Labshake search
Citations for Thermo Fisher :
5651 - 5700 of 10000+ citations for 6 Benzothiazolamine 2 1 methylpropyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... N2a cells were transfected with pSpCas9(BB)-Puro empty vector or vectors expressing each of the three gRNAs targeting the eIF4E3 exons (meIF4E3 gRNA exon 1, exon 2, exon 4) using Lipofectamine 2000 (Invitrogen). pSpCas9(BB)-Puro was a generous gift from Dr ...
-
bioRxiv - Cell Biology 2021Quote: ... and then stained for 30 min with LipidTox Red (1:1000 dilution) or for 15 min with BODIPY 493/503 (2 μg/ml) (Invitrogen) to visualize LDs ...
-
bioRxiv - Immunology 2021Quote: ... 1-2 × 105 cells were washed with facs wash (FW, PBS supplemented with 1% hiFCS and 0.05 mM EDTA (ThermoFisher Scientific)) and stained in FW with antibodies either 30 min on ice or 15 min at room temperature in the dark ...
-
bioRxiv - Genetics 2019Quote: ... Cells were washed thrice with PBS and stained with secondary antibodies (in 2% BSA/PBS) for 1 hour at room temperature (all Invitrogen at 1:2000 ...
-
bioRxiv - Genomics 2021Quote: ... tumors and adjacent non-cancer tissues were cut into approximately 1-2 mm3 pieces in the RPMI-1640 medium (Gibco), and enzymatically digested with gentleMACS (Miltenyi ...
-
bioRxiv - Genetics 2020Quote: ... maintenance in mTesR1 medium for 1-2 days was required before puromycine selection (0.8μg/ml puromycine [ThermoFisher] for 24 hours). 24-hour puromycine selection was made for every passage.
-
bioRxiv - Neuroscience 2020Quote: ... We used a Nanoject II to inject 0.2 – 1 μl of 2% WGA-Alexa Fluor 555 (Thermo Fisher Scientific; #W32464) and 0.5% Fast Green (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2021Quote: ... Spleens were then explanted and placed into a 2 mL Eppendorf tubes filled with 1 mL of RPMI 1640 (Gibco), 50 μM 2-mercaptoethanol (Sigma) ...
-
bioRxiv - Immunology 2020Quote: ... cells were resuspended at a concentration of 1-2×106/mL in 50-100 µL of cold HBSS containing 5% heat-inactivated FBS (Gibco), 100U/mL penicillin ...
-
bioRxiv - Microbiology 2022Quote: ... 2 mM l-glutamine, 1 mM sodium pyruvate, 30% L929-conditioned medium, 20% heat-inactivated fetal bovine serum [FBS; Invitrogen] ...
-
bioRxiv - Developmental Biology 2022Quote: ... secondary antibodies were applied for 2 h in the dark (1:250 anti rabbit IgG coupled to AlexaFluor-555 [Invitrogen, A31572] and 1:250 anti mouse IgG coupled to AlexaFluor-488 [Invitrogen ...
-
bioRxiv - Immunology 2022Quote: ... The sorted cells were then incubated with Totalseq-C antibodies (1:50 dilution) on ice for 30 min, washed 3 times (by default, top up with 2% FBS (Gibco) in DPBS (Sigma ...
-
bioRxiv - Cancer Biology 2022Quote: ... T cells were then resuspended at 2 × 106 cells/ml in PBS and stained with Cell Trace Violet (1:2000, Invitrogen) for 20 minutes at 37°C ...
-
bioRxiv - Biochemistry 2022Quote: ... Eluates were diluted to 2 M urea in 50 mM ammonium bicarbonate and digested with 1 μg of trypsin (ThermoFisher) overnight at 37°C ...
-
bioRxiv - Cell Biology 2022Quote: ... Switzerland) and Hoechst 33342 dye (1 drop/2 ml of NucBlue Live ReadyProbes Reagent, Thermo Fisher Scientific, Waltham, MA, USA) and have been confirmed to be mycoplasma free.
-
bioRxiv - Molecular Biology 2022Quote: ... and then treated with 0.4 mg/ml proteinase K (1-2 hour, 50°C) before addition of 25 μM Sytox Green (ThermoFisher Scientific). Cells were then sonicated and DNA-content was analysed for >10,000 events on either a BD Biosciences FACScan Analyzer or an Attune NxT flow cytometer ...
