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Citations for Thermo Fisher :
5701 - 5750 of 10000+ citations for Anti NKCC1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... Membrane damage was assessed using anti-IgG staining using an anti-rat antibody (IgG H+L AlexaFluor® 647 1:100, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Coverslips with fixed cells were then washed with PBS and incubated with the secondary antibodies Alexa-fluor™ 488 chicken anti-rabbit IgG and Alexa-fluor™ 555 goat anti-mouse IgG (Invitrogen) for 45 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... the membrane was incubated with a fluorescently labelled-secondary antibody (Goat anti-Rabbit Alexa Fluor 680 #A32734 against STAT1; Goat-anti-Mouse Alexa Fluor 800 #A32730 against ACTB) (Thermo Fisher Scientific), Massachusetts ...
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bioRxiv - Cell Biology 2021Quote: ... after which they were rinsed three-times with PBS and stained for 45 min with Alexa-Fluor-594- and Alexa-Fluor-488-conjugated anti-rabbit or anti-mouse secondary antibodies (Thermo Fisher Scientific). Nuclei were visualized by staining with 1 μg/ml Hoechst dye 33258 (Sigma-Aldrich).
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bioRxiv - Cell Biology 2021Quote: ... heart sections were incubated with rabbit anti-TFEB (SC48784, 1:500 dilution) antibody for 1 h and subsequently Alexa Fluor 568 goat anti-rabbit (Invitrogen, A-21069) or Alexa Fluor 488 goat anti-mouse (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... followed by incubation with fluorescein isothiocyanate-labeled goat anti-rabbit and anti-mouse secondary antibody (Alexa Fluor; Invitrogen; Thermo Fisher Scientific, Inc.). DAPI was applied to each section for 10 min to counterstain the nuclei.
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bioRxiv - Cell Biology 2021Quote: ... followed by incubation with fluorescein isothiocyanate-labeled goat anti-rabbit and anti-mouse secondary antibody (Alexa Fluor; Invitrogen; Thermo Fisher Scientific, Inc.). DAPI was applied to each section for 10 min to counterstain the nuclei.
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bioRxiv - Cell Biology 2022Quote: ... coverslips were incubated for 2 hours in a 1:1000 dilution of secondary antibody (anti-rabbit, Invitrogen, 32260; anti- mouse, Invitrogen, 32230). Coverslips were washed 3 times with blocking buffer ...
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bioRxiv - Biochemistry 2022Quote: ... and Alexa Fluor 488- or 568-labeled donkey anti-rabbit or goat anti-mouse secondary antibodies (1:1,500 dilution, Life Technologies, A10042, A11029), and mounted with DAPI-containing VECTASHIELD mounting medium (Vector Labs) ...
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bioRxiv - Biochemistry 2022Quote: ... followed by incubation with DAPI (1:1000) and conjugated secondary antibodies anti-mouse Alexa555 and anti-rabbit Alexa647 (1:500, Thermo Fisher Scientific). Coverslips were mounted and samples were analyzed using a Leica SP8 Laser Scanning Confocal Microscope with a 63x oil objective (Leica ...
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bioRxiv - Cancer Biology 2022Quote: ... 1:200) and then with secondary antibodies (Alexa Fluor 488 Goat Anti-Rabbit and Alexa Fluor 555 Donkey Anti-Mouse, Thermo Fisher Scientific) for 30 min at room temperature and in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies were a goat anti-mouse Alexa Fluor® 488 and a goat anti-rabbit Alexa Fluor® 594 (Invitrogen™) diluted at 1:2000 in PBS containing 0.3% Triton X100 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were the incubated with secondary antibodies goat anti-rabbit Alexa 568 and goat anti-mouse Alexa 488 (1:500, Thermo Fisher Scientific), or TRITC-labeled phalloidin (1:100 Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... for 24 h and the secondary antibodies (anti-rabbit IgG-conjugated Alexa Fluorochrome or anti-mouse IgG conjugated Alexa Fluorochrome, Invitrogen; 1:500) for 2 h at room temperature.
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bioRxiv - Cell Biology 2021Quote: ... slides were incubated in a wet chamber with the secondary antibodies (Alexa Fluor Goat anti-Rat 594, Invitrogen, 1:1000 and Alexa Fluor Goat anti-Guinea Pig, Invitrogen, 1:1000) in PBS/Triton/BSA for 1 h at room temperature in the dark ...
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bioRxiv - Pathology 2021Quote: ... blots were incubated with an anti-influenza A NP protein antibody 19C10 generated by our laboratory (1:1000) and anti-V5 tag antibody (Thermo,1:5000) and visualized with horseradish peroxidase (HRP)-conjugated anti-mouse IgG (Invitrogen, 1:5000).
