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Citations for Thermo Fisher :
5951 - 6000 of 10000+ citations for Zika Virus NS1 Protein Uganda strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Protein concentrations were determined by Bradford assay and equal amounts of protein were added to a slurry of Dynabeads Protein G (ThermoFisher) and p300 antibody (NM11 ...
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bioRxiv - Neuroscience 2020Quote: ... protein concentration in each sample was determined by using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, 23225). 75µg protein per condition was transferred into new microcentrifuge tubes and 5 µL of the 200 mM TCEP was added to reduce the cysteine residues and the samples were then incubated at 55°C for 1-h ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein-containing supernatants were pre-cleared for 1 h at 4°C with Dynabead® protein G (Thermo Fisher Scientific). Pre-cleared supernatants were incubated overnight at 4°C with 1:1250 dilution of Rabbit (DAIE ...
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bioRxiv - Physiology 2021Quote: ... centrifuged for 1 min at 10’000 x g and 40 μg protein was loaded onto NuPAGE Bis-Tris 10% Protein Gels (ThermoFisher #NP0301BOX), and proteins were transferred to nitrocellulose membranes (Sigma #GE10600002) ...
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bioRxiv - Microbiology 2022Quote: ... trichloroacetic acid was performed as described previously 37 and protein concentration was determined by BCA protein assay kit (Thermo scientific). Protein samples were separated by 4– 12% gradient SDS-PAGE (GenScript ...
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bioRxiv - Developmental Biology 2022Quote: ... ex vivo: 20 μg) of protein lysates as described above were resolved on 4- 12% Bis-Tris Protein gels (Invitrogen). Proteins were transferred to nitrocellulose membranes (Millipore ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... AChE activity was normalized by protein concentration as determined by a Coomassie (Bradford) protein assay kit (Thermo Scientific, Waltham, MA) and compared to 0.5% DMSO exposed samples (set at 100% activity) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... ChE activity was normalized by protein concentration as determined by a Coomassie (Bradford) protein assay kit (Thermo Scientific, Waltham, MA) and compared to vehicle-exposed samples (set at 100% activity) ...
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bioRxiv - Cell Biology 2022Quote: ... Molecular weight markers used were Novex Sharp Pre-stained Protein Standard (LC5800) or PageRuler Prestained Protein Ladder (26617) (ThermoFisher Scientific). Following SDS-PAGE ...
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bioRxiv - Cell Biology 2022Quote: ... Protein purity was determined by resolving purified protein by SDS–PAGE using NuPAGE 4–12% Bis Tris precast gels (ThermoFisher) at 200 V ...
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bioRxiv - Neuroscience 2019Quote: Whole cell protein lysates were isolated from hippocampal tissues by using M-PER mammalian protein extraction reagent (Thermo Scientific, 78501) and by adding Halt Protease Inhibitor (Thermo Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... Cell-surface proteins were purified using Mem-PER™ Plus Membrane Protein Extraction Kit (Thermo Fisher Scientific, Rockford, lL, USA). Isolated proteins were labeled with Cy3 dye and hybridized to a lectin microarray 27–35 ...
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bioRxiv - Molecular Biology 2019Quote: ... Exosomes were used in co-culture assay and also exosomal RNA and protein were extracted using the Total Exosome RNA and Protein Isolation kit (ThermoFisher) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2019Quote: The protein lysate or exosomal protein from of WT H69 and ∆huPGRN-H69 cells were prepared using M-PER mammalian protein extraction reagent (ThermoFisher) or exosome protein isolation kit (ThermoFisher ...
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bioRxiv - Bioengineering 2019Quote: ... RLU readings were normalized to total protein content as measured by Pierce BCA Protein Assay Kit (ThermoFisher Scientific; Waltham, MA). Vector genome copies were measured by qPCR (Supplementary Methods).
