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Citations for Thermo Fisher :
5851 - 5900 of 10000+ citations for Zika Virus NS1 Protein Uganda strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The concentration of the protein was estimated using the BCA kit (Pierce BCA Protein Assay Kit - Thermo Fisher Scientific) according to the supplier’s recommendations as well as by comparing the protein on a 12.5% acrylamide Coomassie-stained SDS-PAGE gel with BSA standards ...
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bioRxiv - Synthetic Biology 2024Quote: ... Total protein content was quantified using PierceTM BCA protein assay kit as per the supplier’s protocol (Thermo Scientific, USA). For albumin ...
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bioRxiv - Molecular Biology 2024Quote: ... 15 µl (for ChIP) or 100 μl (for ChIP-seq) Dynabeads Protein A and Protein G (Thermo Fisher Scientific) were pre-incubated overnight with rotation in the presence of 5 mg/ml BSA and 3 µg (for ChIP ...
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bioRxiv - Bioengineering 2024Quote: Protein samples were diluted in PBS to 5 μM and mixed with 5000× SYPRO Orange Protein Gel Stain (Invitrogen) to final 1.25x concentration ...
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bioRxiv - Molecular Biology 2024Quote: ... and the resulting supernatant was assayed for protein concentration with a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) and then subjected to SDS-polyacrylamide gel electrophoresis on a 10% acrylamide/4% bisacrylamide gel ...
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bioRxiv - Cell Biology 2024Quote: ... Peak measurements were normalized to protein content of duplicate samples as determined by BCA protein assay (Thermo Fisher Scientific). All experiments were performed independently at least three times and a representative experiment is shown.
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bioRxiv - Neuroscience 2024Quote: ... Supernatants were collected and the total amount of proteins was calculated by the BCA Protein Assay kit (ThermoFisher Scientific) using a BSA standard curve ...
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bioRxiv - Neuroscience 2024Quote: ... the samples underwent normalization based on protein content using the BCA protein assay kit (#23225, Thermo Scientific Pierce, US) per the manufacturer’s instructions.
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bioRxiv - Biochemistry 2024Quote: ... and total protein concentrations of cleared cell lysates were measured with the PierceTM BCA Protein Assay Kit (ThermoFisher Scientific) using BSA as a standard ...
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bioRxiv - Biochemistry 2024Quote: Protein in mouse and human wound fluid samples were quantified with the BCA Protein Assay kit (Thermo Fisher Pierce) using the 96-well plate protocol recommended by the manufacturer ...
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bioRxiv - Neuroscience 2024Quote: ... Total protein levels in all lysates were quantified using PierceTM BCA Protein Assay Reagents (Thermo Fisher: #23222 and #23224). After the incubation step for the poly-GP immunoassay ...
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bioRxiv - Microbiology 2024Quote: ... The protein concentration in each sample was determined using the BCA protein assay kit (Thermo Fisher Scientific, Waltham, USA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and total protein concentrations were quantified using a Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA). Samples for gel electrophoresis were prepared by boiling in loading buffer for 10 min ...
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bioRxiv - Systems Biology 2024Quote: ... The protein concentrations in both supernatants (SOL and SDS) were determined with Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Molecular Biology 2024Quote: ... Lysate was clarified by centrifugation and total protein concentration was measured with the BCA Protein Assay (Thermo Fisher Scientific). Samples were electrophoresed and transferred to a 0.45 μm nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein were supplemented with 150mM NaCl and incubated with Protein A/G Magnetic Beads (ThermoFisher #88802) preloaded either or not with Golgin-97 antibody ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysate was clarified by centrifugation and total protein concentration was measured with the BCA Protein Assay (Thermo Fisher Scientific). Samples were electrophoresed and transferred to a 0.45-μm nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... and SARS-CoV-2 (strain UF-1) viruses were propagated in Vero-E6 cell line (ATCC® CRL-1586™) in advanced DMEM (Gibco™, 12491015; Thermo Fisher) supplemented with 10% HyClone™ Defined low antibody ...
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bioRxiv - Physiology 2020Quote: ... Different bacterial strains were cultured overnight at 30°C in TSB media under aerobic conditions (rotating 50-60rpm, Thermo Fisher Tissue Culture Rotator CEL-GRO #1640Q). ~1010 bacteria were harvested ...
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bioRxiv - Bioengineering 2022Quote: Rheological characterization of the jammed microgel ink and PAM scaffolds was performed using parallel plates geometry (20 mm diameter, 1 mm gap) mounted on strain-controlled rheometers (Thermo Scientific HAAKE MARS 40/60 Rheometer). Jammed microgels were prepared in the same way and molded to an identical geometry to the plates ...
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bioRxiv - Pathology 2023Quote: ... Xcc 8004 and Xe 85-10 strains were synthesized as gene fragments with appropriate BpiI sites (GeneArt gene synthesis by Thermo Scientific; codon-optimized for N. benthamiana) and subcloned as level 0 CDS1ns modules (in pAGM1287 ...
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bioRxiv - Plant Biology 2020Quote: ... The protein content of the supernatant was quantified using Ready-to-use Coomassie Blue G-250 Protein Assay Reagent (ThermoFisher) and Albumin Standard 23209 (ThermoFisher).
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bioRxiv - Plant Biology 2019Quote: ... GTPase activity of native and mutant Gα proteins were detected with or without an RGS protein using the ENZchek phosphate assay kit (Invitrogen) as previously reported (Roy Choudhury et al. ...
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bioRxiv - Cancer Biology 2021Quote: The cell membrane protein samples were collected using Mem-PER eukaryotic membrane protein extraction reagent kit (Thermo Scientific, 89826, MA) according to the protocol instruction ...
