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Citations for Thermo Fisher :
5601 - 5650 of 10000+ citations for Anti NKCC1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed in PBS-T and incubated with fluorescent secondary antibody (anti-mouse or anti-rabbit conjugated to AlexaFluor488 or 647, both Life Technologies) for 2-3h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated with secondary antibody (AlexaFluor 546 donkey anti-mouse for PV, goat anti-rabbit for GluR2/3 or NPY, both 1:400, Thermo Fisher). Secondary antibody was dissolved in 1% normal donkey serum for PV or 1% normal goat serum for GluR2/3 or NPY ...
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bioRxiv - Cell Biology 2023Quote: ... 40D4 Mouse mAb #2920, dilution 1/2000), anti-pAKT (Cell Signalling Technology, rabbit Antibody #9271, dilution 1/1000),anti-ERK (Thermo-Fisher Scientific: Invitrogen, mouse antibody #10221703 ...
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bioRxiv - Cell Biology 2023Quote: ... 40D4 Mouse mAb #2920, dilution 1/2000), anti-pAKT (Cell Signalling Technology, rabbit Antibody #9271, dilution 1/1000),anti-ERK (Thermo-Fisher Scientific: Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... 1:10, anti-mouse Minus-probe, 1:10, and anti-chicken Alexa Plus 647-secondary antibody, 1:1,000, Thermo Fisher Scientific) at 37°C for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... were used as primary antibodies and Goat anti-rabbit Alexa 647 and goat anti-guinea pig Alexa 568 (Thermo Fisher Scientific) were used as secondary antibodies ...
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bioRxiv - Genomics 2023Quote: ... cells were incubated with a secondary antibody (Anti-Rabbit Alexa Fluor-568: Invitrogen A10042; Anti-Mouse Alexa Fluor-488: Invitrogen #A11001) at 1:500 dilution in PBS-T for 1 hour at RT and then washed three times in PBS-T ...
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated for 1 h at RT with the secondary antibodies: goat anti-rabbit immunoglobulin G (IgG) Alexa Fluor 488 and/or goat anti-mouse IgG Alexa Fluor 594 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 37.5 µg affinity purified anti-CDCA7 or anti-HELLS antibodies was coupled to 150 µl Protein A Dynabeads (Thermo Fisher Scientific) and used to deplete 100 µl extract at 4 °C for 45 min ...
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bioRxiv - Genetics 2023Quote: ... cells were incubated for 1 hour at room temperature with secondary antibodies (Alexa555 - donkey anti-rabbit and Alexa 488 - donkey anti-mouse, 1:2000, Thermofisher Scientific) and DAPI diluted in the same buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were then washed 2X for 10 minutes in PBS1X and incubated for 1h at room temperature with blocking solution containing the following fluorescent secondary antibodies: anti-mouse IgG Alexa Fluor 488 and anti-rabbit IgG Alexa Fluor 594 (Thermo Fisher) diluted 1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies were: alexa-488 and alexa-546 -conjugated goat anti-mouse IgG or anti-rabbit IgG (Molecular Probes, Life Technologies). Nuclear counterstaining using DAPI (4’,6-diamino-2-phenylindole ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies were: alexa-488 and alexa-546 -conjugated goat anti-mouse IgG or anti-rabbit IgG (Molecular Probes, Life Technologies). Nuclear counterstaining using DAPI (4’,6-diamino-2-phenylindole ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were washed in DPBS for 10 min and incubated with secondary antibodies (Donkey anti-rabbit Life Technologies cat. no A31573, 1:400; Donkey anti-rat ThermoFisher Scientific cat ...
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Treg cells drive MYCN-mediated immunosuppression and tumor aggressiveness in high-risk neuroblastomabioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies included horseradish-peroxidase-conjugated anti-mouse or anti-rabbit IgG (1:2000 or 1:5000; ThermoFisher, 31430 or 31460). Proteins were detected by ECL chemiluminescence detection kits (ThermoFisher ...
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bioRxiv - Genetics 2023Quote: ... the sections were washed three times with PBS 1X and then incubated for 1 hour with donkey anti-mouse (or anti-rabbit) Alexa Fluor 488nm (or 555nm) secondary antibodies (1:200, Thermofisher Scientific). DAPI (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were incubated for 40 min at RT with Alexa Fluor 488 nm anti-rabbit or Alexa Fluor 594 nm anti-mouse secondary antibodies (Life Technologies). Finally ...
