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Citations for Thermo Fisher :
5851 - 5900 of 10000+ citations for 7 Chloromethyl 2 2 dimethyl 2 3 dihydro 1 benzofuran since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... proteins were transferred to a nitrocellulose membrane (iBlot 2 Transfer Stacks, mini) in an iBlot 2 Gel Transfer Device (Invitrogen; Carlsbad, CA). Immunodetection was carried out using commercially available primary antibodies against PCCA (1:500 ...
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bioRxiv - Biochemistry 2023Quote: ... To initiate reaction, 200 µL of Assay Buffer with substrate mixture (30 mM K3PO4, 2 mM MgSO4, 2 mM DTT (Fisher Scientific, AAJ1539706), 0.56 mM TPP (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were loaded onto a 2 cm pre-column (C18, 5 μm, 100 A°, 100 μ, 2 cm Nano-viper column # 164564, Thermo Scientific) at 5 μl/minute flow rate using loading pump for about 5 minutes and then resolved the peptides on a 50 cm analytical column (C18 ...
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bioRxiv - Cell Biology 2023Quote: ... on homemade coverslip-bottomed dishes were washed and loaded with Fura 2 by incubation with Fura 2 AM (4 μM, 45 min, 37°C, ThermoFisher, Cat# F1221), washed and imaged at room temperature in HEPES-buffered saline solution (HBSS) ...
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bioRxiv - Genomics 2023Quote: ... We combined 2 µg of the plasmid mixture with 2 µl of Lipofectamine™ Stem transfection reagent (Thermo Fisher Scientific, cat# STEM00008) and 100 µl of Opti-MEM I (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... sub-confluent HEK293T cells in 24-well plates were transfected with 500 ng of the psiCHECK-2 reporter plasmids mentioned above (G4Q27, G4M27, and native psiCHECK-2) using Lipofectamine 3000 (Invitrogen, ThermoFisher Scientific) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal sections (25 μm thick) from Bregma −2 to +2 (Paxinos and Watson, 2007) were collected onto Superfrost Ultra Plus slides (Thermo Scientific, Germany) air dried at least 2 h at room temperature (RT ...
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bioRxiv - Genomics 2023Quote: ... plugs were melted for 2 minutes at 70°C and solubilized with 2 μL of 0.5 U/μL AGARase enzyme (ThermoFisher Scientific, Waltham, MA) for 45 minutes at 43°C ...
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bioRxiv - Microbiology 2023Quote: ... the cultures were co-transfected with 2 µg of pCHMP-NS3-Flag and 2 µg of pGag using 4 µL of 293Fectin Reagent (Thermo Fisher Scientific). Cells were pelleted and high-pressure frozen as described in [68] using an HPM100 system (Leica Microsystems) ...
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bioRxiv - Molecular Biology 2023Quote: ... HeLa (CRM-CCL-2) and U-2 OS (HTB-96) cells obtained from ATCC were cultured in DMEM (Thermo Fisher Scientific, 12800017) supplemented with 10% fetal bovine serum (Lonsera ...
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bioRxiv - Cell Biology 2024Quote: ... Tryptic peptides (2 µg) were loaded onto a C18 trap (75 µm × 2 cm; Acclaim PepMap 100, P/N 164946; Thermo Fisher Scientific) at a flow rate of 2 µL/min of solvent A (0.1% formic acid and 3% acetonitrile in LC-mass spectrometry grade water) ...
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bioRxiv - Synthetic Biology 2024Quote: ... subtilis LLO was centrifuged (10,000 x g) for 2 min then resuspended in CellTracker Orange CMRA Dye (CTO, Invitrogen, C34564, 2 µM) in PBS then incubated at 37°C and 250 RPM for 25 min ...
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bioRxiv - Systems Biology 2024Quote: ... Tryptic peptides (2 μ were loaded onto a C18 trap (75 um x 2 cm; Acclaim PepMap 100, P/N 164946; Thermo Scientific) at a flow rate of 2μl/min of solvent A (0.1% formic acid in LC-MS grade water) ...
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Functional and epitope specific monoclonal antibody discovery directly from immune sera using cryoEMbioRxiv - Immunology 2024Quote: ... Cells were resuspended in phosphate buffered saline(PBS) + 2% fetal bovine serum(FBS) 200 ng/mL 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific). Cell sorting was performed on a BD FACSAria Fusion (BD Biosciences).
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bioRxiv - Cell Biology 2024Quote: ... The click reaction was performed for 2 hrs at room temperature using 2 μM of the Alexa647 alkyne (Thermo Fisher Scientific, A10278), in a copper-mediated click reaction with CuSO4 ...
