...
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bioRxiv - Microbiology 2024Quote: ... isolated RNA was run using a TaqMan Fast Virus One-Step Master Mix (applied Biosystems) on a QuantStudio 3 Flex Real-Time PCR system (Applied Biosystems). RNA standards with known copy numbers were used to generate a standard curve and calculate sample copy numbers ...
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bioRxiv - Cell Biology 2024Quote: ... was then used for qPCR analysis to evaluate the binding to 3’UTR and Meril One-Step qRT-PCR Kit (Thermo Fisher) for E ...
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bioRxiv - Cell Biology 2021Quote: ... and one-fifth of the affinity purification (IP) were separated on Bolt 4-12% Bis-Tris Plus 15-well gels (Invitrogen), run in Bolt 1x MOPS SDS running buffer (Novex ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was eliminated from RNA extracts by digesting for one hour at 37°C with 4 U of TURBO DNAase (Ambion) followed by purification with MEGAclear Transcription Clean-Up kits (Ambion).
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bioRxiv - Microbiology 2021Quote: ... Protein purity was determined by resolving one to two micrograms of purified protein by SDS-PAGE using NuPAGE 4–12 % Bis Tris precast gels (ThermoFisher) for 50 minutes at 200 V ...
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bioRxiv - Genomics 2022Quote: ... We estimated DNA quality using a NanoDrop One Microvolume UV-Vis Spectrophotometer and quantified DNA using a Qubit 4 Fluorometer (ThermoFisher) with a Quant-iT dsDNA HS Assay Kit ...
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bioRxiv - Microbiology 2022Quote: ... Protein purity was determined by resolving one to two micrograms of purified protein by SDS-PAGE using NuPAGE 4–12% Bis Tris precast gels (ThermoFisher) for 50 minutes at 200V ...
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bioRxiv - Immunology 2023Quote: ... One to 20 μg of total protein were separated by NuPAGE™ 4-12% Bis-Tris Gel (Thermo Fisher Scientific) and transferred onto PVDF membranes (Bio-Rad ...
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bioRxiv - Bioengineering 2024Quote: ... the spent drug-containing media was removed and VICs were immersed in a 4% paraformaldehyde solution in PBS for 20 minutes then permeabilized for one hour with 0.1% Triton-X-100 (Fisher Scientific) in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... using the NanoDrop One/OneC spectrophotometer (ThermoFisher, Cat. No. ND-ONE-W) to determine gDNA concentrations ...
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bioRxiv - Genomics 2020Quote: ... Abl.3 and Abl.4 (13) were cultured in Roswell Park Memorial Institute medium (Gibco), containing 15% FBS (Sigma) ...
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Potassium channel-driven bioelectric signaling regulates metastasis in triple-negative breast cancerbioRxiv - Cancer Biology 2021Quote: ... CDH11 #4: 5’-CCUUAUGACUCCAUUCAAA-3’ using Lipofectamine RNAiMAX transfection reagent (13778030; ThermoFisher Scientific, Waltham, MA) in serum-free DMEM ...
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bioRxiv - Cell Biology 2021Quote: HAEC cells (passage 3-4) were lysed in ice-cold RIPA buffer (ThermoFisher, cat# 89900) containing protease and phosphatase inhibitors (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... washed 3 times and incubated with 4’,6-Diamidino-2-phenylindole (DAPI; 2mg/ml) (Invitrogen) in PBS for 5 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell number was determined every 3-4 days using the Countess automated cell counter (Invitrogen). 20,000 cells were then re-plated with fresh media and compound ...
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bioRxiv - Cell Biology 2020Quote: ... After washing and nuclear counterstaining with 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher, 3 µM), sections were mounted on microscopic slides using Aqua Poly/Mount (Polysciences) ...
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bioRxiv - Biochemistry 2021Quote: ... RNA was isolated from approximately 3-4 x 107 cells using TRIzol reagent (Thermo Fisher), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... for 3-4 hr in the presence of brefeldin A (BFA; 10ug/mL; Life Technologies).
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bioRxiv - Cancer Biology 2024Quote: ... 3-4 μm paraffin sections were prepared with a HM 355S microtome (Fisher Scientific, 10862110), deparaffinized and rehydrated up to 96% ethanol (v/v) ...
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bioRxiv - Immunology 2023Quote: ... Cell passaging was performed every 3 to 4 days using 0.05% Trypsin-EDTA solution (Gibco). Expi293F cells were maintained in Expi293 Expression Medium (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: Hippocampal neurons were transfected at day in vitro (DIV)3-4 using Lipofectamine 2000 (Invitrogen). Shortly ...
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Migration and establishment of progenitor pool of melanocytes is governed by SEMA3E-PLXND1 signalingbioRxiv - Developmental Biology 2023Quote: ... cells were switched to M254 medium for 3-4 population doublings (Thermofisher Scientific, Life Technologies).
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Migration and establishment of progenitor pool of melanocytes is governed by SEMA3E-PLXND1 signalingbioRxiv - Developmental Biology 2023Quote: ... cells were switched to M254 medium for 3-4 population doublings (Thermofisher Scientific, Life Technologies).
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bioRxiv - Cell Biology 2023Quote: ... followed by a second round of transfection 3-4 hours later using RNAiMax (Life Technologies) according to the manufacturer’s instructions with final siRNA concentration of 50 nM and 20 nM for Sac2 and OSBP ...
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bioRxiv - Cancer Biology 2023Quote: ... for 3-4 days before they were collected directly in TRIzol reagent (Invitrogen cat#15596026). Before collection ...
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bioRxiv - Biochemistry 2024Quote: ... Half of the culture medium was refreshed every 3-4 days with DMEM (Gibco, USA) supplemented with 10% FBS (CellMAX ...
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bioRxiv - Biochemistry 2024Quote: ... 4°C) and resolved (~80 μg/lane) in a linear 3-12% acrylamide gradient (Invitrogen). For BN-PAGE ...
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bioRxiv - Biophysics 2024Quote: ... The Ca2+-sensitive dyes Fluo-4 AM (Dojindo) and Rhod-3 AM (Thermo Fisher Scientific) were employed ...