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Citations for Thermo Fisher :
5651 - 5700 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... E and S1 exposure were conducted in the presence or absence of neutralizing antibodies against toll-like receptor 2 and 4 (TLR2 and TLR4) antibodies (ThermoFisher Scientific Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... and grown to an OD600 range of 2-4 at 30°C in a deep-well 96-well plate (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: Larval fish (4-7 dpf) were mounted in a small drop of 2% low melting point agarose (ThermoFisher Scientific 16520) in the center of the uncoated side of a mirror galvanometer (Thorlabs GVS0111) ...
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bioRxiv - Molecular Biology 2022Quote: ... endogenous PABP-mRNA complexes were immunoprecipitated from cleared lysates for 2 hours at 4°C using Dynabeads protein G (ThermoFisher) conjugated to anti-PABP (clone 10E10 ...
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bioRxiv - Molecular Biology 2023Quote: ... The gradient was centrifuged at 200.000×g for 2 hours at 4°C in a S52-ST rotor (Thermo Scientific) and subsequently fractionated into 14 fractions using the BioComp Piston Gradient Fractionator device ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were centrifuged at 500 ×g for 5 minutes at 4°C and washed and resuspended with ice-cold FACS Buffer (2 or 10% FBS in 1× HBSS (Gibco) or 1X PBS) ...
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bioRxiv - Cell Biology 2022Quote: ... pMDG2.1-VSV-G and p8.91-GAG-POL in a ratio of 4:1:2 to a total of 10 μg of DNA using Lipofectamine 2000 (ThermoFisher) as per the manufacturer’s instructions overnight ...
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bioRxiv - Bioengineering 2023Quote: ... RPMI 1640 containing 2 μM calcein AM and 4 μM EthD-1 along with 1 mg/mL DAPI (ThermoFisher, 62248) was added to the 384-deep well plate (80 μL/well) ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then pegylated for 15 minutes with 2 mM MS(PEG)4 Methyl-PEG-NHS-Ester (ThermoFisher Scientific) before grids.
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bioRxiv - Biochemistry 2022Quote: ... The filtrate was then incubated for 1 h rotating at 4 °C with 2 mL Ni-NTA resin (Thermo Fisher) per 1.5 L of expression culture ...
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bioRxiv - Genetics 2023Quote: ... Protein was separated on a 4–12% Bis-Tris gel and transferred using iBlot 2 transfer apparatus (Thermo Fisher Scientific). A 5% milk solution in TBST was used for blocking for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were washed with assay buffer (PBS containing 0.2% BSA and 1 mM CaCl2) and resuspended in assay buffer with 2 μM Fluo-4-AM (Life Technologies). Fluo-4-AM was prepared as a 1 mM stock in DMSO ...
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bioRxiv - Bioengineering 2023Quote: ... TUBB4A and MUC5AC overnight at 4 °C and then incubated with Alexa 488-F(ab’)2 fragment of goat anti-mouse IgG (Invitrogen) for 2 h at room temperature ...
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Beclin1-Deficient Adipocytes Promote Tumor Progression by YAP/TAZ-dependent Adipocyte TransformationbioRxiv - Biochemistry 2023Quote: ... Colony formation assays were performed by mixing various numbers of differentiated adipocytes (incubated for 2 days in differentiation media and then 4 days with DMEM (Gibco), 1 μM insulin (I9278 ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated o/n at with rotation at 4°C with 2 µg IP antibody and for 1 h with Dynabeads Protein G (Invitrogen). Alternatively ...
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bioRxiv - Cell Biology 2023Quote: ... The reactions were quenched with 2 μl of 2.7 M ammonium bicarbonate before loading on Bolt™ 4–12% Bis-Tris Plus gels (Invitrogen) for separation ...
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bioRxiv - Biochemistry 2023Quote: ... and 10% 2-Mercaptoethanol or 50 mM dithiothreitol (DTT) and run on NuPAGE 4-12% bis-tris gels (Thermo Fisher). Proteins were transferred to nitrocellulose membranes ...
