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Citations for Thermo Fisher :
5551 - 5600 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Sample-probe mixes were analyzed on a QuantStudio 5 Real-Time PCR System (ThermoFisher Scientific). Copy numbers were analyzed using the ΔΔCt method ...
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bioRxiv - Cell Biology 2020Quote: ... DU145 cells were cultured (37°C, 5% CO2) in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... Bacteria were gently mixed with 5 μl of allophycocyanin (APC)-labeled annexin V (Thermo Scientific) and incubated at room temperature for 15 min in the dark ...
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bioRxiv - Biophysics 2021Quote: ... Purification was performed using reverse-phase HPLC (ThermoFisher, Acclaim C8 column, 5 µm, 120 Å), and fractions were tested with MALDI mass spectrometry to verify the purity ...
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bioRxiv - Biophysics 2020Quote: ... and 5 mL of B-PER™ complete bacterial protein extraction reagent (Thermo Scientific #89821) with 2 mM MgCl2 ...
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bioRxiv - Cell Biology 2021Quote: ... Neuro2a cells were transfected with each siRNA for 5 h using Lipofectamine RNAiMAX (Life Technologies) and further incubated for 24-48 h.
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bioRxiv - Cell Biology 2021Quote: All cultures were performed at 37 °C in a 5% CO2 water jacket incubator (ThermoFisher). For methylcellulose colony assays ...
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bioRxiv - Cell Biology 2021Quote: ... we used previously described snRNA probes 5’-end-labeled with Alexa-488 fluorescent dye (Invitrogen) in HEK293T cells (44 ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were then counterstained for 5 minutes with Hoechst 33342 (1μg/mL, Thermo Scientific, 62249). Slides were mounted using Vectashield Antifade Mounting Medium (Vector Laboratories ...
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bioRxiv - Cell Biology 2021Quote: ... and 5 μg psPAX2 were mixed in Opti-MEM reduced serum medium (Gibco, (Waltham, MA)) with 45 μl of TransIT-LT1 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were cultured at 37°C in a humidified 5% CO2 atmosphere in DMEM (GIBCO) supplemented with 10% heat-inactivated FBS (Gibco) ...
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bioRxiv - Developmental Biology 2022Quote: ... spun at 300g for 5 minutes and resuspended in DMEM (Thermo Fisher Scientific, 11965-118) + 5% fetal bovine serum (VWR ...
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MEK inhibitor resistance in lung cancer cells associated with addiction to sustained ERK suppressionbioRxiv - Cancer Biology 2022Quote: ... Samples were denatured by boiling for 5 min in 4X loading buffer (Thermo Scientific, NP0008). Lysates were loaded on 4-12% Bis-Tris NuPage Protein Gels (NuPage ...
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bioRxiv - Bioengineering 2022Quote: ... 4.0 μL cell Tracker Green 5-chloromethylfluorescein diacetate (CMFDA, 10 μM; Thermo Fisher Scientific, Inc.) and 16.0 μL propidium iodide (PI ...
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bioRxiv - Developmental Biology 2022Quote: ... qRT-PCR plates were run using the QuantStudio 5 Real-Time PCR system (Applied Biosystems) with a total reaction volume of 20 μL ...
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bioRxiv - Neuroscience 2022Quote: ... dissolved in a vehicle solution of 5% ethanol in phosphate-buffered saline (PBS; Fisher Scientific), as described32,33 ...
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bioRxiv - Neuroscience 2022Quote: ... After washing, blocking buffer (TBS, 5% NGS, 1%BSA, 0.1% Triton or BlockAid solution (Thermofisher)) was applied for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... 105 cells were inoculated into 5 ml of Dulbecco’s Modified Eagle Medium (DMEM; Life Technologies) in a 6-well tissue culture plate and incubated with 5% CO2 at 37 °C for 5 days.
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bioRxiv - Evolutionary Biology 2022Quote: ... minigenes containing variable 5′ss were inserted in to the pcDNA5/FRT expression vector (Invitrogen). 1 μg of minigene plasmid was then transiently transfected into HeLa cells ...
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bioRxiv - Developmental Biology 2022Quote: ... Reactions were quenched by adding PBS supplemented with 5% fetal bovine serum (FBS, Life technologies). Dissociated cells were centrifuged at 500 g for 5 minutes and cell pellets were washed with PBS- 5%FBS before passing thought a Bel Art™ SP Scienceware™ Flowmi™ 40 μm cell strainers (ThermoFisher Scientific) ...
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bioRxiv - Physiology 2022Quote: Fibers were incubated in 5 µM rhod-2 in the membrane permeable AM form (Invitrogen). Rhod-2 was excited with 531 nm light and the emitted light was collected through a 585 nm long-pass filter.
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bioRxiv - Cell Biology 2022Quote: ... was mixed and incubated with 5 μl NuPAGE™ LDS Sample Buffer (ThermoFisher Scientific, NP0007) and 2 μl 1M DTT (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.25 μL (5 μM) of each primer and 4.25 μL of ultrapure water (Gibco #10977). The cycling conditions included 50°C for 2 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were first trapped (Acclaim PepMap100 C18, 5 μm, 100A; Thermo Fisher Scientific, MA, USA) before being separated on an analytical column (Agilent Poroshell ...
