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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for 1 Benzyl 3 Tert Butyldimethylsilyl Oxy Methyl Piperidin 4 One since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... or from ventricular tissues of various mouse hearts was minced and placed into one well of 6-well non-coated plate and cultured in 3 ml MSC medium with Normocin (Invitrogen) and Gentamicin (GIBCO).28 The media was changed daily for 3 days and then every 2-3 days ...
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bioRxiv - Developmental Biology 2022Quote: ... To-Pro-3 (1:500, Invitrogen T3605). Secondary antibodies were obtained from Jackson ImmunoResearch or Invitrogen and used at 1:200.
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bioRxiv - Biochemistry 2022Quote: ... 3) 1% Pluronic-127 (cat. # P6866, ThermoFisher) in BRB80 ...
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bioRxiv - Systems Biology 2022Quote: ... diluted 1:3 with DPBS (Life Technologies) and 25μL added to the wells ...
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bioRxiv - Bioengineering 2021Quote: ... + 1 × 10−3 M sodium pyruvate (Gibco) + 0.05 × 10−3 M 2-mercaptoethanol (Sigma)] supplemented with soluble anti-mouse CD28 (5 µg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... and Toto-3 (1:1000; Thermo Fisher) as nuclear counterstain.
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bioRxiv - Immunology 2022Quote: ... ICOS-SB436 (ISA-3, Invitrogen, 1:50), IgG-BV480 (goat polyclonal ...
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bioRxiv - Cancer Biology 2024Quote: ... 3% H2O2 (Fisher Scientific 7722-84-1) was used ...
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bioRxiv - Genetics 2023Quote: ... diluted 1:3 in 1X PBS (Gibco). Trypsin was inactivated by cell media.
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bioRxiv - Developmental Biology 2023Quote: ... or TO-PRO-3 (1:50000, ThermoFisher) for 1 hour at room temperature or overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... proteinase K (3 μg ml−1; ThermoFisher) digestion ...
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bioRxiv - Immunology 2023Quote: ... claudin-3 (1:200; Thermo Fisher Scientific), claudin-15 (1:200 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-3 L of Expi293F cells (ThermoFisher) were grown at 37°C to a cell density of 3.2×106 cells/mL in FreeStyle expression medium (ThermoFisher) ...
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bioRxiv - Cell Biology 2024Quote: ... Lipofectamine RNAiMAX (Thermo Scientific, 13778030, 1:3), FuGene HD (Promega ...
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bioRxiv - Genetics 2022Quote: ... and washed 3-4 times with fresh 10% RNA later solution (Thermo Fisher Scientific). After the last wash ...
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bioRxiv - Zoology 2022Quote: ... The excised tissues were then placed (3-4 pieces) on gelatin (Attachment Factor, ThermoFisher)-coated dish (35 mm ...
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bioRxiv - Molecular Biology 2021Quote: ... 3-4 μl of samples were applied onto grids using a Vitrobot (Thermo Fisher). Grids were blotted with “595” filter papers (Ted Pella ...
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bioRxiv - Genetics 2021Quote: Calu-3 cells were mock transfected with 4 μL of lipofectamine 3000 (ThermoFisher Scientific) in Opti-MEM (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: FOs from 2 or 3 independent differentiations were fixed using 4% paraformaldehyde (Thermo Scientific) in PBS (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... and resolved on 3-8% or 4-12% gradient SDS-PAGE gels (Invitrogen, NuPAGE). After transfer to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2024Quote: ... The medium was replaced after 3-4 hr with neurobasal medium (Gibco, Cat# 21103049) supplemented with 2% v/v B27 (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... cells were washed 3 times with DMEM and fixed with 4% paraformaldehyde (Thermo Fisher) at room temperature (RT ...
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bioRxiv - Molecular Biology 2023Quote: ... with 10% FBS and split every 3-4 days using TrypLE Express (Thermo Scientific). Low-passage ...
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bioRxiv - Microbiology 2023Quote: ... sonicated via a Sonic Dismembrator 100 (Fisher Scientific, setting 3, 15 s, 4 pulses), and incubated on ice for 1 hr after the addition of 2 μL benzonase (EMD Millipore) ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were passaged every 3-4 days using 0.25% Trypsin-EDTA (Fisher Scientific) incubated at 37°C for 3-5 minutes to detach cells ...
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bioRxiv - Neuroscience 2024Quote: ... for 3 h at 4°C and then with protein G magnetic beads (ThermoFisher) at 4°C for an additional 2 h to obtain immunoprecipitated RNA fragments ...
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bioRxiv - Genomics 2024Quote: ... were treated 3-4 times with 50 U of T4 PNK (Thermo Fisher Scientific) and 100 U of λ-exonuclease (Thermo Fisher Scientific) ...
