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Citations for Thermo Fisher :
5801 - 5850 of 10000+ citations for 7 Chloromethyl 2 2 dimethyl 2 3 dihydro 1 benzofuran since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines were cultured with the 2:1 mixture of IMDM (Life technologies) and Ham’s-F12 nutrient mixture (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... Primary antibodies include Anti-beta tubulin (1:500, mouse monoclonal, 2 28 33, Invitrogen) and Anti-Hec1 (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... HET(1) and HET(2) hESCs was extracted using mirVana microRNA isolation kit (ThermoFisher). Small RNA libraries were generated using NEXTFlex Small RNA Library Prep Kit v3 (Cat #NOVA-5132–06 ...
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bioRxiv - Biochemistry 2024Quote: ... containing 2% sodium dodecyl sulfate and 1 mM PMSF protease inhibitor (Thermo Fisher Scientific). Following a 30-minute incubation on ice with periodic vortexing ...
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bioRxiv - Microbiology 2024Quote: ... as well as DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride) (1:1000; Invitrogen, #D1306) were diluted in DPBS with 1% FBS and incubated for 2 h at 4 °C ...
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bioRxiv - Systems Biology 2024Quote: ... A 1:5000 solution of DAPI (4’,6-diamidino-2-phenylindole) (Thermo Fisher, 62248) diluted blocking buffer was added for 15 mins at room temperature to stain for DNA ...
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bioRxiv - Biophysics 2024Quote: ... transfected 2 d later with 1 µg/well plasmid DNA using Lipofectamin LTX (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... The blood was diluted 1:2 with sterile 1X D-PBS (Gibco, 14190-094), and then slowly added on top of Histopaque-1077 ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 mM L-glutamine and 1× non-essential amino acids (all reagents from Gibco). KrasRR expression was induced in vitro by adenovirus-mediated recombination ...
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bioRxiv - Cell Biology 2024Quote: ... Nuclei were stained with 4′-6-diamidino-2-phenylindole (DAPI) (1 µg/mL, Invitrogen), and actin with Alexa FluorTM 647/546-labelled phalloidin (1:400 ...
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bioRxiv - Cancer Biology 2020Quote: ... CellEvent Caspase-3/7 Detection Reagent (Invitrogen) and Hoescht were added to the media at concentrations listed in S4 Table and incubated for 30min at 37°C in 5% CO2 ...
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bioRxiv - Cell Biology 2020Quote: ... CellEvent Caspase-3/7 (Life Technologies C10723), Fis1 polyclonal antibody (Proteintech 109561-AP) ...
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bioRxiv - Cell Biology 2024Quote: ... and caspase 3/7 (Thermo Fisher Scientific) were prepared following the manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Caspase 3/7 (Thermo Fisher Scientific, #C10423) activation and propidium iodide (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... samples were blocked for 1 hour in 2 mg ml−1 BSA and 1% Goat Serum (Thermo Fisher) in 1X PBSTXD (1X PBS ...
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bioRxiv - Immunology 2024Quote: ... CD206-FITC (19.2, 1:100), CD11c-PE (3.9, 1:50) and CD14-PerCP-Cy5.5 (61D3, 1:200) from eBiosciences (ThermoFisher) were used.
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bioRxiv - Cell Biology 2022Quote: ... The medium was replaced at day 1 to remove non-adherent cells and afterwards every 2-3 day with DMEM/10% FBS/1% penicillin streptomycin (PS) (Gibco, USA, catalog number 15140-122). These cells were passaged serially when cells reached to 80-100 % confluency or when proliferation essentially ceased by using 0.25% Trypsin/EDTA (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... Patient-derived primary cell line GCDX62 was maintained in a 3:1 mixture of Keratinocyte-SFM (KSF; Thermo Fisher Scientific; supplemented with 2% (v/v) FCS ...
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bioRxiv - Immunology 2023Quote: ... Purified T cells were activated for 1 to 3 days with Human T-Activator anti-CD3/CD28 dynabeads at a 1:2 bead-to-cell ratio (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). Prior to analyses ...
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bioRxiv - Plant Biology 2024Quote: ... 1cm long root tip sections of four plants were pooled and rolled up in a 1 x 3 cm strip of pre-moistened tissue paper and placed into 2 mL Screw Glass Vials (Thermo Scientific, Cat. No. 6PSV9-1P) filled with 200 μL of water ...
