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Citations for Thermo Fisher :
5601 - 5650 of 10000+ citations for 7 Chloromethyl 2 2 dimethyl 2 3 dihydro 1 benzofuran since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... and cell viability was then determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell viability assay (Invitrogen, USA) according to the manufacturer’s protocol 28 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The reaction solution was vortexed for 2-3 seconds to avoid bubbles and added to 384-well plate (Nunc™ MicroWell™ 384-Well Optical-Bottom Plates ...
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bioRxiv - Cell Biology 2020Quote: ... 2×106 NP-TTD were transfected with 3 µg plasmid via electroporation using Neon™ Transfection System (MPK1096, Invitrogen) with following parameters ...
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bioRxiv - Molecular Biology 2022Quote: Fresh tissue samples were washed 2–3 times in PBS and incubated in 5 U/ml dispase (ThermoFisher Scientific) supplemented with antibiotics (penicillin 50U/I and streptomycin 50 mg/ml ...
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bioRxiv - Biochemistry 2022Quote: ... 4,4-difluoro-5-(2-thienyl)-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid (BODIPY-C12, Invitrogen D3835), was dissolved in 100% ethanol and conjugated to 10% bovine serum albumin ...
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bioRxiv - Neuroscience 2023Quote: Cellular viability was determined utilizing a MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay (Invitrogen, Waltham MA), as described previously (Kumar et al. ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 3’ end RNA was synthesized by in vitro transcription using an mMESSAGE mMACHINE T7 kit (Invitrogen) with a DNA template (5’- TAATACGACTCACTATAGCTATCCCCATGTGATTTTAATAGCTTCTTAGGAGAAUGACGTAGCATGCTACGCG -3’ ...
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bioRxiv - Neuroscience 2022Quote: ... We reverse-transcribed ∼2-3 µg DNase treated RNA into cDNA using Superscript III Reverse Transcriptase (Invitrogen no. 18080093). For each reaction ...
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bioRxiv - Cell Biology 2024Quote: ... equipped with a trapping column (3 µm C18 particle, 2 cm length, 75 µm ID, Acclaim PepMap, Thermo Scientific) and a 50 cm long separation column (2 µm C18 particle ...
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bioRxiv - Molecular Biology 2023Quote: ... coupled to an Acclaim PepMap 100 column (2 cm x 75 μm, 3 μm particle size, Thermo Fisher Scientific) coupled in line with an EASY-Spray PepMap RSLC C18 reversed-phase column (50 cm x 75 μm ...
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bioRxiv - Microbiology 2023Quote: ... tilt series were acquired at binning 2 on a Falcon 3 camera under the controls of Tomography software (ThermoFisher). For cryo-ET of the enrichment culture ...
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bioRxiv - Cancer Biology 2023Quote: ... reagent at a 4:2:3 ratio of sgRNA/overexpression-construct: pVSVG: psPAX2 in Opti-MEM media (Life Technologies, Thermo Fisher Scientific Inc ...
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bioRxiv - Cancer Biology 2023Quote: Mouse primary myoblasts were isolated from limb muscles of pups (2-3 days old) and cultured in Ham’s F-10 nutrient mixture (Invitrogen) with 20% FBS (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... cDNAs were synthesized from 2 to 3 μg of total RNA using SuperScript IV and Oligo dT12-18 (Thermofisher) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... followed by seeding of 2-3×106 cells onto T25 flasks (Thermo-Fisher Scientific; Cat. No. 12-556-009) coated with Matrigel ...
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bioRxiv - Cell Biology 2023Quote: Mouse primary myoblasts were isolated from the limb muscles of 2-3 day-old pups and cultured in Ham’s F-10 nutrient mixture (Invitrogen) as described before.38 The culture medium was supplemented with 20% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2023Quote: ... The peptides were first concentrated on a precolumn (PepMap100 C18 3 μm; 75 μm × 2 cm; Thermo Fisher Scientific) and then separated on an EASY-Spray column (ES903 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... were maintained in a water jacketed 5 % CO2 incubator and passaged every 2-3 days using trypsin/EDTA (Gibco). 1E6 cells were seeded into 6 well plates ...