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bioRxiv - Immunology 2023Quote: ... intracellular staining was performed for 2 h at 4°C after stimulation for 2 h with PMA/Ionomycin Cell Stimulation Cocktail containing protein transport inhibitors (ThermoFisher). Data were acquired on an Aurora (Cytek Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... Antibody–protein complexes were recovered during 2 h incubation at 4°C with protein A/G magnetic beads (Thermo Scientific), followed by duplicate washes of 15 min with low-salt wash buffer (0.1% SDS ...
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bioRxiv - Cancer Biology 2024Quote: ... and A-253 cells were serially diluted and seeded at three different dilutions (2, 4 and 128 cells/well) with 50 µL serum-free DMEM/F12 medium (Gibco) supplemented with 10 ng/ml human EGF (R & D systems) ...
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bioRxiv - Developmental Biology 2024Quote: ... for 2 hrs at RT and incubated overnight at 4°C in primary antibody solution (1:100 rabbit anti-GFP (ThermoFisher) in blocking reagent) ...
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bioRxiv - Immunology 2024Quote: ... filtered through a 0.45 µm pore-sized filter to remove cell debris and concentrated by centrifugation at 100000 g for 2 h at 4°C (Thermofisher WX Ultra80 centrifuge ...
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bioRxiv - Neuroscience 2024Quote: ... microglia were plated on 25 mm coverslips coated with PLL (0.01%) and Collagen IV (4 µg/ml) and loaded with 10 µM Fura-2 AM (Thermo Fisher) with 0.02% Pluronic F-127 (Thermo Fisher ...
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bioRxiv - Genetics 2024Quote: ... HEK293T cells cultured in 15-cm Petri dishes were grown to 60-70% confluency and then transfected with 4 µg of the UTR library of DNA templates (2 µg for each UTR library) using lipofectamine 3000 reagent (Invitrogen). Fourteen hours after transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... 25 μg of protein (or all of the sample for the MDF multiplexing experiment across 2 gels) was loaded into a NuPAGE 4∼12% Bis-Tris 1.5 mm gel (Invitrogen #NP0335) and gel electrophoresis performed ...
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bioRxiv - Microbiology 2024Quote: ... Transfectants were selected with either 4 nM WR99210 (Jacobus Pharmaceuticals; pSLI) or 2 μg/μl blasticidin S (Life Technologies; pSLI2). SLI for selection of parasites with the plasmid integrated into the genome was done as described (Birnbaum et al. ...
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bioRxiv - Systems Biology 2024Quote: ... A subset of APCs was fixed for 30 minutes in 4% PFA and stained with 2 μM BODIPY (Thermo Fisher) and 20 μM Hoechst (Thermo Fisher) ...
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bioRxiv - Plant Biology 2024Quote: ... CrGAP1-4 and CrGDI1-2 were PCR amplified from leaf cDNAs using Phusion high-fidelity DNA polymerase (Thermo Fisher Scientific) and gene-specific oligonucleotides ...
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bioRxiv - Molecular Biology 2024Quote: ... Gradients were ultracentrifuged at 36,000 rpm for 2 hours at 4°C using a TH-641 rotor (Thermo Fisher Scientific) and fractionated on a Piston Fractionator (BioComp).
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bioRxiv - Microbiology 2024Quote: ... For buffered growth mDMEM-DMP was supplemented with 100 mM of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Gibco 15630056).
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bioRxiv - Immunology 2024Quote: ... 2 to 4×106 CD8+ T cells were lysed in the lysis buffer provided and passed through a homogenizer (Invitrogen) before RNA purification following manufacturer instructions ...
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bioRxiv - Biochemistry 2024Quote: ... that was pre-conjugated to Protein A/G beads (Pierce, 88802) for 2 h 4 °C (note: pre-conjugation crosslinked using 20 mM dimethyl pimelimidate (DMP, ThermoFisher) in borate buffer (40 mM boric acid ...
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bioRxiv - Biochemistry 2024Quote: ... that was pre-conjugated to Protein A/G beads (Pierce, 88802) for 2 h 4 °C (note: pre-conjugation crosslinked using 20 mM dimethyl pimelimidate (DMP, ThermoFisher) in borate buffer (40 mM boric acid ...
