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Citations for Thermo Fisher :
6101 - 6150 of 10000+ citations for Zika Virus NS1 Protein Uganda strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... transfected cell lysates (500 µg total protein in 200 µl) were pre-cleared by incubating with 10 µl Dynabeads protein G (Invitrogen) at 4°C for 1 hr ...
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bioRxiv - Immunology 2023Quote: ... 2% SDS and 10% Glycerol) and protein concentration was measured using Pierce™ BCA Protein Assay kit (Thermo-Fisher Scientific). 20µg of protein was resuspended with SDS-loading dye (50% β-Mercaptoethanol and 0.02% Bromphenolblue) ...
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bioRxiv - Developmental Biology 2023Quote: Membrane proteins were extracted from cell pellets using the Mem-PER™ Plus Membrane Protein Extraction Kit (89842, Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... and 10-15 µg of protein were loaded on 4-12% Bis-Tris Midi NuPage Protein Gels (Thermo Fisher Scientific) and separated at 120V for 2 h in MES running buffer ...
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bioRxiv - Neuroscience 2023Quote: ... 14.000 RPM) and protein concentration of collected supernatants measured using the Pierce BCA Protein Assay Kit (#323227, Thermo Scientific, 23227). The samples adjusted to contain equal amounts of protein (10 μg or 12 μg ...
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bioRxiv - Physiology 2024Quote: ... Supernatant (cleared lysates) were used to determine total protein amount using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The protein content of the samples was quantified using the Pierce™ 660 nm protein assay kit (Thermo Fisher, US) with BSA as the standard ...
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bioRxiv - Neuroscience 2023Quote: The protein concentration of the cell lysates was determined with the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). For co-immunoprecipitation ...
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bioRxiv - Cancer Biology 2023Quote: ... One mg of total protein/sample were incubated for 6 h at 4°C with protein A/G-magnetic beads (Dynabeads, Invitrogen) prebound with 5 μg of anti-mCherry antibody ...
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bioRxiv - Biophysics 2023Quote: ... The final protein concentration was measured with the Micro BCA Protein Assay Kit (#OI191202, Thermo Fisher Scientific, Waltham, MA, USA). Protein purity was verified by SDS-PAGE and silver staining (Fig ...
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bioRxiv - Biophysics 2023Quote: ... the total protein concentration was determined using the bicinchoninic acid (BCA) protein assay kit (Pierce/Thermo Fisher Scientific; Rockford, IL). 25µg of protein samples were mixed with 4x LDS sample buffer (Life Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... Lysate inputs were normalized by determining overall protein concentration using Pierce™ BCA Protein Assay (Thermo Fisher Scientific, Waltham, MA). Subsequently ...
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bioRxiv - Cancer Biology 2023Quote: ... One mg of total protein/sample were incubated for 6 h at 4°C with protein A/G-magnetic beads (Dynabeads, Invitrogen) prebound with 5 μg of the corresponding antibody ...
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bioRxiv - Cell Biology 2023Quote: Protein size separation of 25 µg of protein was done on a NuPAGE 4-12% Bis-Tris gel (Invitrogen, NP0336BOX) in NuPAGE MES SDS-buffer (Invitrogen ...
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bioRxiv - Systems Biology 2023Quote: We quantified Protein synthesis rate with the “Click-iT HPG Alexa Fluor 488 Protein Synthesis Assay Kit” (Thermo Fisher Scientific), with minor adaptations from the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Nascent protein synthesis was measured with the Click-iT HPG Alexa Fluor 488 Protein Synthesis Assay Kit (cat. no. C10428, ThermoFisher). Cells were plated in 12-well plates (Cell Treat ...
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bioRxiv - Evolutionary Biology 2023Quote: ... protein concentration of the CF was determined using the Coomassie (Bradford) Protein assay Kit (Thermo Fisher Scientific, Rockford, IL, USA) and bovine serum albumin (BSA ...
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bioRxiv - Evolutionary Biology 2023Quote: ... protein concentration in the supernatant was determined using the Coomassie (Bradford) Protein assay Kit (Thermo Fisher Scientific, Rockford, IL, USA) and bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatant proteins were then quantified using the BCA assay and equivalent protein concentrations (800 µg) were immunoprecipitated with DynabeadsTM Protein G (Invitrogen) coupled to a primary antibody at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were quantified via a BCA assay using the Pierce BCA Protein Assay Kit obtained from Life Technologies (Catalog #23225). Cell lysates were run through a 4-12% SDS-PAGE gel electrophoresis at 140-170V ...
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bioRxiv - Bioengineering 2023Quote: Concentrated EV or VLP total protein concentrations were determined using the Pierce BCA protein assay kit (Thermo Fisher Scientific, USA) These neutralization reagents were mixed with 4X loading dye ...
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bioRxiv - Cancer Biology 2024Quote: ... The insoluble fraction was removed via centrifugation (13,000xg, 10 min, 4 °C) and protein abundance was quantified by BCA Protein Assay (Thermo Scientific). 50 μg of protein was utilized per well in a total volume of 60 μL ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5 M sucrose) and a protein concentration assay was performed (BCA Protein Assay Kit, Pierce-Thermo Fisher Scientific, Melbourne, Australia). 100 μg of isolated mitochondria were collected and centrifuged at 12,000 g for 15 min at 4 °C and after removal of the supernatant the pellet was frozen at −80 °C for subsequent proteomics analysis.
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bioRxiv - Developmental Biology 2023Quote: ... Protein pellets were resuspended in 1% SDS and protein concentration was measured using the Bradford assay kit (Thermo Scientific, 1863028), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Protein concentration was measured with Pierce™ BCA Protein Assay Kit according to manufacturer’s guidelines (Thermo Fisher; Cat. No. 23225). Protein samples were mixed with 4X NuPAGE™ LDS Sample Buffer (Thermo Fisher ...
