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Citations for Thermo Fisher :
5651 - 5700 of 10000+ citations for Zika Virus NS1 Protein Uganda strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The supernatant of lysates was assessed for protein content using the Coomassie Protein Assay Reagent (23200, Thermo Fisher Scientific) and spin filtered through Amicon Ultra-0.5 centrifugal filter units (10 kDa MWCO ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were collected and protein concentration was determined using the Pierce Rapid Gold BCA Protein Assay Kit (Thermo Scientific). Equal concentrations of protein extract were loaded into 4%–12% NuPAGE BisTris gels (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Protein quantification of the collected exosomes was performed using BCA method (Pierce™ BCA Protein Assay kit, ThermoFisher Scientific) and equal amount of exosomes were collected for downstream applications ...
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bioRxiv - Cell Biology 2022Quote: ... The MV protein fractions were detected on the protein gel using Simple-Blue™ Safe Stain (ThermoFisher Scientific, UK). The sandwich method was then used to transfer the proteins from the gel to a methanol-activated polyvinylidene fluoride (PVDF ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatant was transferred to a new tube and protein concentrations were determined using Pierce BCA protein assay (ThermoFisher, 23225). Samples were stored at -80 °C until further use ...
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bioRxiv - Neuroscience 2020Quote: ... Supernatant was kept and protein concentration was measured using the BCA method (Pierce BCA Protein Assay, Thermo Fisher, #23225). Samples were incubated in 2x Laemmli Buffer (120mM Tris-HCl ...
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bioRxiv - Immunology 2020Quote: ... The total protein concentration in each cell lysate was measured using Pierce BCA Protein Assay Kit (Thermofisher, Waltham, MA). The cell lysates containing approximately 20 µg of protein were resolved on Novex™ NuPAGE™ 4-12% Bis-Tris Protein Gels (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Protein content of whole cell lysate was measured with the BCA protein assay (Pierce™, Thermo Scientific, Landsmeer, Netherlands) to quantify the protein content of the samples in order to equalize for input material for SDS page ...
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bioRxiv - Neuroscience 2019Quote: ... 10 μl/mg) and protein content was measured by the Pierce™ Bicinchoninic Acid protein assay (BCA; Thermofisher Scientific). 5 μl (4 μg protein/μl ...
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bioRxiv - Neuroscience 2019Quote: ... Myc-tagged proteins were immunoprecipitated using anti-c-Myc monoclonal antibody supernatant (9E10, DSHB) and protein A-agarose (Invitrogen). Flag-tagged proteins were immunoprecipitated using anti-Flag-M2 affinity gel (Sigma) ...
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bioRxiv - Pathology 2021Quote: ... The protein concentrations of homogenized tissues were determined using Pierce™ BCA Protein Assay Kit (Thermo Fisher, Cat# 23225). The homogenized tissues were then incubated with CM-H2DCFDA probe at 37 °C for 20 min with protection from light ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... The protein concentration of cleared samples was determined with a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, #23225) with measurements conducted on a GloMax-Multi Plate Reader (Promega ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein was recovered by centrifuge and quantitated by Pierce™ BCA Protein Assay Kit (23227, Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... according to manufacturer’s instructions and protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Protein lysates were subjected to SDS-PAGE and Western blot.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein concentration was measured using the Pierce™ BCA® Protein Assay Kits (Thermo Fisher Scientific Inc., cat.no. 23227). CD63 antibody (1:500 Thermo Fisher Scientific Inc. ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were normalized to equal protein concentrations (0.333 mg/ml) using Pierce BCA protein assay (Thermo Fisher Scientific, USA).
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bioRxiv - Microbiology 2021Quote: ... The supernatant was collected and total protein concentration was measured using the Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Biochemistry 2021Quote: ... The total protein concentration of the supernatants was determined by the Pierce bicinchoninic acid (BCA) protein assay (Thermo Scientific). For the pulldown experiments ...
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bioRxiv - Cell Biology 2021Quote: ... Equal amount of protein was used from each sample as determined by BCA protein Assay (ThermoFisher scientific, Cat; 23235).
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bioRxiv - Cell Biology 2020Quote: ... The total extravesicular protein content was quantified using the micro bicinchonic acid protein assay (BCA) (#23235, Thermo Fisher scientific).
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bioRxiv - Bioengineering 2021Quote: ... Protein concentration was determined using Pierce™ BCA (Bicinchoninic Acid) Protein Assay Kit (Thermo Fisher Scientific Inc. Waltham, MA) following manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: ... and the total protein content was determined photometrically with the Pierce bicinchoninic acid (BCA) Protein Assay Kit (Thermo Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... aliquots of the supernatant were subjected to protein quantification with a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) and SDS-PAGE using a TGX Stain-Free FastCast Acrylamide Kit (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... 2010) as follows: protein samples were separated in NuPAGE™ 3-8% Tris-Acetate Protein Gels (Thermo Fisher EA03755) in Tris-Acetate-EDTA running buffer (Thermo Fisher BP13354 ...
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bioRxiv - Microbiology 2022Quote: ... Protein concentrations of the purification fractions were estimated using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific) according to the manufacturers protocol.
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bioRxiv - Systems Biology 2022Quote: ... The protein concentration of the samples was measured using a Pierce BCA Protein Assay Kit (Thermo Scientific, Cat#23225). To determine the proteasome activity ...
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bioRxiv - Developmental Biology 2022Quote: ... 20μg of each respective sample was loaded onto a 1mm 3-8% Tris-Acetate Mini Protein gel (NuPAGE) along with 10μl of Spectra Multicolor High Range protein ladder (Thermofisher). The gel was run at 80V for 15 minutes and then increased to 120V until the dye front reached the bottom ...