-
bioRxiv - Bioengineering 2022Quote: ... Wells were rinsed with PBS and CD8 T cells were added at 1×106 cells/mL in complete RPMI supplemented with 50 U/mL IL-2 and mouse T-activator Dynabeads (ThermoFisher) at 1:1 ratio ...
-
bioRxiv - Cell Biology 2022Quote: ... LECs were seeded into 0.1% gelatin (214340; Difco)-coated wells (1 lung/2 cm2) and cultured in DMEM plus GlutaMAX (10564011; ThermoFisher Scientific), 20% fetal bovine serum (Hyclone) ...
-
bioRxiv - Cell Biology 2022Quote: ... Skeletal muscle were stained with Myosin 4 monoclonal antibody (MF20, MyHC-2) (1:200; Thermo Fisher Scientific, Rockford, IL, USA) and Laminin gamma 1 Antibody (A5 ...
-
bioRxiv - Immunology 2022Quote: ... resuspended in solubilization buffer (9M Urea containing 1% DTT and 2%Triton X and NuPAGE lithium dodecyl sulfate sample buffer 4X (Invitrogen) at a 3:1 ratio) ...
-
bioRxiv - Immunology 2022Quote: ... Cells in suspension were harvested on day 0 and both floating and adherent cells were harvested on day 2 in 1 mL TRIzol (Invitrogen). Total RNA was extracted using chloroform ...
-
bioRxiv - Genomics 2022Quote: ... resuspended in 1mL nuclei wash buffer (2% BSA and 0.2 U μl−1 RNase inhibitor (Thermo Fisher, cat. no. AM2694) in 1× PBS ...
-
bioRxiv - Microbiology 2022Quote: ... Cells in a 6-well plate were transfected with 2 μg of pTM1 P and 1 μg wt or mutant L using Lipofectamine 2000 (Invitrogen), according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... Tissue was washed and incubated for 2 hr at room temperature in goat anti-rabbit IgG conjugated to Alexa 594 (1:500, Invitrogen). Sections were again washed ...
-
bioRxiv - Microbiology 2022Quote: ... 1 μl of Alexa Fluor 488 goat anti-mouse IgG (H+L) at 2 µg/ml (Life Technologies, cat#A11001) and 0.5 μl of 10 mg/ml Hoechst 33342 was added to the sample and incubated for 15 minutes at RT in the dark ...
-
bioRxiv - Immunology 2022Quote: ... 106 splenocytes were ex vivo stimulated with SARS-CoV-2 peptide pool (JPT PM-WCPV-S-1) at concentration of 2 μg/ml of each peptide in RPMI (Biowest) supplemented with 10% heat inactivated FBS (Gibco). PMA/Ionomycin (BD Pharmingen™ ...
-
bioRxiv - Microbiology 2022Quote: ... Staining was carried out at 37 °C for 15 min and then 2 μL of each culture was transferred on a microscope slide with a pad of 1 % UltraPure agarose (Invitrogen) in H2O ...
-
bioRxiv - Microbiology 2022Quote: ... 1% penicillin-streptomycin antibiotic solution and 2 mM of L-Glutamine (all reagents were obtained from Gibco, Waltham, MA, USA). Cell lines were incubated at 37 °C under a 5% CO2 atmosphere ...
-
bioRxiv - Microbiology 2022Quote: ... The next day, the larvae were washed (3×, 1× PBST) and incubated for 30 minutes in 2 μg/mL Hoechst 33342 (Invitrogen) in 1× PBT at room temperature ...
-
bioRxiv - Neuroscience 2022Quote: ... cells were incubated for 1 min with F(ab’)2-Goat anti-Rabbit IgG (H+L) Secondary Antibody QDot emitting at 655 nm (1 nM; Invitrogen) in PBS (1 M ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cell differentiation was induced with differentiation media (DMEM supplemented with 2% horse serum, 1% Insulin-Transferrin-Selenium-A supplement (Invitrogen) and 1% penicillin-streptomycin ...
-
bioRxiv - Neuroscience 2022Quote: ... tissues were washed with PBS and incubated for 2 h with secondary antibodies conjugated to AlexaFluor 488 (1:400; Invitrogen). Sections were then rinsed with PBS ...
-
bioRxiv - Plant Biology 2022Quote: ... PhnsLTP3-RNAi and PhnsLTP1+ PhnsLTP3-RNAi flowers collected at 15:00 h on day 2 postanthesis were stained in propidium iodide solution (10 μg mL-1, Invitrogen) for 1 h at room temperature with shaking ...