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A nepenthesin insert allosterically controls catalysis in the malaria parasite protease plasmepsin VbioRxiv - Microbiology 2022Quote: ... Lysates were then incubated for one hour at 4°C with Dynabeads-Protein A or Dynabeads-Protein G (ThermoFisher) that had been bound to anti-GFP (clone 3E6 ...
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bioRxiv - Immunology 2020Quote: ... One million cells from lung cell suspension were resolved on 4 – 12 % NuPAGE Bis-Tris protein gels (Thermo Fisher), then transferred onto a nitrocellulose membrane (Biorad ...
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Drosophila immune priming to Enterococcus faecalis relies on immune tolerance rather than resistancebioRxiv - Immunology 2022Quote: ... Samples were centrifuged at 10,000 rcf at 4°C for one minute directly into a 1.5mL microcentrifuge tube containing 350uL TriZol (Life Technologies) (schematic in Supplementary Figure 4A) ...
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bioRxiv - Molecular Biology 2024Quote: ... enteroids were pelleted and washed one time with 1X PBS and fixed in 4% (w/v) PFA (Fisher Scientific) for 15 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... followed by one-hour incubation with Hoechst (Life Technologies, H3570, 1:1000) and several PBS washes at RT ...
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bioRxiv - Neuroscience 2024Quote: ... were plated 1 week prior and treated with culture ONE supplement (Gibco) 2 days prior ...
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bioRxiv - Molecular Biology 2024Quote: ... one million cells were cross-linked with 1% formaldehyde (Thermo Scientific 28906) at room temperature for 10 min with slow rotations ...
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bioRxiv - Cancer Biology 2022Quote: ... PDCA-1 (ThermoFisher # 53-3179-4), TLR9 (Biolegend #394803) ...
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bioRxiv - Pathology 2023Quote: ... Fluo-4-AM (1 µM; Invitrogen), in the dark for 20 minutes at room temperature in Mg2+-PSS ...
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bioRxiv - Neuroscience 2023Quote: ... (1:300; 4 h, RT, Invitrogen) were used as secondary antibodies ...
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bioRxiv - Bioengineering 2024Quote: ... OCT-4 (701756, Invitrogen, 1:1000), TRA-1-60 Podocalyxin (14-8863-82 ...
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bioRxiv - Neuroscience 2022Quote: ... We incubated overnight with the anti-GFP primary antibody at 4 degrees (1:1000, A-6455 Invitrogen, 0.1M PBS 0.3% tx100, 3% NGS). After abundant PBS washes ...
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bioRxiv - Genomics 2022Quote: ... Plasmids were mixed in a 3:1 weight ratio of total plasmid DNA:PEI in 4 mL of either OptiMeM™ (Gibco cat. # 31985-062) or serum-free DMEM per flask and then shaken vigorously for 30 sec ...
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bioRxiv - Microbiology 2020Quote: ... Cells were infected in a BSL-3 lab with the UF-1 strain of SARS-CoV-2 at MOI of 4 in media containing 3% low IgG FBS (Fisher Scientific, Cat. SH30070.03).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... and lentiviral vector expressing GFP-UMOD-WT or GFP-UMOD-H177- R185 del at a ratio of 3:1:4 using Lipofectamine 3000 Reagent (ThermoFisher Scientific, Waltham, MA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed 3 times with 200 µL PBS for 4 min before secondary antibodies and 1 µg/mL Hoechst (Thermo Fisher Scientific #33342) were added in 35 µL per well and incubated for 1 hour at room temperature ...
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bioRxiv - Biophysics 2024Quote: ... protein fractions were mixed with SDS-free loading buffer in a 3:1 ratio and loaded on a Novex™ 4–20% Tris-Glycine Gels using NativeMark™ Unstained Protein Standards (Invitrogen™) (range from 20 to 1236 kDa ...
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bioRxiv - Cancer Biology 2024Quote: ... and the packaging plasmids psPAX2 and pMD2.G at a ratio 4:3:1 in the presence of Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). The supernatants containing lentiviral particles were collected 48 hours after transfection ...
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bioRxiv - Neuroscience 2024Quote: ... slices were incubated at room-temperature for 3-4 hours with secondary antibody mixture containing Alexa Fluor 568 anti-mouse (1:500; Thermo Fisher Scientific, A11031) and Alexa Fluor 647 anti-chicken (1:500 ...
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bioRxiv - Immunology 2023Quote: ... The concentration was measured at NanoDrop One/One (Thermo Fisher Scientific) and the quality (RNA integrity number ...
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bioRxiv - Developmental Biology 2024Quote: ... and measured on a Nanodrop One (Thermo Fisher, ND-ONE-W) to confirm A260/A280 above 2.0 and A260/A230 values were above 1.8 ...
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bioRxiv - Molecular Biology 2021Quote: ... antibodies overnight at 4°C and then incubated for an additional 3 hours at 4°C with 50 μL DynabeadsTM protein G (Life Technologies, 10004D). Following three washes with 1% Triton-TBS lysis buffer ...