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Citations for Thermo Fisher :
5801 - 5850 of 10000+ citations for Anti NKCC1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... They were then incubated at 4 °C overnight with primary antibody rabbit anti-Myosin VI (1:400 Proteus Bioscience) and mouse anti-green fluorescent protein (Invitrogen, cat. A11120). The tissues were incubated with 1:800 diluted secondary antibodies for 2 hrs at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... then immersed in diluted blocking buffer containing Alexa-Fluor-conjugated secondary antibodies (Goat anti-Rabbit and Goat anti-Mouse IgG1, Invitrogen / Thermo-Fisher), each at 1:800 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were rinsed and incubated in the secondary antibody cocktail (1:200, anti-mouse; Alexa-594, Cat. # A11005 and 1:200, Alexa-488 anti-rabbit, Cat. # A11008; ThermoFisher Scientific, USA) for 4- 5hrs at room temperature ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with the appropriate secondary antibodies (anti-rabbit-A488 Life Technologies, 1:1000; anti-mouse-A568 Life Technologies, 1:1000) for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies were Alexa Fluor 488 and Alexa Fluor 594 donkey anti-rabbit and donkey anti-goat (all Life Technologies; 1:300), Alexa Fluor 594-conjugated streptavidin (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibodies used for hiPSC-derived neurons and microglia were as follows: anti-rabbit or anti-mouse antibodies conjugated with Alexa Fluor 488 (Invitrogen, 1:1000), anti-rabbit or anti-mouse antibodies conjugated with Alexa Fluor 555 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... The secondary antibodies used were as follows: Alexa Fluor 488 either anti-mouse or anti-rabbit IgG (A11001, A21206, Thermo Fisher Scientific), Alexa Fluor 647 either anti-mouse or anti-rabbit IgG (A21236 ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa Fluor 546 goat anti-rabbit IgG and Alexa Fluor 546 goat anti-mouse IgG secondary antibodies were purchased from Invitrogen (Carlsbad, CA).
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bioRxiv - Immunology 2023Quote: ... for 20 minutes at 4°C and stained in permwash buffer with anti-cytokine antibodies for one hour at room temperature (anti-IFN-γ PE (XMG1.2, ThermoFisher RRID: AB_466193) or Brilliant violet 785 (XMG1.2 ...
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bioRxiv - Immunology 2023Quote: ... After three washes of blocking solution, cells were stained with goat anti-mouse (AlexaFluor594, A11005) and anti-rabbit (AlexaFlour488, A11034) antibodies (Invitrogen, 1:1,000) for 1h at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... Yeast cells were incubated with 1:1000 dilution of mouse anti-Myc antibody and 1:1000 dilution of chicken anti-His (Invitrogen, PA1-9531) antibodies on ice for 20 min ...
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bioRxiv - Cancer Biology 2023Quote: ... and for 30 min with secondary Alexa488/647-conjugated antibodies (anti-mouse-/anti-rabbit-IgGs: Thermo Fisher Scientific, A-21202/A-31573). Sections were imaged using an Evos FL microscope and marker expression was quantified using CellProfiler 63.
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bioRxiv - Microbiology 2023Quote: ... Cells were then washed and exposed to anti-guinea pig or anti-mouse AF405- or AF488-conjugated secondary antibodies (1:500, Thermo Fisher Scientific) for one hour ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa Fluor conjugated secondary antibodies (Alexa Fluor 488 conjugated goat anti-guinea pig, and goat anti-rabbit were from Invitrogen, MA, USA) were used at 1:500.
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bioRxiv - Neuroscience 2024Quote: ... neurons were incubated with primary antibodies (Rabbit-anti-GFP: 1:1000, Thermo Fisher Scientific, Cat. #G10362; mouse-anti-c-myc: 1:200, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... sections were washed three times with PBS and incubated with Alexa 488 (labeled anti-mouse or rat, 1:400) or Alexa 594 (labeled anti-rabbit, 1:400)-conjugated secondary antibody (Invitrogen, Waltham, MA) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... neurons were washed with PBS three times for 5 min and incubated with secondary antibodies (goat anti-rabbit-AlexaFluor488: 1:1000, Thermo Fisher Scientific, Cat. #A-11034; goat anti-mouse-AlexaFluor488: 1:1000, Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... The sections were then washed three times for five minutes in PBS followed by the corresponding AlexaFluor secondary antibodies (anti-mouse 568—A11031, Thermo Fisher, anti-rabbit 568—A11011, Thermo Fisher, anti-mouse 488—A11001 ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by incubation with the anti-rat and anti-rabbit IgG secondary antibody conjugated to Alexa Fluor 488 and 546 (1:400, Thermo Fisher Scientific) overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... the embryos were stained with secondary antibodies with Alexa 488 goat anti-rat IgG and Alexa 594 goat-anti-mouse IgG (1:250, Invitrogen, Carlsbad, CA) in blocking buffer overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were then washed three times in TBS-T and incubated for 1 h at RT with goat anti-rabbit or goat anti-mouse HRP conjugated secondary antibodies (31460 and 31430, respectively, Thermo Fisher Scientific) diluted 1:10000 in TBS-T ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with fluorescent secondary antibodies (goat anti-rabbit Alexa Fluor 488, and/or goat anti-mouse Alexa Fluor 488; Invitrogen, 1:1,000), and finally washed with PBS three times for 5 min each ...