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bioRxiv - Biophysics 2021Quote: ... The crude lysates were cleared by centrifugation at 13,000 rpm for 15 min and protein content was analyzed by Pierce BCA protein assay (Thermo Scientific).
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bioRxiv - Cancer Biology 2021Quote: ... After clearing by centrifugation at 13,300□rpm for 10□min at 4°C the protein concentration was determined using Coomassie Plus Protein Assay (ThermoFisher Scientific). Protein concentrations were adjusted to a final concentration of 2mg/ml followed by denaturation upon addition of 4X sample buffer containing 10% Beta-mercaptoethanol and heating to 95oC for 5 minutes ...
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bioRxiv - Cancer Biology 2021Quote: Subcellular protein fractionation was performed either by using Subcellular Protein Fractionation Kit for Cultured Cells (Thermo Scientific; Cat. no. 78840) according to the manufacturer protocol or following the protocol below.
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bioRxiv - Cell Biology 2020Quote: ... The eluted proteins were separated on a 4-12% NuPAGE gel (Novex) and stained with Imperial protein stain (Thermo Scientific). The proteins of interest were excised for identification by MS.
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bioRxiv - Cell Biology 2019Quote: Cells were lyzed using RIPA buffer and proteins were quantified using the BCA method (Protein BCA Assay Kit, Thermo Fisher). The protein extracts were separeted on 12% SDS-PAGE gels (50 μg of total proteins were loaded per well ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was then incubated with appropriate antibodies for 12 h and then conjugated with dynabeads Protein A or Protein G beads for an additional 1 h SFB- or GFP-tagged proteins were immunoprecipitated with streptavidin dynabeads (Invitrogen) or GFP-Trap (Chromotek) ...
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bioRxiv - Cell Biology 2020Quote: ... Total concentration of protein in lysate was quantified using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, 23225) measured at 562 nm and compared to a standard curve ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was transferred to a new tube and total protein was quantified using EZQ® Protein Quantitation kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: Protein in testes and cultured cells were extracted using T-PER Tissue protein extraction reagent (Thermo Fisher Scientific, Waltham, Massachusetts), subjected to SDS-PAGE ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were pelleted and 50μg of protein was quantified by ionic detergent compatible Pierce 660nm Protein Assay Reagent (Thermo Fisher) in 8M Urea ...
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bioRxiv - Biochemistry 2022Quote: ... Total protein content of each pre-cleared sample was determined using Pierce bicinchoninic acid assay protein assay (Thermo Fisher Scientific). To measure luciferase activity ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations were then measured by bicinchoninic acid (BCA) assay (Micro BCA™ Protein Assay Kit, Cat # 23235; Thermo Scientific). The resulting supernatant yielded low volumes (30-40 µl of supernatant ...
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bioRxiv - Molecular Biology 2020Quote: ... The suspension was incubated at 25°C for an additional 2 hr with Protein G SepharoseTM 4 Fast Flow or DynabeadsTM Protein G (Invitrogen), pre-blocked with 1 mg/ml BSA and 0.25 mg/ml Salmon Sperm DNA ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein concentrations of the final purified samples were measured with Pierce™BCA Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... Protein samples were separated on a NuPAGE™ 4–12% Bis-Tris Protein Gel (Thermo Fisher Scientific, Bleiswijk, the Netherlands) and transferred to an Immobilon-FL PVDF membrane (Merck ...
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bioRxiv - Genomics 2019Quote: ... Soluble material was incubated with 3-5 μg of antibody bound to 50 μl protein A or protein G Dynabeads (Invitrogen) and incubated overnight at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... Protein concentration was determined relative to a BSA standard using the PierceTM BCATM Protein-Assay (Thermo Scientific, Waltham, MA, USA). Lysates were finally adjusted to a final concentration of 2 µg/µl with 4X Laemmli buffer (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... and CCL21 levels were normalized to total protein concentration of the homogenates determined using Pierce BCA protein Assay kit (ThermoFisher).
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bioRxiv - Molecular Biology 2021Quote: ... Purity was determined by SDS-PAGE and protein concentrations were determined using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). For generation of POM1 mutants ...
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bioRxiv - Genetics 2020Quote: ... gRNA/Cas9 complexes were formed by incubating 250 ng of the gRNA with 1 µg of purified Cas9 protein (TrueCut Cas9 Protein v2, Invitrogen) in OptiMEM (Invitrogen) ...
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bioRxiv - Microbiology 2019Quote: ... The lysate/antibody mixture was then incubated with either Protein A (for Flag-Riplet experiments) or Protein G Dynabeads (Invitrogen) for 2 hours ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The amount of protein in each sample was determined using a BCA protein assay Kit (Thermo Scientific, Waltham, MA, USA) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were then clarified by spinning at 14,000 rpm at 4°C and protein concentration quantified by BCA Protein Assay (23227, Thermo Scientific). Forty micrograms of proteins were denatured in boiling SDS sample buffer and resolved on 4%-20% gradient gels (5671093 ...
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bioRxiv - Microbiology 2019Quote: ... The samples were heated at 95°C for 10 minutes and centrifuged at 4°C for 10 minutes to remove potential protein aggregates before proteins were separated in gradient polyacrylamide gels (4-12%) (Invitrogen). Proteins were transferred onto nitrocellulose membranes (Whatman ...
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The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... Samples were pre-cleared with Dynabeads Protein A and Dynabeads Protein G (Catalog #10001D and #Catalog #10003D, Thermo Fisher Scientific) for 1hr at 4 °C first and then incubated with rabbit anti-PntP1 antibody (a gift of J ...
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bioRxiv - Immunology 2021Quote: Total protein concentrations in BAL fluid were detected by Pierce BCA Protein Assay Kit according to the manufacturer’s instructions (Thermo Scientific).