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bioRxiv - Cell Biology 2024Quote: ... and 2x the concentration of all the other supplements used in R10 medium (see above) and either 2% or 4% of UltraPure Agarose (Invitrogen) dissolved in one part water to achieve different agarose stiffnesses ...
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bioRxiv - Developmental Biology 2024Quote: ... were co-nucleofected into 1×106 primed hiPSCs using the Lonza 4D-nucleofector (“Primary Cell P3” solution, “CM-113” program) and plated on a feeder layer of irradiated CF1 MEFs (Thermofisher, 4×106 MEFs per 6 well plate) at 8×g104 nucleofected cells per well ...
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bioRxiv - Biophysics 2021Quote: ... One liter of Sf9 cells (2 × 106/mL confluence) was infected with 5 mL P3 baculoviral stock obtained following the manufacturer’s instructions (Invitrogen). The medium containing secreted protein was collected on the 4th day (about 72-hour post-infection ...
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bioRxiv - Bioengineering 2021Quote: Each sample (n=5) was placed in a 2 mL centrifuge tube containing 320 μL of collagenase II (Gibco, Thermo Fisher Scientific Inc. ...
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bioRxiv - Bioengineering 2021Quote: ... #2) Sterilize the oxygen sensor by submerging the sensor tip in 70% ethanol (Thermo Fisher Scientific, 64-17-5) in an eppendorf tube for 10 sec ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Three tadpoles per stage (1, 2, 5, 7, and 8 weeks after hatching) were fixed in an RNAlater (Ambion) solution ...
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bioRxiv - Biochemistry 2020Quote: StNFeoB proteins (2-5 µM each) were incubated with a range of substrate (GTP for molecular biology, Thermo Scientific) concentrations (25 µM to 5000 µM) ...
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bioRxiv - Microbiology 2021Quote: ... A trap column (5 μm, 100 Å, 100 μm × 2 cm, Acclaim PepMap 100 nanoViper C18; Thermo Fisher Scientific) and an analytical column (2 μm ...
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bioRxiv - Neuroscience 2020Quote: Time plug-mated Pbx3−/- mice received a single intraperitoneal injection of 5-ethynyl-2’-deoxyuridine (EdU; 0,1mg/g animal; Invitrogen) at E15.5 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated for 5 min with 2 μM LysoSensor Yellow/Blue DND-160 (L7545, Invitrogen/Thermo Fisher Scientific) in extracellular Ringer’s solution at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated for 5 min with 2 μM LysoSensor Yellow/Blue DND-160 (L7545, Invitrogen/Thermo Fisher Scientific) in extracellular Ringer’s solution at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... Cortical astrocytes were plated at a density of 2.5 × 104 to 5 × 104 cells.cm−2 in Minimum Essential Media (Gibco, MEM) supplemented with 10% Horse Serum (Gibco) ...
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bioRxiv - Biochemistry 2022Quote: ... 2 µL of 20 µM siRNA and 5 µL RNAiMAX were diluted in 400 µL OptiMEM (Thermo Fisher Scientific), mixed gently and incubated for 20 min at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: Cells were then counterstained with DAPI (4,6-diamidino-2-2phenylindole) in 5% BSA with 0.1% Triton X-100 and mounted with Prolong Gold Antifade mounting medium (Invitrogen). Images were then acquired with Olympus Fluoview FV1000 confocal microscope system with PLAPON 60X O SC NA 1.40 ...
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bioRxiv - Immunology 2022Quote: ... IgG was eluted from the resin in 5 × 2 mL fractions using IgG elution buffer (Pierce ThermoFisher, catalog #21004). Fractions were buffer exchanged into PBS ...
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bioRxiv - Cell Biology 2022Quote: Isolated colonic crypts or organoids were loaded at RT with Fura-2/AM (5 μm, 2h; Thermo Fisher Scientific) in HBS ...
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bioRxiv - Cell Biology 2022Quote: ... non-IR and IR animals were systemically injected with 0.9% saline containing 0.25 mg per 25 g of body weight of 5-ethynyl-2’-deoxyuridine (EdU, Thermofisher Scientific) followed by a 2 hour or 7 days chase before sacrifice ...