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Citations for Thermo Fisher :
5651 - 5700 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... The upper layer was transferred to a 2 mL tube PVDF filter (ThermoFisher, F2520-5) and centrifuged for 5 min at 4,000 × g at 4°C ...
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bioRxiv - Bioengineering 2019Quote: ... Washed cells were incubated in blocking solution containing 5% normal horse serum (Invitrogen, cat. # 31874) and 0.1% triton-X (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 µl were directly loaded on a native polyacrylamide gel (6% DNA Retardation, Thermo Fisher) and run at 4 °C using 0.5 X TBE buffer for 60 min ...
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bioRxiv - Genetics 2020Quote: ... for 5 min prior to mounting in Prolong Gold antifade mounting medium (ThermoFisher Scientific, USA).
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bioRxiv - Synthetic Biology 2019Quote: ... and MIT_v2.1_SbfInifJ_RV2 5’-AACCTGCAGGGCTAACTAACTAACCACGGACAAAAAACC-3’) and ligated into pCR Blunt II TOPO (Thermo Fisher Scientific). The second half containing nifBQFUSVWZM was amplified with SbfI sites on either end (with oligos MIT_v2.1_SbfInifB_FW 5’-AACCTGCAGGTACTCTAACCCCATCGGCCGTCTTA-3’ ...
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bioRxiv - Neuroscience 2019Quote: ... and in the case of NOCL1 and NOCL2 also 5% fetal calf serum (ThermoFisher Scientific). NGN1 expression was induced by addition of doxycycline (2 μg/ml ...
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bioRxiv - Physiology 2019Quote: RNA was isolated from WT and DKO livers (n=5 per group) using TRIzol (Invitrogen), reverse-transcribed ...
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bioRxiv - Cancer Biology 2019Quote: Microscopy images were obtained after dye incubation and 5 minutes of NucBlue (Molecular Probes R37605) staining ...
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bioRxiv - Biophysics 2019Quote: ... 5% CO2 atmosphere in Dulbecco’s modified Eagle’s medium with 1 g/mL glucose (Gibco 31885023) supplemented with 1% penicillin-streptomycin (Sigma P0781) ...
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bioRxiv - Immunology 2020Quote: PBMCs were incubated overnight (37°C, 5% CO2) in X-vivo 15 media (Fisher Scientific) supplemented with 5% AB Serum (Invitrogen ...
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bioRxiv - Physiology 2020Quote: ... and MitoSOX Red mitochondrial superoxide indicator (5 μM, 30 minutes) (Molecular Probes, Invitrogen, Paisley, UK), respectively ...
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bioRxiv - Physiology 2020Quote: ... and MitoSOX Red mitochondrial superoxide indicator (5 μM, 30 minutes) (Molecular Probes, Invitrogen, Paisley, UK), respectively ...
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bioRxiv - Synthetic Biology 2020Quote: ... the cells were mounted with 5 µL of ProLong antifade reagent (Thermo Fisher Scientific, USA), covered with cover glass ...
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bioRxiv - Cell Biology 2019Quote: ... Itgβ1 (TS2/16), SNX6 (Clone D-5; 365965; Santa Cruz Biotechnology, PMP70 (PA1-650; Invitrogen), WASH1 (gifted from D.D ...
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bioRxiv - Developmental Biology 2021Quote: ... at 37°C in a HERAcell 150i or 160i 5% CO2 incubator (Thermo Fisher Scientific). Cells were split 1:4 – 1:10 every 2-3 days when approximately 80% confluent and kept in 6-well dishes or uncoated flasks using Trypsin (0.25%)-EDTA (0.02% ...
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bioRxiv - Developmental Biology 2021Quote: ... at 37°C in a HERAcell 150i or 160i 5% CO2 incubator (Thermo Fisher Scientific). Cells were passaged every 4 days at ∼1 x 105 – 2 x 106 cells/mL in uncoated flasks by pelleting at 150g for 5 minutes and resuspending in fresh media.
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bioRxiv - Cell Biology 2021Quote: ... A 4’-5-diamidina-2-phenylindole (DAPI)-containing mounting medium was used (ThermoFisher, cat. 62248). Images were acquired using a spectral Zeiss LSM800 confocal microscope and analyzed with Fiji software (version 2.0.0) ...
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bioRxiv - Neuroscience 2021Quote: ... samples were heated for 5 minutes at 95°C in sample buffer (#NP0007, Invitrogen, USA), and then separated on 4-12% Bis-Tris mini gels (#NP0336 ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were labeled in 5% BSA/TBST with either Streptavidin-HRP (1:10,000, Thermo Fisher), anti-HA peroxidase conjugated antibodies (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... HFFs were transfected with 5 μg total DNA using a Neon electroporation system (ThermoFisher Scientific) and plated on polyacrylamide gels (traction force microscopy ...
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bioRxiv - Molecular Biology 2021Quote: ... Fluorescence signal was dynamically measured by QuantStudio™ 5 Real-Time PCR System (Thermo Fisher). Background-subtracted signals for each monitoring points were further normalized by subtraction of its initial value to make comparison between different conditions (arbitrary unit ...
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bioRxiv - Molecular Biology 2021Quote: ... The qPCR was performed in a QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific) using the following parameters ...
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bioRxiv - Microbiology 2020Quote: ... 106 293T cells were transfected with 5 µg infectious clone using Lipofectamine 2000 (Life Technologies), cells were freeze-thawed after 1 d ...
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bioRxiv - Microbiology 2021Quote: Mouse lung tissue sections were sectioned via cryostat (Thermofisher CryoStar NX70 cryostat, 5 μm/section) and fixed onto glass slides ...