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bioRxiv - Immunology 2021Quote: ... Anti-mouse SARS-CoV-2-spike (GTX, 1:100) and anti-rabbit hACE2 (Thermo Fisher,1:100) were applied overnight at 4 °C followed by incubation of appropriate secondary antibodies conjugated with fluorophores ...
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bioRxiv - Molecular Biology 2021Quote: ... at a ratio of 1:2:1 using the transfection reagent (Lipofectamine 3000, Thermo Fisher Scientific #L3000001). Six hours after the transfection ...
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bioRxiv - Developmental Biology 2020Quote: mESC lines were cultured in 2-inhibitors based medium (Advanced DMEM/F12:Neurobasal (1:1) medium (Gibco), supplemented with 2.5% ESC-grade fetal bovine serum (vol/vol ...
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bioRxiv - Genetics 2022Quote: ... samples were denatured in 1:1 with 2 x SDS buffer with 1x reducing agent (Invitrogen, NP0004) for 10 min at 95 °C ...
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bioRxiv - Immunology 2020Quote: ... gp120 in PBS (200μg/ml for 100μl/20μg/mice) was mixed at a 1:1 ratio with Alhydrogel 2% (Invitrogen) and injected intraperitoneally ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 1% antibiotic-antimycotic and 1% protein-free hybridoma medium II (PFHM 2) (Thermo Fisher Scientific) and the respective growth factors and small molecules (8 µM GSK-3 inhibitor CHIR99021 (R&D Systems) ...
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Glycogen Synthase Kinase 3 regulates the genesis of the rare displaced ganglion cell retinal subtypebioRxiv - Neuroscience 2021Quote: ... Sections were counterstained with 1:1000 4′,6-diamidino-2-phenylindole (DAPI) (1 mg/mL, Thermo Scientific).
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bioRxiv - Molecular Biology 2023Quote: ... and BE(2)-C were cultured in a 1:1 mixture of DMEM/F12 (with HEPES, Gibco) and EMEM (ATCC) ...
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bioRxiv - Biophysics 2023Quote: ... A preheated (90°C, 1 min) number 1 German borosilicate sterile 2 well chamber coverglass slide (ThermoFisher) was loaded with 750 μL of melted 0.7% Omnipur agarose (EMD Millipore) ...
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bioRxiv - Plant Biology 2024Quote: ... Seeds were sown on plates containing 1/2 strength MS and 1% BD Difco Agar (Fisher Scientific) at a pH of 5.8 ...
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bioRxiv - Cancer Biology 2024Quote: ... Gibco 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (1 M) was obtained from Invitrogen (Carlsbad, CA) and sterile filtered before use ...
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bioRxiv - Cell Biology 2021Quote: Intracellular levels of reactive oxygen species (ROS) were measured using the cell-permeant redox-sensitive probe 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Molecular Probes, Thermo Fisher Scientific, USA). HEK 293 cells were grown in a 96-well plate for fluorescent-based assays until ∼80% confluence ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Fecal samples were immediately transferred to an anaerobic chamber within 2 h after defecation and suspended at 20% (w/v) in reduced peptone water (0.1%, pH 7; Thermo Fisher Diagnostics AG, Pratteln, Switzerland). Immobilization of 10 ml fecal slurry in polymer gel beads (gellan gum (2.5% ...
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bioRxiv - Cancer Biology 2023Quote: ... Tris-Glycine 10-20% or Bis-Tris 4-12% SDS-PAGE gels were run at 4℃ and proteins were transferred onto nitrocellulose membranes using 15 Volts for 7 minutes via the iBlot-2 system (Thermo Fisher Scientific, Waltham, MA). The membrane was then blocked for 1 hour in LICOR Odyssey blocking buffer and incubated at 4℃ with the appropriate antibody overnight ...
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bioRxiv - Plant Biology 2024Quote: The production of ROS in guard cells of CrSAL1 transgenic and WT plants was measured using a fluorescent indicator 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA, Life Technologies, Waltham, MA USA) (Cai et al. ...
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bioRxiv - Cell Biology 2020Quote: ... and then loaded with fluorescent Ca2+ probes (3 μM of fura-2 AM or 5.69 μM of Fluo-4 AM, Life Technologies) in HBSS for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... A Neuropixels probe (option 3 phase A) with 374 recording sites was briefly (∼2 minutes) dipped into the diI cell-labeling solution (ThermoFisher) to visualize probe tracks ...
