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Citations for Thermo Fisher :
5501 - 5550 of 10000+ citations for Anti NKCC1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... or anti-embryonic MHC (F1.652, Developmental Study Hybridoma Bank) and anti-Laminin (4H8-2, MilliporeSigma) antibodies followed by anti-mouse Alex-488 (A21202, ThermoFisher Scientific) and anti-rat Alexa-594 antibodies (A21471 ...
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bioRxiv - Biophysics 2020Quote: ... For in vitro TIRF we used surface-immobilized GFP or EGF-TMR via anti-GFP or anti-EGF antibodies (Molecular Probes) or Fab followed by BSA passivation prior to washing(24) ...
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bioRxiv - Microbiology 2019Quote: ... The viral N-protein was labeled using a primary anti-Tula virus antibody(12) and a secondary anti-rabbit FITC conjugate (Invitrogen, USA). The viral Gc protein was stained using a mouse monoclonal antibody (#H1808-60B ...
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bioRxiv - Zoology 2019Quote: ... preincubated for 30 min at room temperature in PTX+NGS and incubated overnight at 4°C in secondary antibodies (goat anti-mouse conjugated with AlexaFluor647 and goat anti-rabbit conjugated with AlexaFluor488, Life Technologies) dissolved in Ptx+NGS in 1:250 concentrations ...
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bioRxiv - Bioengineering 2019Quote: ... Mouse-anti-His6 primary antibody and goat anti-mouse IgG H&L (Alexa Fluor® 488) were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Genetics 2019Quote: ... and incubated with 1:50 mouse anti-wg (4D4, DSHB) primary antibody and 1:500 anti-mouse 488 (A-21202, Molecular Probes) secondary antibody ...
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bioRxiv - Cell Biology 2019Quote: ... Immunofluorescence staining of the sections was performed as described above and followed by anti-mouse IgG isotype specific or anti-rabbit IgG antibodies labelled with Alexa488 and Alexa555 (Molecular Probes). Samples were mounted with Prolong® Antifade reagent (Molecular Probes ...
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bioRxiv - Immunology 2020Quote: ... followed by staining with mAbs MM1a (anti-CD3) and ILA105 (anti-CD8) and secondary antibodies and Fixable Yellow Dead Cell Stain (Thermofisher Scientific). These cells were sorted using a FACSAria III (BD) ...
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bioRxiv - Immunology 2020Quote: ... gondii PVM was stained with polyclonal rabbit anti-GRA7 primary antibody (gift from John Boothroyd, Stanford University) and Alexa Fluor 488 anti-rabbit IgG (Life Technologies) at 1:3000 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... and secondary antibodies (anti-mouse IgG Alexa Flour 488 and anti-rabbit IgG Alexa Flour 488) were from Invitrogen (Carlsbad, CA).
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bioRxiv - Molecular Biology 2021Quote: ... the membranes were probed with the following primary antibodies overnight at 4°C with gentle shaking: anti-p53 (13-4100; 1:500) and anti-p21WAF1 (AHZ0422; 1:250) antibodies were purchased from Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... the cells were washed with PBS and subjected to incubation with goat anti-mouse or goat anti-rabbit secondary antibodies conjugated with Alexa Fluor 488 or Alexa Fluor 555 (Thermo Fisher) in 0.05% acetylated BSA in PBS for 2h at 37 °C ...
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bioRxiv - Genomics 2020Quote: ... Cells were then incubated with secondary antibodies (Alexa 488 anti-Rabbit, Dk, Jackson immunoResearch, 711-545-152, 1:500; Alexa 568 anti-Chicken, Gt, ThermoFisher, A-11041 ...
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bioRxiv - Pathology 2020Quote: ... The rabbit polyclonal anti-Nucleoprotein of RSV [50] and the secondary antibody Alexa Fluor 488-conjugated goat anti-rabbit IgG (A11034, ThermoFisher Scientific) were diluted at 1:500 in PBS + 10% DMSO + 0.2% triton X-100 +100 μg/mL saponin + 10 μg/mL heparin + 3% horse serum + 0.05% sodium azide ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed twice with PBS before incubation in secondary antibodies (Alexa-568-phalloidin, anti-rabbit Alexa-647, anti-mouse Alexa-588 (Life Technologies) for one hour at a dilution of 1:300 in PBS.
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bioRxiv - Immunology 2021Quote: ... Cell lysates were incubated with anti-FLAG or anti-Myc antibodies in the presence of protein G or A magnetic beads (Thermofisher Scientific) for 2 hours at 4°C while slowly rotating ...
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bioRxiv - Cancer Biology 2019Quote: ... and labeled on ice with 1:250 dilution of secondary antibodies for binding (anti-mouse 488, ThermoFisher A11059 or anti-human 647, ThermoFisher A21445) and expression (anti-chicken 647 ...