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bioRxiv - Cancer Biology 2024Quote: ... and HRP-conjugated rabbit anti-mouse secondary antibody (#31430) and HRP conjugated Goat anti-mouse IgM (#31440) for immunoblotting were from Thermo Fisher. APC conjugated anti-human ganglioside GD2 antibody (#357306 ...
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bioRxiv - Microbiology 2024Quote: ... of skimmed milk powder and then incubated for 2 h at RT or overnight at 4 °C in 3 ml of PBS 1X + 0.1% (v/v) Tween-20 in presence of a 1,000x dilution of anti GFP antibody (Rabbit anti-GFP, Invitrogen Thermo Fisher Scientific). After washing ...
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bioRxiv - Neuroscience 2024Quote: ... The donkey anti-mouse IgG AlexaFluor 564 and donkey anti-chicken AlexaFluor 488 antibodies were purchased from Life Technologies (Carlsbad, CA).
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bioRxiv - Cancer Biology 2024Quote: ... blocked in 5% normal serum in 1× PBS Triton 0.3% for 1 hr at room temperature and then incubated with the primary antibody (anti-S100b diluted 1:1000, Abcam, ab5264 or anti-RPE65 diluted 1:250, Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... secondary antibodies donkey anti-rabbit IgG and donkey anti-mouse IgG conjugated with Alexa Fluor 488 and 647 (Thermo Fisher Scientific), respectively ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were fixed and immunolabeled with anti-GFP primary Ab (Aves, GFP-1020) and AlexaFluorTM 594 goat-anti-chicken secondary antibody (Invitrogen, A11042). Fields of view tiling almost the entire coverslip were acquired with a BZ-X810 Keyence system (Keyence corporation of America ...
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bioRxiv - Microbiology 2024Quote: ... Colon tissue sections were prepared for immunofluorescence staining as previously described [27] using goat anti-human-AF647 and rabbit anti-GFP-AF488 conjugated antibodies (ThermoFisher, Invitrogen).
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bioRxiv - Genomics 2024Quote: ... and incubated with either anti-rabbit or anti-rat secondary antibodies with AF594 (diluted 1:400, A11012 or A1107, Thermofisher Scientific) for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... visualized with AF488-conjugated anti-rabbit secondary antibody (1:300 from stock; Goat anti rabbit AF488, Thermo Fisher Scientific A11034, RRID:AB_2576217). Slides were imaged on an ANDOR Dragonfly microscope (Oxford Instruments ...
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bioRxiv - Cell Biology 2019Quote: ... clone K124C (1:10 dilution) served as primary antibody and goat anti-mouse Alexa Fluor™594 antibody (1:500 dilution; Invitrogen, Thermo Fisher Scientific) as the secondary antibody ...
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bioRxiv - Biochemistry 2020Quote: ... washed three times with PBS and added with the secondary antibody (Alexa Fluor 488 Conjugated anti-mouse antibody, Life technologies by ThermoFisher Scientific) at 1:250 for overnight at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... cells were washed and incubated with secondary antibodies dependent on the primary antibody (Alexa 555 (Thermo Fisher Scientific, goat anti-mouse 1: 500) and Alexa 488 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... goat anti-rabbit IgG secondary antibody conjugated to Alexa Fluor 568 and goat anti-mouse IgG secondary antibody conjugated to Alexa Fluor 568 (Invitrogen, dilution 1:500). Overall ...
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bioRxiv - Cell Biology 2021Quote: ... cells were permeabilised with phosphate buffer for 2 hours and then incubated with self-made anti-HA antibody (1:500) followed by Alexa488-conjugated secondary antibody (1:2,000) (Life Technologies, Thermo Fisher Scientific A11008). Specimens were mounted with Roti®-Mount FluorCare DAPI (Carl Roth ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-SUMO2/3 antibody (MBL) was labelled with Alexa Fluor® 594 using an Alexa Fluor™ 594 Antibody Labeling Kit (Invitrogen-ThermoFischer) and diluted to 1/200 in Can-Get-SignalTM Immunostain solutions (TOYOBO) ...
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bioRxiv - Neuroscience 2021Quote: ... After five times washing cycles with Wash Solution 2 for 30 min at RT the secondary antibody (1:200 in Antibody Diluent, goat anti-rabbit Alexa Fluor 568; Molecular Probes, Eugene, USA) was applied for 24 h (4 °C) ...
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bioRxiv - Microbiology 2020Quote: ... After sodiumcitrate pressure cooking, the rabbit anti-nucleocapsid antibody (NovusBio, code: NB100-56683SS, dilution 1:100) and an AlexaFluor-488 secondary antibody (ThermoFisher, code: A-11034) as well as the ProLong™ Gold Antifade Mountant with DAPI (ThermoFisher ...