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bioRxiv - Biochemistry 2022Quote: ... the peptides were sampled on a precolumn (300 μm × 5 mm PepMap C18, Thermo Scientific) and separated in a 75 μm × 250 mm C18 column (Reprosil-Pur 120 C18-AQ ...
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bioRxiv - Biochemistry 2022Quote: ... the membranes were blocked with 5% non-fat dry milk in TBST (Thermo Fisher Scientific) and incubated with primary antibodies for the appropriate target ...
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bioRxiv - Cell Biology 2022Quote: Human HEK293 cells were cultured at 37 °C and 5% CO2 in DMEM (ThermoFisher Scientific), supplemented with 10% fetal bovine serum (ThermoFisher Scientific) ...
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bioRxiv - Physiology 2022Quote: ... Each sample was resuspended in 250μL of HBSS containing 5% normal rat serum (Thermo Scientific). Then 2μL of fluorescently labeled anti-TER-119 and streptavidin was added to the suspension ...
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bioRxiv - Immunology 2022Quote: ... The bacteria were grown on blood agar plates (TSA with 5% sheep blood, Thermo Fisher). Inoculated plates were placed into a hypoxia chamber (Billups- Rothenberg) ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 mM EDTA) and settled for 3 min in a magnetic stand (Fisher scientific, #FERMR02). Beads were then resuspended in 1 volume of IP buffer and ready to use ...
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bioRxiv - Molecular Biology 2022Quote: ... Extract was precleared by incubating overnight with 5 ug of mouse polyclonal IgG (Invitrogen, #31903) in the presence of 100 ug RNase A (Fisher scientific ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... RT-PCR was performed in QuantStudio™ 5 Real-Time PCR System (Thermo Fisher Scientific) and calculated using ΔΔCt-method ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Each 10 μL reaction contained 5 μL of PowerSYBR Green PCR Master Mix (Applied Biosystems), forward and reverse primers at a final concentration of 0.2 μM ...
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bioRxiv - Microbiology 2022Quote: ... with the addition of 5% Fetal Clone II serum (Hyclone) and 1X antibiotic/antimycotic (Gibco). Working stock titers were determined via plaque assay by adding serially diluted virus to Vero E6 cell monolayers ...
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bioRxiv - Microbiology 2022Quote: ... Single disks containing either 5 mg BA or 25 μg FLC (6 mm, Fisher Scientific) were placed in the center of the MH plates ...
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bioRxiv - Biochemistry 2022Quote: ... and then incubated with 5 ug/mL Alexa Fluor 350-conjugated Wheat Germ Agglutinin (Invitrogen) in PBS at room temperature for 10 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... 5 µg of plasmid was added to 2.5 mL Opti-MEM Reduced-Serum Medium (Gibco). 15 mg of sterile PEI was added to 2.5 mL Opti-MEM Reduced-Serum Medium ...
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bioRxiv - Biochemistry 2022Quote: ... 10 μl of 5 μM siRNA was diluted into 250 μl of Opti-MEM (Gibco) and 10 μl of Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: Two pieces of each organ (5 mg) were cut and kept in RNAlater (Thermo Fisher) until further processing ...
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bioRxiv - Molecular Biology 2022Quote: ... Grids were blotted for 3 - 5 s in a Vitrobot (Mark IV, Thermo Fisher Scientific) at 20 °C and 100% humidity ...
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bioRxiv - Immunology 2022Quote: ... in a humidified atmosphere at 37°C with 5% CO2 supplemented with 10% FBS (Gibco), penicillin (50 U/ml ...
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bioRxiv - Plant Biology 2022Quote: ... Quantitative RT-PCR was performed using Quant Studio 5 real-time PCR system (Applied Biosystems) and GoTaq qPCR Master Mix (Promega) ...
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bioRxiv - Bioengineering 2022Quote: ... 20% of cells were treated with CellTracker™ Green CMFDA Dye (5:1000, C2925, Invitrogen) and imaged via fluorescence microscopy (Axiovert 200M ...
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bioRxiv - Neuroscience 2022Quote: hOPCs were seeded at 5 × 104 cells/mL and Lipofectamine RNAi max (Invitrogen, #13778-150) was used to transfect cells according to the manufacturer’s protocol with minor alterations ...
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bioRxiv - Cancer Biology 2022Quote: ... 293T cells (5 × 106) were seeded in 150 mm plates with Opti-MEM (Gibco; 51985091). After 24h ...
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bioRxiv - Cell Biology 2022Quote: ... and additionally incubated for 5 minutes with NucBlue (NucBlue Live Cell Stain Ready Probes, Invitrogen), to allow for determination of the nuclear area.
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bioRxiv - Cell Biology 2022Quote: ... centrifuged at 1000 rpm for 5 min at RT and resuspended in HBSS (Fisher Scientific) supplemented with 1% BSA (Carl Roth) ...
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bioRxiv - Cancer Biology 2022Quote: ... BLCa organoid media contains the following reagents: Basis medium containing 5% FBS (Gibco, 1027-106), 1x B27 supplement (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were boiled for 5 min at 95 °C before SDS PAGE (Thermo Fisher Scientific). PVDF membranes were probed with the following primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... Metabolically labeled NSPs were conjugated to tetramethylrhodamine 5-carboxamido-(6-azidohexanyl) (TAMRA-N3, Invitrogen, T10182) via CuAAC ...