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bioRxiv - Biophysics 2019Quote: ... The cells were then washed and incubated for 70 minutes at room temperature with Alexa 488/561-conjugated anti-rabbit and anti-mouse secondary antibodies (Molecular Probes, Invitrogen). The double labeled cells were then washed and kept a 4° C until confocal imaging ...
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bioRxiv - Cell Biology 2021Quote: ... in PBS containing 5% normal goat serum for 30 min at RT followed by incubation with goat anti-chicken and anti-rabbit secondary antibodies (1:400, A-11039, A-11011, Invitrogen, USA) for 30 min at RT ...
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bioRxiv - Neuroscience 2021Quote: Sections were washed in 1x PBS and probed with following secondary antibodies: F(ab’)2-goat anti-mouse/anti-rabbit IgG Alexa 488 (Thermo Fisher), F(ab’)2-goat anti-mouse/anti-rabbit IgG Alexa 555 (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2020Quote: ... Secondary antibodies (Goat anti-rabbit Alexa Fluor 568 and Goat anti-mouse Alexa Fluor 488, Life Technologies, Cat# A11036 and A11029) were applied subsequently for one hour ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies used were goat anti-mouse Alexa Fluor® 488 or goat anti-rabbit Alexa Fluor® 647 (Life Technologies). Cells were sorted on BD FACSCalibur™ and analyzed with FlowJo (v10.6.2).
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bioRxiv - Developmental Biology 2021Quote: Primary antibodies were rabbit anti-eIF4E-5 (1:500; #4524, this work) or rabbit anti-FLAG (1:500; Invitrogen, PA1-984B), and secondary antibodies were Alexa Fluor 488-conjugated anti-rabbit (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were washed twice in TBS-T and secondary antibody solution was added: anti-mouse Alexa-488 and anti-rabbit Alexa-647 (Fisher Scientific) diluted 1/1000 ...
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Empowering Engineered Muscle Function by Extending Connexin 43 Duration with Reduced Graphene OxidesbioRxiv - Bioengineering 2021Quote: ... and those incubated with anti-sarcomeric α-actinin antibody were treated with Alexa Fluor-568 donkey anti-rabbit IgG (1:500) (Invitrogen) and fluorescein (FITC)-conjugated Phalloidin ...
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bioRxiv - Pathology 2021Quote: ... followed by secondary antibody incubation (1hr, RT, goat anti-rabbit alexa 594 and goat anti-mouse alexa 488, 1:500, Fisher Scientific). Stained sections were treated with Sudan Black (0.03% Sudan Black B in 70% ethanol ...
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bioRxiv - Biochemistry 2020Quote: ... or mouse monoclonal anti-N-Cadherin clone GC-4 followed by AlexaFluor 647-conjugated goat anti-mouse antibody (1:200 dilution, ThermoFisher Scientific). Staining was performed for 30 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... were cultured with no antigens (co-stimulation only control) or with anti-CD3/anti-CD28 antibodies bound to magnetics beads (25 μl/106 cells, Thermo Fisher) (positive ...
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bioRxiv - Microbiology 2020Quote: ... They were then washed 3 times with PBS and incubated with goat anti-mouse conjugated to Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (Thermo Fisher) containing DAPI in immunofluorescence buffer for an additional incubation of 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed three times with PBS and then stained with the secondary antibodies goat anti-rabbit IgG Alexa Fluor 488 (Themo Fisher) and goat anti-mouse IgG Alexa Fluor 648 (Thermo Fisher) in PBS containing 1% BSA and 0.3% Triton X-100 for 1 hour in the dark at room temperature (Table S2) ...
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bioRxiv - Immunology 2021Quote: ... EMCV infected cells were washed three times with PBS and then stained with the secondary antibodies goat anti-rabbit IgG Alexa Fluor 488 (Themo Fisher) and goat anti-mouse IgG Alexa Fluor 648 (Thermo Fisher) in PBS containing 1% BSA and 0.3% Triton X-100 for 1 hour in the dark at room temperature ...
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bioRxiv - Immunology 2020Quote: ... the bound antibodies were detected by incubation with horseradish peroxidase (HRP)-conjugated anti-mouse IgG or anti-Macaca fascicularis IgG antibody (1:5,000, Thermo Fisher Scientific) for 30 min at 37°C ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 virus was labelled using first an anti-SARS-CoV-2 antibody (Spike protein, Thermo Fischer) and then a secondary anti-rabbit Alexa 647 (Thermo Fisher).