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bioRxiv - Microbiology 2021Quote: ... Neutrophils were labelled with 2.5 μg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G, clone 1A8, Biolegend, #127620 + DyLight550 Antibody Labelling Kit, ThermoFisher Scientific, #84530) 15 min prior to recordings ...
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bioRxiv - Microbiology 2021Quote: ... 15 μg Dylight488-conjugated anti-mouse CD31 (LEAF purified anti-mouse CD31, clone 390, Biolegend, #102412 + DyLight488 Antibody Labelling Kit, ThermoFisher Scientific, #53024) and 2.5 μg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G ...
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bioRxiv - Microbiology 2021Quote: ... the murine vasculature was labelled with 15 μg Dylight488-conjugated anti-mouse CD31 (LEAF purified anti-mouse CD31, clone 390, Biolegend, #102412 + DyLight488 Antibody Labelling Kit, ThermoFisher Scientific, #53024) and the human vasculature with 100μg of Dylight755-conjugated UEA-1 lectin (Unconjugated UEA-1 lectin ...
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bioRxiv - Microbiology 2021Quote: ... and 2.5 μg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G, clone 1A8, Biolegend, #127620 + DyLight550 Antibody Labelling Kit, ThermoFisher Scientific, #84530) were injected to label and visualize the human vessels ...
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bioRxiv - Cancer Biology 2021Quote: ... in 1X PBS for overnight before staining with primary antibodies diluted in blocking buffer for 16 h followed by Alexa Fluor 488- or Alexa Fluor 594-conjugated goat anti-mouse or goat anti-rabbit secondary antibodies (Invitrogen, Carlsbad, CA.) for 1 h ...
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bioRxiv - Cancer Biology 2021Quote: ... incubated with Alexa Fluor 594-conjugated anti-rabbit IgG or Alexa Fluor 488-conjugated anti-mouse IgG antibodies (1:1000; Life Technologies, France) for 2 h at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Spain)] and secondary antibodies [Alexa Fluor 568 goat anti-rabbit IgG or Alexa Fluor 647 goat anti-rabbit IgG (Life Technologies, CA, USA)] were incubated for 30 min at RT ...
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bioRxiv - Biochemistry 2021Quote: ... Blots were incubated in primary antibodies (sheep anti-BPNT2, 1:1000, Invitrogen #PA5-47893; mouse anti-Actin, 1:1000, Invitrogen #MA1-744) overnight at 4C ...
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bioRxiv - Neuroscience 2020Quote: ... Then the samples were washed with washing buffer and incubated in secondary antibody: Alexa Fluor 546 anti-goat and Alexa Fluor 488 anti-rabbit IgG (1:200, ThermoFisher Scientific, USA) for 1hr ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-PV, 1:2000, ref. 235, Swant, Switzerland; secondary antibody: Alexa 488 goat anti-mouse, 1:500, ref. A11029, Life Technologies; USA) and using the virus reporter mCherry co-labelling.
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bioRxiv - Developmental Biology 2022Quote: ... The samples were then incubated in secondary antibody (donkey anti-mouse Alexa Fluor 488, A-21202 and anti-goat Alexa Fluor 647, A21447, ThermoFisher, 1:250), in 5% donkey serum and 0.5% PBST for 1 hour at RT on a shaker followed by O/N at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... membranes were incubated for 1 h at room temperature (RT) with primary antibodies (anti-core-LPS A68/24D08/G09 and anti-3Flag FG4R (Thermo Fisher Scientific), 1:100 ...
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bioRxiv - Cell Biology 2022Quote: ... The signals were detected by secondary HRP-conjugated antibodies (Anti-mouse, Cat. #7076; Anti-rabbit, Cat. #7074; CST) and either Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific) or Forte (Immobilon) ...
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bioRxiv - Developmental Biology 2022Quote: ... Secondary labeling was performed using horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG antibodies (1:3,000, both ThermoFisher Scientific, 31430, 31460) for 1 hour at room temperature ...
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bioRxiv - Physiology 2020Quote: ... Brain sections stained with guinea pig anti-tdTomato antibody (1:1,000; Frontier Institute) were incubated with biotin-labelled anti-guinea pig IgG (1:250; Invitrogen, Carlsbad, CA, USA) overnight ...
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bioRxiv - Bioengineering 2019Quote: ... Slides were rinsed with PBS before the secondary antibody (Alexa Fluor® anti-goat IgG and anti-rabbit IgG, Invitrogen; 1:200) was diluted in 1 % BSA and applied for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... the brain slices were incubated with the secondary antibodies (anti-rabbit IgG-conjugated Alexa Fluorochrome or anti-mouse IgG conjugated Alexa Fluorochrome, Invitrogen; 1:500) for 2 h at room temperature.