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bioRxiv - Cancer Biology 2023Quote: ... Fluorophore tagged secondary antibody (Alexa Fluor 568 anti-mouse IgG of Invitogen, A-11004 and Alexa Fluor 488 anti-rabbit IgG of Invitrogen, A-11001) were used in 1:200 dilution and incubated for 1 hrs at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then washed with PBS with 0.03% NaN3 for 15 mins RT × 4 and incubated in secondary antibodies at 1:1000 dilution (goat anti-mouse 488, Thermo Fisher Scientific# A-28175, goat anti-rabbit 647, Thermo Fisher Scientific# A-27040 ...
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bioRxiv - Cancer Biology 2023Quote: ... or 53BP1 clone 6B3E10 (Santa-Cruz) and visualized using Alexafluor-488-conjugated goat anti-rabbit and Alexfluor-546-conjugated goat anti-mouse antibodies (Invitrogen™, Life technologies) along with 4′,6-diamidino-2-phenylindole ...
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bioRxiv - Immunology 2024Quote: ... Horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse secondary antibodies were used to assess protein signal (Thermo Scientific, Cell Signaling Technologies). Chemiluminescence was measured using an Odyssey Fc Imaging System (Licor ...
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bioRxiv - Microbiology 2024Quote: ... of skimmed milk powder and then incubated for 2 h at RT or overnight at 4 °C in 3 ml of PBS 1X + 0.1% (v/v) Tween-20 in presence of a 1,000x dilution of anti GFP antibody (Rabbit anti-GFP, Invitrogen Thermo Fisher Scientific). After washing ...
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bioRxiv - Systems Biology 2024Quote: ... Cells were washed and incubated with either an AF488-conjugated goat anti-rabbit or an AF488-conjugated goat anti-mouse secondary antibody as appropriate (1:300, Thermo-Fisher Scientific, UK) in 1 % FCS ...
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bioRxiv - Developmental Biology 2024Quote: ... Dissociated cells were blocked in 6% BSA and immunolabeled with anti-α3 antibody (mouse Anti-ATP1A3 xVIF 9-G10, MA3-915, Invitrogen, Thermo Fisher scientific) for 2 hours 200 times in PBS1x containing 1%BSA or at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... re-probed for 1 hour with 1:5000 HRP-conjugated secondary antibodies (donkey anti-goat, Santa Cruz #sc-2020; goat anti-rabbit, Invitrogen #31462), washed three times for 5 min with TBST ...
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bioRxiv - Biophysics 2024Quote: ... and stained with 1:100 chicken anti- Myc (Novus) primary antibody followed by 1:500 Alexa Fluor plus 594 goat anti-chicken (Thermo Fisher Scientific) secondary antibody to label Piezo1Myc channels and 1:500 rabbit anti- HA (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2024Quote: ... Cross-absorbed secondary antibodies Alexa Fluor™ 568nm- and 647nm-conjugated goat anti-rabbit and anti-mouse (Life Technologies #A11004 and #A21240) were used at 1/600 ...
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bioRxiv - Neuroscience 2024Quote: ... the same procedure was followed but using anti-β-Tubulin (1:1000, DSHB, USA) as the primary antibody and Goat anti-Mouse AF 546 (1:300, Invitrogen, Germany) as secondary antibody.
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bioRxiv - Immunology 2024Quote: ... for one hour and incubated overnight with the primary antibodies (rabbit-anti-Iba-1, 1:200; Wako, USA; rat-anti- mCherry, 1:2000, Thermo Fisher Scientific). After washing in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... donkey anti-mouse Alexa Fluor 546 (1:500) or goat anti-rabbit Alexa Fluor 488 (1:500) secondary antibodies (Thermo Fisher Scientific). For staining with anti-centromere antibody ...