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bioRxiv - Microbiology 2019Quote: ... The protein concentrations of the lysates were then quantified using a bicinchoninic acid protein kit (Thermo Fisher Scientific, Rockford, IL). Aliquots containing 12 µg of total cellular lysates for each sample were denatured at 100 °C for 5 min and separated using 8% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Physiology 2019Quote: ... The protein concentration was measured with the reducing agent compatible Pierce BCA Protein Assay Kit (Thermo Scientific, Rockford, IL, USA).
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bioRxiv - Cancer Biology 2019Quote: ... Total protein in the CM was estimated using the Pierce™ BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Cancer Biology 2019Quote: ... Supernatants were collected and total protein concentration was measured using a BCA Protein Assay Kit (Thermo Scientific, Waltham, MA, USA). An equimolar amount of protein was loaded into each well and separated by 12% SDS-PAGE ...
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bioRxiv - Molecular Biology 2019Quote: ... Antibody-bound chromatin was captured with magnetic beads for 1 h at 4°C (50 μl 1:1 mixture of Dynabeads Protein A and Dynabeads Protein G, Invitrogen). DNA from both input and ChIP samples was then extracted using phenol/chloroform ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein sizes were determined using the Precision Plus Protein Dual Color Standards marker (Catalog Number 161-0374) from Life Technologies.
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bioRxiv - Physiology 2021Quote: ... protein synthesis) were detected with No-Stain ™ protein labeling reagent as detailed in the manufacturer’s protocol (Invitrogen, Waltman, MA). All membranes were then blocked with 5% milk-TBST and incubated with primary and secondary antibodies as previously described [30] ...
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bioRxiv - Neuroscience 2020Quote: ... Cytokine levels were normalized to the total protein concentration in the lysate measured with BCA protein assay kit (Thermo Scientific). NO levels were analyzed using Griess Reagent Kit for Nitrite Determination (G-7921 ...
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bioRxiv - Molecular Biology 2019Quote: ... Antibody-chromatin complexes were isolated using a 1:1 mixture of magnetic Protein A- and Protein G-dynabeads (ThermoFisher Scientific) and washed three times with a solution of 50 mM HEPES-KOH ...
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bioRxiv - Biophysics 2019Quote: ... The protein was buffer exchanged 2-3x using either 7 kDa MWCO Zeba Columns for EC1-5/EC3-5 proteins or 40 kDa MWCO Zeba Columns for full length proteins (ThermoFisher).
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bioRxiv - Cell Biology 2020Quote: De novo protein synthesis was measured using Click-IT HPG Alexa Fluor 488 protein synthesis assay kit (Thermo Fisher Scientific) as per company instructions ...
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bioRxiv - Plant Biology 2021Quote: ... The protein extracts were incubated overnight with polyclonal MdDREB2A antibody and then added protein A/G agarose beads (Thermo Fisher) to incubate at 4 °C for additional 4-5 hours ...
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bioRxiv - Cell Biology 2019Quote: ... Equal amounts of proteins were loaded in SDS-PAGE gel NuPAGE™ 4-12% Bis-Tris Protein Gels (Thermo fisher) along with molecular weight marker ...
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bioRxiv - Molecular Biology 2021Quote: ... 60 μg of proteins in cell lysates were separated on a NuPAGE™ 4-12% Bis-Tris Protein Gel (Invitrogen) and transferred onto a nitrocellulose membrane (GE ...
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bioRxiv - Microbiology 2020Quote: ... Extracted proteins were separated on NuPAGE™ Novex® 4-12% Bis-Tris protein gels in MES buffer (Life Technologies) and transferred onto nitrocellulose membranes using the iBlot® system (Life Technologies) ...
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bioRxiv - Microbiology 2020Quote: ... Proteins bound to GP96 were immunoprecipitated with an antibody against Grp94 (Rat, mAb, Enzo) and Dynabeads Protein G (Thermo Scientific), then examined using mass spectrometry analysis ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 viral nucleocapsid protein (NP) was detected using the anti-NP protein antibody (Cat. # PA5-81794, Thermo Fisher) diluted 1:10000 in 0.1% tween-20/1%BSA/PBS solution as a primary antibody ...