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bioRxiv - Microbiology 2021Quote: ... with 5% HyClone iron-supplemented calf serum (Cytivia, SH30073.03) and 1% penicillin-streptomycin (Invitrogen, 15140122), and were maintained at 37 °C and 5% CO2 ...
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bioRxiv - Genomics 2019Quote: ... Six picomole of siRNA was transfected into 5×104 cells using Lipofectamine RNAiMax (Thermo Fisher) following the reverse transfection procedure in 24-well format ...
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bioRxiv - Biochemistry 2021Quote: ... then fixed with 4% paraformaldehyde containing 5 µg/ml Hoechst 33342 stain (Thermo Fisher, #62249) for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... 150nM of each primer and 5 μl 2X-Jump Start SYBR Green PCR Mix (Invitrogen) in10 μl total volume ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... An anion-exchange HPLC column (BioBasic AX, 150 x 2.1 mm, 5 µM, Thermo Scientific) was used for the chromatographic separation and a 5500 QTrap (Sciex ...
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bioRxiv - Immunology 2021Quote: C-LP cells pooled from 5-7 mice were sorted into TriZol® (Thermo Fisher) and cDNA was generated by using QuantiTect Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Immunology 2020Quote: ... For experiments using OP9 stromal cells: OP9 cells were labeled with 5 μM CFSE (Invitrogen). Immune complexes containing mono-biotinylated lysozyme and anti-sera generated in mice were formed in vitro ...
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bioRxiv - Cell Biology 2019Quote: ... All cells were grown at 37°C in 5% CO2 and maintained in DMEM (Invitrogen) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Systems Biology 2020Quote: ... then re-suspended in 40 μl of 5% mass spectrometry grade acetonitrile (Thermo Fisher Scientific) and 0.5% formic acid ...
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bioRxiv - Plant Biology 2020Quote: ... and 0.05 μl of Platinum Taq DNA Polymerase (5 U/μl, Invitrogen, Carlsbad, CA, USA). Gene expression was evaluated using a modified 2-ΔCT method (Schmittgen and Livak 2008) ...
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bioRxiv - Cancer Biology 2020Quote: ... at 37°Cin a humidified incubator with an atmosphere of 5% CO2 (Thermo fisher scientific). The cells were passaged at a density of 2.0×105 cells in a 25cm2 flask or cryopreserved at a density of 2.0×106/mL.
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bioRxiv - Animal Behavior and Cognition 2019Quote: COS7 cells were maintained at 37°C under 5% CO2 in DMEM media (Life Technologies) supplemented with 10% fetal bovine serum (Atlanta Biologicals) ...
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bioRxiv - Cell Biology 2019Quote: ... 5 µg of RNA from each sample was treated with TURBO™ DNase (ThermoFisher AM2238) followed by clean-up and enrichment of RNA > ∼150 nt using RNA clean and concentrator (Zymo Research #R1013) ...
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bioRxiv - Developmental Biology 2019Quote: For live imaging 3-5 day old flies were dissected in Schneider’s Insect Media (Thermofisher) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Genomics 2021Quote: ... The diluted (1/5) total cDNA was subjected to Taqman gene expression (ThermoFisher Scientific, USA) using the following transcript-specific probes and primers ...
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bioRxiv - Genetics 2021Quote: ... 3×105 U2OS cells were incubated with 5 pmol siRNA using lipofectamine RNAiMAX (Thermo Fisher) in a 12-well tissue culture plate for 2hr ...
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bioRxiv - Cell Biology 2021Quote: ... the caudal disc sections were blocked in 5% normal serum (Thermo Fisher Scientific, 10000 C) in PBS-T (0.4% Triton X-100 in phosphate-buffered saline (PBS)) ...
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bioRxiv - Genetics 2020Quote: ... and 5% (v/v) heat-inactivated fetal bovine serum (FBS) (all supplements were from Invitrogen). Transient transfection was developed with PEI method and BRET assays were performed as previously described (Reyes-Resina et al. ...
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bioRxiv - Microbiology 2020Quote: ... cells were stained with 5 µM of the membrane dye FM4-64 (Invitrogen, cat# LST3166) for 5 min ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the 5’-RACE procedure was performed using the FirstChoice® RLM-RACE Kit (Invitrogen™). cDNA containing the 5’UTR of CD44 from each cell type was amplified using a nested PCR strategy with the primers:
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bioRxiv - Cell Biology 2020Quote: ... 5% CO2 and atmospheric O2 in a humidified incubator (Thermo Fisher Scientific, Waltham, MA, USA). A549 cells were purchased from Beina Biology (Shanghai ...
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bioRxiv - Cell Biology 2020Quote: ... dried and mounted on microscopy glass slides with Prolong Diamond antifade 5 (Thermo Fisher Scientific). Slides were cured overnight at room temperature and imaged the next day on a spinning disk Eclipse Ti2-E inverted microscope (Nikon) ...
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bioRxiv - Biochemistry 2020Quote: ... packed with 5 μm particles with a pore size of 100 Å (Thermo Fisher Scientific) and separated on an analytical C18 100 column of dimensions 500 mm x 75 μm i.d. ...
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bioRxiv - Cell Biology 2021Quote: ... 2.5 μg of purified plasmid DNA was mixed with 5 μl Lipofectamine 3000 (Thermo Fisher) and used for transfection according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Cells were resuspended 48 h post-infection and fixed in 5% formaldehyde in PBS (Gibco) for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... 5-7 μg BACMID DNA was incubated with 4 μL Fugene (Thermo Fisher, Cat# 10362100) in 200 μL of ESF 921 media for 30 minutes at 23°C ...