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bioRxiv - Immunology 2020Quote: ... Detection of active caspase 3/7 was accomplished using CellEvent™ Caspase-3/7 Red Detection Reagent (ThermoFisher Scientific). Sorted CD4SP Rag1GFP+ thymocytes were stained with CD5-PerCP-Cy5.5 (53-7.3 ...
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bioRxiv - Cell Biology 2020Quote: ... 2ml serum free DMEM or 2) 2 mL Cell Mask Orange (CMO: 2.5 µg/mL in serum free DMEM) (Thermofisher Scientific cat C10045) and both were incubated at 37° C for 10 minutes.
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bioRxiv - Neuroscience 2020Quote: ... Primary human dermal fibroblasts were collected from VWMD patients (2) or control family members (2) and maintained in DMEM/F12 (Life Technologies, 21331020), 10% FBS (Bovogen ...
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bioRxiv - Synthetic Biology 2021Quote: ... Supernatant was harvested at the indicated timepoints and IL-2 levels in the supernatant were measured via IL-2 Human Instant ELISA kit (Thermo Fisher #BMS221INST). T-cell proliferation was also measured at the indicated timepoints using a BD FACSymphony Fortessa X-50.
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bioRxiv - Cell Biology 2021Quote: ... cells were pulsed with 10µM 5-Ethynyl-2’deoxyuridine (EdU) for 2 hours following manufacturer’s instructions (Click-iT® EdU Alexa Fluor® Imaging Kit, Life Technologies). Cells were subsequently fixed and stained with Hoechst 33342 ...
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bioRxiv - Neuroscience 2022Quote: ... 2.8×106 neural progenitor cells were grown on glass coverslips in 6-well plate and loaded with 2 µM Fura-2/AM and Pluronic F127 in OptiMEM (Thermo Fisher Scientific) for 30 min at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: 3 μl of purified ribosome sample was applied to Quantifoil 300 mesh R2/2 grids with an ultrathin 2 nm carbon layer and frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) at 15 °C ...
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bioRxiv - Biophysics 2022Quote: ... The sample was diluted to 0.5 mg/ml and directly applied to freshly glow-discharged 2/2 Cu Quantifoil grids in a Vitrobot Mark IV (Thermo Fisher Scientific) at 4° C and 100% humidity ...
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bioRxiv - Microbiology 2020Quote: ... Tryptic peptides (2 μg) were loaded onto a C18 trap (75 um x 2 cm; Acclaim PepMap 100, P/N 164946; Thermo Fisher Scientific) at a flow rate of 2 ul/min of solvent A (0.1% FA and 3% acetonitrile in LC-MS grade water) ...
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bioRxiv - Microbiology 2021Quote: ... incubated in primary antibody (anti-SARS-CoV-2 Spike, which detects the S2 subunit of SARS-CoV-2 S (Invitrogen, PA1-41165), anti-GAPDH (proteintech ...
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bioRxiv - Neuroscience 2020Quote: ... sequences were amplified from plasmids derived from ser-2(2)p::FRT::YFP,andclonedupstreamof FRT::STOP::FRT::GCaMP6s to generate WY016 using Gateway (Thermo Fisher Scientific). To generate PSAB1206 ...
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bioRxiv - Cancer Biology 2022Quote: ... was diluted in T cell medium (RPMI 1640 + 10%FBS + L-glutamine (2 mM) + penicillin (100 U/ml + streptomycin (100 μg/ml) + 55 μM 2-Mercaptoethanol (Thermo Fisher, #21985023)) ...
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bioRxiv - Microbiology 2021Quote: ... incubated in primary antibody (anti-SARS-CoV-2 Spike, which detects the S2 subunit of SARS-CoV-2 S (Invitrogen, PA1-41165), anti-GAPDH (proteintech ...