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bioRxiv - Cancer Biology 2019Quote: ... were collected and supernatants were then incubated overnight with the following antibodies pre-incubated with Protein A and Protein G Dynabeads (Invitrogen): a mixture of AR C-terminal (441 ...
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bioRxiv - Immunology 2020Quote: ... Samples were centrifuged and the supernatants were used for total protein measurement (Pierce BCA Protein Assay, Thermo Fisher Scientific #23227) and LDH quantification (Pierce LDH Cytotoxicity Assay ...
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bioRxiv - Physiology 2019Quote: ... protein extracts were transferred to fresh tubes and protein concentrations were quantified using the PierceTM BCA assay (#23225, ThermoFisher Scientific). 25 µg of protein extract per sample was separated on 12% acrylamide gels (#5678044 ...
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bioRxiv - Molecular Biology 2019Quote: The enriched proteins were resolved by SDS-PAGE on a NuPAGE 4-12% Bis-Tris protein gel (Thermo Fisher Scientific) and stained using the Colloidal Blue Staining Kit (Life Technologies) ...
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bioRxiv - Biochemistry 2020Quote: ... The pellet was re-suspended in 5mL of 1% SDC + 50mM ABC pH 8.5 and protein concentration was measured with Pierce BCA protein assay kit (Thermo Scientific). Samples were then aliquoted into 1.5mL LoBind eppendorf tubes (Sorenson BioScience ...
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bioRxiv - Microbiology 2019Quote: ... The suspension was centrifuged at 12,000 g for 5 min and the protein supernatant was quantified using the BCA protein assay kit (ThermoFisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Immuno-precipitation was done overnight at 4°C using Protein A-coupled or Protein G-coupled Dynabeads previously incubated with anti-GFP A11122 antibody (Invitrogen), anti-Flag antibody (M2 Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... ATP concentrations were normalized to total protein content as determined by a Micro BCA protein assay kit (ThermoFisher, Cat # 23235). ATP was measured in technical triplicates ...
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bioRxiv - Neuroscience 2019Quote: ... Protein quantitation was performed using Ionic Detergent Compatibility Reagent for Pierce™ 660nm Protein Assay Reagent (ThermoFisher Scientific, Waltam, MA) or BCA method ...
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bioRxiv - Physiology 2021Quote: ... Supernatant (cleared lysates) were used to determine total protein amount using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... Supernatant (cleared lysates) were used to determine total protein amount using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... Supernatant (cleared lysates) were used to determine total protein amount using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... and the protein concentration determined for normalization purposes using a Pierce BCA protein assay kit (Thermo Scientific, Waltham, MA, USA). The remainder of the sample was heated to 95°C for 10 min ...
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bioRxiv - Biochemistry 2021Quote: ... The remaining soluble protein fraction was collected and quantified for protein concentration via bicinchoninic acid (BCA) assay (Pierce Thermo Fisher). The soluble protein concentration was then used as an indication of lysis efficiency/cell mass to determine the amount of 8 M urea (dissolved in PBS ...
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bioRxiv - Genomics 2021Quote: ... The chromatin fragments bound to antibodies (after incubation overnight) were captured using 40 μl of protein-A or protein-G Dynabeads (Invitrogen; pre-washed with 0.1% BSA [Fraction V ...
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bioRxiv - Biophysics 2021Quote: ... The SNAP-tag allows for covalent binding of O6-benzylguanine (BG) to the protein.[63] Protein expression was performed in Escherichia coli BL21(DE3) pRARE (Invitrogen) under T7 promoter with 1 mM isopropyl-β-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Cell Biology 2020Quote: ... Equal loading was ensured by conducting protein concentration measurements with the Pierce BCA Protein Assay Kit (Thermo Scientific, cat# 23225). After SDS-PAGE and western blot transfer ...
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bioRxiv - Microbiology 2021Quote: ... cytoplasmic proteins and the total cell lysate proteins were denatured and boiled with 4× LDS sample buffer (Cat. #NP0007, Invitrogen) and subjected to immunoblotting analysis with equal protein loading of extracts (~20 µg/lane) ...