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bioRxiv - Cancer Biology 2021Quote: ... with the appropriate secondary antibodies and the same anti-MCL-1 primary antibody (1:1000) and Clean-Blot™ IP Detection Reagent (HRP) (Thermo Scientific #21230) was used as secondary antibody (1:1000).
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bioRxiv - Neuroscience 2020Quote: ... GFP was amplified with an anti-chicken GFP antibody from Abcam and retina slices were incubated overnight with primary antibodies in blocking solution followed by washes in PBS and a 3-hour incubation with anti-isotypic secondary antibodies (Invitrogen or Jackson Immunoresearch). Labeling of Dact2-GFP or Plcxd2-GFP retinas for GAD67 (mouse monoclonal ...
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bioRxiv - Neuroscience 2019Quote: ... Vglut2a:DsRed] larval brains were stained using activated caspase-3 antibody followed by anti-rabbit IgG Cy5 conjugated antibody (Molecular Probes, A-10523,1:300) and imaged as previously described ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were then incubated overnight at 4 °C in 1:100 (in antibody diluent) anti-cadherin-11 antibody (CDH-11, rabbit polyclonal, Thermo Fisher, 71-7600). Cells were washed three times with PBS-tween and incubated in the conjugated secondary antibody ...
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bioRxiv - Immunology 2021Quote: ... Biotinylated and unconjugated primary antibodies were detected by incubating with Streptavidin-PE (Pharmingen, 534061) and Rat anti-mouse IgG2a antibody (Invitrogen, 17-4210-80) respectively ...
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bioRxiv - Neuroscience 2020Quote: ... secondary antibody incubation was carried out using a 1:1000 dilution of Alexa 647 conjugated donkey anti-goat antibody (Life Technologies, Carlsbad, CA) overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... including an incubation in the donkey anti-goat Alexa 488-conjugated secondary antibody as indicated above and then in a donkey anti-rabbit Alexa 546-conjugated secondary antibody (A10040, 1:500; Life Technologies, Madison, WI) overnight at 4°C.
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bioRxiv - Developmental Biology 2022Quote: ... Binding of the primary antibody was visualized with secondary donkey anti-rabbit antibody conjugated with AlexaFluor® 555 (1:1000, Molecular Probes, Invitrogen). UB branches and the collecting ducts were stained with FITC-conjugated DBA (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed with PBS three times and incubated with antibody solution with Alexa 568-conjugated anti-mouse IgG antibody (Molecular Probes, A-11019) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed several times with PBS and subsequently incubated with secondary antibody with the following antibodies in blocking solution for 30-60 minutes: donkey anti-rabbit Alexa Fluor 488 (Themo Fisher Scientific; A-21206), donkey anti-mouse Alexa Fluor 555 (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Slices were then incubated with appropriate secondary antibody: goat anti-mouse Alexa Fluor 594 secondary antibody (1:500, cat. # A21125, Thermo Fisher Scientific [RRID:AB_141593]), goat anti-rabbit Alexa Fluor 647 secondary (1:500 ...
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bioRxiv - Neuroscience 2023Quote: Secondary antibodies: Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (Alexa Fluor 488, Thermo Fisher Scientific, A-21202, 1:1000), Donkey anti-Mouse IgG (H+L ...
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bioRxiv - Developmental Biology 2024Quote: ... the sections were incubated first with anti-Ybx1 primary antibody and then Alexa Fluor 488/568-conjugated secondary antibody (Life Technologies, Carlsbad, CA). The sections were counterstained for nuclei with Hoecyst33342 ((Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... using a rabbit anti-WCTD antibody (GeneScript) (1:100) and Alexa Fluor 647 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary antibody (Invitrogen, Cat# A-31573) (1:500) ...
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Laser Scanning Confocal printing, a new simple method for micro-device fabrication (LSCprint method)bioRxiv - Bioengineering 2024Quote: ... After washing, incubation 30’ with secondary antibodies and DAPI (Termofisher, D1306) was performed: an Alexa 488 anti-mouse antibody (Thermo Fisher A-21202) was used to stain PH3-Ser 10 ...
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bioRxiv - Microbiology 2023Quote: ... followed by the incubation with secondary antibody (HRP-conjugated goat anti-mouse antibody (1: 10,000; NA931V, Cytiva) or HRP-conjugated goat anti-rabbit antibody (1: 10,000; A-11036, Thermo Fisher Scientific, Waltham, MA, USA). The immunoreactive bands were visualized using Chemi-Lumi One Super Kit (Nacalai Tesque ...