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bioRxiv - Cell Biology 2021Quote: Amnion and choriodecidua explants (2 cm2) were placed in six-well plates with 2 ml of DMEM/F12 (Gibco, Thermo Fisher Scientific) supplemented with 10 % fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: Amnion and choriodecidua explants (2 cm2) were placed in six-well plates with 2 ml of DMEM/F12 (Gibco, Thermo Fisher Scientific) supplemented with 10 % fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... scrambled or PUS1/PUS7ko HEK-293Ts were transfected with 2 μg PAN and 2 μg ORF57-eGFP plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and rested for 30 minutes at room temperature in a buffer consisting of 2% FBS in 1X PBS (2% FACS buffer) with 50nM dasatinib (Thermo Fisher Scientific). Cells were washed with 2% FACS buffer with 50nM dasatinib and incubated with the Gag386-394GW9 tetramer for 45 minutes at room temperature ...
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Plastid double-strand RNA transgenes trigger small RNA-based gene silencing of nuclear-encoded genesbioRxiv - Plant Biology 2022Quote: ... The gel was imaged and washed 2 x 15 minutes in nuclease-free water then 2 x 15 minutes in 10X UltraPure SSC Buffer (Invitrogen, Carlsbad, CA) with gentle shaking ...
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bioRxiv - Molecular Biology 2023Quote: ... HeLa (CRM-CCL-2) and U-2 OS (HTB-96) cells obtained from ATCC were cultured in DMEM (Thermo Fisher Scientific, 12800017) supplemented with 10% fetal bovine serum (Lonsera ...
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bioRxiv - Biochemistry 2023Quote: ... To initiate reaction, 200 µL of Assay Buffer with substrate mixture (30 mM K3PO4, 2 mM MgSO4, 2 mM DTT (Fisher Scientific, AAJ1539706), 0.56 mM TPP (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... 25 mM Tris pH 7.4, 2 mM EDTA, 2 mM EGTA, 100 μM PR-619 (Selleckhem, cat. # 7130) and protease inhibitors cocktail (Thermo Scientific, cat. # 784115,) by vortexing ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were transferred to a nitrocellulose membrane (iBlot 2 Transfer Stacks, mini) in an iBlot 2 Gel Transfer Device (Invitrogen; Carlsbad, CA). Immunodetection was carried out using commercially available primary antibodies against PCCA (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... the disulfide bond linking the two Fab fragments of F(ab’)2 goat anti-mouse Ig(G+M) was reduced using 2-mercaptoethylamine HCL (Thermo Fisher Scientific) and biotinylated by using maleimide-PEG2-biotin (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... where (+T) is LNA] was diluted to a final concentration of 2 ng/µl in hybridization buffer [2× SSC (Thermo Fisher Scientific), 0.1 mg/ml yeast tRNA (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... and 12 old (21 months) male C57BL/6 animals (combined over 2 independent experiments) were intraperitoneally injected with 5-ethynyl-2’- deoxyuridine (EdU) (Fisher Scientific, A10044) (resuspended in PBS at 5 mg/mL ...
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bioRxiv - Developmental Biology 2022Quote: ... embryos at the desired developmental stage were fixed for one hour using MAD fixative (2 parts methanol [Thermo Fisher Scientific, Waltham, MA], 2 parts acetone [Thermo Fisher Scientific,
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bioRxiv - Biochemistry 2024Quote: ... Peptide separation was performed on an EASY-Spray C18 reversed-phase nano-LC column (Acclaim PepMap RSLC; length - 50 cm; inner diameter - 2 μm; particle size - 2 μm; pore size – 100 Å; Thermo Scientific) at 55 °C and a flow rate of 300 nL/min ...
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bioRxiv - Genomics 2023Quote: ... plugs were melted for 2 minutes at 70°C and solubilized with 2 μL of 0.5 U/μL AGARase enzyme (ThermoFisher Scientific, Waltham, MA) for 45 minutes at 43°C ...
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bioRxiv - Neuroscience 2024Quote: ... Digested ganglia were gently washed once in ‘basal medium’ and twice in ‘complete medium’ (Neurobasal A medium supplemented with 2% B27 [Invitrogen/Thermo Fisher], 2 ng/ml GDNF [Invitrogen/Thermo Fisher] and 10 ng/ml NT4 [PeproTech ...
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bioRxiv - Microbiology 2023Quote: ... the cultures were co-transfected with 2 µg of pCHMP-NS3-Flag and 2 µg of pGag using 4 µL of 293Fectin Reagent (Thermo Fisher Scientific). Cells were pelleted and high-pressure frozen as described in [68] using an HPM100 system (Leica Microsystems) ...