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed three times PBS and incubated in anti-mouse or anti-rabbit fluorescently conjugated secondary antibodies (Invitrogen Life Technologies, RRID:SCR_008410) diluted in blocking buffer (1 ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 h before incubation with primary antibodies (1:1000 anti-tyrosine hydroxylase, #AB152, Millipore UK; 1:1000 anti-GFP, #A10262, Fisher Scientific, UK) in blocking solution at room temperature on a shaker for 18 h ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed three times with PBS and incubated for 30 min with secondary antibody (AlexaFluor488-conjugated goat anti-mouse Thermo Fisher #A32723 and/or AlexaFluor594-conjugated donkey anti-rabbit Thermo Fisher #A32754 both at 1:500 dilutions in blocking buffer) ...
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bioRxiv - Physiology 2021Quote: ... The signals were detected by secondary HRP-conjugated antibodies (Anti-mouse, Cat. #7076; Anti-rabbit, Cat. #7074; CST) and Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific). Protein band intensities were quantified with Image Studio Lite software (LI-COR).
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bioRxiv - Developmental Biology 2021Quote: ... As secondary antibodies were used: donkey anti rabbit FP 647H (Interchim, 1:1000) and donkey anti goat Alexa555 (Molecular probes, 1:1000). In the case of the co-localization ...
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bioRxiv - Microbiology 2020Quote: ... incubating with primary antibodies (rabbit anti-β-actin, 1:1000, Thermo Fisher Scientific, PA1-183; mouse anti-claudin-2, 3:500, Invitrogen, 325600 ...
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bioRxiv - Neuroscience 2019Quote: ... sections were rinsed with agitation three times for 5 min in 0.01 M PBS and incubated in Alexa Fluor goat anti-rabbit 594 or goat anti-mouse 488 secondary antibody (1:200; Life Technologies, CA) in 0.01 M PBS ...
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bioRxiv - Neuroscience 2020Quote: ... All steps for IHC were identical to those described above except primary antibody incubations occurred over 24 h and primary antibodies were detected with goat anti-chicken 488 and goat anti-rabbit 568 (Invitrogen; 1:500).
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bioRxiv - Cancer Biology 2021Quote: ... Slides were fixed with 4% paraformaldehyde for 10 min before addition of anti-rat Alexa Fluor 555 and anti-mouse Alexa Fluor 488 secondary antibodies (Thermo Fisher Scientific). Fluorescently-labelled DNA fibers were imaged using a Nikon Eclipse Ni microscope with NIS-Elements software (Nikon Instruments) ...
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bioRxiv - Immunology 2021Quote: ... Unlabeled primary antibodies were detected using secondary antibodies (goat anti-rat Alexa Fluor 488 or goat anti-rabbit Alexa Fluor 647; both from Thermo Fisher Scientific). Tissue was counterstained with DAPI (10pg/ml final dilution in PBS ...
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bioRxiv - Physiology 2019Quote: ... sections were incubated for 2 h at 25°C with Alexa Fluor-Plus goat anti-rabbit 555 and Alexa Fluor goat anti-chicken 647 secondary antibodies (Invitrogen, 1:1000) in PBS with 0.05% Tween 20 ...
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bioRxiv - Biophysics 2019Quote: ... The cells were then washed and incubated for 70 minutes at room temperature with Alexa 488/561-conjugated anti-rabbit and anti-mouse secondary antibodies (Molecular Probes, Invitrogen). The double labeled cells were then washed and kept a 4° C until confocal imaging ...
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bioRxiv - Neuroscience 2019Quote: ... Secondary antibodies were: Alexa-488 goat anti-mouse IgG and Cy3 goat anti-rabbit IgG (both 1:100 dilution; ThermoFisher, Leicester, UK). Brains were viewed using an Olympus FV1000 confocal microscope ...
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bioRxiv - Cell Biology 2019Quote: ... For the negative control primary antibodies mouse anti-plasmepsin V (From D. Goldberg, 1:1) and rabbit anti-GFP A-6455 (Invitrogen, 1:200) were used.
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bioRxiv - Neuroscience 2019Quote: ... Secondary fluorescently tagged antibodies were: goat anti-chicken Alexa-488 (cat. A11039) and goat anti-rabbit Alexa-647 (cat. A21244; both from Thermo Fisher Scientific), used at 1:200 dilution ...
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bioRxiv - Genomics 2021Quote: ... Appropriate secondary antibody (goat anti-mouse-Alexa-Fluor-488, goat anti-rabbit-Alexa-Fluor-488 or 568, 1:500; Invitrogen, Carlsbad, CA) was used ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were washed 3x 5 minutes in PBST then incubated with anti-rabbit and anti-mouse antibodies (LI-COR, 1:15,000 or ThermoFisher, 1:10,000) diluted in 4% milk/PBST for 1.5 h at RT ...