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bioRxiv - Cancer Biology 2022Quote: ... and normalized to protein concentration measurement carried out using Pierce™ BCA Protein Assay Kit (Thermo Scientific, Cat. 23227).
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bioRxiv - Developmental Biology 2022Quote: ... The whole sperm protein extract thus obtained was quantified using Pierce™ 660nm protein assay reagent (Thermo Scientific™) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2019Quote: ... 50µg of protein sample along with 5µl of Page RulerTM pre-stained protein ladder (Cat no. 26617, Thermo Scientific), was loaded and gel was run initially at 50 volts until the tracking dye crosses the stacking gel ...
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bioRxiv - Microbiology 2019Quote: ... The total protein content in the infected cells was determined using a BCA protein assay kit (Thermo Scientific, USA) with bovine serum albumin as a standard ...
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bioRxiv - Genetics 2019Quote: ... Biotinylated surface proteins were recovered by incubating 200 μg of total protein with streptavidin-agarose beads (20347, Thermo Fisher) and eluting in Laemmli sample buffer ...
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bioRxiv - Physiology 2020Quote: ... The supernatant was saved and protein was quantified using a Pierce BCA protein assay kit (ThermoFisher, catalog number 23225). 100 ug of protein was used per sample ...
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bioRxiv - Microbiology 2020Quote: ... The supernate was collected and protein was quantified by BCA assay (Pierce BCA Protein Assay Kit ThermoFisher Scientific, 23225). To measure ACE2 expression in transfected cells ...
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bioRxiv - Neuroscience 2019Quote: ... Membrane proteins were extracted using the Mem-PER Plus Membrane Protein Extraction Kit (Cat# 89842; ThermoFisher Scientific, Waltham, MA) according to the manufacturer’s instructions using a mixture of protease and phosphatase inhibitors (10 µl/ml ...
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bioRxiv - Physiology 2019Quote: ... 15 μl of homogenate was used for protein measurement using a Pierce BCA protein Assay Kit (#10678484, Fisher Scientific) and a standard curve was calculated with a series of Albumin dilutions (0.025 ...
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bioRxiv - Molecular Biology 2020Quote: ... The ladder used for proteins larger than 240 kDa was 12 µl HiMark Pre-Stained Protein Standard (Thermo Scientific). The ladder used for proteins smaller than 240 kDa was 2.5 µl BLUltra Prestained Protein Ladder (GeneDireX) ...
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bioRxiv - Molecular Biology 2020Quote: ... CSDE1 protein was then captured by incubation with an affinity purified CSDE1 antibody conjugated to protein A Dynabeads (Invitrogen) for 4 hours at 4°C with gentle rotation ...
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bioRxiv - Microbiology 2020Quote: ... Ten μg of protein per sample was loaded on NovexTM 10-20% Tris-Glycine protein gels (Thermo Fisher Scientific) and transferred onto iBlot2 Transfer Stacks PVDF membrane (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: Protein concentration of each qEV fraction was estimated by BCA protein assay according to the manufacturer’s protocol (ThermoFisher Scientific). Technical replicates by serial dilution were performed.
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bioRxiv - Pharmacology and Toxicology 2021Quote: Total BAL protein was quantified with the Qubit Total Protein Quantification kit and the Qubit 2.0 fluorometer (Thermo Scientific). BAL cytokines were profiled using a pre-mixed MILLIPLEX protein immunoassay (Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... Total protein concentrations of the cell lysates were determined with a BCA protein assay kit (Thermo Fisher Scientific, USA). The cell lysates were pre-cleaned by an off-target control antibody and aliquoted ...
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bioRxiv - Neuroscience 2019Quote: ... Protein content was quantified using the Pierce™ BCA Protein Assay kit according to manufacturer protocols (Thermo Fisher Scientific). 20 μg of protein lysate was run on 4-20% Mini-PROTEAN TGX Precast Gel (BioRad) ...
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bioRxiv - Developmental Biology 2019Quote: Protein concentrations for individual samples were determined using a BCA protein assay following the manufacturer’s protocol (Thermo Scientific Pierce). 50 μg of protein were labeled with Alexa Fluor 555 and all reactions were quenched using 2M Tris (pH 6.8 ...
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bioRxiv - Synthetic Biology 2020Quote: Protein quantification was performed using the protocol and supplies provided by the BCA Protein Assay Kit (Thermo Scientific Pierce). Samples of BSA protein in PBS ranging in concentration from 25 ug/ml to 2000 ug/ml were used to create the standard concentration curve ...
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bioRxiv - Genetics 2020Quote: ... the supernatant was used to assess protein concentration using the Pierce BCA Protein Assay kit (Thermo Fisher Scientific, #23225) before subsequent analysis ...
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bioRxiv - Genomics 2021Quote: ... The protein concentration of supernatant lysates was determined using the Pierce 660 nm Protein Assay Kit (Thermo Fisher Scientific) and a NanoDrop1000 Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Supernatants from each filter were transferred into eppendorfs and protein concentrations were determined using Pierce Protein Quantitation Assay (ThermoFisher). Protein concentrations across samples were normalized to 4 mg/ml using 50mM Tris (pH 7.5) ...
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bioRxiv - Biochemistry 2019Quote: ... 2 µL were used to measure protein concentration by a BCA protein assay kit (Thermo Fisher Scientific, Roskilde, Denmark).
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bioRxiv - Systems Biology 2019Quote: ... The protein concentration in the cell lysate was quantified with a Pierce BCA Protein Assay Kit (ThermoFisher Scientific, UK) using the provided albumin as standard ...