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bioRxiv - Developmental Biology 2020Quote: ... secondary antibody staining was performed with Alexa Fluor 568- or 488-labeled detection reagents (goat anti-rabbit, goat anti-mouse antibodies; Molecular Probes) at 1:200 dilution ...
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bioRxiv - Immunology 2021Quote: ... Naïve CD8+ T cells were then activated in vitro using Dynabeads™ conjugated with anti-CD3 and anti-CD28 monoclonal antibodies (Gibco), for a total of 48h ...
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bioRxiv - Neuroscience 2022Quote: ... biotin-conjugated secondary antibodies (Biotin-XX Goat anti-Mouse IgG B2763, or Biotin-XX Goat anti-Rabbit IgG B2770, Thermo Fisher) were utilized at a dilution of 1:750 ...
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bioRxiv - Neuroscience 2022Quote: ... Alexa Fluor 488 conjugated goat anti-chicken (A-11039) and Alexa Fluor 546 conjugated goat anti-rabbit (A-11035) secondary antibodies (1:200 dilution, ThermoFisher Scientific) were used to visualize fluorescence signals ...
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bioRxiv - Immunology 2022Quote: ... The secondary antibody mixture contained anti-rabbit AlexaFluor-555 and anti-mouse AlexaFluor-488 (all purchased from ThermoFisher Scientific; 1:250). Nuclear counterstaining was performed using SlowFade Gold antifade reagent with DAPI (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with a 1:200 dilution of Alexa Fluor 647 goat anti-mouse (A21235) or Alexa Fluor 488 donkey anti-rabbit (A21206) secondary antibodies (Molecular Probes) for 1h in a humidity chamber at room temperature ...
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Single-cell assessment of trophoblast stem cell-based organoids as human placenta-modeling platformsbioRxiv - Developmental Biology 2022Quote: ... sections and coverslips were washed with PBS and incubated with Alexa Fluor goat anti-rabbit 568 and goat anti-mouse 488 conjugated secondary antibodies (1:200; A11036, A32723; Life Technologies) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... The supernatants that contain the eluted targets were analyzed by immunoblotting with anti-Ub (ubiquitin, PTM-1107, PTM-BIO) and anti-RFP antibodies (MA5-15257, Thermo Scientific).
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bioRxiv - Microbiology 2022Quote: ... Immunostaining of NS1 and TRIM25 was realized by using the following antibody combination: mouse anti-NS1/goat anti-mouse Alexa 488 (ThermoFisher Scientific) and rabbit anti-TRIM25 (abcam)/goat anti-rabbit Atto647N (sigma) ...
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bioRxiv - Microbiology 2022Quote: ... For determination of infection rates employing wide field fluorescence microscopy the following antibodies in combination with a DAPI counter stain were used: mouse anti-nucleoprotein/goat anti-mouse Alexa488 (ThermoFisher Scientific). Cells grown on cover slips were mounted on microscopy slides using Mowiol.
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bioRxiv - Neuroscience 2022Quote: ... Membranes were then incubated for 2h at room temperature with HRP-coupled secondary antibodies (anti-rabbit HRP, Proteintech; anti-mouse HRP, Thermofisher Scientific) diluted from 1/1000 to 1/10000 in blocking solution ...
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bioRxiv - Molecular Biology 2023Quote: ... The anti-FLAG antibody was detected using an anti-mouse Alexa 647 secondary antibody (Alexa 488 goat anti-mouse, 1:500 dilution, RT, 1 h, Invitrogen, #A11029). The images were captured with an InCell2000 imager.
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bioRxiv - Neuroscience 2022Quote: ... tissue was washed in blocking solution and treated with secondary antibody (anti-guinea pig 488 1:50 and anti-chicken 594 1:300, Invitrogen, WA316328) for 2-3 days ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then washed 3 × 5 min in PBS at room temperature and incubated in secondary antibody solution (Dk anti Rb AF 488, 1:500, Jackson ImmunoResearch; Gt anti Ms AF 633, 1:500, ThermoFisher) for 2 hrs at room temp ...
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bioRxiv - Bioengineering 2024Quote: ... Alexa Fluor 488 and 568 conjugated goat anti-rabbit or anti-rat IgG antibodies (1:500 each; Invitrogen; Carlsbad, CA, USA) were used for fluorescent signal detection ...
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bioRxiv - Biophysics 2024Quote: ... Samples were washed 3 times for 5 minutes with PBS and incubated for 2 hours at room temperature with secondary antibodies anti-mouse AF488 and anti-rabbit AF561 (Thermo Fisher) diluted 1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... coverslips were washed three times in PEMBALG and incubated with Alex Fluor 488 goat anti-rabbit and/or Alex Fluor 594 goat anti-mouse antibodies (1:1000; Life Technologies) for four hours at room temperature ...