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bioRxiv - Immunology 2024Quote: ... The transduced cells were further cultured for 3 days under antibiotic selection pressure (2 μg/mL puromycin (Thermo Fisher)) until the non-transduced control cells were dead ...
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bioRxiv - Molecular Biology 2024Quote: ... and transfected with various plasmids using 3 μl Lipofectamine 3000 reagent and 2 μl P3000 reagent (both from Invitrogen). The total amount (1 μg ...
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bioRxiv - Cell Biology 2024Quote: ... The medium was changed every 2-3 days and cells were passaged at <95% confluency with 0.05% Trypsin-EDTA (Gibco).
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bioRxiv - Cell Biology 2024Quote: ... Cells that stably expressed proteins tagged with Halo7 or mGFP were established by using G418 (Nacalai Tesque, 0.2 mg/mL for PC-3 and 2 mg/mL for 4175-LuT) or blasticidin (Invitrogen, 2 mg/mL for PC-3 and 1 mg/mL for 4175-LuT) ...
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bioRxiv - Cancer Biology 2024Quote: ... CIOV1, 2, 3 cell lines (Brenton Lab, University of Cambridge, UK) were cultured in DMEM/F-12 medium (Gibco) with 10% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2024Quote: ... as the function of cell health was detected via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Invitrogen, M6494) assay (Ricci et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... Caspase-3/7 was quantified using the Apo-ONE® Homogeneous Caspase-3/7 Assay (Promega, Fisher scientific) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... 4ºC) and incubated with 50 μL of 5 μM DAF-FM (4-amino-5methylamino-2’,7’-dichlorofluorescein diacetate, Life Technologies, Eugene, OR, USA) diluted in 1X PBS for 30 min at 34 °C ...
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bioRxiv - Biophysics 2020Quote: ... Subsequently, protein samples were washed once using Zeba™ Spin desalting columns (7 kDa MWCO, 2 mL) (Thermo Fisher Scientific, Waltham, MA, USA) equilibrated with degassed buffer A ...
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bioRxiv - Biochemistry 2022Quote: ... After a clarifying spin for 15 min at 14,000 ×g and 4°C 2 μl (MICOS complex) or 7 μl (all other mitochondrial protein complexes) G-250 Sample Additive (Invitrogen, Waltham, Massachusetts, USA) containing 40% glycerol were added ...
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bioRxiv - Bioengineering 2023Quote: ... For each sample 2 μg of solubilized silk protein was loaded into a NuPAGE™ 7% Tris-Acetate gel (EA03585BOX, Invitrogen, Waltham, MA) under reducing conditions ...
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bioRxiv - Biochemistry 2023Quote: ... and transferred onto a 0.45 μm nitrocellulose membrane for 7 min at 20 V with the i-Blot 2 (Thermo Fisher, Waltham, MA, USA). Blocking ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were centrifuged for 3 min at 500 g and Accutase was replaced with 500ul assay medium containing 2μM 2’,7’ –dichlorofluorescein diacetate (DCFDA) (Thermo Fisher Scientific, cat.no. D399) and 1:1000 live-dead stain Zombie NIR (Biolegend ...
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bioRxiv - Plant Biology 2020Quote: ... peptides were reconstituted in 0.1% formic acid and 2% acetonitrile and loaded onto a trap column (C18 PepMap 100 μm i.d. × 2 cm trapping column, Thermo Fisher Scientific) at 5 µL/min for 6 min using a Thermo Scientific UltiMate 3000 RSLCnano system and washed before switching to the analytical column (BEH C18 ...
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bioRxiv - Cell Biology 2020Quote: ... The air-dried DNA was dissolved in 19 µL of sterile water and treated with Swa1 in two cycles (2×2 FastDigest units, Thermo Scientific), each cycle of 1 hour at 37°C (total volume 25 µl ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed twice with PBS/2% FBS prior to resuspension in PBS/2% FBS supplemented with 0.5 µm Sytox blue (ThermoFisher Scientific S11348) to assess cell viability ...
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Superresolution microscopy reveals photoreceptor-specific subciliary location and function of Cep290bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies used (8 μg each): F(ab’)2-goat anti-mouse IgG Alexa 647 & F(ab’)2-goat anti-rabbit IgG Alexa 555 (Thermo Fisher). In WGA-Alexa647 labeling experiments ...