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bioRxiv - Microbiology 2024Quote: ... Sections were rinsed before incubation with a 1:1,000 dilution of an appropriate Alexa Fluor 546–conjugated (catalog A11018) or A488-conjugated (catalog A11070) goat anti-mouse or anti-rabbit antibody (Thermo Fisher Scientific). After a final wash with PBS ...
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bioRxiv - Cell Biology 2024Quote: ... rinsed 3 times in PBT and incubated for 4 h at room temperature with anti-mouse or anti-rat secondary antibody coupled to Dylight 488 (Thermo Fisher Scientific), or an secondary alpaca nanobody anti-mouse 488 SMS1AF488-1-100 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... membranes were then incubated in appropriate secondary antibodies (goat anti-rabbit-HRP, Invitrogen G21234, 1:10,000; goat anti-mouse IgG1, Invitrogen PA174421, 1:5,000) for 30 minutes at room temperature before washing and enhanced chemiluminescence with (SuperSignal™ West Femto Maximum Sensitivity Substrate ...
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bioRxiv - Cell Biology 2021Quote: ... The following dilutions of antibodies were used in this study: 1/250 anti-Pub1 pS188 and 1/500 anti-Pub1 (custom made by Thermo Scientific, anti-Pub1 being non-specific to pS188) in PBS buffer ...
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bioRxiv - Neuroscience 2021Quote: ... anti-rabbit HRP antibody (1:5000) or with anti-goat HRP antibody (1:5000) was performed for 1 h and ECL (Pierce, Thermo Scientific, Rockford, IL, USA) visualized detection ...
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bioRxiv - Cell Biology 2019Quote: ... clone K124C (1:10 dilution) served as primary antibody and goat anti-mouse Alexa Fluor™594 antibody (1:500 dilution; Invitrogen, Thermo Fisher Scientific) as the secondary antibody ...
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated for 2 hours in anti-mouse antibodies labelled with Alexa Fluor 488 and anti-guinea pig antibodies labelled with Alexa Fluor 568 (1:150) (Life Technologies, Carlsbad, CA, USA). Imaging and data processing were the same for Fly retina immunostaining.
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibody incubation was performed at room temperature for 2h with Alexa 488 anti-mouse IgG-conjugated antibodies (Molecular Probes Inc., Eugene, OR, USA). Cell nuclei were stained with DAPI (Molecular Probes) ...
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bioRxiv - Bioengineering 2020Quote: ... (primary Rabbit anti-mouse alpha-SMA antibody; Cat. No. ab5694; Abcam; 1:50 dilution; secondary Goat anti-rabbit antibody; Cat. No. A11008; Life Technologies; 1:150 dilution). Fluorescent images were acquired using a Leica SP8 confocal microscope ...
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bioRxiv - Cell Biology 2021Quote: ... cells were permeabilised with phosphate buffer for 2 hours and then incubated with self-made anti-HA antibody (1:500) followed by Alexa488-conjugated secondary antibody (1:2,000) (Life Technologies, Thermo Fisher Scientific A11008). Specimens were mounted with Roti®-Mount FluorCare DAPI (Carl Roth ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibody staining was performed by incubation with matching Alexa Fluor 488-conjugated anti-rabbit antibody (Molecular Probes; Cat no.: A11008; Dilution 1:400) and Alexa Fluor 594-conjugated anti-mouse antibody (Molecular Probes ...
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bioRxiv - Bioengineering 2022Quote: ... followed by incubation with secondary antibodies for 1 hour by using an Alexa Fluor-488 anti-mouse IgG antibody (Thermo Fisher Scientific, 1:250), Alexa Fluor-488 anti-rat IgG antibody (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... The following commercially available monoclonal antibodies were used in this study: monoclonal mouse anti-myc antibody (Invitrogen, 132500; IB 1:2’000, IF 1:50), monoclonal mouse anti-HA antibody (Enzo Life Sciences AG ...
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bioRxiv - Neuroscience 2020Quote: ... every 10th section was processed immunohistochemically for ChAT using the same antibody as above and a donkey anti-goat Alexa 594 secondary antibody (Invitrogen/Thermofisher catalogue numbers: A-11058). Motoneurone counts were made manually using an epifluorescent microscope (Axioplan 2 ...
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bioRxiv - Physiology 2020Quote: ... A goat anti-rabbit IgG secondary antibody (Alexa Fluor Plus 488 Highly Cross-Adsorbed Secondary Antibody; 1:400 diluted in PBTD; Fisher Scientific, Stockholm, Sweden; A32731) was added for 2 hours followed by washing in PBTD for 25 minutes ...