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bioRxiv - Molecular Biology 2020Quote: ... 2:6 and 3:6 dilution ratios to allow efficient selection of Hygromycin B (Thermo Fisher Scientific Catalog Number: 10687010). The Hygromycin selection was started at the 48 hours after transfection time point with a final concentration of 150µg/ml and refreshed every 3-4 days until the control non-transfected cells on a separate plate were completely dead (takes approximately 3 weeks from the start of transfection until the cells are expanded and frozen) ...
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bioRxiv - Molecular Biology 2020Quote: ... medium from the basolateral compartment was renewed every 2–3 days and the apical surface was washed once a week with PBS (Invitrogen).
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bioRxiv - Neuroscience 2020Quote: ... third instar larvae were dissected in zero Ca2+ HL-3 solution at room temperature and stimulated with a HL-3 solution of 90 mM K+/2 mM Ca2+/4 μM FM4-64 (Invitrogen) for 5 min to load FM4-64 dye into the boutons ...
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bioRxiv - Genetics 2020Quote: ... samples were diluted in Laemmli sample buffer and 2 μg of protein was separated on a NuPage 3–8% Tris-Acetate gel (Invitrogen). The gel was then stained with Coomassie Blue and digitalized.
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bioRxiv - Genetics 2021Quote: 2-3 × 106 mESCs were transfected with 14.5 μg of TALE plasmid and 26 μL Lipofectamine 3000 Reagent (Invitrogen L3000015) and seeded onto 0.1% gelatin-coated 10 cm dish containing an autoclaved SuperFrost Plus Adhesion glass slide ...
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bioRxiv - Bioengineering 2020Quote: ... proteins and the Canadian clinical isolate of SARS-CoV-2 (V-2/Canada/VIDO-01/2027 Feb 2020) were mixed in fresh media supplemented with 3% fetal bovine serum (Gibco) and preincubated for 1 hour at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated overnight at 4°C in blocking solution (PBS, 2% normal goat serum, 3% BSA) and primary antibodies (mouse anti-HA, Invitrogen; rabbit anti-GFP, Invitrogen). Cells were then immunolabeled with Alexa-conjugated secondary antibodies (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed twice with PBS prior to loading with 3 μM of the Ca2+ indicator Fura-2 AM (Invitrogen) diluted in a modified Krebs-Ringer buffer [KRBH ...
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bioRxiv - Pathology 2021Quote: ... The tissue was homogenized with a Pasteur pipette in Growth medium [3 ml DMEM-F12 with 2% of B27 without vitamin A (Gibco), 20 ng.mL-1 Epidermal Growth Factor (EGF ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was produced from 2-3 g total RNA as described (Greenstein et al 2018) using a dT primer and Superscript IV (Invitrogen) reverse transcriptase ...
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bioRxiv - Cancer Biology 2020Quote: ... Total RNA was isolated from sorted 2-3×10^5 CD34+ cells using the mirVANA miRNA isolation kit (Thermo Fisher) and subsequently processed using the Small RNA Library Prep kit (Norgen Biotek) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were loaded with 3 μM Fura-2-AM and 0.01% (v/v) Pluronic F-129 (both from Molecular Probes) in DMEM medium at 37 °C for 20 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were treated with RNAScope H2O2 for 4 min at RT and subsequently washed 2 x 3 min with UltraPure Distilled Water (Invitrogen) at RT ...
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bioRxiv - Systems Biology 2022Quote: ... Transcriptomics using the isolated mRNA from liver tissues (0, 2, 4, 6, 8 and 10 weeks; n=3 per time point) was performed by Affymetrix GeneChip®Mouse Gene 2.0 ST Arrays (902118) ...
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bioRxiv - Microbiology 2022Quote: Cell toxicity due to the compound treatment was determined using the standard colorimetric MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Parasitemia was monitored daily by flow cytometry by diluting 10 μl of each parasite culture well from 2-3 biological replicates into 200μl of 1.0 μg/ml acridine orange (Invitrogen Life Technologies A3568) in phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2021Quote: ... and 2 µL of total RNA was subjected to One-Step RT-qPCR using Applied Biosystems QuantStudio 3 (ThermoFisher Scientific), with the following cycling conditions ...
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bioRxiv - Immunology 2022Quote: The uptake and processing of CSF-derived protein by antigen-presenting cells outside the CNS was evaluated by injecting 3 μl of DQ-OVA (2 mg/ml, Invitrogen) into the CSF of chronically infected C57BL/6 mice by i.c.m ...