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bioRxiv - Genetics 2021Quote: ... using 2 µg of total RNA in the presence of 2 U/µl of RNaseOut Recombinant RNase Inhibitor (Thermo Fisher Scientific). Genomic DNA was eliminated using the RapidOut DNA Removal Kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was treated with or without (Mock) RNase R (2 U/μg) in the presence of Ribolock(2 U/μg) (ThermoFisher Scientific). The reaction was incubated at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: Approximately 60 WT and MafAS64F/+ islets from at least 6 female and male mice were analyzed with the ratiometric calcium indicator fura-2-acetoxymethylester (Fura-2 AM) (Life Technologies). Islets were maintained in 5 mM glucose for 30 min prior to measuring 11 mM glucose-induced calcium oscillations and depolarization-activated calcium influx with 30 mM KCl ...
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bioRxiv - Immunology 2021Quote: ... We generatead F(ab’)2 fragments using Pierce™ Mouse IgG1 Fab and F(ab’)2 Preparation Kit (ThermoFisher Scientific #44980). ROS production upon stimulation with or without 10 μM peptide S100A6-1 coated to a Microfluor® 2 white-bottom 96-well plate (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... the concentrations of human IL-2 and IFN-γ were measured using a IL-2 Human Uncoated ELISA Kit (Thermo Fisher) and IFN-γ Human Uncoated ELISA Kit (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: Biotinylated heparin was prepared by mixing 2 mg of heparin and 2 mg of amine– PEG3–Biotin (Thermo Scientific, Waltham, MA) with 10 mg of sodium cyanoborohydride (NaCNBH3 ...
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bioRxiv - Neuroscience 2021Quote: ... the slices were decolored with 100% ethanol for 2 minutes and incubated for 2 minutes in xylene before mounting with Permount mounting medium (Fisher scientific). Brain slices were digitized using a Zeiss Axio Scan.Z1 slide scanner (Carl Zeiss ...
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bioRxiv - Genomics 2021Quote: ... 1 million cells in 6-well plates were transfected with 2 μg of plasmid pT2_FF together with 2 μg of plasmid pCMV(CAT)T7-SB100x and 2 μg of plasmid pcDNA3.1-mCherry using Lipofectamine 2000 (Thermo Fisher, #11668027). After 24 h ...
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bioRxiv - Microbiology 2021Quote: ... Activation of CD4+ T cells was achieved by addition of 25 U/ml of interleukin-2 (IL-2) and CD4+ T-cell activation Dynabeads (Life Technologies). CD4+ T-cells activated for 4-5 days were used for HIV-1 infections ...
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bioRxiv - Microbiology 2021Quote: ... The resulting peptides were collected on a trap column (2 mm x 2 cm) filled with POROS 20 R2 material (Thermo Scientific) kept at 0.5 °C ...
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bioRxiv - Microbiology 2020Quote: ... 24 h later the dish was washed with PBS and treated with PR8 virus (MOI =2) in the infection medium [F-12K with 2% BSA fraction V (Thermo Fisher), 1% antibiotic-antimycotic (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... The concentration of IL-2 in 2x diluted supernatants was measured using an IL-2 mouse ELISA kit (Thermo Fisher Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Peptides were reconstituted in 0.1% formic acid and 2% acetonitrile and loaded onto a trap column (C18 PepMap 100 μm i.d. × 2 cm trapping column, Thermo Fisher Scientific) at 5 µL/min for 6 min using a Thermo Scientific UltiMate 3000 RSLCnano system and washed for 6 min before switching the precolumn in line with the analytical column (BEH C18 ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated in RP-10 supplemented with 10 ng/ml recombinant human IL-2 and 2 μg/ml anti CD28 (clone 37.51, Thermo Fisher Scientific) for 18-22 hours ...
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bioRxiv - Biophysics 2022Quote: ... the glass coverslips were mounted onto a Warner chamber and the cells were loaded with 2 µM Fura-2/AM (Molecular Probes) in Ca2+ Imaging Buffer (Ca2+ IB;10 mM HEPES ...