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bioRxiv - Physiology 2024Quote: ... The total islet protein content for each secretion assay was measured with Micro BCA™ Protein Assay Kit (ThermoFisher, #23235). Data represent the glucagon or insulin content per µg of protein in each tube.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and the protein concentration in the supernatant was determined using a BCA Protein Assay Kit (Cat# 23225, Thermo Fisher Scientific). Afterward ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were transferred to new tubes and protein concentrations were determined using the BCA Protein Assay Kit (Thermo Fisher Scientific). Between 20-50 μg of protein per sample was mixed with Laemmli sample buffer and boiled for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... The supernatants (1.2 mL) were subjected to immunoprecipitation with 30 μG of protein G magnetic beads (Dynabeads Protein G, Thermo Scientific) overnight ...
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bioRxiv - Cell Biology 2024Quote: ... The 20 μg of extracted proteins were quantified using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, WA, USA) at 500 ng/μL ...
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bioRxiv - Neuroscience 2024Quote: ... prior to proceeding with protein quantification using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, cat. no. 23225). 10µg of proteins were resolved using SDS-PAGE (4-12% Bis-Tris precast gels in buffer MES) ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration in the tissue lysates were determined by Pierce™ BCA protein assay kit (Thermo Scientific, Cat# 23227, USA). Protein lysates were suspended in sample buffer containing NuPAGE™ LDS Sample Buffer (Invitrogen™ ...
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bioRxiv - Neuroscience 2024Quote: Protein samples prepared from heads of 10-20 males and females in protein extraction buffer plus 1% protease inhibitor (ThermoFisher) and 1% phosphatase inhibitor (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... with 2 µl of supernatant per sample used to test protein concentration (Pierce 660 nm Protein Assay, Invitrogen, Cat: 22662). Samples were stored in a -20°C freezer prior to LC/MS-MS ...
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bioRxiv - Immunology 2024Quote: ... 0.3 µg of each gRNA was mixed with 2 µg Cas9 protein (TrueCut™ Cas9 Protein v2, Thermo Fisher Scientific) in 5 µl E8 medium supplemented with 10 μM Y27632 ...
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bioRxiv - Cell Biology 2024Quote: ... Eluates were mixed with protein sample buffer for SDS-PAGE and analyzed with SYPRO Ruby protein gel stain (ThermoFisher S12000), autoradiography or western blotting.
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bioRxiv - Microbiology 2024Quote: ... The total protein content in the supernatant fraction was determined by Pierce™ BCA protein assay kit (Thermo Fisher Scientific). An equivalent amount of total proteins was loaded in each lane and resolved on a 15% SDS-PAGE gel or 4-12% SurePAGE™ Bis-Tris gel (Genscript) ...
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bioRxiv - Systems Biology 2024Quote: ... Lysates were centrifuged for 10 min at 14.000 rpm and 4°C followed by protein determination with PierceTM BCA Protein Assay Kit (Thermo Scientific) according to manufacture description ...
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bioRxiv - Immunology 2024Quote: Purified recombinant protein vaccines were analyzed on SDS-PAGE by staining with GelCode Blue Safe Protein Stain (Thermo Fisher Scientific). Proteins separated by SDS-PAGE were also transferred onto nitrocellulose membranes for immunoblotting ...
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bioRxiv - Microbiology 2024Quote: The protein concentration of gradient fractions 6 and 17 was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher). Ten micrograms of the purified fractions 6 (exosome fraction ...
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bioRxiv - Neuroscience 2024Quote: Total protein levels were quantified in tissue homogenates in triplicates using the Pierce Microplate BCA Protein Assay Kit (Thermo Scientific Pierce Protein Biology ...
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bioRxiv - Neuroscience 2024Quote: ... protein concentrations were quantified using a Bicinchoninic acid assay (BCA) with the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). The lysates were diluted to a concentration of 1.5 μg/μL and stored at -80°C until analyzed by Western blot.
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bioRxiv - Molecular Biology 2024Quote: ... Supernatants were transferred to new tubes and protein concentrations were determined by BCA Protein Assay Kit (Thermo Fisher Scientific, 23225). Between 20-50 μg of protein per sample was mixed with Laemmli sample buffer (62.5 mM Tris-HCl (pH 6.8) ...
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bioRxiv - Plant Biology 2020Quote: ... Dynabeads Protein A or G (Thermo Fisher Scientific) were used ...
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bioRxiv - Plant Biology 2020Quote: ... 20 μl of slurry Protein G beads (Invitrogen) per gram of tissue were added and subjected to a further incubation for 2 hours with rotation at 4°C ...
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bioRxiv - Plant Biology 2019Quote: ... were used with Dynabeads Protein G (Thermo Scientific). Protein– DNA complexes were eluted from the beads as described in (Rowley ...
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bioRxiv - Genetics 2021Quote: ... Pierce™ BCA protein assay kit (Thermo Fisher) was used to quantify the protein levels in the supernatant ...
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bioRxiv - Biochemistry 2021Quote: ... Expi293F cells used for protein expression (ThermoFisher Scientific) were cultured in Expi293 media (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... and Dynabeads Protein G (ThermoFisher SCIENTIFIC, Cat #10004D). RNA was partially digested with RNase I (ThermoFisher SCIENTIFIC ...
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bioRxiv - Cancer Biology 2021Quote: ... Coomassie Protein Assay Reagent kit (Thermo Fisher Scientific) was used to measure protein concentration ...