-
bioRxiv - Plant Biology 2023Quote: ... Each sample 1/20 of digests) was loaded onto a fused silica trap column (Acclaim PepMap 100, 75 μm × 2 cm, ThermoFisher). After washing for 5 min at 5 μl/min with 0.1% TFA ...
-
bioRxiv - Immunology 2022Quote: PCR products (1-2 ng per 100 base pairs) were treated with ExoSAP-IT PCR Product Cleanup Reagent (Life Technologies) at a ratio of 5:2 at 37°C for 4 minutes ...
-
bioRxiv - Biochemistry 2022Quote: ... 2 μg of total RNA harvested from parental RPE-1 and D12 cells utilizing the TRIzol reagent (ThermoFisher Scientific, 15596026) was used for cDNA synthesis (Applied Biosystems ...
-
bioRxiv - Neuroscience 2022Quote: ... Sections were rinsed 3 times in TBS for 10 min each and incubated for 1 h at room temperature with a combination of 2 fluorescent dye-labelled secondary antibodies: a Cy3-conjugated goat anti-rabbit antibody (1:200; Invitrogen, Waltham ...
-
bioRxiv - Neuroscience 2022Quote: ... counterstained with 1 μg/mL diamidino-2-phenylindole dihydrochloride (DAPI) and cover-slipped with ProLong Glass antifade (Life Technologies #P36980) before imaging ...
-
bioRxiv - Microbiology 2022Quote: ... the robot adds 2 volumes of 30% PEG 6000 (in 1.6 M NaCl) and 35ul of 1:5 diluted Glycoblue (Invitrogen, Thermo Fisher) to each well ...
-
bioRxiv - Cell Biology 2022Quote: ... and control cell lines were obtained by transduction with retroviral particles generated in Hek293T cells and sustained selection with puromycin 2 μg ml-1 (A1113803, ThermoFisher). Specifically ...
-
bioRxiv - Neuroscience 2022Quote: ... Maturation medium I was DMEM/F12 supplemented with 100 U/ml Penicillin-Streptomycin and 1% N-2 Supplement (Gibco, 17502048). On day 10 ...
-
bioRxiv - Physiology 2022Quote: ... 2×105 CD11b+ monocytic BM cells that were isolated by biotinylated anti-CD11b (1:100; clone M1/70; ThermoFisher Scientific) and anti-biotin microbeads (Miltenyi Biotec) ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1×106 cells were resuspended in 500μl FACS buffer (PBS with 2% fetal bovine serum (Gibco, 10099-141, lot 1751896)) ...
-
bioRxiv - Immunology 2022Quote: ... Purified DEL was conjugated to biotin at a 1:2 molar ratio using Sulfo-NHS-LC-Biotin (Thermo Fisher Scientific) according to the manufacturer’s directions and incubated for 3 hours on ice ...
-
bioRxiv - Biochemistry 2024Quote: ... 8 µL of 2.5X MaP Buffer was added and incubated at 42°C for 2 minutes before addition of 1 µL SuperScript II Reverse Transcriptase (ThermoFisher) and incubation at 42°C for 3 hours ...
-
bioRxiv - Immunology 2023Quote: MD39 trimer immunogens bearing a free C-terminal cysteine at a concentration of 1 mg/ml were reduced by incubation with 10 molar equivalents of tris(2-carboxyethyl)phosphine (TCEP, ThermoFisher), followed by incubation at 25°C for 10 minutes ...
-
bioRxiv - Microbiology 2024Quote: Phage DNA was isolated by treating 2 ml of Phage solution with 1 U/μL DNAse (Invitrogen, Carlsbad, CA, USA) to remove free DNA ...
-
bioRxiv - Microbiology 2024Quote: ... and differentiated using 2.5% FBS neurobasal media (1X B27, 20 mM KCl, 1% Pen-Strep, 2 mM Glutamax (Thermo Fisher) 50 ng/mL Brain-derived neurotrophic factor (BDNF) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Digestions were incubated for 1 hour at 37 ℃ and then diluted 1:5 with UltraPure water before loading on E-gel EX 2% Agarose gels (Invitrogen). Backbones were purified using the Monarch Gel Extraction Kit (Monarch) ...
-
bioRxiv - Molecular Biology 2024Quote: ... cDNA was generated from 1-2 mg of total RNA using random hexamers and Maxima Reverse Transcriptase (Thermo Fisher Scientific). Quantitative PCR was performed on the ViiA 7 Real-Time PCR System (Life Technologies ...